Valorization of Caesalpinia coriaria Fruit Waste to Enhance the Ruminal Mitigation of Greenhouse Gases Production

La editorial tiene los derechos de autor y le solicito a hacer visibles la fecha técnica del artículo.The use of fruits waste from tanniniferous trees represents a new technology that aims to solve or mitigate greenhouse gases emission, and to increase the production of food of animal origin in an ecofriendly manner. This study aims to evaluate the production of methane and carbon dioxide, degradability by in vitro ruminal fermentation in goats diet supplemented with nuts (Caesalpinia coriaria Jacq. Willd.) for adoption by livestock farmers. Condensed tannins (CT) of C. coriaria inclusion rates were 0 (CT0 or control, no CT), 1.5 (CT1.5), 3.0 (CT3), 4.5 (CT4.5) and 6.0% (CT6) of the total mixed ration. All CT treatments reduced (linear, quadratic and cubic; P = 0.001) CH4, CO2 and H2 gases, and had some increasing effect on total biogas production. However, CT3 reduced greenhouse gases and had the highest biogas production. Addition of tannins from cascalote fruit waste (C. coriaria Jacq. Willd.) to goats diet at CT3 level reduced methane production, improved fermentation and ruminal degradability in vitro and has potential to be used as ecofriendly feed or feed additive

Testing the Efficacy of a Multi-Component DNA-Prime/DNA-Boost Vaccine against Trypanosoma cruzi Infection in Dogs

Immunization of dogs with DNA-prime/DNA-boost vaccine (TcVac1) enhanced the Trypanosoma cruzi-specific type 1 antibody and CD8+ T cell responses that resulted in an early control of acute parasitemia and a moderate decline in pathological symptoms during chronic phase. Further improvement of vaccine-induced immunity would be required to achieve clinical and epidemiological benefits and prevent transmission of parasites from vaccinated/infected dogs to triatomines

Trypanosoma cruzi in dogs: electrocardiographic and echocardiographic evaluation, in Malinalco, State of Mexico

Sandra Díaz González-Vieyra1, Ninfa Ramírez-Durán2, Ángel H Sandoval-Trujillo3, Juan C Vázquez-Chagoyán1, Humberto G Monroy-Salazar1, Alberto Barbabosa-Pliego11Research Center of Advanced Studies in Animal Health, Veterinary Husbandry School, 2Medical and Ambiental Microbiology, Research Center of Advanced Studies in Health Science, School of Medicine, Autonomous University of the State of Mexico, Toluca, Mexico; 3Department of Biological Systems, Metropolitan Autonomous University, Xochimilco, Mexico City, MexicoAbstract: Chagas disease caused by Trypanosoma cruzi is an important public health problem in Latin America. Dogs are considered a risk factor for human Chagas disease, a sentinel for T. cruzi infection in endemic regions and an animal model to study pathological aspects of the disease. The potential use of dogs as indicators of human cardiac pathogenicity of local T. cruzi strains has been studied insufficiently. We studied electrocardiographic (EKG) and echocardiographic (ECG) alteration frequencies observed in an open population of dogs in Malinalco, Mexico, and determined if such frequencies were statistically associated with T. cruzi infection in dogs. Animals (n = 139) were clinically examined and owners were asked to answer a questionnaire about dogs’ living conditions. Two commercial serological tests (IHA, ELISA) were conducted to detect anti-T. cruzi serum antibodies. Significant differences between seropositive and seronegative animals in cardiomyopathic frequencies were detected through EKG and ECG (P < 0.05). Thirty dogs (21.58%) were serologically positive to anti-T. cruzi antibodies (to ELISA and IHA assays), of which nine (30%) had EKG and/or ECG alterations. From the remaining 104 (78.42%) seronegative animals, five (4.5%) had EKG and/or ECG abnormalities. Our data support the hypothesis that most EKG and ECG alterations found in dogs from Malinalco could be associated with T. cruzi infection. Considering the dog as a sentinel and as an animal model for Chagas disease in humans, our findings suggest that the T. cruzi strains circulating in Malinalco have the potential to produce cardiomyopathies in infected humans.Keywords: chagas disease, Trypanosoma cruzi, cardiomyopathy, electrocardiography, echocardiography, Malinalco, Méxic

Lipofection improves gene targeting efficiency in E14 TG2a mouse embryonic stem cells

Electroporation has been the method of election for transfection of murine embryonic stem cells for over 15 years; however, it is a time consuming protocol because it requires large amounts of DNA and cells, as well as expensive and delicate equipment. Lipofection is a transfection method that requires lower amounts of cells and DNA than electroporation, and has proven to be effi cient in a large number of cell lines. It has been shown that after lipofection, mouse embryonic stem cells remain pluripotent, capable of forming germ line chimeras and can be transfected with greater effi ciency than with electroporation; however, gene targeting of mouse embryonic stem cells by lipofection has not been reported. The objective of this work was to fi nd out if lipofection can be used as effi ciently as electroporation for regular gene targeting protocols. This context compares gene targeting effi ciency between these techniques in mouse embryonic stem cells E14TG2a, using a gene replacement type vector. No differences were found in gene targeting effi ciency between groups; however, lipofection was three times more effi cient than electroporation in transfection effi ciency, which makes lipofection a less expensive alternative method to produce gene targeting in mouse embryonic stem cells

Antimicrobial resistance of three common molecularly identified pathogenic bacteria to Allium aqueous extracts

