Tumor markers in breast cancer - European Group on Tumor Markers recommendations

Recommendations are presented for the routine clinical use of serum and tissue-based markers in the diagnosis and management of patients with breast cancer. Their low sensitivity and specificity preclude the use of serum markers such as the MUC-1 mucin glycoproteins ( CA 15.3, BR 27.29) and carcinoembryonic antigen in the diagnosis of early breast cancer. However, serial measurement of these markers can result in the early detection of recurrent disease as well as indicate the efficacy of therapy. Of the tissue-based markers, measurement of estrogen and progesterone receptors is mandatory in the selection of patients for treatment with hormone therapy, while HER-2 is essential in selecting patients with advanced breast cancer for treatment with Herceptin ( trastuzumab). Urokinase plasminogen activator and plasminogen activator inhibitor 1 are recently validated prognostic markers for lymph node-negative breast cancer patients and thus may be of value in selecting node-negative patients that do not require adjuvant chemotherapy. Copyright (C) 2005 S. Karger AG, Basel

Primary splenic lymphoma presenting with ascites

An 84 year-old gentleman presented with abdominal distension, anorexia and occasional epigastric pain over a four-week period. Blood parameters revealed a hypochromic microcytic anaemia. Both CT and US scan identified ascites and a mass in the left upper quadrant. An ascitic tap was performed identifying bloody ascites and the presence of reactive mesothelial cells on cytology. A subsequent laparotomy and splenectomy was performed. Histology of the resected spleen revealed a Grade 2 follicular lymphoma (Figure 2). The patient had an uneventful postoperative recovery and was well at 6 months follow up. The spleen is an organ with an important immunological function. Primary splenic involvement occurs in less than 1% of non-hodgkin’s lymphoma. Symptoms of primary splenic lymphoma (PSL) include pyrexia, weight-loss, night sweats, generalised weakness and left upper quadrant pain secondary to spleno - megaly. Ascites is a rare presenting feature of PSL. This report illustrates a case of primary splenic lymphoma which poses diagnostic challenges for the pathologist and clinician and ultimately requires definitive splenectomy to confirm a diagnosis

Caesarean Scar Pregnancy: A waiting game...?

The 47th Annual Scientific Meeting of the British Medical Ultrasound Society: Ultrasound 2015, City Hall, Cardiff, Wales, UK, 9 - 11 December 2015Caesarean scar ectopic pregnancy (CSEP) is a rare occurrence in pregnancy, it’s presentation warrants emergent care. While it is the rarest form of ectopic pregnancy, (1:1,800-1:2,216 of all pregnancies), the incidence is increasing (Rotas et al, 2006). This early study highlighted that the increase is most likely due to the growing number of caesareans being performed. In CSEP, the gestational sac (GS) is implanted within the myometrium of a previous caesarean section scar (Rana et al, 2013). This poster discusses a case where the patient initially presented to the Accident and Emergency Department (A+E), with lower abdominal pain. An overview of her case will be outlined, from initial assessment, diagnosis and management to complete resolution. This case highlights the integral role of the transvaginal ultrasound scan (TVS) in conjunction with serial biochemistry in the management of CSEP. Serial biochemistry involves monitoring the pregnancy hormone human chorionic gonadotrophin (hCG)

Analyzing who is relevant to engage in environmental decision-making processes by interests, influence and impact:The 3i framework

Outcomes in participatory environmental processes are strongly affected by choices about who is engaged. Inclusive engagement diversifies the range of interests and perspectives represented, including those from vulnerable and marginalized groups, ultimately contributing to more socially and environmentally sustainable and equitable outcomes. However, existing “stakeholder analysis” methods often bias participant identification away from disenfranchised groups with limited pre-existing interest or influence, instead favoring the most easily accessed and influential stakeholders. This paper draws on research impact theory and practice to propose a new, more inclusive approach, adding impact to the existing interest and influence criteria (which each begin with the letter “i", hence the 3i framework) that are typically used to identify, categorize and prioritize those who are relevant to be included or excluded from engagement processes. As part of this proposed 3i analysis approach, we articulate a new typology of relevant parties to engage in environmental decision-making processes, including: uninterested; uninterested and impacted; uninterested influencers; disinterested, influential and impacted; only interested; interested and impacted; interested influencers; and interested, influential and impacted. Except for the first group, all types of relevant parties should be considered for engagement wherever possible, with participation strategies tailored to their specific 3i profile. The approach was developed and refined through a series of workshops before developing it into a survey instrument that was piloted to gather 3i data efficiently across several national contexts. Survey findings are presented for a case study identifying those relevant to wetland and peatland restoration in a Scottish catchment. If widely adopted, the 3i framework would be the most consequential change in stakeholder analysis methods since the introduction of interest-influence matrices in the 1980s.</p

