1, HA2 or A3), as indicated, and stimulated 24 h later with 1000 U/ml of IFN-β for 8 h. Total RNA was extracted and the levels of TRIM5α mRNA were determined by quantitative RT-PCR and normalized to GAPDH. The mean ± SD of duplicates is shown. . HeLa or Vero cells were transfected with siRNA targeting luciferase (Luc), TRIM5α(H1 or HA2) or TRIM5α(HA2 or A3), as indicated. The next day, cells were stimulated with 1000 U/ml of IFN-β for 8 h and challenged with a GFP-expressing N-MLV vector. The percentage of GFP-positive cells was determined by FACS 48 h post-transduction. Data are from a typical experiment representative of three independent experiments.<p><b>Copyright information:</b></p><p>Taken from "Implication of TRIMalpha and TRIMCyp in interferon-induced anti-retroviral restriction activities"</p><p>http://www.retrovirology.com/content/5/1/59</p><p>Retrovirology 2008;5():59-59.</p><p>Published online 9 Jul 2008</p><p>PMCID:PMC2483995.</p><p></p
