Single-stage laparoscopic transanal pull-through modified Swenson procedure without leaving a muscular cuff for short- and long-type Hirschsprung disease: a comparative study

Purpose: The Soave procedure (SO) is performed most commonly for Hirschsprung disease. SO reduces the risk of injury to the pelvic structures; however, a residual aganglionic muscle cuff could interfere with bowel movement and lead to obstructive enterocolitis. The Swenson procedure is considered ideal in terms of peristalsis. Currently, laparoscopic surgery provides better visualization and facilitates precise dissection, possibly leading to feasible performance of the laparoscopic modified Swenson procedure (SW). We present our operative technique and the efficacy of the SW compared with that of SO. Methods: We retrospectively reviewed the records of 16 and 27 patients who underwent SW and SO, respectively, between 2012 and 2017. Results: Operative time, blood loss, length of stay, and frequency of bowel movements showed no significant difference between the two groups. In the SW group, temporary dysuria occurred in one patient, postoperative enterocolitis in two, wound infection in one, and severe perianal excoriation in four, whereas in the SO group, obstructive symptoms occurred in three patients, small-bowel obstruction in one, and severe perianal excoriation in three. The complications and outcomes were comparable between both groups. Conclusion: Laparoscopic SW was safe and feasible for the short-term follow-up outcomes.ファイル公開：2019/10/01journal articl

91 出血及び延髄圧迫症状を呈した血栓化椎骨動脈瘤の2症例(北日本脳神経外科連合会第27回学術集会)

departmental bulletin pape

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-0

� or left untreated (-), and transduced 24 h later with VSV-pseudotyped GFP-expressing N-MLV (N), HIV-1 (HIV) or NB-MLV (NB) at low (MOI = 0.5) or high (MOI = 5) multiplicity of infection (as determined on MDTF cells). The percentage of transduced (GFP-positive) cells was determined by FACS 48 h post-transduction.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-4

-MLV or NB-MLV at a MOI of 5. CsA (5 μM) was added 2 h before transduction. Percentage of transduced cells was determined by FACS 48 h post-transduction. Fold restriction represents the ratio of untreated to IFN-β-treated cells to be transduced by the GFP-encoding retroviral vectors. Results are representative of two independent experiments with comparable results. A significant enhancement of retroviral restriction is defined as a ratio > 2. . OMK cells were stimulated with IFN-β and transduced 24 h later with VSV-pseudotyped GFP-expressing HIV-1, SIVmac or HIV-1 SCA vectors at a MOI of 1. The percentage of transduced cells was determined by FACS 48 h post-transduction. Fold restriction represents the ratio of untreated/IFN-β-treated cells to be transduced. As in panel A, a significant enhancement of retroviral restriction is defined as a ratio > 2.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-2

1, HA2 or A3), as indicated, and stimulated 24 h later with 1000 U/ml of IFN-β for 8 h. Total RNA was extracted and the levels of TRIM5α mRNA were determined by quantitative RT-PCR and normalized to GAPDH. The mean ± SD of duplicates is shown. . HeLa or Vero cells were transfected with siRNA targeting luciferase (Luc), TRIM5α(H1 or HA2) or TRIM5α(HA2 or A3), as indicated. The next day, cells were stimulated with 1000 U/ml of IFN-β for 8 h and challenged with a GFP-expressing N-MLV vector. The percentage of GFP-positive cells was determined by FACS 48 h post-transduction. Data are from a typical experiment representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-3

as indicated, and stimulated 24 h later with 1000 U/ml of IFN-β for 8 h. Total RNA was extracted and the levels of TRIMCyp mRNA were determined by quantitative RT-PCR and normalized to GAPDH. The mean ± SD of duplicates is shown. . OMK cells were transfected with anti-Luc (diamonds) or anti-TRIMCyp (triangles) siRNA and transduced 48 h later with increasing doses of HIV-1. The percentage of GFP-positive cells was determined by FACS 48 h post-transduction. . Same experiment as in panel B, except that cells were challenged with HIV-1, N-MLV, B-MLV or NB-MLV (at a MOI of 5), following siRNA and IFN-β treatments. The percentage of GFP-positive cells was determined by FACS 48 h post-transduction. Data are from a typical experiment representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-1

GFP-expressing N-MLV (N), HIV-1 (HIV), NB-MLV (NB), B-MLV (B) or SIVmac (SIV) at low (MOI = 0.5) or high (MOI = 5) multiplicity of infection. The percentage of transduced cells was determined by FACS 48 h post-transduction.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities-5

D GFP-expressing SIVmac (SIV), HIV-1 (HIV), N-, B-, or NB-tropic MLV vectors. The same experiment was performed in parallel in the presence of AZT at 5 μM during infections. Total DNA was extracted 6 h post-transduction and the amount of reverse transcripts was estimated by PCR using GFP primers. A PCR on CypA was also performed as a control.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p

Normalizing β-arrestin-dependent signaling of CXCR4¹⁰¹³ partially restores the productive HPV life cycle.

(A) Western blots (left) and densitometric analyses (right) showing relative levels of HPV18-E2 protein in HPV18-positive CXCR4wt, CXCR41013, and CXCR41013&ΔSHSK raft cultures. E2 protein levels were normalized to GAPDH protein levels and arbitrarily set at 1 for CXCR4wt rafts (staining controls are shown in S5 Fig). Values are means ± SEM. **p 1013&ΔSHSK raft sections stained for HPV18-E4 (B), HPV18-L1 (C), and Ki-67 (D) expression by immunohistochemical staining. Images are representative of three independent experiments. Scale bars = 100 μm, inset scale bar = 10 μm.</p

Expression of CXCR4¹⁰¹³ does not modify the architecture of raft cultures or the differentiation program of keratinocytes.

(A) Representative HPV18-positive CXCR4wt and CXCR41013 raft culture sections stained with hematoxylin, eosin, and safran (HES) (A) or stained for keratin 10 (B) or filaggrin (C). Images are representative of three independent experiments. Scale bars = 100 μm.</p