1,737 research outputs found

    Proximate analysis of snakehead fish, Parachanna obscura (Gunther 1861) of the Cross River, Nigeria

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    African snakehead, Parachanna obscura is being considered as an emerging aquaculture candidate in Nigeria. The species is very palatable and has a good fillet quality. Study was conducted on the proximate composition of the species from Cross River, Nigeria. Samples of snakehead were analyzed for protein, fat, moisture, crude fibre and ash contents. The protein content of the species ranged from 21.03-22.03 with mean value of 21.5%. The fat content ranged from 17.1-17.4% with a mean value of 17.2 %. Moisture content ranged from 51.1-52.0 % with a mean value of 51.7%. Ash and crude fibre contents were very low. The protein content of Parachanna obscura compares favourably with that of other members of the family Channidae. The fat content is exceptionally high, making the species a good healing and recuperating agent for post-natal and post-operation patients. We strongly recommend the culture of Parachanna obscura in Nigeria and its meal for growing children

    Perancangan Sistem Informasi Manajemen Rantai Pasok (Supply Chain Management) Untuk Distribusi Pangan Kabupaten Minahasa Tenggara

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    Rendahnya penyediaan informasi yang mendukung pengelolaan hasil pertanian di daerah Minahasa Tenggara akan berpengaruh pada distribusi hasil produksi. Distribusi yang tidak merata dapat menyebabkan stok produk di suatu daerah akan sangat berlebih, dan di daerah lain menjadi sangat kurang, hal ini akan memicu kurangnya keseimbangan harga produk yang beredar dipasaran. Melihat permasalahan yang ada maka perlu dirancang dan diterapkan sistem informasi yang akan memberikan solusi untuk mengumpulkan berbagai informasi hasil pangan daerah-daerah dan dikelola untuk mengetahui seberapa banyak hasil produksi yang harus dikonsumsi, dijual dan bisa didistribusikan ke daerah lain dari petani atau kelompok tani maupun Perusahaan pertanian berbadan hukum. Metode penelitian yang digunakan adalah metode penelitian dan pengembangan (Research and Development). Rancangan sistem dibuat dengan memperhatikan aspek-aspek kebutuhan fungsional dan non-fungsional. Pengujian rancangan menggunakan loop testing untuk menggambarkan pembentukan struktur program secara keseluruhan

    Expression optimisation of recombinant α-Larabinofuranosidase from Aspergillus niger ATCC 120120 in Pichia pastoris and its biochemical characterisation

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    A gene encoding α-L-arabinofuranosidase (AnabfA) from Aspergillus niger ATCC 120120 was successfully cloned and expressed in Pichia pastoris under the control of the AOX1 promoter. The effect of cultural conditions on recombinant AnabfA production was studied and the enzyme was expressed as a soluble protein. Recombinant AnabfA was expressed as an active enzyme at 28°C when cultured in BMMY medium (pH 6.0) and induced with 2% methanol every 24 h. Maximum activity was observed 5 days after induction. The purified recombinant AnabfA before and after treatment with PNGase F migrated by SDS-PAGE had relative molecular masses of about 83 and 66 kDa, respectively, suggesting that the AnabfA contains N-linked oligosaccharides. Characterisation of the purified recombinant AnabfA showed an optimum temperature and pH of 50°C and 4, respectively. The enzyme was stable at a pH of 3 to 6 and retained more than 80% of its activity after pre-incubation at 40°C for 30 min. The recombinant AnabfA activity was stimulated by K+, Mn2+, Na2+ and triton X-100 and was strongly inhibited by Cu2+ and Fe2+ and the enzyme activity was relatively unaffected by Ca2+, CO2+, Mg2+ and EDTA. The Km and Vmax of the purified recombinant AnabfA activity towards ρNPA were 0.93 mM and 17.86 Όmol/ml/min, respectively.Key words: Aspergillus niger, α-L-arabinofuranosidase, expression, Pichia pastoris, characterisation

    Enumeration and identification of lactic microflora in Algerian goats’ milk

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    A total of 153 strains of lactic acid bacteria were isolated from Algerian goats’ milk. The strains were identified according to morphological, biochemical and physiological criteria, as well as the use of theAPI system and SDS-PAGE technique. Identification of the isolates revealed the presence of six genera: Enterococcus (41.82%), Lactobacillus (29.40), Lactococcus (19.60%), Leuconostoc (4.57%),Streptococcus thermophilus (3.26%) and Pediococcus (1.30%). The predominant strains belong to Enterococcus faecium (24 isolates), Enterococcus durans (22 isolates), Lactococcus lactis subsp. lactis(25 isolates), Lactobacillus rhamnosus (9 isolates) and Lactobacillus delbrueckii subsp. bulgaricus (7 isolates)

    Blood Pressure Home Monitoring in Hypertensive Patients Attending a Tertiary Health Facility in Amman, Jordan: Effect on Disease Control and Adherence Rate

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    Purpose: To investigate the effect of home monitoring of blood pressure (HMBP) on adherence to antihypertensive medications and control of the disease among patients attending a tertiary health facility in Amman, Jordan.Methods: This cross-sectional correlation study was conducted in 2012 at the cardiovascular clinics of Jordan University Hospital. A questionnaire investigating participants' disease level of control, disease duration, frequency of HMBP use, type of measuring devices used, and effect of HMBP on adherence to antihypertensive medications and life style changes was used for the study. To minimize any bias that may have resulted from the involvement of pharmacists in data collection, we designed a clear stringent protocol that was strictly followed by the researcher with all patients. In addition to that, the researcher clearly introduced herself as an independent figure that cannot make any decisions or changes.Results: Two hundred and five hypertensive patients who practice HMBP were recruited into this study. Sixty percent of the patients were aged 50 - 69 years. About 45 % of the patients were diagnosed with hypertension for > 10 years. Almost half of the patients have been using digital devices for blood pressure monitoring. The mean rate of HMBP was 15 times per month but almost 80 % of the patients did not document their blood pressure readings. Around 55 % of the patients referred high readings to a healthcare professional. Patients with higher levels of education as well as patients with lower BP readings showed significantly higher rates of adherence to their antihypertensive medications. Patients with high BP readings reported that they exercise more and reduced their daily salt intake.Conclusion: Overall, the patients showed high adherence rates to antihypertensive medications and adoption of better life style. However, increasing patient’s awareness in respect to documenting and communicating BP readings to their physicians is needed.Keywords: Adherence, Hypertension, Compliance, Blood pressure monitoring, Salt intake,Antihypertensive, Jorda
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