NTPase and 5′-RNA Triphosphatase Activities of Chikungunya Virus nsP2 Protein

Chikungunya virus (CHIKV) is an insect borne virus (genus: Alphavirus) which causes acute febrile illness in humans followed by a prolonged arthralgic disease that affects the joints of the extremities. Re-emergence of the virus in the form of outbreaks in last 6–7 years has posed a serious public health problem. CHIKV has a positive sense single stranded RNA genome of about 12,000 nt. Open reading frame 1 of the viral genome encodes a polyprotein precursor, nsP1234, which is processed further into different non structural proteins (nsP1, nsP2, nsP3 and nsP4). Sequence based analyses have shown helicase domain at the N-terminus and protease domain at C-terminus of nsP2. A detailed biochemical analysis of NTPase/RNA helicase and 5′-RNA phosphatase activities of recombinant CHIKV-nsP2T protein (containing conserved NTPase/helicase motifs in the N-terminus and partial papain like protease domain at the C-terminus) was carried out. The protein could hydrolyze all NTPs except dTTP and showed better efficiency for ATP, dATP, GTP and dGTP hydrolysis. ATP was the most preferred substrate by the enzyme. CHIKV-nsP2T also showed 5′-triphosphatase (RTPase) activity that specifically removes the γ-phosphate from the 5′ end of RNA. Both NTPase and RTPase activities of the protein were completely dependent on Mg2+ ions. RTPase activity was inhibited by ATP showing sharing of the binding motif by NTP and RNA. Both enzymatic activities were drastically reduced by mutations in the NTP binding motif (GKT) and co-factor, Mg2+ ion binding motif (DEXX) suggesting that they have a common catalytic site

“In Vitro study of Antibacterial properties of GokshurKwatha in Urinary Tract Infection by Culture and Sensitivity Method.”

The study was planned to evaluate the anti bacterial property of GokshurKwatha onUrinary Tract Infection by culture & sensitivity method for the study,30 patients were selected & urine sample used for culture by stricking method & colony characters are observed & gram staining done. Then specially made Gokshurkwath discs were used for sensitivity in MH agar. Zone of inhibition is the criteria of assessment of sensitivity. Whole study was in vitro. The bacterias during study are Escherichia Colii, KlebsiellaPnuemonii,Pseudomonas. It observed that Gokshurkwath shows significant activity against above organism

Overview of Auditing Cloud Consistency

Cloud storage services have become very popular due to their infinite advantages. To provide always-on access, a cloud service provider (CSP) maintains multiple copies for each piece of data on geographically distributed servers. A major disadvantage of using this technique in clouds is that it is very expensive to achieve strong consistency on a worldwide scale. In this system, a novel consistency as a service (CaaS) model is presented, which involves a large data cloud and many small audit clouds. In the CaaS model we are presented in our system, a data cloud is maintained by a CSP. A group of users that participate an audit cloud can verify whether the data cloud provides the promised level of consistency or not. The system proposes a two level auditing architecture, which need a loosely synchronize clock in the audit cloud. Then design algorithms to measure the severity of violations with two metrics: the commonality of violations, and the oldness value of read. Finally, heuristic auditing strategy (HAS) is devised to find out as many violations as possible. Many experiments were performed using a combination of simulations and a real cloud deployment to validate HAS. DOI: 10.17762/ijritcc2321-8169.15011

Effect of organic farming practices on soil and performance of soybean (Glycine max) under semi-arid tropical conditions in Central India

A field experiment was conducted to evaluate the influence of organic farming practices on soil health and crop performance of Soybean (Glycine max).The crop cultivar JS-335 of soybean was grown with 30:26.2:16.6 kg ha-1 (NPK) recommended dose of fertilizers under three management practices viz., organic, chemical and integrated (50:50) in randomized block design, replicated three times. Soil organic carbon, available N, P and K, microbial enzymatic activities, total biomass, seed yield and harvest index (HI) were analysed during the study. It was observed that soil organic carbon (11.3 g kg-1), available N (125 mg kg-1), P (49.7 mg kg-1) and soil enzyme activities viz., dehydrogenase (DHA) (98.20 µ grams TPF/g soil/24 h) and alkaline phosphatase (178.2 µ grams p-nitro phenol/g soil/h) were found significantly higher in the plot managed organically while available K (320.1 mg kg-1) was not significant with respect to chemical and integrated practices. The total biomass (1927 kg ha-1) and seed yield (601 kg ha-1) of soybean was found highest in organic farming practices followed by integrated and chemical practices. Very poor microbial activities were observed in chemically managed plots. Thus, the study demonstrated that the organic farming practice improved soil health and performance of soybean crop

