Observing Single Molecules Complexing with Cucurbit[7]uril through Nanogap Surface-Enhanced Raman Spectroscopy.

In recent years, single-molecule sensitivity achievable by surface-enhanced Raman spectroscopy (SERS) has been widely reported. We use this to investigate supramolecular host-guest chemistry with the macrocyclic host cucurbit[7]uril, on a few-to-single-molecule level. A nanogap geometry, comprising individual gold nanoparticles on a planar gold surface spaced by a single layer of molecules, gives intense SERS signals. Plasmonic coupling between the particle and the surface leads to strongly enhanced optical fields in the gap between them, with single-molecule sensitivity established using a modification of the well-known bianalyte method. Changes in the Raman modes of the host molecule are observed when single guests included inside its cavity internally stretch it. Anisotropic intermolecular interactions with the guest are found which show additional distinct features in the Raman modes of the host molecule.The authors acknowledge funding from Walters-Kundert Trust, EPSRC (EP/K028510/1, EP/G060649/1, EP/ H007024/1, ERC LINASS 320503), an ERC starting investigator grant (ASPiRe 240629), EU CUBiHOLE grant and the Defence Science and Technology Laboratory (DSTL). S.K. thanks Krebs Memorial Scholarship (The Biochemical Society) and Cambridge Commonwealth Trust for funding.This is the author accepted manuscript. The final version is available from ACS via http://dx.doi.org/10.1021/acs.jpclett.5b0253

Resistance of Staphylococcus aureus Recovered from Infected Foreign Body In Vivo to Killing by Antimicrobials

Because persistence of infections associated with prosthetic material despite the use of appropriate antibioticsis a major clinical problem,the antimicrobial susceptibility of bacteria responsible for a chronic subcutaneous tissue cage infection in rat was investigated ex vivo. Three to 6 weeks after the initiation of infection, suspensions of two strains of Staphylococcus aureus recovered from the foreign body surface and surrounding fluid wereexposed to either oxacillin, vancomycin, fleroxacin, gentamicin, or rifampin. The MBCs of these bacteria were markedly elevated, in most cases 128 to >256 times higher than the MBCof batch culture S. aureus in either logarithmic or stationary phase. Kinetic studies showed the bacteria did not growwhen incubated for 2 h in Mueller-Hinton broth, possibly reflecting dormancy. Their killing wasslow and incompleteby all antibioticsat > 8 times their MIC. These data provide direct evidence of a decreased susceptibility of S. aureus to the killing effect of antimicrobials during chronic foreign body infections in viv

Fibronectin, Fibrinogen, and Laminin Act as Mediators of Adherence of Clinical Staphylococcal Isolates to Foreign Material

Bacterial adherence to polymer surfaces is a required early step in intravenous (iv) device infection. We collected eight strains of Staphylococcus aureus and 19 of coagulase-negative staphylococci from patients with proven iv device bacteremia and studied the role of plasma or connective-tissue proteins in promoting bacterial adherence to polymethylmethacrylate (PMMA) coverslips. Although only a negligible percentage of organisms adhered to albumin-coated PMMA, surface-bound fibronectin significantly promoted adherence of all isolates. Fibrinogen markedly promoted adherence of all S. aureus strains but of only four coagulase-negative strains. Thus, coagulase-negative staphylococci revealed a marked heterogeneity in adherence to fibrinogen-coated surfaces, a result suggesting the existence of heretofore unknown receptors for fibrinogen. Laminin promoted adherence of staphylococci to a much lower extent. Although strain specific, adherence of clinical staphylococcal isolates to foreign surfaces is significantly increased by fibronectin, fibrinogen, and laminin, an observation suggesting the possible contribution of these proteins to the pathogenesis of iv device infectio

Standards for Belief Representations in LLMs

As large language models (LLMs) continue to demonstrate remarkable abilities across various domains, computer scientists are developing methods to understand their cognitive processes, particularly concerning how (and if) LLMs internally represent their beliefs about the world. However, this field currently lacks a unified theoretical foundation to underpin the study of belief in LLMs. This article begins filling this gap by proposing adequacy conditions for a representation in an LLM to count as belief-like. We argue that, while the project of belief measurement in LLMs shares striking features with belief measurement as carried out in decision theory and formal epistemology, it also differs in ways that should change how we measure belief. Thus, drawing from insights in philosophy and contemporary practices of machine learning, we establish four criteria that balance theoretical considerations with practical constraints. Our proposed criteria include accuracy, coherence, uniformity, and use, which together help lay the groundwork for a comprehensive understanding of belief representation in LLMs. We draw on empirical work showing the limitations of using various criteria in isolation to identify belief representations

