A bacterially-expressed recombinant envelope protein from Usutu virus induces neutralizing antibodies in rabbits

Background: Recently, an emerging flavivirus, Usutu virus (USUV), has caused an epidemic among birds in Europe, resulting in a massive die-off in Eurasian blackbirds. Currently found only in Europe and Africa, it can be envisioned that Usutu virus will follow the path of other flaviviruses, like West Nile virus and Zika virus, and will spread via its mosquito vectors and bird hosts to other parts of the world. Several cases of human infections by Usutu virus have already been published. Anticipating this spread, development of an efficacious vaccine would be highly desirable. Method: This study describes the production in E. coli, purification, and refolding of a partial USUV envelope protein. Prior to immunization, the protein was characterized using size exclusion chromatography, transmission electron microscopy and dynamic light scattering, showing the limited presence of virus-like structures, indicating that the protein solution is probably a mixture of mono and multimeric envelope proteins. Results: Immunizations of two rabbits with the refolded E-protein fraction, mixed with a strong adjuvant, resulted in the generation of neutralizing antibodies, as evidenced in an in vitro assay. Discussion: The way forward towards a subunit vaccine against Usutu virus infection is discussed.Microscopic imaging and technolog

Family loyalty and adolescent problem behavior: The validity of the family group effect

Item does not contain fulltextThe present study examined the relation between perceived justice and trust within family relationships and adolescent internalizing and externalizing problem behavior. Data were gathered from the father, the mother, and two of their adolescent children in 288 families. The social relations model was used to assess perceived justice and trust at the family level and the individual level. Adolescent internalizing and externalizing problem behavior turned out to be best predicted at the family level. Adolescents from families with less just or trustworthy climates showed more of these problem behaviors. Also associations between adolescent problem behavior and individual characteristics of the adolescents were found: adolescents who were generally viewed as less just/trustworthy were reported to have more problems. Our findings suggest that processes at different levels of family functioning (i.e., individual, whole-family) should be taken into account when investigating associations between family characteristics and children's outcomes

Charge storage in chloride - depleted chloroplasts

SIGLEAvailable from CEN Saclay, Service de Documentation, 91191 Gif-Sur-Yvette Cedex (France) / INIST-CNRS - Institut de l'Information Scientifique et TechniqueFRFranc