Saccharification and Ethanol Production from Chlorella sp. Through High Speed Extrusion Pretreatment

Among various pretreatment processes for bioethanol production, extrusion pretreatment, one of cheap and simple process was investigated to efficiently produce fermentable sugars from micro alga, Chlorella sp. The biomass was pretreated in a single screw extruder at five different barrel temperatures of 45, 50, 55, 60 and 65∘C , respectively with five screw rotation speed of 10, 50, 100, 150 and 200 rpm. The pretreated biomass was reacted with two different hydrolyzing enzymes of cellulase and amyloglucosidase since the biomass contained different types of carbohydrates, compared to cellulose of agricultural by-products such wheat and corn stovers, etc. In general, higher glucose conversion yield was obtained as 13.24 (%, w/w) at 55∘C of barrel temperature and 100 rpm of screw speed conditions. In treating 5 FPU/glucan of cellulase and 150 Unit/mL of amyloglucosidase, ca. 64% of cellulose and 40% of polysaccharides in the micro alga were converted into glucose, which was higher yields than those from other reported data without applying an extrusion process. 84% of the fermentable sugars obtained from the hyrolyzing processes were fermented into ethanol in considering 50% of theoretical maximum fermentation yield of the yeast. These results implied that high speed extrusion could be suitable as a pretreatment process for the production of bioethanol from Chlorella sp.22Nkc

Method for Saccharification of Marine Algae using Extrusion Process with High Pressure

본 발명은 고압 압출 공정으로 압출된 해조류를 전처리하여 당화시키는 방법에 관한 것으로서, 보다 구체 적으로 고압 압출 공정과, a) 마이크로웨이브 공정, b) 초음파 공정, c) 초고압 공정 및 d) 균질화 공정 중에서 선택된 공정을 복합 진행하여 해조류를 전처리하고, 당화 효소를 처리함으로써, 산 또는 알칼리 물 질을 사용하지 아니하고, 친환경적으로 해조류를 당화시키는 방법에 관한 것이다. 본 발명에 의해 해조류를 구성하는 다당류인 셀룰로오스, 헤미셀룰로오스, 전분 및 복합 다당류 등의 난분 해성 고분자를 물을 이용한 친환경적인 전처리 공정에 의하여 높은 당화 효율로 가수분해 할 수 있다