Spatially explicit assessment of roundwood and logging residues availability and costs for the EU28

Competition for woody biomass between material and energy uses is expected to further increase in the future, due to the limited availability of forest resources and increasing demand of wood for material and bioenergy. Currently, methodological approaches for modeling wood production and delivery costs from forest to industrial gates are missing. This study combines forest engineering, geographically explicit information, environmental constraints and economics in a bottom-up approach to assess cost–supply curves. The estimates are based on a multitude of wood supply systems that were assigned according to geographically explicit forestry characteristics. For each harvesting and transportation system, efficiencies were modeled according to harvesting sites and main delivery hubs. The cost–supply curves for roundwood and logging residues as estimates for current time and for the future (2030) show that there are large regional differences in the potential to increase extraction in the EU28. In most EU Member States, the costs of logging residues extraction increase exponentially already for low levels of mobilization, while extraction of roundwood can be increased to a larger extent within reasonable costs (30–40 $/m3). The large differences between countries in their harvest potential highlight the importance of spatially explicit analyses

Dysferlin Interacts with Tubulin and Microtubules in Mouse Skeletal Muscle

Dysferlin is a type II transmembrane protein implicated in surface membrane repair in muscle. Mutations in dysferlin lead to limb girdle muscular dystrophy 2B, Miyoshi Myopathy and distal anterior compartment myopathy. Dysferlin's mode of action is not well understood and only a few protein binding partners have thus far been identified. Using affinity purification followed by liquid chromatography/mass spectrometry, we identified alpha-tubulin as a novel binding partner for dysferlin. The association between dysferlin and alpha-tubulin, as well as between dysferlin and microtubules, was confirmed in vitro by glutathione S-transferase pulldown and microtubule binding assays. These interactions were confirmed in vivo by co-immunoprecipitation. Confocal microscopy revealed that dysferlin and alpha-tubulin co-localized in the perinuclear region and in vesicular structures in myoblasts, and along thin longitudinal structures reminiscent of microtubules in myotubes. We mapped dysferlin's alpha-tubulin-binding region to its C2A and C2B domains. Modulation of calcium levels did not affect dysferlin binding to alpha-tubulin, suggesting that this interaction is calcium-independent. Our studies identified a new binding partner for dysferlin and suggest a role for microtubules in dysferlin trafficking to the sarcolemma

L’utilizzo delle protesi endoscopiche nella patologia dell’apparato digerente

L’utilizzo di protesi ad introduzione per via endoscopica per patologie dell’apparato digerente sia benigne che maligne ha avuto negli ultimi anni un considerevole sviluppo. Il posizionamento delle endoprotesi è ben tollerato dai pazienti, non necessita di anestesia e comporta rischi relativamente minimi. Le nuove protesi metalliche autoespansibili permettono di risolvere stenosi anche molto serrate senza quasi mai necessità di dilatazione, con riduzione dei rischi che da questa derivano. Viene riportata una revisione dell’esperienza di protesizzazione per patologie dell’apparato digerente e vengono discussi le indicazioni, i limiti e le complicanze, sulla scorta dei dati dalla letteratura internazionale

Purdue Conference on Active Nonproliferation

One major problem with nuclear security measurements involves source identification inthe presence of low signal-to-background ratio. This scenario iscommon to several applications, ranging from radiation identification atportal monitors to radiation source search with unmanned vehicles. In this context of identification of a large variety of sources, including natural and medical sources, sensitive sources of particular interest, but also potentially new/unknown sources for which no reference measurement is available, statistical methods are particularly appealing for their ability to capture the random nature of the measurements. Among them, Bayesian methods form a generic framework allowing for uncertainty quantification and propagation, which is of prime interest for detection (of known and unknown sources), classification, and quantification of smuggled nuclear and radiological materials. We demonstratethe use of Bayesian models for the identificationof mixed gamma sources, measured with organic scintillatorswithinshort acquisition times. We alsocompare the estimation performance using two different materials: liquid EJ-309 and stilbene crystal

