Development of quantitative microparticle immunoassay for human serum ferritin based on full-automatic chemiluminescence immune analyzer

缺铁性贫血（irondeficiencyanemia，IDA）是由于各种不同的原因导致体内储存铁缺乏，影响细胞血红素的生成而导致的贫血。缺铁性贫血是世界范围内最常见的营养缺乏病之一，影响着约10亿人口。 从缺铁发展到缺铁性贫血是一个缓慢的过程，一般经历铁缺乏期（irondeplete,ID）、缺铁性红细胞生成期（irondeficiencyerythropoiesis，IDE）和缺铁性贫血期。发生缺铁和缺铁性贫血不仅会影响身体正常的机能，还可能导致胎儿和儿童的智力发育受到不可逆转的影响。通常机体内储存铁的量与血清铁蛋白的水平成正相关，机体内血清铁蛋白的水平是评价机体储存铁缺乏和诊断缺铁性贫血...Iron deficiency anemia (IDA) is a type of anemia caused by not having enough storage iron in body because of various reasons. Iron deficiency anemia is one of the most common nutrition deficiency diseases, affecting about 1 billion people in the world. The progress from iron deficiency to iron deficiency anemia is a slow process, which can divided into three stages: iron deplete(ID), iron deficie...学位：理学硕士院系专业：生命科学学院_细胞生物学学号：2162011115234

双能谷效应对N型掺杂Si基Ge材料载流子晶格散射的影响

性能优越的Si基高效发光材料与器件的制备一直是Si基光电集成电路中最具挑战性的课题之一.Si基Ge材料不仅与成熟的硅工艺相兼容,而且具有准直接带特性,被认为是实现Si基激光器最有希望的材料.对Si基Ge材料N型掺杂的研究有利于提示出其直接带发光增强机理.本文研究了N型掺杂Si基Ge材料导带电子的晶格散射过程.N型掺杂Si基Ge材料具有独特的双能谷（Γ能谷与L能谷）结构,它将通过以下两方面的竞争关系提高直接带导带底电子的占有率:一方面,处于Γ能谷的导带电子通过谷间光学声子的散射方式散射到L能谷;另一方面,处于L能谷的导带电子通过谷内光学声子散射以及二次谷间光学声子散射或者直接通过谷间光学声子散射的方式跃迁到Γ能谷.当掺杂浓度界于1017cm-3到1019cm-3时,适当提高N型掺杂浓度有利于提高直接带Γ能谷导带底电子占有率,进而提高Si基Ge材料直接带发光效率.国家自然科学基金青年基金(批准号:61604041);;福建省自然科学基金青年基金(基金号:2016J05147);;福建省教育厅2017年高校杰出青年科研人才培育计划项目;;福建工程学院校科研启动基金(批准号:GY-Z14073)资助的课题~

间歇式轴向压应力对组织工程骨种子细胞的黏附增殖与成骨分化促进作用的研究

目的探究间歇式轴向压应力对组织工程骨种子细胞黏附、增殖与成骨分化能力的影响。方法构建表达绿色荧光蛋白的兔骨髓间充质干细胞(rBMSCs)作为示踪种子细胞,运用旋转细胞培养仪将松质骨支架和种子细胞共培养7 d获得组织工程骨(TEB),实验组在第7～14天施加大小10 N、频率1 Hz、4 h/d的间歇式轴向压应力刺激,对照组常规培养,14 d后胰酶消化法获取两组种子细胞并比较其黏附、增殖和成骨分化能力。采用两组独立样本t检验进行统计学分析。结果 (1)流式细胞术显示rBMSCs被成功提取分离。(2)倒置荧光显微镜及扫描电镜显示TEB中种子细胞与支架相容性良好。(3)活体荧光成像系统及扫描电镜显示应力刺激组种子细胞的生长状况要优于非应力刺激组,前者平均荧光密度及细胞数/500倍视野均大于后者,差异均具有统计学意义(平均荧光密度:(3.75±0.34)×108 vs(2.91±0.22)×108,t=2.90,P=0.04;细胞数/500倍视野:30.50±4.43 vs 21.00±5.13,t=3.14,P=0.01)。(4)细胞黏附实验显示,应力刺激组种子细胞的75﹪细胞贴壁时间短于非应力刺激组,两组时间分别为(3.00±0.41) h、(13.33±1.70)h,差异具有统计学意义(t=8.20,P <0.01),前者的最终细胞贴壁率高于后者(99.97﹪±0.34﹪vs 85.83﹪±1.18﹪),差异具有统计学意义(t=11.31,P <0.01)。(5) CCK-8检测显示,在培养第48～96 h,应力刺激组种子细胞的增殖能力优于非应力刺激组,将两者的450 nm吸光度值在第48小时(0.49±0.02、0.40±0.02)、72 h(0.76±0.07、0.64±0.04)和96 h(1.58±0.07、1.34±0.13)分别进行比较,差异均具有统计学意义(t=5.15、2.57、2.86,P均<0.01)。(6)在成骨诱导14 d后,应力刺激组种子细胞的ALP和Ca结节染色阳性率要强于非应力刺激组:两组ALP染色阳性率分别为26.73﹪±4.56﹪、16.68﹪±3.89﹪,差异具有统计学意义(t=3.33,P=0.03);两组Ca结节染色阳性率分别为41.81﹪±3.56﹪、27.40﹪±2.35﹪,差异具有统计学意义(t=3.68,P=0.02)。结论间歇性轴向压应力可促进组织工程骨种子细胞的黏附、增殖与成骨分化

