Rancang Bangun E-Library pada SMAN 1 Pagerbarang Tegal

Teknologi informasi merupakan salah satu hal penting pada era globalisasi saat ini. Perkembangan imu teknologi dan pengetahuan selalu memicu manusia untuk berkembang dan bersaing dalam pengusaan teknologi khususnya teknologi informasi dan komputer. Salah satunya internet, internet dapat memberikan kemudahan mencari informasi di segala aspek. Perkembangan ilmu teknologi berpengaruh pada dunia pendidikan. Banyak lembaga pendidikan yang memanfaatkan teknologi internet untuk memberikan informasi kepada masyarakat. Sistem infomasi perpustakaan merupakan suatu sistem yang memudahkan user untuk mencari informasi dengan menggunakan jaringan internet. Mempermudah dalam menyimpan data dan mengolah data dengan cepat dan tepat tanpa membutuhkan waktu lama dalam proses pengerjaannya. Melihat Kenyataan diatas, penulis membuat program sistem informasi e-library yang ditunjukan pada “SMA N 1 Pagerbarang, Tegal”. Bertujuan untuk mempermudah pelayanan perpustakaan dan mempermudah siswa mendapatkan buku referensi dan mata pelajaran. Siswa juga dipermudah untuk mencari informasi tentang artikel yang ada pada website e-library. Perancangan program e-library ini dibuat menggunakan Adobe Dreamweaver CS6, php dan mysql. Program ini dibuat untuk mempermudah siswa mendapatkan e-book tanpa harus datang ke perpustakaan. Dan mempermudah pegawai perpustakaan untuk mengolah data yang sudah ada. Program ini dibuat untuk memecahkan masalah yang ada seperti kendala waktu dan tempat. Dengan adanya program ini maka kegiatan akan lebih efektif dan efisien

The Autocrine Role of Proteoglycan-4 (PRG4) in Modulating Osteoarthritic Synoviocyte Proliferation and Expression of Matrix Degrading Enzymes

Background: Lubricin/proteoglycan 4 (PRG4) is a mucinous glycoprotein secreted by synovial fibroblasts and superficial zone chondrocytes. Recently, we showed that recombinant human PRG4 (rhPRG4) is a putative ligand for CD44 receptor. rhPRG4-CD44 interaction inhibits cytokine-induced rheumatoid arthritis synoviocyte proliferation. The objective of this study is to decipher the autocrine function of PRG4 in regulating osteoarthritic synoviocyte proliferation and expression of catabolic and pro-inflammatory mediators under basal and interleukin-1 beta (IL-1β)- stimulated conditions. Methods: Cytosolic and nuclear levels of nuclear factor kappa B (NFκB) p50 and p65 subunits in Prg4+/+ and Prg4-/- synoviocytes were studied using western blot. Nuclear translocation of p50 and p65 proteins in osteoarthritis (OA) fibroblast-like synoviocytes (FLS) in response to IL-1β stimulation in the absence or presence of rhPRG4 was studied using DNA binding assays. OA synoviocyte (5000 cells per well) proliferation following IL-1β (20 ng/ml) treatment in the absence or presence of rhPRG4 (50–200 μg/ml) over 48 hours was determined using a colorimetric assay. Gene expression of matrix metalloproteinases (MMPs), tissue inhibitor of metallproteinases-1 (TIMP-1), TIMP-2, IL-1β, IL-6, IL- 8, TNF-α, cycloxygenae-2 (COX2) and PRG4 in unstimulated and IL-1β (1 ng/ml)-stimulated OA synoviocytes, in the presence or absence of rhPRG4 (100 and 200 μg/ml), was studied following incubation for 24 hours. Results: Prg4-/- synoviocytes contained higher nuclear p50 and p65 levels compared to Prg4+/+ synoviocytes (p \u3c 0. 05). rhPRG4 (100 μg/ml) reduced p50 and p65 nuclear levels in Prg4+/+ and Prg4-/- synoviocytes (p \u3c 0.001). Similarly, rhPRG4 (200 μg/ml) inhibited NFκB translocation and cell proliferation in OA synoviocytes in a CD44-dependent manner (p \u3c 0.001) via inhibition of IκBα phosphorylation. IL-1β reduced PRG4 expression in OA synoviocytes and rhPRG4 (100 μg/ml) treatment reversed this effect (p \u3c 0.001). rhPRG4 (200 μg/ml) reduced basal gene expression of MMP-1, MMP-3, MMP-13, IL-6, IL-8, and PRG4 in OA synoviocytes, while increasing TIMP-2 and cycloxygenase-2 (COX2) expression (p \u3c 0.001). rhPRG4 (200 μg/ml) reduced IL-1β induction of MMP-1, MMP-3, MMP-9, MMP-13, IL-6, IL-8, and COX2 expression in a CD44-dependent manner (p \u3c 0.001). Conclusion: PRG4 plays an important anti-inflammatory role in regulating OA synoviocyte proliferation and reduces basal and IL-1β-stimulated expression of catabolic mediators. Exogenous rhPRG4 autoregulates native PRG4 expression in OA synoviocytes

The Level of Inclusion of Transitional Services Competencies in Special Education Programs in Saudi Universities

