Reseaching about the Construction of Social Services Ability of Universities in China

在高等教育发展的早期，高校的主要职能是传授高深知识，探究高深学问，游离于社会之外，因此被喻为“象牙塔”。随着时代的发展和社会的进步，社会对高校不断提出新的要求，高校的职能也在随之不断地拓展和丰富，从最初单一的人才培养职能发展到人才培养、科学研究和社会服务三大职能。其中，高校社会服务职能是最晚产生的，理论界对这一职能的产生和发展历程观点比较统一，但对其现状和存在的问题则众说纷纭。 本文希望在借鉴以往研究成果的基础上，能够有所创新和发展。同时，还进入实践领域，期望在实践层面为国内高校的社会服务能力建设提出方案，为其科学决策、实现跨越式发展做出贡献。全文主要从以下几个方面作了探索： 第一，从理论...In the early days, main functions of universities were teaching profound knowledge and inquiring profound scholarship. And because of the isolation out of society, the universities were compared to “ivory tower”. As the times and social progressing, the requirement of society was changing. Then, the functions of university were constantly expanding and rich. And now, it not only includes talent tr...学位：管理学硕士院系专业：公共事务学院公共管理系_行政管理学号：1392007115067

Interleave-division-multiple space-time system using LDPC codes

交织分复用空时码(IdM-ST)技术是第四代移动通信的关键技术之一,设计了一种基于ldPC编码的交织分复用空时码系统,并将它与传统的基于卷积码编码的IdM-ST系统在准静态的瑞利衰落信道下进行了比较。仿真结果表明,在不同帧长,不同天线数情况下,基于ldPC编码的IdM-ST系统和基于卷积码编码系统各有其优势。整个系统具有接收简单,复杂度低等优点。在实际应用中,适合于高速的数据传输业务。IDM-ST is one of key technologies in the fourth generation of mobile telecommunication.A LDPC coded IDM-ST system was designed and it has been compared with a convolutional coded IDM-ST system over quasi-static Rayleigh fading channels.Simulation results show that the proposed system and the convolutional coded system have their own predominances with different frame length and different antenna numbers.The proposed system has simple receiver and low complexity.In practical application,it is very suitable for high-speed transmission operation.教育部新世纪优秀人才支持计划项目(NCET-04-0601);重庆市科委自然科学基金项目(CSTC;2007BB2387

Study on preparation and in vitro release behavior of hydroxycamptothecin-loaded PLA microspheres

目的:研究载羟基喜树碱的聚乳酸微球的制备方法并考察其体外释药性质。方法:以PlA为成膜材料,采用改良乳化-溶剂挥发法,制备载羟基喜树碱的聚乳酸微球并优化制备工艺;对载药微球进行表征;超声介导下进行载药微球的体外释药试验。结果:微球粒径在1~7μM,大小均一;羟基喜树碱浓度在10Mg.Ml-1下,载药微球包封率为62.2%,载药量为1.69%;药物体外释药符合HIguCHI方程。结论:采用乳化-溶剂挥发法,以PlA为成膜材料可制得具有较高包封率的羟基喜树碱微球,有望实现降低羟基喜树碱给药量、减少不良反应,提高靶向性的目标。Objective:To prepare hydroxycamptothecin(HCPT)-loaded PLA microspheres and study the release of HCPT in vitro.Methods:To optimize the preparation process,the HCPT-loaded PLA microspheres were prepared by using an improved solvent evaporation method;drug-loaded microspheres was characterized;in vitro drug release experiments were carried out under ultrasound.Results:The sizes of drug loaded PLA microspheres were homogeneous and between 1--7 μm;the concentration of HCPT was 10 mg·mL-1,the drug encapsulation efficiency was 62.2%,and the drug-loading amounts was 1.69%;the release in vitro complied with Higuchi equation.Conclusion:The improved solvent evaporation method with PLA as the film-forming material can increase the encapsulation efficiency of HCPT-loaded microspheres,thus the reduction of dosage and improvement of toxic effects of HCPT would be expected.Targeting effect may be improved.福建省卫生厅青年科研项目(2009-2-79