Still No Lie Detector for Language Models: Probing Empirical and Conceptual Roadblocks

We consider the questions of whether or not large language models (LLMs) have beliefs, and, if they do, how we might measure them. First, we evaluate two existing approaches, one due to Azaria and Mitchell (2023) and the other to Burns et al. (2022). We provide empirical results that show that these methods fail to generalize in very basic ways. We then argue that, even if LLMs have beliefs, these methods are unlikely to be successful for conceptual reasons. Thus, there is still no lie-detector for LLMs. After describing our empirical results we take a step back and consider whether or not we should expect LLMs to have something like beliefs in the first place. We consider some recent arguments aiming to show that LLMs cannot have beliefs. We show that these arguments are misguided. We provide a more productive framing of questions surrounding the status of beliefs in LLMs, and highlight the empirical nature of the problem. We conclude by suggesting some concrete paths for future work

ISSUES IN REFORMING TARIFF-RATE IMPORT QUOTAS IN THE AGREEMENT ON AGRICULTURE IN THE WTO

Contents: The Economics of Tariff Rate Quotas and the Effects of Trade Liberalization; TRQs and GATT Rules; An Overview of Tariffs, Quotas and Imports Worldwide; TRQs in the European Union; U.S. TRQs for Sugar, Tobacco and Peanuts; Dairy TRQs in the United States; Tariff Rate Quota Implementation and Administration by Developing Countries; Management of Tariff Rate Quotas in Korea and Japan; Tariff Rate Quota Administration in Canadian Agriculture; The Case of Australia and New Zealand Facing TRQs; The 1999 WTO Panel Report on the EU's Common Market Organization for Bananas; AssessmentInternational Relations/Trade,

18th International Working Conference on Requirements Engineering: Foundation for Software Quality. Proceedings of the Workshops RE4SuSy, REEW, CreaRE, RePriCo, IWSPM and the Conference Related Empirical Study, Empirical Fair and Doctoral Symposium

This ICB Research Report constitutes the proceedings of the following events which were held during the Requirements Engineering: Foundation for Software Quality (REFSQ) conference 2012 in Essen, Germany. Engineering for Sustainable Systems (RE4SuSy), Requirements Engineering Efficiency Workshop REEW 2012), Creativity in Requirements Engineering (CreaRE 2012), Requirements Prioritization for customer oriented Software Development (RePriCo), International Workshop on Software Product Management (IWSPM), Alive Empirical Study, Online Questionnaires, Empirical Research Fair, Doctoral Symposium

Thermodynamic Theory of Weakly Excited Granular Materials

We present a thermodynamic theory of weakly excited two-dimensional granular systems from the view point of elementary excitations of spinless Fermion systems. We introduce a global temperature T that is associated with the acceleration amplitude \Gamma in a vibrating bed. We show that the configurational statistics of weakly excited granular materials in a vibrating bed obey the Fermi statistics.Comment: 12 pages, 1 figure, To Appear in Phys. Rev. Lett. April, 199

Material reutilization cycles across industries and production lines

The concept of Industrial Symbiosis aims at organizing industrial activity like a living ecosystem where the by-product outputs of one process are used as valuable raw material input for another process. A significant method for the systematic planning of Industrial Symbiosis is found in input–output matching, which is aimed at collecting material input and output data from companies, and using the results to establish links across industries. The collection and classification of data is crucial to the development of synergies in Industrial Symbiosis. Public and private institutions involved in the planning and development of Industrial Symbiosis rely however on manual interpretation of information in the course of creating synergies. Yet, the evaluation and analysis of these data sources on Industrial Symbiosis topics is a tall order. Within this chapter a method is presented which describes value creation activities according to the Value Creation Module (VCM). They are assessed before they are integrated in Value Creation Networks (VCNs), where alternative uses for by-products are proposed by means of iterative input-output matching of selected value creation factors