Hours of high-frequency stimulations reveal intracellular neuronal trends in vivo

The neuronal response to controlled stimulations in vivo has been classically estimated using a limited number of events. Here we show that hours of high-frequency stimulations and recordings of neurons in vivo reveal previously unknown response phases of neurons in the intact brain. Results indicate that for stimulation frequencies below a critical neuronal characteristic frequency, fc, response timings are stabilized to tens-of-microseconds accuracy. For stimulation frequencies exceeding fc the firing frequency is saturated and independent of the stimulation frequency, as a result of random neuronal response failures. This neuronal plasticity, previously shown in vitro, supports a robust mechanism for low firing rates on a network level

Removal of flowers or inflorescences affects ‘Barnea’ olive fruitlet post-anthesis abscission

A typical olive (Olea europaea L.) inflorescence consists of about 20 flowers. However, in many cultivars, only one fruit develops. This is due to massive abscission of flowers and fruitlets, which occurs during the first month after anthesis. In this study, we used the olive cultivar 'Barnea' to characterize the abscission mechanism and to try to increase fruit set by increasing the number of developed fruit per inflorescence. Removing the lateral flowers 3 weeks before anthesis increased fruit set by more than 50%. Removing all inflorescences but one from a branch increased the number of developed fruits from 0.93 to 2.8 during 2017 and from 0.91 to 3.34 fruits per inflorescence in 2018. Sugar quantification in the pistil revealed that starch level is high on the day of anthesis and low 25 days later in abscised as well as in developed fruit. Soluble carbohydrates are low on the day of anthesis, low in abscised flowers/fruitlets 25 days after anthesis and high in developed fruit. Screening the natural variation found in the Israeli germplasm collection revealed that in most cultivars less than one fruit per inflorescence has developed. However, there are unique cultivars with a higher fruit set.</p

Karlova Studánka a geovědní zajímavosti jejího okolí

Import 20/04/2006Prezenční výpůjčkaVŠB - Technická univerzita Ostrava. Fakulta hornicko-geologická. Institut geologického inženýrství (541

Additional file 1: Figure S1. of Ethephon induced oxidative stress in the olive leaf abscission zone enables development of a selective abscission compound

Changes in pectinase activity in the three different AZs during 7 days after ethephon treatment. Pectinase activity in the LAZ (blue line), FAZ2 (black) and FAZ3 (red) in control or ethephon-treated branches is presented. Error bars represent confidence intervals based on 5 samples (p < 0.95). (TIFF 250 kb

Additional file 3: Table S1. of Ethephon induced oxidative stress in the olive leaf abscission zone enables development of a selective abscission compound

Deep sequencing and mapping statistics of the three AZs samples before and 5 days after ethephon treatment. (DOCX 14 kb