Increasing crop production in Russia and Ukraine—regional and global impacts from intensification and recultivation

Russia and Ukraine are countries with relatively large untapped agricultural potentials, both in terms of abandoned agricultural land and substantial yield gaps. Here we present a comprehensive assessment of Russian and Ukrainian crop production potentials and we analyze possible impacts of their future utilization, on a regional as well as global scale. To this end, the total amount of available abandoned land and potential yields in Russia and Ukraine are estimated and explicitly implemented in an economic agricultural sector model. We find that cereal (barley, corn, and wheat) production in Russia and Ukraine could increase by up to 64% in 2030 to 267 million tons, compared to a baseline scenario. Oilseeds (rapeseed, soybean, and sunflower) production could increase by 84% to 50 million tons, respectively. In comparison to the baseline, common net exports of Ukraine and Russia could increase by up to 86.3 million tons of cereals and 18.9 million tons of oilseeds in 2030, representing 4% and 3.6% of the global production of these crops, respectively. Furthermore, we find that production potentials due to intensification are ten times larger than potentials due to recultivation of abandoned land. Consequently, we also find stronger impacts from intensification at the global scale. A utilization of crop production potentials in Russia and Ukraine could globally save up to 21 million hectares of cropland and reduce average global crop prices by more than 3%

826. Transduction of Human Hematopoietic Stem Cells by RD114-TR-Pseudotyped Lentiviral Vectors

HIV-1-derived lentiviral vectors are efficiently pseudotyped by a chimeric envelope (RD114-TR) encoding the extracellular and transmembrane domains of the FLV RD114 glycoprotein fused to cytoplasmic tail (TR) of the MLV 4070A amphotropic glycoprotein. RD114-TR pseudotyped vectors may be concentrated by centrifugation, are resistant to complement inactivation, and are of particular interest for both ex vivo and in vivo gene therapy applications. We carried out a comparative analysis of VSV-G and RD114-TR-pseudotyped lentiviral vectors in transducing human cord blood-derived CD34+ hematopoietic stem/progenitor cells. Transduction efficiency was comparatively analysed in CD34+ cells in liquid culture, in the progeny of CD34+ clonogenic progenitors in semi-solid culture, and in the progeny of CD34+ repopulating stem cells after xeno-transplantation in NOD-SCID mice. In all cases, RD114-TR-pseudotyped vectors transduced hematopoietic cells at lower m.o.i., resulting in lower toxicity and more efficient stable transduction at comparable vector copy number per genome. Potential changes in CD34+ cells transcription profile and phenotype upon transduction with RD114-TR or VSV-G-pseudotyped vectors was investigated by Affymetrix Gene Chips microarray analysis. We found no significant difference in gene expression patterns between mock-RD114-TR and VSV-G-transduced cells. Our study show that the biology of repopulating hematopoietic stem cells and their progeny is not affected by transduction with RD114-TR-pseudotyped lentiviral vectors

Oenothera picensis Phil. subsp. cordobensis W. Dietr.

Sierra de Ancasti: Ruta Nº 66, intersección con el Río Anquincila, entre Taco y Ancastihttps://www.gbif.org/occurrence/2252105851publishedVersio

Urvillea chacoënsis Hunz.

Sierra de Ambato: Falda E., Quebrada de San Jerónimo, a unos 5 km al N.O. de ChumbichapublishedVersio

Sclerophylax kurtzii Di Fulvio

Cueva del Chacho (Km. 1114: ruta nº 74) entre Patquía y Los ColoradosFil: Ariza Espinar, L. Universidad Nacional de Córdoba. Facultad de Ciencias Exactas, Físicas y Naturales; Consejo Nacional de Investigaciones Científicas y Técnicas. Instituto Multidisciplinario de Biología Vegetal; Argentin