Pre-plant release enhanced the earlier establishment of Nesidiocoris tenuis in open field tomato

Ο εχθρός της τομάτας, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae) που εισέβαλε στη χώρα μας, πολύ σύντομα έγινε σημαντικός εχθρός τόσο των υπαίθριων όσο και των θερμοκηπιακών καλλιεργειών. Η εξαπόλυση του αρπακτικού Nesidiocoris tenuis (Reuter) (Heteroptera: Miridae) στο φυτώριο έχει βρεθεί ότι μπορεί να συμβάλει στην αντιμετώπιση του T. absoluta σε καλλιέργειες τομάτας στο θερμοκήπιο. Ο στόχος της παρούσας μελέτης είναι να αξιολογηθεί κατά πόσο σε υπαίθρια καλλιέργεια τομάτας, η εξαπόλυση του N. Tenuis στο φυτώριο μπορεί να συμβάλει στην έγκαιρη εγκατάστασή του. Συμπερασματικά, η εξαπόλυση του N. tenuis συνέβαλε ουσιαστικά στην έγκαιρη αποίκηση της καλλιέργειας σε σύγκριση με τα τεμάχια του μάρτυρα. Επίσης, ο ρόλος του ιθαγενούς πληθυσμού του M. pygmaeus πρέπει να λαμβάνεται σοβαρά υπόψη στα προγράμματα ολοκληρωμένης αντιμετώπισης. Η μέθοδος αυτή είναι αποτελεσματική στην πιο έγκαιρη εγκατάσταση του N. tenuis και θα πρέπει να αξιολογηθεί περαιτέρω η συμβολή της στην αντιμετώπιση του Τ. absoluta.The invasive pest of the tomato crops, Tuta absoluta (Meyrick) (Lepidoptera: Gelechiidae), soon after its establishment became a major problem of outdoor and greenhouse tomato crops across the Mediterranean countries. The pre-plant release of the predator Nesidiocoris tenuis (Reuter) (Heteroptera: Miridae) has been found to substantially contribute to its establishment on the crop and efficient control of T. absoluta in greenhouses. The aim of the current study was to assess whether the pre-plant release of N. tenuis could contribute to its earlier establishment. It was concluded that the release of N. tenuis in the nursery contributed substantially in the earlier increase of its numbers in the field in comparison to the control plots. The native populations of M. pygmaeus appeared on the tomato plants before the native populations of N. tenuis. Thus, the conservation of both predators should be taken into consideration in the integrated management strategies against T. absoluta. Therefore, the application of this method in open field tomato crop enhances the earlier establishment of N. tenuis and should be further evaluated in the control of T. absoluta

The American lifestyle-induced obesity syndrome diet in male and female rodents recapitulates the clinical and transcriptomic features of nonalcoholic fatty liver disease and nonalcoholic steatohepatitis

The pathogenesis of nonalcoholic fatty liver disease (NAFLD) and the progression to nonalcoholic steatohepatitis (NASH) and increased risk of hepatocellular carcinoma remain poorly understood. Additionally, there is increasing recognition of the extrahepatic manifestations associated with NAFLD and NASH. We demonstrate that intervention with the American lifestyle-induced obesity syndrome (ALIOS) diet in male and female mice recapitulates many of the clinical and transcriptomic features of human NAFLD and NASH. Male and female C57BL/6N mice were fed either normal chow (NC) or ALIOS from 11 to 52 wk and underwent comprehensive metabolic analysis throughout the duration of the study. From 26 wk, ALIOS-fed mice developed features of hepatic steatosis, inflammation, and fibrosis. ALIOS-fed mice also had an increased incidence of hepatic tumors at 52 wk compared with those fed NC. Hepatic transcriptomic analysis revealed alterations in multiple genes associated with inflammation and tissue repair in ALIOS-fed mice. Ingenuity Pathway Analysis confirmed dysregulation of metabolic pathways as well as those associated with liver disease and cancer. In parallel the development of a robust hepatic phenotype, ALIOS-fed mice displayed many of the extrahepatic manifestations of NAFLD, including hyperlipidemia, increased fat mass, sarcopenia, and insulin resistance. The ALIOS diet in mice recapitulates many of the clinical features of NAFLD and, therefore, represents a robust and reproducible model for investigating the pathogenesis of NAFLD and its progression.NEW & NOTEWORTHY Nonalcoholic fatty liver disease (NAFLD) affects 30% of the general population and can progress to nonalcoholic steatohepatitis (NASH) and potentially hepatocellular carcinoma. Preclinical models rely on mouse models that often display hepatic characteristics of NAFLD but rarely progress to NASH and seldom depict the multisystem effects of the disease. We have conducted comprehensive metabolic analysis of both male and female mice consuming a Western diet of trans fats and sugar, focusing on both their hepatic phenotype and extrahepatic manifestations