A longitudinal study of DNA methylation as a potential mediator of age-related diabetes risk

DNA methylation (DNAm) has been found to show robust and widespread age-related changes across the genome. DNAm profiles from whole blood can be used to predict human aging rates with great accuracy. We sought to test whether DNAm-based predictions of age are related to phenotypes associated with type 2 diabetes (T2D), with the goal of identifying risk factors potentially mediated by DNAm. Our participants were 43 women enrolled in the Women's Health Initiative. We obtained methylation data via the Illumina 450K Methylation array on whole blood samples from participants at three timepoints, covering on average 16 years per participant. We employed the method and software of Horvath, which uses DNAm at 353 CpGs to form a DNAm-based estimate of chronological age. We then calculated the epigenetic age acceleration, or Δage, at each timepoint. We fit linear mixed models to characterize how Δage contributed to a longitudinal model of aging and diabetes-related phenotypes and risk factors. For most participants, Δage remained constant, indicating that age acceleration is generally stable over time. We found that Δage associated with body mass index (p = 0.0012), waist circumference (p = 0.033), and fasting glucose (p = 0.0073), with the relationship with BMI maintaining significance after correction for multiple testing. Replication in a larger cohort of 157 WHI participants spanning 3 years was unsuccessful, possibly due to the shorter time frame covered. Our results suggest that DNAm has the potential to act as a mediator between aging and diabetes-related phenotypes, or alternatively, may serve as a biomarker of these phenotypes

Animal welfare research – progress to date and future prospects

peer-reviewedRECORDABSTRACTARTICLE Animal welfare research – progress to date and future prospects OTHER Author(s): L. Boyle 1 , M. Conneely 1 , E. Kennedy 1 , N. O’Connell 2 , K. O’Driscoll 1 , B. Earley 3 , Publication date (Electronic): 26 February 2022 Journal: Irish Journal of Agricultural and Food Research Publisher: Compuscript Keywords: Animal welfare, beef, dairy, pig, poultry, welfare assessment Abstract The welfare status of an animal is dependent on its ability to cope and exist in harmony with its environment, such that good physical and psychological health is maintained. Improving animal welfare is an increasingly important aspect of livestock production systems due, in a large extent, to increased consumer concerns about animal production practices. Animal welfare is an integrated part of quality assurance programmes for sustainable animal production, considering that welfare, health, management, economy, consumer acceptance and environmental impact are interdependent. The major welfare concerns in the livestock industry in recent years relate to the rearing and management of dairy calves, the welfare of the dairy cow, effect of husbandry management procedures on the welfare of beef cattle, rearing of sows in gestation and farrowing crates, and the broiler (meat) chicken sector. The paper will focus on scientific research underpinning these welfare concerns, with a particular focus on research conducted on the island of Ireland

A proposed mechanism for progesterone regulation of trophoblast MMP2 transcription independent of classical progesterone response elements on its promoter

BACKGROUND: Progesterone receptor act as ligand-inducible transcription factor in the respective target cells by binding to specific progesterone response elements in the promoter of the target genes. However, despite the lack of the classical progesterone response elements on matrix-metalloproteinase-2 promoter, progesterone has been shown to decrease the activity of this promoter PRESENTATION OF THE HYPOTHESIS: It has recently been suggested that in addition to interacting with their classical co-activators and co-repressors, progesterone receptor are capable of binding to several transcription factors. By interacting with other classes of transcription factors, progesterone receptor is capable of transcriptional activation through the transcription factors cognate DNA binding site. TESTING THE HYPOTHESIS: Exploring transcription factors and transcription binding sites, interacting with the progesterone receptor in modulation of the matrix-metalloproteinase promoter. IMPLICATIONS OF THE HYPOTHESIS: Identification of additional endogenous progesterone target genes makes it possible to further explore the signaling mechanisms by which the hormone regulates biological actions. Furthermore, the concepts of ligand-driven conformational diversity and selective tissue actions can be exploited in the future for drug development which selectively regulate orphan receptors from the nuclear receptor family

