CFTR对前脂肪细胞增殖和分化的影响

【目的】探讨囊性纤维化跨膜转导调节因子（CFTR）对内脏脂肪组织前脂肪细胞增殖和分化的调控作用。【方法】分别采用CFTR基因敲除小鼠（CFTRKO）4、8周龄及同周龄野生型对照鼠，分离内脏脂肪组织前脂肪细胞，采用实时荧光定量PCR（RT-qPCR）技术检测增殖分化转录因子表达变化。采用地塞米松-异丁基甲基黄嘌呤-胰岛素（DMI）诱导3T3-L1细胞建立前脂肪细胞分化模型，采用免疫印迹（Western blot）技术检测CFTR及分化因子蛋白表达变化；并进一步转染CFTR沉默及过表达病毒，采用免疫印迹，实时荧光定量PCR技术，MTT检测和油红O染色，观察基因干预CFTR后对DMI诱导3T3-L1细胞增殖及分化的影响。采用高脂饮食肥胖小鼠模型，荧光定量PCR技术检测内脏前脂肪细胞增殖分化转录因子和CFTR基因水平表达。【结果】CFTR基因敲除鼠内脏前脂肪细胞指标Pref-1，增殖因子CyclinD1，分化转录因子PPARγ，C/EBPα表达明显降低（P<0.05）。DMI诱导3T3-L1细胞分化早期，CFTR的表达增加（P<0.05）；转染CFTR沉默病毒后，抑制DMI诱导的3T3-L1细胞增殖（C/EBPβ，C/EBPδ，CREB，KLF4）及末端分化指标（SREBP-1，PPARγ，C/EBPα）表达，而转染CFTR过表达病毒后，促进DMI诱导的3T3-L1细胞增殖及末端分化。高脂饮食喂养小鼠2，4周，高脂饮食组内脏前脂肪细胞增殖分化转录因子（PPARγ，C/EBPα，C/EBPβ，C/EBPδ，SREBP-1，FABP4）表达升高，CFTR基因表达升高（P<0.05）。【结论】CFTR可能通过调控前脂肪细胞增殖及分化影响脂肪组织功能

新生儿原发性先天性青光眼手术疗效分析

【目的】探讨出生即发病的原发性先天性青光眼（NPCG）患儿早期行滤过手术的疗效。【方法】收集3岁以内接受小梁切开术或小梁切开联合小梁切除术NPCG患儿39例70眼，比较术前和术后眼压、眼前段情况，角膜横径，杯/盘比，并记录随访视力。采用Kaplan-Meier生存分析法分析手术成功率，Log-Rank检验法比较出生1月内手术（22眼）与1月后手术（48眼）的手术成功率。【结果】所有患儿术后眼压（mmHg；16.9±5.2）比术前（28.7±5.8）明显降低（P<0.001），术后角膜变透明或混浊减轻、角膜横径无变化、C/D值（杯盘比）减小，Kaplan-Meier生存分析法得出手术成功率在术后第1、2、3、6、9年分别为94.3%（63眼）、90.6%（52眼）、85.9%（40眼）、85.9%（23眼）、85.9%（15眼）。出生后1月内手术者手术成功率、术后视力高于出生1月后手术者（P=0.033；P=0.01）。未出现滤过泡渗漏、滤过泡炎、眼球萎缩等严重并发症。【结论】NPCG患儿早期行小梁切开或小梁切开联合小梁切除术手术安全、疗效确切

青少年型开角型青光眼滤过手术的长期临床疗效

目的探讨青少年型开角型青光眼小梁切除术长期疗效。方法回顾性研究收集18岁以内接受小梁切除术联合丝裂霉素C（MMC）的青少年型开角型青光眼共31例55眼，比较手术前后眼压及其他眼部参数。结果所有患者术后眼压比术前明显降低（t=12.254，P＜0.001），手术前后C/D值、眼轴长度、视野MD值、视力及屈光度无明显变化，术后第1、3、6年手术完全成功率分别为92.7%、70.4%、42.6%。手术条件成功率分别为92.7%、81.0%、81.0%。术中及术后未发生严重并发症。结论小梁切除术联合MMC是治疗青少年型开角型青光眼一种较为安全、成功率较高的手术方式

多元文化经历促进创造力

大量实证研究支持了多元文化经历促进创造力这一假设。深度参与两种不一致的文化活动是多元文化经历提升创造力的前提条件; 在融合过程中, 双重认同者获得的交替、整合和包含等认知技能, 促进了个体的创造性表现。未来可以采用更具多样性的样本以扩展研究的外部效度, 考察认知评价、趋近动机、积极情绪的中介作用, 并加强国内对此领域的研究