The aim of this work was to evaluate the in vitro bacterial inhibition of different types of garlic on Escherichia coli ATCC 25922, Listeria monocytogenes and Staphylococcus aureus. The bacterial strains were molecularly identified using gen 16S rDNA molecular identification. Four different types of garlics were used: 1) white, 2) Japanese, 3) elephant and 3) black, and these were evaluated at two different concentrations (0.25 and 0.125 g/mL) per garlic type. Bioactive compounds present in the garlics were identified using high-performance liquid chromatography coupled to ultraviolet detector (HPLC-UV), and total polyphenols were quantified by the Folin-Ciocalteu technique. The Kirby-Bauber method was used for the bacterial evaluation. Aqueous extract of black garlic had the highest amount of polyphenols 6.26 ± 0.21 mg GAE/mL. The area of inhibition was measured and classified as sensitive, intermediate or resistant. Using the disc diffusion assay, higher concentration (0.25 g/mL) of aqueous extract of white garlic had the highest antibacterial activity area, with 21.46 ± 3.94 mm for L. monocytogenes, 20.61 ± 2.47 mm for S. aureus and 17.83 ± 2.21 mm for E. coli. White garlic had comparable antimicrobial activity as the control (tetracycline at 30 μg) as indicated by the size of the inhibition halos. Based on your results, white garlic can be used as an alternative to synthetic antimicrobials. 1. Introduction Previously, natural products were used to combat diseases, however, with the discovery of synthetic antibiotics, they were gradually replaced [1,52,53]. Natural products used to be the major ingredient of many drugs but pharmaceutical companies have decreased or even eliminated research on natural products [2,42–46,52]. The focus has been on synthetic antibiotics and their indiscriminate use have reduced their effectiveness against infectious diseases in addition to incidences of antibiotics resistance [3,47–50]. Foodborne diseases are still a public health problem worldwide and the causal agents are mainly Escherichia coli O157: H7, Salmonella spp., Listeria monocytogenes, and Clostridium botulinum [4,5]. Bacteria have the ability to adapt to an environment and develop resistance mechanisms, which can be of natural or acquired origin [6,7,51]. Due to the current problems of antibiotics resistance facing the livestock industry, natural products can be viable and economical al ternatives [55,63]. One of such natural

Immune protection against Trypanosoma cruzi induced by TcVac4 in a canine model.

Chagas disease, caused by Trypanosoma cruzi, is endemic in southern parts of the American continent. Herein, we have tested the protective efficacy of a DNA-prime/T. rangeli-boost (TcVac4) vaccine in a dog (Canis familiaris) model. Dogs were immunized with two-doses of DNA vaccine (pcDNA3.1 encoding TcG1, TcG2, and TcG4 antigens plus IL-12- and GM-CSF-encoding plasmids) followed by two doses of glutaraldehyde-inactivated T. rangeli epimastigotes (TrIE); and challenged with highly pathogenic T. cruzi (SylvioX10/4) isolate. Dogs given TrIE or empty pcDNA3.1 were used as controls. We monitored post-vaccination and post-challenge infection antibody response by an ELISA, parasitemia by blood analysis and xenodiagnosis, and heart function by electrocardiography. Post-mortem anatomic and pathologic evaluation of the heart was conducted. TcVac4 induced a strong IgG response (IgG2>IgG1) that was significantly expanded post-infection, and moved to a nearly balanced IgG2/IgG1 response in chronic phase. In comparison, dogs given TrIE or empty plasmid DNA only developed high IgG titers with IgG2 predominance in response to T. cruzi infection. Blood parasitemia, tissue parasite foci, parasite transmission to triatomines, electrocardiographic abnormalities were significantly lower in TcVac4-vaccinated dogs than was observed in dogs given TrIE or empty plasmid DNA only. Macroscopic and microscopic alterations, the hallmarks of chronic Chagas disease, were significantly decreased in the myocardium of TcVac4-vaccinated dogs. We conclude that TcVac4 induced immunity was beneficial in providing resistance to T. cruzi infection, evidenced by control of chronic pathology of the heart and preservation of cardiac function in dogs. Additionally, TcVac4 vaccination decreased the transmission of parasites from vaccinated/infected animals to triatomines

Anatomopathological and histopathological abnormalities in dogs during the acute and chronic phases of <i>T</i>. <i>cruzi</i> infection and disease development (± TcVac4 vaccine).

<p>Anatomopathological and histopathological evaluations were conducted during acute (day 60 pi) and chronic (day 365 pi) phases of infection and disease development. Classification of abnormalities was conducted according to [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003625#pntd.0003625.ref025" target="_blank">25</a>] and [<a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0003625#pntd.0003625.ref039" target="_blank">39</a>], and represented as-, None; +, slight; ++, moderate; and +++, severe; ND, Not determined (animals not included for the study of these parameters).</p><p>Anatomopathological and histopathological abnormalities in dogs during the acute and chronic phases of <i>T</i>. <i>cruzi</i> infection and disease development (± TcVac4 vaccine).</p

TcVac4-induced antibody response in dogs (± <i>T</i>. <i>cruzi</i>).

<p>Dogs were vaccinated with TcVac4 or TrIE only and infected with <i>T</i>. <i>cruzi</i>, as described in Materials and Methods. Shown are sera levels of <i>T</i>. <i>cruzi</i>-specific IgG <b>(A)</b>, IgG1 <b>(B)</b>, and IgG2 <b>(C)</b> antibody subtypes, determined by an ELISA. Dogs given pcDNA3.1/no infection and dogs given pcDNA3.1/<i>T</i>. <i>cruzi</i> were included as negative and positive controls, respectively. The serology time points are described as day -65 = basal response before immunization, day 0 representing antibody response after last immunization but before challenge infection, day 60 post challenge equivalent to acute infection phase, and day 365 post challenge equivalent to chronic disease phase. Each bar represents the absorbance mean value ± standard deviation. Within the same time point, statistical differences (p < 0.05) among groups are shown with different characters above the bars according to Tukey’s test.</p