A Tensioned Human Skin Explant Model Used for Preliminary Assessment of Chemexfoliant-Stimulated Bioeffects

A tensioned ex vivo full-thickness human skin explant platform was used to assess the bioeffects arising from application of several commercial chemexfoliation agents. Although such treatments are well-established, and improved understanding of the underlying mechanistic processes continues to emerge, research into the optimum treatments for specific skin types/conditions is still needed for enhanced efficacy while minimizing recovery time. The 3 commercial chemexfoliation agents employed all contained trichloroacetic acid at well-defined concentrations (6, 10, and 20%) and were applied to the explants' stratum corneum. Subsequently, measurements of dermal remodeling factors (COL1A1, ELN, HAS2, HAS3, and procollagen type I) and inflammatory marker (IL-1b) were undertaken using qPCR and immunofluorescent analyses. Statistical analysis of these data facilitated the establishment of benchmarking biological responses to these trichloroacetic acid-containing agents against untreated controls. The performance of an innovative trichloroacetic acid-free chemexfoliation agent was then measured and, upon comparison with the previous benchmarking data, indicated that dermal remodeling factors could be upregulated in fashion comparable with that of the trichloroacetic acid-containing agents but with significant suppression of inflammatory response. Our measurements thus underscore the promise of the tensioned explant over prolonged study periods and also that potentially valuable insights to guide preclinical strategies may be forthcoming from the protocol developed.</p

Analyzing who is relevant to engage in environmental decision-making processes by interests, influence and impact: The 3i framework

\ua9 2024 The AuthorsOutcomes in participatory environmental processes are strongly affected by choices about who is engaged. Inclusive engagement diversifies the range of interests and perspectives represented, including those from vulnerable and marginalized groups, ultimately contributing to more socially and environmentally sustainable and equitable outcomes. However, existing “stakeholder analysis” methods often bias participant identification away from disenfranchised groups with limited pre-existing interest or influence, instead favoring the most easily accessed and influential stakeholders. This paper draws on research impact theory and practice to propose a new, more inclusive approach, adding impact to the existing interest and influence criteria (which each begin with the letter “i", hence the 3i framework) that are typically used to identify, categorize and prioritize those who are relevant to be included or excluded from engagement processes. As part of this proposed 3i analysis approach, we articulate a new typology of relevant parties to engage in environmental decision-making processes, including: uninterested; uninterested and impacted; uninterested influencers; disinterested, influential and impacted; only interested; interested and impacted; interested influencers; and interested, influential and impacted. Except for the first group, all types of relevant parties should be considered for engagement wherever possible, with participation strategies tailored to their specific 3i profile. The approach was developed and refined through a series of workshops before developing it into a survey instrument that was piloted to gather 3i data efficiently across several national contexts. Survey findings are presented for a case study identifying those relevant to wetland and peatland restoration in a Scottish catchment. If widely adopted, the 3i framework would be the most consequential change in stakeholder analysis methods since the introduction of interest-influence matrices in the 1980s

Barriers to accessing perinatal mental health services and suggestions for improvement: qualitative study of women of Black and south Asian backgrounds

Background Maternity outcomes for women from certain ethnic groups are notably poor, partly owing to their not receiving treatment from services. Aims To explore barriers to access among Black and south Asian women with perinatal mental health problems who did not access perinatal mental health services and suggestions for improvements, and to map findings on to the perinatal care pathway. Method Semi-structured interviews were conducted in 2020 and 2021 in the UK. Data were analysed using the framework method. Results Twenty-three women were interviewed, and various barriers were identified, including limited awareness of services, fear of child removal, stigma and unresponsiveness of perinatal mental health services. Whereas most barriers were related to access, fear of child removal, remote appointments and mask-wearing during COVID-19 affected the whole pathway. Recommendations include service promotion, screening and enhanced cultural understanding. Conclusions Women in this study, an underrepresented population in published literature, face societal, cultural, organisational and individual barriers that affect different aspects of the perinatal pathway