Multicultural experience fosters creativity

大量实证研究支持了多元文化经历促进创造力这一假设。深度参与两种不一致的文化活动是多元文化经历提升创造力的前提条件; 在融合过程中, 双重认同者获得的交替、整合和包含等认知技能, 促进了个体的创造性表现。未来可以采用更具多样性的样本以扩展研究的外部效度, 考察认知评价、趋近动机、积极情绪的中介作用, 并加强国内对此领域的研究。</p

Report on China advertising education current situation

本研究采用各种信息搜集手段对我国高校广告专业的创办情况、生源、师资队伍和课程设置进行调查,结果显示:经过1992和1999年是两次飞跃性的发展,设有广告专业的高等院校已经由1983年的1所发展到2007年的322所;57.89%的985院校,46.73%的211学校都设有广告专业;广告专业学生以文科为主,兼顾理科和艺术类;广告师资队伍年轻、学历和职称偏低、经验和专业性不足;课程设置基本合理,但仍有随意性。【英文摘要】All kinds of means were applied to collect the data of the advertising major of all the universities ,in terms of the circumstances of the ma-jor,the students’ source,the quality of teachers and the curriculum settings. The results showed that after the twice leap-forward development occurred respectively in 1992 and 1999,the universities with advertising majors have increased in number from 1 in 1983 to 322 in 2007. Among all the universities of 985 Project,57.89% with the advertising major,while among all the universitiesof 985 Project, 57.89% with the advertising major, while among all the universities of 211 Project, 46.73% has the advertising major. The advertising majorsare composed of the arts, the sciences and the fine arts, while the advertising teachers are young, with relatively low academic titles and lack of experienceand professionalism. The curriculum settings are reasonable on the whole, but randomness still can be found in some curriculum settings.steps ofintemational advertising , it will give new impetus to the growth of China’s market economy.中国教育部与电通公司“电通·中国广告人才培养基金项目”中国广告教育委托研究项目资

水酶法提取栀子油工艺优化及其脂肪酸组成Optimization of extraction of Gardenia jasminoides oil by aqueous enzymatic method and its fatty acid composition

为促进栀子的开发利用，以栀子成熟果实为原料，采用水酶法提取栀子油。采用单因素实验研究酶种类、加酶量、酶解pH、酶解温度、液料比、酶解时间对栀子油得率的影响，在此基础上采用均匀设计实验进行工艺条件优化，并对各种酶提取的栀子油进行脂肪酸组成分析。结果表明：水酶法提取栀子油的最佳工艺条件为采用中性蛋白酶、加酶量0.7%、液料比3∶ 1、酶解pH 7、酶解温度60 ℃、酶解时间7 h，在此条件下栀子油得率为7.27%，与空白组（3.34%）相比提高了117.66%；栀子油中亚油酸含量最高，超过56%，不饱和脂肪酸含量为80%左右。不同酶提取栀子油的脂肪酸组成及含量没有显著差异。 In order to promote the development and utilization of Gardenia jasminoides, With the mature fruit of Gardenia jasminoides as raw material, the oil was extracted by aqueous enzymatic method. The effects of enzyme species, enzyme dosage, enzymatic hydrolysis pH, enzymatic hydrolysis temperature, liquid-material ratio and enzymatic hydrolysis time on the yield of Gardenia jasminoides oil were studied by single factor experiment. The process parameters were optimized by uniform design on the basis of single factor experiment. The fatty acid composition of Gardenia jasminoides oil extracted by different enzymes was analyzed. The results showed that the optimal process conditions were obtained as follows: with neutral protease for enzymolysis, enzyme dosage 0.7%, liquid-material ratio 3∶ 1, enzymatic hydrolysis pH 7, enzymatic hydrolysis temperature 60 ℃ and enzymatic hydrolysis time 7 h. Under these conditions, the yield of Gardenia jasminoides oil was 7.27%, which was 117.66% higher than that of control(3.34%). The linoleic acid content in Gardenia jasminoides oil was the highest, exceeding 56%, and unsaturated fatty acids content was about 80%. There was no significant difference in the fatty acid composition and content of Gardenia jasminoides oils extracted by different enzymes

Measurement of integrated luminosity of data collected at 3.773 GeV by BESIII from 2021 to 2024

We present a measurement of the integrated luminosity e+e- of collision data collected by the BESIII detector at the BEPCII collider at a center-of-mass energy of Ecm = 3.773 GeV. The integrated luminosities of the datasets taken from December 2021 to June 2022, from November 2022 to June 2023, and from October 2023 to February 2024 were determined to be 4.995±0.019 fb-1, 8.157±0.031 fb-1, and 4.191±0.016 fb-1, respectively, by analyzing large angle Bhabha scattering events. The uncertainties are dominated by systematic effects, and the statistical uncertainties are negligible. Our results provide essential input for future analyses and precision measurements