193 research outputs found
Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences
In vertebrates, different isoforms of fibroblast growth factor 2 (FGF2) exist, which differ by their N-terminal extension. They show different localization and expression levels and exert distinct biological effects. Nevertheless, genetic inactivation of all FGF2 isoforms in the mouse results in only mild phenotypes. Here, we analyzed mouse FGF2, and show that, as in the human, mouse FGF2 contains CTG-initiated high molecular-weight (HMW) isoforms, which contain a nuclear localization signal, and which mediate localization of this isoform to the nucleus. Using green fluorescent protein-FGF2 fusions, we furthermore observed, that C-terminal deletions disable nuclear localization of the short low-molecular-weight (LMW) 18-kDa isoform. This loss of specific localization is accompanied by a loss in heparin binding. We therefore suggest that, first, localization of mouse FGF2 is comparable to that in other vertebrates and, second, FGF2 contains at least two sequences important for nuclear localization, a nuclear localization sequence at the N terminus which is only contained in the HMW isoform, and another sequence at the C terminus, which is only required for localization of the LMW 18-kDa isofor
High temperature initiation and propagation of cracks in 12%Cr-steel turbine disks
This work aims to study the crack propagation in 12%Cr steel for turbine disks. Creep Crack Growth (CCG) tests on CT specimens have been performed to define the proper fracture mechanics which describes the initiation of the crack propagation and the crack growth behaviour for the material at high temperature. Results have been used to study the occurrence of crack initiation on a turbine disk at the extreme working temperature and stress level experienced during service, and validate the use of C* integral in correlating creep growth rate on the disk component, in case C* is numerically calculated through FEM analysis or calculated by the use of reference stress concept
A probabilistic framework to define the design stress and acceptable defects under combined-cycle fatigue conditions
Abstract Probabilistic routines are necessary to determine the permissible design stress when the operational conditions of traditional gas turbines are further challenged to reach higher performances in terms of flexibility. This paper focuses on the definition of a probabilistic tool aimed to simulate the damage development under combined high and low cycle fatigue conditions considering the stochastic nature of some of the variables introduced in the model. The variables considered in the present study are the long crack threshold Δ K th , LC and the slope of the crack growth law C in the so-called Paris region, the endurance limit Δ σ w 0 and the variability of the applied stress as it cannot be considered a fixed variable in the complex loading scenario of gas turbines. A broad experimental campaign was performed to characterize the material properties in terms of short and long crack resistance under constant and combined cyclic conditions. The data were used to define the average and variance of the materials parameters to feed Montecarlo simulations and, at a target probability of failure, establish the design stress. This work points to determine safety margins which strictly depend on the natural variability of the variables that govern the damage accumulation in the mechanical component
Nuclear localization of mouse fibroblast growth factor 2 requires N-terminal and C-terminal sequences.
In vertebrates, different isoforms of fibroblast growth factor 2 (FGF2) exist, which differ by their N-terminal extension. They show different localization and expression levels and exert distinct biological effects. Nevertheless, genetic inactivation of all FGF2 isoforms in the mouse results in only mild phenotypes. Here, we analyzed mouse FGF2, and show that, as in the human, mouse FGF2 contains CTG-initiated high molecular-weight (HMW) isoforms, which contain a nuclear localization signal, and which mediate localization of this isoform to the nucleus. Using green fluorescent protein-FGF2 fusions, we furthermore observed, that C-terminal deletions disable nuclear localization of the short low-molecular-weight (LMW) 18-kDa isoform. This loss of specific localization is accompanied by a loss in heparin binding. We therefore suggest that, first, localization of mouse FGF2 is comparable to that in other vertebrates and, second, FGF2 contains at least two sequences important for nuclear localization, a nuclear localization sequence at the N terminus which is only contained in the HMW isoform, and another sequence at the C terminus, which is only required for localization of the LMW 18-kDa isoform
Full coherent control of nuclear spins in an optically pumped single quantum dot
Highly polarized nuclear spins within a semiconductor quantum dot (QD) induce
effective magnetic (Overhauser) fields of up to several Tesla acting on the
electron spin or up to a few hundred mT for the hole spin. Recently this has
been recognized as a resource for intrinsic control of QD-based spin quantum
bits. However, only static long-lived Overhauser fields could be used. Here we
demonstrate fast redirection on the microsecond time-scale of Overhauser fields
of the order of 0.5 T experienced by a single electron spin in an optically
pumped GaAs quantum dot. This has been achieved using full coherent control of
an ensemble of 10^3-10^4 optically polarized nuclear spins by sequences of
short radio-frequency (rf) pulses. These results open the way to a new class of
experiments using rf techniques to achieve highly-correlated nuclear spins in
quantum dots, such as adiabatic demagnetization in the rotating frame leading
to sub-micro K nuclear spin temperatures, rapid adiabatic passage, and spin
squeezing
Quantum control of proximal spins using nanoscale magnetic resonance imaging
Quantum control of individual spins in condensed matter systems is an
emerging field with wide-ranging applications in spintronics, quantum
computation, and sensitive magnetometry. Recent experiments have demonstrated
the ability to address and manipulate single electron spins through either
optical or electrical techniques. However, it is a challenge to extend
individual spin control to nanoscale multi-electron systems, as individual
spins are often irresolvable with existing methods. Here we demonstrate that
coherent individual spin control can be achieved with few-nm resolution for
proximal electron spins by performing single-spin magnetic resonance imaging
(MRI), which is realized via a scanning magnetic field gradient that is both
strong enough to achieve nanometric spatial resolution and sufficiently stable
for coherent spin manipulations. We apply this scanning field-gradient MRI
technique to electronic spins in nitrogen-vacancy (NV) centers in diamond and
achieve nanometric resolution in imaging, characterization, and manipulation of
individual spins. For NV centers, our results in individual spin control
demonstrate an improvement of nearly two orders of magnitude in spatial
resolution compared to conventional optical diffraction-limited techniques.
This scanning-field-gradient microscope enables a wide range of applications
including materials characterization, spin entanglement, and nanoscale
magnetometry.Comment: 7 pages, 4 figure
Universal quantum control of two-electron spin quantum bits using dynamic nuclear polarization
One fundamental requirement for quantum computation is to perform universal
manipulations of quantum bits at rates much faster than the qubit's rate of
decoherence. Recently, fast gate operations have been demonstrated in logical
spin qubits composed of two electron spins where the rapid exchange of the two
electrons permits electrically controllable rotations around one axis of the
qubit. However, universal control of the qubit requires arbitrary rotations
around at least two axes. Here we show that by subjecting each electron spin to
a magnetic field of different magnitude we achieve full quantum control of the
two-electron logical spin qubit with nanosecond operation times. Using a single
device, a magnetic field gradient of several hundred milliTesla is generated
and sustained using dynamic nuclear polarization of the underlying Ga and As
nuclei. Universal control of the two-electron qubit is then demonstrated using
quantum state tomography. The presented technique provides the basis for single
and potentially multiple qubit operations with gate times that approach the
threshold required for quantum error correction.Comment: 11 pages, 4 figures. Supplementary Material included as ancillary
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