Simultaneous saccharification and fermentation of steam exploded duckweed: Improvement of the ethanol yield by increasing yeast titre

This study investigated the conversion of Lemna minor biomass to bioethanol. The biomass was pre-treated by steam explosion (SE, 210 °C, 10 min) and then subjected to simultaneous saccharification and fermentation (SSF) using CellicÒ CTec 2 (20 U or 0.87 FPU gﰂ1 substrate) cellulase plus b-glucosidase (2 U gﰂ1 substrate) and a yeast inoculum of 10% (v/v or 8.0 ﰀ 107 cells mLﰂ1). At a substrate concentration of 1% (w/v) an ethanol yield of 80% (w/w, theoretical) was achieved. However at a substrate concentration of 20% (w/v), the ethanol yield was lowered to 18.8% (w/w, theoretical). Yields were considerably improved by increasing the yeast titre in the inoculum or preconditioning the yeast on steam exploded liquor. These approaches enhanced the ethanol yield up to 70% (w/w, theoretical) at a substrate concen- tration of 20% (w/v) by metabolising fermentation inhibitors

General analytic predictions of two-field inflation and perturbative reheating

16 pages, 5 figures, Version 2 includes a revised comparison with Meyers and Tarrant [41

Transport equations for the inflationary trispectrum

We use transport techniques to calculate the trispectrum produced in multiple-field inflationary models with canonical kinetic terms. Our method allows the time evolution of the local trispectrum parameters, tauNL and gNL, to be tracked throughout the inflationary phase. We illustrate our approach using examples. We give a simplified method to calculate the superhorizon part of the relation between field fluctuations on spatially flat hypersurfaces and the curvature perturbation on uniform density slices, and obtain its third-order part for the first time. We clarify how the 'backwards' formalism of Yokoyama et al. relates to our analysis and other recent work. We supply explicit formulae which enable each inflationary observable to be computed in any canonical model of interest, using a suitable first-order ODE solver.Comment: 24 pages, plus references and appendix. v2: matches version published in JCAP; typo fixed in Eq. (54

Release of cell wall phenolic esters during hydrothermal pretreatment of rice husk and rice straw

Background: Rice husk and rice straw represent promising sources of biomass for production of renewable fuels and chemicals. For efficient utilisation, lignocellulosic components must first be pretreated to enable efficient enzymatic saccharification and subsequent fermentation. Existing pretreatments create breakdown products such as sugar-derived furans, and lignin-derived phenolics that inhibit enzymes and fermenting organisms. Alkali pretreatments have also been shown to release significant levels of simple, free phenolics such as ferulic acid that are normally esterified to cell wall polysaccharides in the intact plant. These phenolics have recently been found to have considerable inhibitory properties. The aim of this research has been to establish the extent to which such free phenolic acids are also released during hydrothermal pretreatment of rice straw (RS) and rice husk (RH). Results: RS and RH were subjected to hydrothermal pretreatments over a wide range of severities (1.57–5.45). FTIR analysis showed that the pretreatments hydrolysed and solubilised hemicellulosic moieties, leading to an enrichment of lignin and crystalline cellulose in the insoluble residue. The residues also lost the capacity for UV autofluorescence at pH 7 or pH 10, indicating the breakdown or release of cell wall phenolics. Saponification of raw RS and RH enabled identification and quantification of substantial levels of simple phenolics including ferulic acid (tFA), coumaric acid (pCA) and several diferulic acids (DiFAs) including 8-O-4′-DiFA, 8,5′-DiFA and 5,5′-DiFA. RH had higher levels of pCA and lower levels of tFA and DiFAs compared with RS. Assessment of the pretreatment liquors revealed that pretreatment-liberated phenolics present were not free but remained as phenolic esters (at mM concentrations) that could be readily freed by saponification. Many were lost, presumably through degradation, at the higher severities. Conclusion: Differences in lignin, tFA, DiFAs and pCA between RS and RH reflect differences in cell wall physiology, and probably contribute to the higher recalcitrance of RH compared with RS. Hydrothermal pretreatments, unlike alkali pretreatments, release cinnamic acid components as esters. The potential for pretreatment-liberated phenolic esters to be inhibitory to fermenting microorganisms is not known. However, the present study shows that they are found at concentrations that could be significantly inhibitory if released as free forms by enzyme activity

Characterisation of lignocellulosic sugars from municipal solid waste residue.