The study aimed to identify the level of inclusion of transitional services competencies in special education programs in Saudi universities from the point of view of faculty members. In addition to studying the differences between the responses of faculty members according to the variables: the date of establishing the program, the number of courses related to transitional services, and the availability of specialists in this field. The sample consisted of 164 faculty members in special education programs in 22 Saudi universities. Results indicated that the inclusion of transitional services competencies in the special education programs in Saudi universities came to a moderate degree. Moreover, these competencies are more included in older established special education programs, programs with more courses related to transitional services, and programs that have specialists in this field. Finally, the results included some recommendations to improve the inclusion of competencies in special education programs in those universities. Keywords: Competencies of pre-service teachers, Transitional Services, Special Education Programs

Proteoglycan-4 Regulates Fibroblast to Myofibroblast Transition and Expression of Fibrotic Genes in the Synovium

Background: Synovial tissue fibrosis is common in advanced OA with features including the presence of stress fiber-positive myofibroblasts and deposition of cross-linked collagen type-I. Proteoglycan-4 (PRG4) is a mucinous glycoprotein secreted by synovial fibroblasts and is a major component of synovial fluid. PRG4 is a ligand of the CD44 receptor. Our objective was to examine the role of PRG4-CD44 interaction in regulating synovial tissue fibrosis in vitro and in vivo. Methods: OA synoviocytes were treated with TGF-β ± PRG4 for 24h and α-SMA content was determined using immunofluorescence. Rhodamine-labeled rhPRG4 was incubated with OA synoviocytes ± anti-CD44 or isotype control antibodies and cellular uptake of rhPRG4 was determined following a 30-min incubation and α-SMA expression following a 24-h incubation. HEK-TGF-β cells were treated with TGF-β ± rhPRG4 and Smad3 phosphorylation was determined using immunofluorescence and TGF-β/Smad pathway activation was determined colorimetrically. We probed for stress fibers and focal adhesions (FAs) in TGF-β-treated murine fibroblasts and fibroblast migration was quantified ± rhPRG4. Synovial expression of fibrotic markers: α-SMA, collagen type-I, and PLOD2 in Prg4 gene-trap (Prg4GT) and recombined Prg4GTR animals were studied at 2 and 9 months of age. Synovial expression of α-SMA and PLOD2 was determined in 2-month-old Prg4GT/GT&Cd44−/− and Prg4GTR/GTR&Cd44−/− animals. Results: PRG4 reduced α-SMA content in OA synoviocytes (p \u3c 0.001). rhPRG4 was internalized by OA synoviocytes via CD44 and CD44 neutralization attenuated rhPRG4’s antifibrotic effect (p \u3c 0.05). rhPRG4 reduced pSmad3 signal in HEKTGF- β cells (p \u3c 0.001) and TGF-β/Smad pathway activation (p \u3c 0.001). rhPRG4 reduced the number of stress fiberpositive myofibroblasts, FAs mean size, and cell migration in TGF-β-treated NIH3T3 fibroblasts (p \u3c 0.05). rhPRG4 inhibited fibroblast migration in a macrophage and fibroblast co-culture model without altering active or total TGF-β levels. Synovial tissues of 9-month-old Prg4GT/GT animals had higher α-SMA, collagen type-I, and PLOD2 (p \u3c 0.001) content and Prg4 re-expression reduced these markers (p \u3c 0.01). Prg4 re-expression also reduced α-SMA and PLOD2 staining in CD44-deficient mice. Conclusion: PRG4 is an endogenous antifibrotic modulator in the joint and its effect on myofibroblast formation is partially mediated by CD44, but CD44 is not required to demonstrate an antifibrotic effect in vivo

Using VARK to assess Saudi nursing students' learning style preferences: Do they differ from other health professionals?

أهداف البحث: تهدف هذه الدراسة لمعرفة أسلوب التعلم المفضل لطالبات التمريض السعوديات، الأمر الذي يؤدي إلى فهم محتوى المنهج وبالتالي تحسين رعاية المرضى. طرق البحث: وزعت استبانة مسحية مقطعية على ١٢٥ طالبة تمريض سعودية تطوعت بالاشتراك في هذا البحث. النتائج: فضل أكثر من ٨٠.٥٪ من المشاركات التعلم الحركي. وفضلت بقوة ٣٨.٢٪ منهن التعلم الحركي، في حين هيمن تفضيل ١٠.٦ ٪٬ و ٤.٩٪ و ٢.٤٪ للتعلم السماعي، والقراءة/الكتابة، والتعلم البصري، على التوالي. ولم تكن أساليب التعلم لطالبات التمريض السعوديات مختلفه كثيرا في تفضيلهن التعلم الحركي عن مجموعة طالبات التمريض الاستراليات، ولكن كانت مختلفة إلى حد كبير في تفضيل التعلم الحركي عن طالبات الطب السعوديات. وحصل أسلوب التعلم الحركي على أعلى رتبة تفضيل بين جميع مجموعات طالبات التمريض. الاستنتاجات: كانت أساليب التعلم المذكورة لطالبات التمريض السعوديات أكثر مماثلة لمجموعات أخرى من الممرضات عن طالبات التخصصات الصحية السعوديات في مجالات رئيسة لتفضيل أساليب التعلم