59 research outputs found
Effect of resveratrol on mitochondrial function: Implications in parkin-associated familiar Parkinson's disease
Mitochondrial dysfunction and oxidative stress occur in Parkinson's disease (PD), but the molecular mechanisms controlling these events are not completely understood. Peroxisome proliferator-activated receptor-gamma coactivator-1α (PGC-1α) is a transcriptional coactivator known as master regulator of mitochondrial functions and oxidative metabolism. Recent studies, including one from our group, have highlighted altered PGC-1α activity and transcriptional deregulation of its target genes in PD pathogenesis suggesting it as a new potential therapeutic target. Resveratrol, a natural polyphenolic compound proved to improve mitochondrial activity through the activation of several metabolic sensors resulting in PGC-1α activation. Here we have tested in vitro the effect of resveratrol treatment on primary fibroblast cultures from two patients with early-onset PD linked to different Park2 mutations. We show that resveratrol regulates energy homeostasis through activation of AMP-activated protein kinase (AMPK) and sirtuin 1 (SIRT1) and raise of mRNA expression of a number of PGC-1α's target genes resulting in enhanced mitochondrial oxidative function, likely related to a decrease of oxidative stress and to an increase of mitochondrial biogenesis. The functional impact of resveratrol treatment encompassed an increase of complex I and citrate synthase activities, basal oxygen consumption, and mitochondrial ATP production and a decrease in lactate content, thus supporting a switch from glycolytic to oxidative metabolism. Moreover, resveratrol treatment caused an enhanced macro-autophagic flux through activation of an LC3-independent pathway. Our results, obtained in early-onset PD fibroblasts, suggest that resveratrol may have potential clinical application in selected cases of PD-affected patients
Relationship between microalbuminuria and cardiac structural changes in mild hypertensive patients
Relationship between the Hemodynamic and Metabolic Actions of Insulin in Man: A Study by Means of Euglycaemic Clamp in Obese Subjects
Il fenetil estere dell'acido caffeico è un potente inibitore della via metabolica 5-lipossigenasica/leucotrienica
Inibizione della via metabolica 5-lipossigenasica/leucotrienica da parte di sostanze antiossidanti di origine naturale
Familial paroxysmal exercise-induced dyskinesia, epilepsy and mental retardation in a family with autosomal dominant inheritance
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