Municipal solid waste (MSW) contains significant quantities of plant-derived carbohydrates which have the potential to be exploited as a biomass source. This study evaluated the chemical composition and fractionation of MSW water-insoluble organic matter remaining after recycling of other components (MSWR). The organic matter was prepared as a dry, alcohol insoluble residue (MSWR-AIR, comprising w = 6% of original MSW) and size fractionated into fractions A, B, C & D. Carbohydrates were present in all the sub-fractions, comprising up to w = 54%; their complexity was also assessed by FT-IR spectroscopy. The lignin content in the samples ranged from w = 11–22%. The most carbohydrate-rich subfraction (C; w = 4% original MSW) was sequentially extracted to provide information on the likely constituent cell wall-derived polymers, sugar compositions and uronic acid content. The results indicate that approximately w = 25% of the MSWR-AIR comprises glucose, which appears to be mostly cellulosic in origin. The results are discussed in relation to the potential for exploitation

Oxidative stress, a trigger of hepatitis C and B virus-induced liver carcinogenesis

Virally induced liver cancer usually evolves over long periods of time in the context of a strongly oxidative microenvironment, characterized by chronic liver inflammation and regeneration processes. They ultimately lead to oncogenic mutations in many cellular signaling cascades that drive cell growth and proliferation. Oxidative stress, induced by hepatitis viruses, therefore is one of the factors that drives the neoplastic transformation process in the liver. This review summarizes current knowledge on oxidative stress and oxidative stress responses induced by human hepatitis B and C viruses. It focuses on the molecular mechanisms by which these viruses activate cellular enzymes/systems that generate or scavenge reactive oxygen species (ROS) and control cellular redox homeostasis. The impact of an altered cellular redox homeostasis on the initiation and establishment of chronic viral infection, as well as on the course and outcome of liver fibrosis and hepatocarcinogenesis will be discussed The review neither discusses reactive nitrogen species, although their metabolism is interferes with that of ROS, nor antioxidants as potential therapeutic remedies against viral infections, both subjects meriting an independent review.publishersversionPeer reviewe

The inflationary bispectrum with curved field-space

We compute the covariant three-point function near horizon-crossing for a system of slowly-rolling scalar fields during an inflationary epoch, allowing for an arbitrary field-space metric. We show explicitly how to compute its subsequent evolution using a covariantized version of the separate universe or "delta-N" expansion, which must be augmented by terms measuring curvature of the field-space manifold, and give the nonlinear gauge transformation to the comoving curvature perturbation. Nonlinearities induced by the field-space curvature terms are a new and potentially significant source of non-Gaussianity. We show how inflationary models with non-minimal coupling to the spacetime Ricci scalar can be accommodated within this framework. This yields a simple toolkit allowing the bispectrum to be computed in models with non-negligible field-space curvature.Comment: 22 pages, plus appendix and reference

The δN formula is the dynamical renormalization group

We derive the 'separate universe' method for the inflationary bispectrum, beginning directly from a field-theory calculation. We work to tree-level in quantum effects but to all orders in the slow-roll expansion, with masses accommodated perturbatively. Our method provides a systematic basis to account for novel sources of time-dependence in inflationary correlation functions, and has immediate applications. First, we use our result to obtain the correct matching prescription between the 'quantum' and 'classical' parts of the separate universe computation. Second, we elaborate on the application of this method in situations where its validity is not clear. As a by-product of our calculation we give the leading slow-roll corrections to the three-point function of field fluctuations on spatially flat hypersurfaces in a canonical, multiple-field model.Comment: v1: 33 pages, plus appendix and references; 5 figures. v2: typographical typos fixed, minor changes to the main text and abstract, reference added; matches version published in JCA

Optimal bispectrum constraints on single-field models of inflation

We use WMAP 9-year bispectrum data to constrain the free parameters of an 'effective field theory' describing fluctuations in single-field inflation. The Lagrangian of the theory contains a finite number of operators associated with unknown mass scales. Each operator produces a fixed bispectrum shape, which we decompose into partial waves in order to construct a likelihood function. Based on this likelihood we are able to constrain four linearly independent combinations of the mass scales. As an example of our framework we specialize our results to the case of 'Dirac-Born-Infeld' and 'ghost' inflation and obtain the posterior probability for each model, which in Bayesian schemes is a useful tool for model comparison. Our results suggest that DBI-like models with two or more free parameters are disfavoured by the data by comparison with single parameter models in the same class