Studied the difference of genetic feature between tissue cultured and cultivated paddy rice of Jia He Zao Zan via microsatellite(SSR) markers

株高是水稻重要的农艺形状之一,植株过高将导致倒伏和减产,目前,很多新的技术究被用来鉴定,图位克隆与水稻株高相关的基因及机理的研究,本实验选择优质早籼稻佳禾早占种植苗和经过组培获得的矮化突变水稻为材料,为研究比较它们间的遗传物质上差异,根据康耐尔大学的资料设计了311对SSR引物对佳禾早占种植材料和组培材料进行分析,对两种材料进行PCR多态性扩增,结果发现两者间存在多态性的引物有88对,多态性比例达到30.3%。在矮杆材料中不但验证了已报道的11个与调控株高性状基因相连锁的标记连锁群,同时在第3号染色体和第9号染色体上还获得了两个以前基本未有报道的标记集中分布区域。结果说明,该培养基培育出的佳禾早占水稻后代所表现出的矮杆性状与亲本在遗传物质上确有明显差别。该结果有助于挖掘和定位新的矮杆基因,并有利于今后在水稻育种中进行水稻株高性状的控制,同时也为开展矮化性状机理的研究提供有利的实验材料。Plant height is one of the important agronomic traits of rice.Overhigh plant easily led to lodge and reduce output.Recently,great advances have been made in the identification,mapping,cloning and action mechanism of plant height genes in rice.This present paper selected the tissue cultured and cultivated paddy rice of Jia He Zao Zan(J.H.Z.Z) as experimental object,which were significant different on the height,the tissue cultured plant was only less than half height of the cultivated plant.In order to compare their differences of genetic and determine whether there are relationship between the dwarf gene and the dwarf character of tissue cultured plant,Polymorphism analysis was conducted between them by 311 pairs of rice SSR primers,designed by Cornell University,the result indicated that 88 pairs of primers had polymorphism between two kinds of materials,the ration in total primers was about 30.3%,the experimental result not only verified 11 multiple groups which regulate the height gene,but also obtained two group regions which control the dwarf character on chromosome 3 and chromosome 9,which have not been reported previously,these results will help to explore and determine the situation of new dwarf gene,and will be of benefit to act genetic manipulation of plant height of in rice breeding.These results also provided the evidence for studying the mechanism of dwarf.国家863项目(2002AA211091),(20001AA11091);; 厦门大学细胞生物学和肿瘤细胞工程教育部重点实验室开放基金资助(NO:2005108)资

Development of chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate

目的研制硫酸脱氢表雄酮化学发光免疫定量检测试剂。方法利用硫酸脱氢表雄酮人工完全抗原免疫小鼠,通过杂交瘤技术制备特异性抗硫酸脱氢表雄酮单克隆抗体,采用竞争抑制法建立硫酸脱氢表雄酮化学发光免疫定量检测试剂。结果筛选获得了27株稳定分泌抗硫酸脱氢表雄酮的单克隆抗体细胞株,建立了化学发光微粒子免疫法定量测定硫酸脱氢表雄酮的试剂盒雏形,与雅培公司的硫酸脱氢表雄酮定量检测试剂在检测临床标本上的相关系数r达0.99以上。结论本研究为国产化硫酸脱氢表雄酮化学发光微粒子免疫法定量测定试剂盒的研发奠定了基础。Objective To develop chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate.Methods Mice were immunized by dehydroepiandrosterone sulfate artificial complete antigen.Monoclonal antibodies against dehydroepiandrosterone sulfate were prepared by hybridoma technique.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate was developed based on competitive binding method.Results 27 monoclonal antibodies were obtained.Chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate prototype kit was established.The coefficient r of testing specimens with reagent from Abbott company was over 0.99.Conclusion Development of domestic chemiluminescent microparticle immunoassay for quantitative determination of dehydroepiandrosterone sulfate reagent was laid foundation by this study.国家高技术研究发展计划(863计划)(2011AA02A101