Nonabsorbable Antibiotics Reduce Bacterial and Endotoxin Translocation in Hepatectomised Rats

There is increasing evidence that septic complications, occurring after major hepatectomies, may be caused by gram negative bacteria, translocating from the gut. We investigated in rats, the effect of extended hepatectomy on the structure and morphology of the intestinal mucosa as well as on the translocation of intestinal bacteria and endotoxins. We also examined the effect of nonabsorbable antibiotics on reducing the intestinal flora and consequently the phenomenon of translocation by administering neomycin sulphate and cefazoline. Hepatectomy was found to increase translocation, while administration of nonabsorbable antibiotics decreased it significantly. In addition, hepatectomy increased the aerobic cecal bacterial population, which normalised in the group receiving antibiotics. Among the histological parameters evaluated, villus height demonstrated a significant reduction after hepatectomy, while the number of villi per cm and the number of mitoses per crypt, remained unchanged. Our results indicate that administration of nonabsorbable antibiotics presents a positive effect on bacterial and endotoxin translocation after extended hepatectomy, and this may be related to reduction of colonic bacterial load as an intraluminal effect of antibiotics

Bacterial Infections Change Natural History of Cirrhosis Irrespective of Liver Disease Severity

OBJECTIVES: We assessed the prognostic significance of infections in relation to current prognostic scores and explored if infection could be considered per se a distinct clinical stage in the natural history of cirrhosis. METHODS: We included consecutive patients with cirrhosis admitted to a tertiary referral liver unit for at least 48 h over a 2-year period. Diagnosis of infection was based on positive cultures or strict established criteria. We used competing risk analysis and propensity score matching for data analysis. RESULTS: 501 patients (63% male, 48% alcoholic liver disease, median Model of End-stage Liver Disease (MELD)=17) underwent 781 admissions over the study period. Portal hypertensive bleeding and complicated ascites were the commonest reasons of admission. The incidence of proven bacterial infection was 25.6% (60% community acquired and 40% nosocomial). Survival rates at 3, 6, 12, and 30 months were 83%, 77%, 71%, and 62% in patients without diagnosis of infection, vs. 50%, 46%, 41%, and 34% in patients with diagnosis of infection. Overall survival was independently associated with MELD score (hazards ratio (HR) 1.099), intensive care (ITU) stay (HR 1.967) and bacterial infection (HR 2.226). Bacterial infection was an independent predictor of survival even when patients who died within the first 30 days were excluded from the analysis in Cox regression (HR 2.013) and competing risk Cox models in all patients (HR 1.46) and propensity risk score-matched infected and non-infected patients (HR 1.67). CONCLUSIONS: Infection most likely represents a distinct prognostic stage of cirrhosis, which affects survival irrespective of disease severity, even after recovery from the infective episode

Lynx1 and Aβ1–42 bind competitively to multiple nicotinic acetylcholine receptor subtypes

AbstractLynx1 regulates synaptic plasticity in the brain by regulating nicotinic acetylcholine receptors (nAChRs). It is not known to which extent Lynx1 can bind to endogenous nAChR subunits in the brain or how this interaction is affected by Alzheimer's disease pathology. We apply affinity purification to demonstrate that a water-soluble variant of human Lynx1 (Ws-Lynx1) isolates α3, α4, α5, α6, α7, β2, and β4 nAChR subunits from human and rat cortical extracts, and rat midbrain and olfactory bulb extracts, suggesting that Lynx1 forms complexes with multiple nAChR subtypes in the human and rodent brain. Incubation with Ws-Lynx1 decreases nicotine-mediated extracellular signal–regulated kinase phosphorylation in PC12 cells and striatal neurons, indicating that binding of Ws-Lynx1 is sufficient to inhibit signaling downstream of nAChRs. The effect of nicotine in PC12 cells is independent of α7 or α4β2 nAChRs, suggesting that Lynx1 can affect the function of native non-α7, non-α4β2 nAChR subtypes. We further show that Lynx1 and oligomeric β-amyloid1–42 compete for binding to several nAChR subunits, that Ws-Lynx1 prevents β-amyloid1–42–induced cytotoxicity in cortical neurons, and that cortical Lynx1 levels are decreased in a transgenic mouse model with concomitant β-amyloid and tau pathology. Our data suggest that Lynx1 binds to multiple nAChR subtypes in the brain and that this interaction might have functional and pathophysiological implications in relation to Alzheimer's disease