Pharmacokinetics of Dual Conjugated Chitosan-mitomycin C Nanoparticles in Rats

目的研究双修饰壳聚糖载丝裂霉素C纳米粒在大鼠体内的药动学特征。方法 2组大鼠分别静脉注射4 Mg·kg·1丝裂霉素C纳米粒和丝裂霉素C注射剂后,采用高效液相色谱-串联质谱法(HPlC-MS)测定给药后不同时间点血浆中丝裂霉素C的浓度,计算主要药动学参数。结果丝裂霉素C的线性范围20~1 000μg·l·1,最低定量限为20μg·l·1,提取回收率均>95%,日内、日间精密度rSd均<15%。双修饰壳聚糖载丝裂霉素C纳米粒和丝裂霉素C注射剂T1/2分别为(2.64±0.11)H、(0.49±0.049)H;AuC0-∞分别为(2.01±0.11)Mg·H·1·l·1、(0.93±0.075)Mg·H·1·l·1;Vz分别为(1.52±0.18)l、(0.63±0.065)l;Cl分别为(6.95±0.70)Ml·MIn·1、(15.47±1.89)Ml·MIn·1,2者均有显著性差异。结论该方法灵敏、准确、专一,适用于丝裂霉素C的药动学研究。与丝裂霉素C注射剂相比,双修饰壳聚糖载丝裂霉素C纳米粒具有缓释和长循环的作用。OBJECTIVE To study the pharmacokinetics of dual conjugated chitosan-mitomycin C nanoparticles(CS-MMC-NPs) in rats.METHODS The two groups of rats were injected with CS-MMC-NPs and MMC injection at the dose of 4 mg·kg·1.The concentrations of MMC in plasma at different time were determined by HPLC-MS and the main pharmacokinetic parameters were calculated.RESULTS The calibration curves were linear over the range of 20·1 000 μg·L·1.The limit of quantitation was 20 μg·L·1.The within day and day to day relative standard deviation(RSD) was <15%.The main pharmacokinetic parameters of CS-MMC-NPs and MMC injection were as follows: t1/2 were(2.64±0.11)h and(0.49±0.049)h, AUC0- ∞ were(2.01±0.11)mg·h·1·L·1 and(0.93±0.075)mg·h·1·L·1, Vz were(1.52±0.18)L and(0.63±0.065)L, CL were(6.95±0.70)mL·min·1 and(15.47±1.89)mL·min·1.The differences of parameters were significant between two preparations.CONCLUSION The method is sensitive, accurate, specific for the pharmacokinetic study of CS-MMC-NPs.Compared with MMC injection, CS-MMC-NPs has a controlled releasing rate, a high level of blood concentrations and a long blood circulation time, which benefits the control of acute toxicity of MMC for the rats.厦门市科学技术计划项目资助项目(3502Z20114007

Preparation and Characterization of Folic Acid and PEG Conjugated Chitosan Nanoparticles

目的利用离子交联和化学交联相结合的方法制备壳聚糖纳米粒子(nPS),并对nPS分别进行了叶酸(fA)和聚乙二醇(PEg)的修饰。方法通过红外光谱进行结构验证;用扫描电镜和粒度分析仪对粒子的微观形态、粒径、电位等进行了表征;通过与HElA细胞摄取实验对其靶向作用进行验证。结果离子交联和化学交联相结合的方法制备壳聚糖纳米粒子粒径在200 nM左右并且粒径分布窄,修饰后的nPS(fA-nPS、PEg-nPS及fA+PEg-nPS)粒径不受功能基团修饰的影响。激光共聚焦试验证明fA-nPS及fA+PEg-nPS能显著提高细胞对粒子的摄取,而PEg-nPS则明显降低其对粒子的摄取。结论 fA+PEg-nPS有望成为一种新型的药物载体,用于抗癌药物对癌细胞的主动靶向。OBJECTIVE An ionic gelation combined with chemical crosslinking method was developed to prepare chitosan nanoparticles,followed by conjugation with folate(FA) and polyethylene glycol(PEG).METHODS The structures were verified by infrared spectroscopy.The morphology,diameter and Zeta electric potential of the nanoparticles were assayed by environmental scanning electron microscope and scattering particle analyzer.The specificity of the FA+PEG-NPs targeting cancer cells was demonstrated by human adenocarcinoma Hela cells.RESULTS The chitosan NPs presented a narrow size distribution with an average diameter about 200 nm regardless of the type of functional group.Laser confocal scanning imaging proved that both FA+PEG-NPs and FA-NPs could greatly enhance uptake by Hela cells.However,the PEG-NPs showed contrary results.CONCLUSION FA+PEG-NPs can be applied as a new vehicle to actively deliver anticancer drugs to tumor cells.厦门市科学技术计划资助项目(3502Z20114007

EB病毒潜伏膜蛋白1介导c-Jun/JunB活性异源二聚体形成

EB病毒编码的潜伏膜蛋白1可以活化AP-1转录因子, 其中c-Jun和JunB的相互关系和复杂作用一直是人们关注的焦点. 以Tet-on-LMP1 HNE2鼻咽癌细胞系为动态研究模型, 主要应用c-Jun/Jun B双染色间接免疫荧光法联合激光共聚焦荧光显微镜技术、Western blot方法、免疫共沉淀-Western blot方法以及Super-EMSA方法, 同时, 结合信号转导通路研究中的阻断策略, 研究证实EB病毒编码的潜伏膜蛋白1介导c-Jun/Jun B异源二聚体形成, 而且该二聚体具有与DNA结合活性. 该研究为LMP1调控下, AP1二聚体家族成员在不同时空信号传导通路中的动态组合和作用模式提供了新的机制.国家重点基础研究发展规划(973)项目(批准号:G1998051201);国家自然科学基金项目(批准号: 30300403,30100005,30000087);国家杰出青 年科学基金项目(批准号: 39525022)资