The effect of modafinil on the rat dopamine transporter and dopamine receptors D1–D3 paralleling cognitive enhancement in the radial arm maze

A series of drugs have been reported to increase memory performance modulating the dopaminergic system and herein modafinil was tested for its working memory (WM) enhancing properties. Reuptake inhibition of dopamine, serotonin (SERT) and norepinephrine (NET) by modafinil was tested. Sixty male Sprague–Dawley rats were divided into six groups (modafinil-treated 1–5–10 mg/kg body weight, trained and untrained and vehicle treated trained and untrained rats; daily injected intraperitoneally for a period of 10 days) and tested in a radial arm maze (RAM), a paradigm for testing spatial WM. Hippocampi were taken 6 h following the last day of training and complexes containing the unphosphorylated or phosphorylated dopamine transporter (DAT-CC and pDAT-CC) and complexes containing the D1–3 dopamine receptor subunits (D1–D3-CC) were determined. Modafinil was binding to the DAT but insignificantly to SERT or NET and dopamine reuptake was blocked specifically (IC50 = 11.11 μM; SERT 1547 μM; NET 182 μM). From day 8 (day 9 for 1 mg/kg body weight) modafinil was decreasing WM errors (WMEs) in the RAM significantly and remarkably at all doses tested as compared to the vehicle controls. WMEs were linked to the D2R-CC and the pDAT-CC. pDAT and D1–D3-CC levels were modulated significantly and modafinil was shown to enhance spatial WM in the rat in a well-documented paradigm at all the three doses and dopamine reuptake inhibition with subsequent modulation of D1–3-CC is proposed as a possible mechanism of action. © 2015 Karabacak, Sase, Aher, Sase, Saroja, Cicvaric, Höger, Berger, Bakulev, Sitte, Leban, Monje and Lubec

Loss-of-function of triacylglycerol lipases are associated with low flour rancidity in pearl millet [Pennisetum glaucum (L.) R. Br.]

Pearl millet is an important cereal crop of semi-arid regions since it is highly nutritious and climate resilient. However, pearl millet is underutilized commercially due to the rapid onset of hydrolytic rancidity of seed lipids post-milling. We investigated the underlying biochemical and molecular mechanisms of rancidity development in the flour from contrasting inbred lines under accelerated aging conditions. The breakdown of storage lipids (triacylglycerols; TAG) was accompanied by free fatty acid accumulation over the time course for all lines. The high rancidity lines had the highest amount of FFA by day 21, suggesting that TAG lipases may be the cause of rancidity. Additionally, the high rancidity lines manifested substantial amounts of volatile aldehyde compounds, which are characteristic products of lipid oxidation. Lipases with expression in seed post-milling were sequenced from low and high rancidity lines. Polymorphisms were identified in two TAG lipase genes (PgTAGLip1 and PgTAGLip2) from the low rancidity line. Expression in a yeast model system confirmed these mutants were non-functional. We provide a direct mechanism to alleviate rancidity in pearl millet flour by identifying mutations in key TAG lipase genes that are associated with low rancidity. These genetic variations can be exploited through molecular breeding or precision genome technologies to develop elite pearl millet cultivars with improved flour shelf life

Track D Social Science, Human Rights and Political Science

Peer Reviewedhttps://deepblue.lib.umich.edu/bitstream/2027.42/138414/1/jia218442.pd

A 30-Year Worldwide Research Productivity of Scientific Publication in Full-Endoscopic Decompression Spine Surgery: Quantitative and Qualitative Analysis

Objective The ever-growing number of articles related to full-endoscopic spine surgery published in the last few decades presents a challenge which is perplexing and time-consuming in identifying the current research status. The study aims to identify and analyze the most cited works related to full-endoscopic decompression spine surgery, compare the articles published by different publishers and area, and show the current publication status of full-endoscopic research. Methods Using Bibliometrix, CiteSpace, and VOSviewer, we analyzed the bibliometric data selected from the Web of Science database between 1992 and 2022. Spine has the highest H-index with the most-cited journal in the field of full-endoscopic decompression spine surgery. China ranked as the most productive country, whereas the most cited with high H-index papers came from South Korea. For the author analysis, Yeung AT, Ruetten S, Hoogland T, Ahn Y, Choi G, and Mayer HM were the most impactful authors in the global and local citations. The most productive organization is Wooridul Spine Hospital. Conclusion The bibliometric study showed a growing trend of research on full-endoscopic decompression spine surgery over the past 30 years. It has demonstrated that there is a significant increase in the number of authors, institutions, and internationally collaborated countries. However, the quality of studies is still low, and the lack of high-quality clinical evidence and the trend of general journal submissions has somewhat affected the quality of endoscopy journals in recent years