Effects of rhythm and phrase-final lengthening on word-spotting in Korean

A word-spotting experiment was conducted to investigate whether rhythmic consistency and phrase-final lengthening facilitate performance in Korean. Listeners had to spot disyllabic and trisyllabic words in nonsense strings organized in phrases with either the same or variable syllable count; phrase-final lengthening was absent, or occurring either in all phrases or only in the phrase immediately preceding the target. The results show that, for disyllabic targets, inconsistent syllable count and lengthening before the target led to fewer errors. For trisyllabic targets, accuracy was at ceiling, but final lengthening in all phrases reduced reaction times. The results imply that both rhythmic consistency (i.e. regular syllable count) and phrase-final lengthening play a role in word-spotting and, by extension, in speech processing in Korean, as in other languages. However, the results also reflect the language specific role of prosodic cues. First, the cues here were used primarily with disyllabic targets, which were cognitively more demanding to process partly due to their high phonological neighborhood density. Second, the facilitating effect of rhythmic consistency was weak, possibly because strict consistency is not present in spoken Korean. Overall, rhythmic consistency facilitated spotting when targets mapped onto phrases, confirming the importance of phrasal organization in Korean speech processing

High-Added Value Materials Production from OMW: A Technical and Economical Optimization

The extraction of olive oil generates huge quantities of solids and of high organic wastewaters with toxic constituents that have a great impact on land and water environments. Based on a membrane process, authors proposed an alternative method for treatment of olive mill wastewaters (OMWs). In the present paper, a technoeconomic analysis for the implementation of the proposed method in the entire Region of Western Greece (RWG) is presented. This paper takes into account fixed and operational costs, costs for the infrastructure, equipment, land, maintenance, and so forth, considering the treatment of 50,000 tons per harvesting period in the area of RWG. The study showed that the establishment of only one central treatment manufacture could reduce the uncontrolled disposal of OMW. Exploitation of the isolated fractions as manure in fertilizers (nutrients components) or as components in ecological herbicides (phenolics) can depreciate the total cost in a period of about five years

Glucocorticoids regulate AKR1D1 activity in human liver in vitro and in vivo

Steroid 5β-reductase (AKR1D1) is highly expressed in human liver where it inactivates endogenous glucocorticoids and catalyses an important step in bile acid synthesis. Endogenous and synthetic glucocorticoids are potent regulators of metabolic phenotype and play a crucial role in hepatic glucose metabolism. However, the potential of synthetic glucocorticoids to be metabolised by AKR1D1 as well as to regulate its expression and activity has not been investigated. The impact of glucocorticoids on AKR1D1 activity was assessed in human liver HepG2 and Huh7 cells; AKR1D1 expression was assessed by qPCR and Western blotting. Genetic manipulation of AKR1D1 expression was conducted in HepG2 and Huh7 cells and metabolic assessments were made using qPCR. Urinary steroid metabolite profiling in healthy volunteers was performed pre- and post-dexamethasone treatment, using gas chromatography-mass spectrometry. AKR1D1 metabolised endogenous cortisol, but cleared prednisolone and dexamethasone less efficiently. In vitro and in vivo, dexamethasone decreased AKR1D1 expression and activity, further limiting glucocorticoid clearance and augmenting action. Dexamethasone enhanced gluconeogenic and glycogen synthesis gene expression in liver cell models and these changes were mirrored by genetic knockdown of AKR1D1 expression. The effects of AKR1D1 knockdown were mediated through multiple nuclear hormone receptors, including the glucocorticoid, pregnane X and farnesoid X receptors. Glucocorticoids down-regulate AKR1D1 expression and activity and thereby reduce glucocorticoid clearance. In addition, AKR1D1 down-regulation alters the activation of multiple nuclear hormone receptors to drive changes in gluconeogenic and glycogen synthesis gene expression profiles, which may exacerbate the adverse impact of exogenous glucocorticoids