Objective semen analysis : has the target been reached?

Objectivity in assessing critical sperm characteristics such as concentration, motility and morphology, has been the target of many systems and devices. Methods based on physical principles such as turbidimetry, spectrophotometry, laser Doppler technology, are too imprecise or technically too complicated to be applied routinely in the laboratory. Microscopic images providing photographical, cinematographical or video data acquisition followed by manual or computer-assisted image analysis (CASA) suffer from practical and/or device deficiencies, making them tedious and time-consuming, or unreliable because of confounding factors and uncontrolled artefacts. A single-step computer system using visual evaluation of the microscopic field and manual tracking of sperm movement yields more reliable results, but technician expertise remains essential for correct performance. The scientific basis of multiple errors in semen aspiration, chamber quality, data acquisition and analysis is given. Biased interpretations of results or incorrect handling of statistical data are criticised

Antibody of Goat Zona Pellucida-3 (Gzp3) Protein of Mice(Mus Musculus) Block in Vitro Fertilization of Mice as an Animal Model= Antibodi Protein Zona Pelusida-3 Kambing (GZP3) Asal Mencit(Mus Musculus) Mencegah ...

The researchs of immunocontraception have done in ZP3 of several species, but have not been done in ZP3 of goat. In preliminary study, gZP3 protein was effective prohibited of graviditation of mice. The aim of this study was to prove the potency of gZP3 protein to prohibit in vitro fertilization of mice as an animal model. Antibody of gZP3 produced on mice. Immunized mice serum was analyzed using Elisa and Dot blotting method. Antibody of gZP3 supplemented into M-16 media for oocyte incubation, continued with in vitro fertilization. The result showed that antibody titer of immunized mice serum was higher (