A Δ11 desaturase gene genealogy reveals two divergent allelic classes within the European corn borer (Ostrinia nubilalis)

<p>Abstract</p> <p>Background</p> <p>Moth pheromone mating systems have been characterized at the molecular level, allowing evolutionary biologists to study how changes in protein sequence or gene expression affect pheromone phenotype, patterns of mating, and ultimately, the formation of barriers to gene exchange. Recent studies of <it>Ostrinia </it>pheromones have focused on the diversity of sex pheromone desaturases and their role in the specificity of pheromone production. Here we produce a Δ11 desaturase genealogy within <it>Ostrinia nubilalis</it>. We ask what has been the history of this gene, and whether this history suggests that changes in Δ11 desaturase have been involved in the divergence of the E and Z <it>O. nubilalis </it>pheromone strains.</p> <p>Results</p> <p>The Δ11 desaturase gene genealogy does not differentiate <it>O. nubilalis</it> pheromone strains. However, we find two distinct clades, separated by 2.9% sequence divergence, that do not sort with pheromone strain, geographic origin, or emergence time. We demonstrate that these clades do not represent gene duplicates, but rather allelic variation at a single gene locus.</p> <p>Conclusions</p> <p>Analyses of patterns of variation at the Δ11 desaturase gene in ECB suggest that this enzyme does not contribute to reproductive isolation between pheromone strains (E and Z). However, our genealogy reveals two deeply divergent allelic classes. Standing variation at loci that contribute to mate choice phenotypes may permit novel pheromone mating systems to arise in the presence of strong stabilizing selection.</p

Quantifying Condition-Dependent Intracellular Protein Levels Enables High-Precision Fitness Estimates

Countless studies monitor the growth rate of microbial populations as a measure of fitness. However, an enormous gap separates growth-rate differences measurable in the laboratory from those that natural selection can distinguish efficiently. Taking advantage of the recent discovery that transcript and protein levels in budding yeast closely track growth rate, we explore the possibility that growth rate can be more sensitively inferred by monitoring the proteomic response to growth, rather than growth itself. We find a set of proteins whose levels, in aggregate, enable prediction of growth rate to a higher precision than direct measurements. However, we find little overlap between these proteins and those that closely track growth rate in other studies. These results suggest that, in yeast, the pathways that set the pace of cell division can differ depending on the growth-altering stimulus. Still, with proper validation, protein measurements can provide high-precision growth estimates that allow extension of phenotypic growth-based assays closer to the limits of evolutionary selection

Model Guided Application for Investigating Particle Number (PN) Emissions in GDI Spark Ignition Engines

&lt;div class="section abstract"&gt;&lt;div class="htmlview paragraph"&gt;Model guided application (MGA) combining physico-chemical internal combustion engine simulation with advanced analytics offers a robust framework to develop and test particle number (PN) emissions reduction strategies. The digital engineering workflow presented in this paper integrates the &lt;i&gt;k&lt;/i&gt;inetics &amp;amp; SRM Engine Suite with parameter estimation techniques applicable to the simulation of particle formation and dynamics in gasoline direct injection (GDI) spark ignition (SI) engines. The evolution of the particle population characteristics at engine-out and through the sampling system is investigated. The particle population balance model is extended beyond soot to include sulphates and soluble organic fractions (SOF). This particle model is coupled with the gas phase chemistry precursors and is solved using a sectional method. The combustion chamber is divided into a wall zone and a bulk zone and the fuel impingement on the cylinder wall is simulated. The wall zone is responsible for resolving the distribution of equivalence ratios near the wall, a factor that is essential to account for the formation of soot in GDI SI engines. In this work, a stochastic reactor model (SRM) is calibrated to a single-cylinder test engine operated at 12 steady state load-speed operating points. First, the flame propagation model is calibrated using the experimental in-cylinder pressure profiles. Then, the population balance model parameters are calibrated based on the experimental data for particle size distributions from the same operating conditions. Good agreement was obtained for the in-cylinder pressure profiles and gas phase emissions such as NO&lt;sub&gt;x&lt;/sub&gt;. The MGA also employs a reactor network approach to align with the particle sampling measurements procedure, and the influence of dilution ratios and temperature on the PN measurement is investigated. Lastly, the MGA and the measurements procedure are applied to size-resolved chemical characterisation of the emitted particles.&lt;/div&gt;&lt;/div&gt;</jats:p

Evaluación de calidad de agua en un sistema ribereño utilizado para riego agrícola de subsistencia