(001)面双轴应变锗材料的能带调控

本文基于形变势理论构建(001)面双轴应变Ge材料的能带结构模型。计算结果表明(001)面双轴应变可以将Ge的能带从以L能谷为导带底的间接带半导体调控到以Δ4能谷为导带底的间接带半导体或者以Г能谷为导带底的直接带半导体。同时室温下Ge的带隙与应变的关系可用四段函数来表示:当压应变将Ge材料调控为以Г能谷为导带底的间接带半导体后,每增加1%的压应变,禁带宽度将线性减小约78.63meV;当张应变将Ge材料调控为直接带半导体后,张应变每增加1%,禁带宽度将线性减小约177.98meV;应变介于-2.06%和1.77%时,Ge将被调控为以L能谷为导带底的间接带半导体,禁带宽度随着压应变每增加1%而增加11.66meV,随着张应变每增加1%而线性减小约88.29meV。该量化结果可为研究和设计双轴应变Ge材料及其器件提供理论指导和实验依据。国家自然科学基金资助项目(61604041);;福建省自然科学基金资助项目(2016J05147);;福建省教育厅2017年高校杰出青年科研人才培育计划项目;;福建工程学院科研基金资助项目(GY-Z14073

基于Pro/E和ANSYS的摆线齿轮泵模态分析

运用三维CAD软件Pro/E建立了某型摆线齿轮泵三维几何模型,然后用Hypermesh对其进行网格划分,简化并导入ANSYS软件中,建立了有限元模型。利用ANSYS软件对摆线齿轮泵进行了模态分析,获得摆线齿轮泵的振型、固有频率和振型参与系数,确保在设计摆线齿轮泵时能够避免模型发生共振。分析结果表明摆线齿轮泵的结构设计合理,而且模态分析的结果可以为新产品的开发和结构优化设计提供重要的参考依据

Electromagnetic Design and Optimization of Superconducting Spoke021 Cavity for C-ADS

为满足C-ADS 项目建设对超导轮辐腔Spoke021的需求，对Spoke021进行了详细的电磁参数优化。对Spoke021的参数化模型进行参数扫描，针对腔体的关键电磁特征量寻找可能存在的极值，详细分析、解释了优化过程中各个参数发生变化的物理意义。在Spoke021各参数达到最终优化值时，表征腔体性能的两个关键比值分别为：E_p/E_(acc) = 3:14，B_p/E_(acc) = 4:77 mT/(MV/m)。考虑到次级电子倍增(Multipacting，MP)对Spoke021运行中所能达到的性能指标有很重要影响，对腔体的MP进行了建模分析。结果表明，当Spoke021工作在Eacc =10 MV/m情况下，没有发生MP，优化得到的参数可以满足Spoke021工程设计的需要；最后计算了腔体的TTF曲线，表明该腔体具有较宽的速度接受度。<div> The electromagnetic optimizations of a superconducting Spoke021 cavity is studied in detail for C-ADS project, in this paper. The cavity model has been parameterized, in order to find the possible maxima or minima of the key electro-magnetic parameters, the parameter sweep is performed. The cavity model has been parameterized. For finding the key electromagnetic feature, the geometric parameter sweep is performed. The physical interpretation for the variation of these electromagnetic parameters has been elaborated. The electromagnetic simulation gives the optimum parameters E_p/E_(acc) of 3.14 and B_p/E_(acc) of 4.77 mT/(MV/m). In addition, the Multipacting(MP) has a great effect on the electromagnetic properties of superconducting Spoke021 cavity. So the MP simulations have been done for this cavity, which shows that there is no MP phenomenon at 10 MV/m accelerator gradient. Therefore, the optimized cavity parameters can satisfy the requirements of the C-ADS project. Finally, the TTF(Transit Time Factor) curve of this cavity is calculated and it shows that this cavity has wide velocity acceptance.</div> <div> &nbsp;</div>中国科学院战略性先导科技专

Study of nano-intercalation amino resin superficial modified tunnel fire proof coating

作者简介: 张小玲(1959-),女,厦门市消防支队 防火处高级工程师,主要从事消防监督管理工作, 福建 省厦门市湖滨北路119 号, 361012。[中文文摘]通过对主粘结剂、聚合物改性剂、骨料和无机阻燃剂等因素的研究,得到改进的常规厚涂型防火涂料,在其表面涂覆纳米插层氨基树脂以改善其耐腐蚀性。以原位插层法制备了蒙脱土/氨基树脂纳米复合材料,采用X射线衍射(XRD)、断面扫描电镜(SEM)及透射电镜(TEM)研究了其分散形态。试验表明通过表面涂覆改性得到的隧道防火涂料组合物具有良好的耐腐蚀性和耐火性能。[英文文摘]Via the investigation on factors such as main adhesive, polymeric modifier, bond materials and inorganic fire retardant, the performance of thick coated fire proof coating is improved. Surface modification by nano-intercalation amino resin improves the anti-corrosion property of the fire proof coating. The montmorillonite/amino resin nano-meter composite is made by the method of in-situ intercalation, and its dispersion morphology is analyzed by XRD, SEM and TEM. The experiments prove that the nano-intercalation amino resin modified fire proof coating performes well in fire proof and anti-corrosion