En la evaluación del impacto de la contaminación en el ambiente, existen diversas herramientas y técnicas que se aplican para conocer la calidad ambiental de los recursos naturales. El objetivo de este trabajo fue evaluar la calidad del agua en un sistema ribereño en San Luis Potosí, México asociado al impacto del uso agrícola, urbano e industrial. Fueron establecidos cinco puntos de muestreo asociado a diversos usos y descargas residuales en donde se evaluaron 28 muestras durante cuatro días de muestro. Estas fueron distribuidas a los 20, 40, 60, 80, 100 y 120 minutos del trayecto determinando con un medidor multiparámetro los sólidos disueltos totales (SDT), temperatura (T), conductividad eléctrica (CE), pH y oxígeno disuelto (OD). El resultado más destacado de las 28 muestras diarias y del total de 112 que los promedios oxígeno disuelto indican condición hipoxia que indica la desaparición de organismos y especies sensibles, concluyendo que el área ribereña requiere un constante monitoreo y diversificación de parámetros para evaluar su condición para actividades agrícolas.In the assessment of the impact of pollution on the environment, there are various tools and techniques that are applied to know the environmental quality of natural resources. The objective of this work was to evaluate water quality in a river system in San Luis Potosí, Mexico associated with the impact of agricultural, urban and industrial use. Five sampling points associated to various uses and residual discharges were established where 28 samples were evaluated during four days of sampling. The total dissolved solids (SDT), temperature (T), electrical conductivity (CE), pH and dissolved oxygen (OD) were distributed at 20, 40, 60, 80, 100 and 120 minutes of the path. The most outstanding result of the 28 daily samples and the total of 112 that dissolved oxygen averages indicate hypoxia condition indicating the disappearance of sensitive organisms and species, concluding that the riparian area requires constant monitoring and diversification of parameters to evaluate agricultural activities.Fil: Alcalá Jáuregui, J. A. Universidad Autónoma de San Luis Potosí. Facultad de AgronomíaFil: Acosta Doporto Geiler, A. Instituto Tecnológico Superior de los Ríos de Balancan (México)Fil: Rodríguez Ortiz, J.C. Universidad Autónoma de San Luis Potosí. Facultad de AgronomíaFil: Hernández Montoya, A. Universidad Autónoma de San Luis Potosí. Facultad de AgronomíaFil: Martínez Carretero, Eduardo. Consejo Nacional de Investigaciones Científicas y TécnicasFil: Filippini, Maria Flavia. Universidad Nacional de Cuyo. Facultad de Ciencias AgrariasFil: Díaz Flores, P. E. Universidad Autónoma de San Luis Potosí. Facultad de AgronomíaFil: Lara Mireles, J. L. Universidad Autónoma de San Luis Potosí. Facultad de Agronomí

Histology of two rice bodies isolated from the stifle of an adult draught horse stallion

In the human and equine species, different kinds of free floating intra-articular particles are related to certain disorders. Osteochondral fragments formed during osteochondrosis dissecans are the most common finding in the equine species, whereas in humans rice bodies due to rheumatoid arthritis are more frequent. Herein we report a third type of floating body inside the stifle of an adult draught horse stallion, in macroscopic appearance similar to articular rice bodies known in humans. As revealed by histologic examination, the two particles consist of polypoid degenerated structures derived from synovial villi. Their formation was probably induced by ischemia

Short--range impurity in the vicinity of a saddle point and the levitation of the 2D delocalized states in a magnetic field

The effect of a short--range impurity on the transmission through a saddle--point potential for an electron, moving in a strong magnetic field, is studied. It is demonstrated that for a random position of an impurity and random sign of its potential the impurity--induced mixing of the Landau levels diminishes {\em on average} the transmission coefficient. This results in an upward shift (levitation) of the energy position of the delocalized state in a smooth potential. The magnitude of the shift is estimated. It increases with decreasing magnetic field $B$ as $B^{-4}$.Comment: LaTeX, 20 page

N-acetylcysteine lacks universal inhibitory activity against influenza A viruses

N-acetylcysteine (NAC) has been recently proposed as an adjuvant therapeutic drug for influenza pneumonia in humans. This proposal is based on its ability to restrict influenza virus replication in vitro and to attenuate the severity of the disease in mouse models. Although available studies were made with different viruses (human and avian), published information related to the anti-influenza spectrum of NAC is scarce. In this study, we show that NAC is unable to alter the course of a fatal influenza pneumonia caused by inoculation of a murinized swine H1N1 influenza virus. NAC was indeed able to inhibit the swine virus in vitro but far less than reported for other strains. Therefore, susceptibility of influenza viruses to NAC appears to be strain-dependent, suggesting that it cannot be considered as a universal treatment for influenza pneumonia

High coercivity cobalt carbide nanoparticles processed via polyol reaction: A new permanent magnet material

Cobalt carbide nanoparticles were processed using polyol reduction chemistry that offers high product yields in a cost effective single-step process. Particles are shown to be acicular in morphology and typically assembled as clusters with room temperature coercivities greater than 4 kOe and maximum energy products greater than 20 KJ/m3. Consisting of Co3C and Co2C phases, the ratio of phase volume, particle size, and particle morphology all play important roles in determining permanent magnet properties. Further, the acicular particle shape provides an enhancement to the coercivity via dipolar anisotropy energy as well as offering potential for particle alignment in nanocomposite cores. While Curie temperatures are near 510K at temperatures approaching 700 K the carbide powders experience an irreversible dissociation to metallic cobalt and carbon thus limiting operational temperatures to near room temperature.Comment: Total 28 pages, 10 figures, and 1 tabl

Debris Disks: Probing Planet Formation

Debris disks are the dust disks found around ~20% of nearby main sequence stars in far-IR surveys. They can be considered as descendants of protoplanetary disks or components of planetary systems, providing valuable information on circumstellar disk evolution and the outcome of planet formation. The debris disk population can be explained by the steady collisional erosion of planetesimal belts; population models constrain where (10-100au) and in what quantity (>1Mearth) planetesimals (>10km in size) typically form in protoplanetary disks. Gas is now seen long into the debris disk phase. Some of this is secondary implying planetesimals have a Solar System comet-like composition, but some systems may retain primordial gas. Ongoing planet formation processes are invoked for some debris disks, such as the continued growth of dwarf planets in an unstirred disk, or the growth of terrestrial planets through giant impacts. Planets imprint structure on debris disks in many ways; images of gaps, clumps, warps, eccentricities and other disk asymmetries, are readily explained by planets at >>5au. Hot dust in the region planets are commonly found (<5au) is seen for a growing number of stars. This dust usually originates in an outer belt (e.g., from exocomets), although an asteroid belt or recent collision is sometimes inferred.Comment: Invited review, accepted for publication in the 'Handbook of Exoplanets', eds. H.J. Deeg and J.A. Belmonte, Springer (2018