欧鳗“狂游症”病调查报告

欧鳗“狂游症”病调查报告黄印尧陈信忠颜江华（厦门动植物检疫局361012）（厦门大学抗癌研究中心361021）近年来，我国从欧美引进了大量的鳗苗，但欧鳗的病害，尤其是以狂游衰竭而死亡为主要特征的“狂游症“病常常造成全场鳗鱼发病死亡，死亡率达90%以上..

狂犬病抗体免疫金标检测试纸的研制

目的应用酶联免疫原理和胶体金层析技术,研制狂犬病抗体免疫金标检测试纸。方法采用特殊的生产工艺,在玻璃纤维膜包被胶体金标记狂犬病抗原,在硝酸纤维素膜上检测线和对照线处分别包被狂犬病抗原和兔抗狂犬病抗体,制成狂犬病抗体免疫金标检测试纸(人用)。结果当待检样品阳性时,在检测线处形成抗原抗体的免疫复合物而凝聚显色;当待检样品阴性时,检测线处不形成抗原抗体免疫复合物不显色。整个试验过程只需15 m in。试纸与ELISA试剂比较,两者都具有微量、特异、准确的优点,且金标试纸独具操作方便、快速和结果直观、容易判定的优点。结论应用胶体金免疫层析技术建立的"狂犬病抗体免疫金标检测试纸",可以准确地检测出被检样品是否带有狂犬病抗体,同时还可以通过检测线颜色的深浅判定抗体的含量高低

猪瘟病毒EO蛋白的原核表达及其重组蛋白活性测定

通过重叠PCR合成猪瘟病毒EO基因,将该片段定向插入到pET-22b载体中,构建原核表达载体pET-22b／EO,转化大肠杆菌BL21 (DE3),IPTG诱导表达,比较不同诱导条件下的蛋白表达,确定其最佳表达条件。重组蛋白主要以包涵体的形式表达,Ni2+亲和层析柱纯化蛋白,逐步透析法复性。通过方阵试验确定包被抗原的最适工作浓度,为了测定EO蛋白的活性,本文初步建立了检测猪瘟血清抗体水平的间接 ELISA方法,为开发检测猪瘟抗体诊断试荆奠定基础

口蹄疫病毒3AB基因的表达及活性分析

通过重叠PCR合成口蹄疫病毒3AB基因,构建原核表达载体pGEX-5X-1/3AB,转化大肠杆菌BL21(DE3),IPTG诱导表达。重组蛋白主要以包涵体的形式表达,将包涵体洗涤溶解后,采用Na2+离子金属螯合亲和层析柱纯化,逐步透析法复性。ELISA实验表明,目的蛋白能与猪口蹄疫阳性血清发生特异性反应。本研究为建立以基因工程产品为抗原、鉴别诊断自然感染和免疫动物的方法提供了技术条件

猪圆环病毒2型抗体免疫金标检测试纸的研制

应用酶联免疫原理和胶体金层析技术,采用特殊的生产工艺,在玻璃纤维膜包被胶体金标记PCV-2抗原,在硝酸纤维素膜上检测线和对照线处分别包被PCV-2抗原和兔抗PCV-2抗体,制成猪圆环病毒2型抗体免疫金标检测试纸。当待检样品阳性时,在检测线处形成抗原抗体的免疫复合物而凝聚显色;当待检样品阴性时,检测线处不形成抗原抗体免疫复合物不显色。整个试验过程只需15min。试纸与ELISA试剂比较,两者都具有微量、特异、准确的优点,且金标试纸独具操作方便、快速和结果直观、容易判定的优点

Study and Apply for Detection of PRRS Anti-body by ColloidalGgold Stripe

应用酶联免疫吸附原理和胶体金层析技术,在玻璃纤维和硝酸纤维素膜的检测线和对照线上分别喷上胶体金PRRSV,PRRSV和兔抗PRRS抗体,从而制作成猪PRRS抗体免疫金标试纸,用该试纸与PRRS-ELISA试剂盒分别对30份猪血清及血液样品进行抗体水平检测,结果完全一致。说明金标试纸法是一种微量、特异、简便和结果容易判定的新的检测方法,非常适用于基层兽医站和猪场,特别是进行现场检测和开展大规模疫情普查时使用。Based on the principle of immune - ELISA and colloidal gold immunochromatography, colloidal gold stripe was prepared and applied toto the detection of anti - PRRS antibody. Gold PRRSV, PRRSV and anti - PRRS antibody was coated on the test region and control zone respectively.Anti - PRRS antibody in 34 whole blood and sera sample was detected by the strip and PRRS - ELISA method. Result achieved from the methods mentioned above are in complete agreement. It shows that the method established in this paper bears the merit of micro analysis, specificity, conveniece and the result is easy to be tested. This method is applicable for the detection of anti - PRRS antibody in a wide area and can be applied to general survey of PRRS disease

The Pathogenicity and its Ultrastructural Evidence of Trionyx Sinensis Virus

本文应用流行病学调查方法和电镜超薄切片技术.对福建省4个中华鳖养殖场的病毒件传染病进行观察，发现在病鳖肝细胞中的中华鳖病毒（TSV）是导致幼鳖大批死亡的主要致病原.还讨论了该病毒的超微结构以及对宿主细胞的损害情况。One viral disease of Trionyx sinensis had been investigated in 4 cultured regions in Fujian prevince by using epidemiological investigation and ultrathin section of the transmission electron microscope.The spherovirus infecting hepatocyte was the major Pathogen which make a lot of young turtles to death.The virus's ultrastructure and damaging effect to host cells had also been discussed

Study and apply for detection of pestissuum antibody by colloidal gold strip

以猪瘟抗原包被硝酸纤维素膜的检测区,在其下端附着胶体金标记的猪瘟抗原,组成免疫金标试纸条,根据胶体金免疫层析原理,用该试纸条建立检测猪瘟抗体水平的免疫金标检测法。再用本试纸条检测法与Dot-ELISA及间接血凝法对298份猪、鸡、鸭、鹌鹑、兔、鼠、羊血清进行猪瘟抗体检测,结果符合率达100%。试验结果表明,本法与Dot-ELISA及间接血凝法一样特异、敏感和微量,而且检测时间短、直观,结果容易判定,适用于大面积猪瘟抗体监测普查。Based on the principle of colloidal g old im-munochromatography,a strip employ ing colloidal gold technique was prepared and applied t o the detection of an-ti -pestissuum antibody.Detection region of the cellulose membrane was coated by pestissuum an tigen.Which is an bad with colloidal gold wsa attached in the area under the detection region.Anti -pestissuum antibody in 298sera sample obtained from pig,chicken,d uck,quail,rabbitand goat was detected by the strip,Dot -ELISA and indirect agglunation method.Result achieved from the methods mentioned above are in complete agre ement.It shows that the method established in this paper bears the same merits as that of the Dot -ELISA and indirect agglunation meth-ods,which is as follows:It can be emp loyed in micro -analysis and observed directly,the result is easy to be jus-tified;it is time saving,and with hi gh speccifity and sensi-tivety.This method is applicable fo r the detection of pestissuum antibody in a wide areas a nd can be applied to the general survey of pestissuum dis ease

Study and Apply of Colloidal Gold Strip in Detecting the Antibody of pseudorabies

应用免疫学原理与胶体金层析技术相结合 ,采用双抗原夹心法建立了检测猪伪狂犬病抗体水平的“猪伪狂犬病抗体免疫胶体金快速检测试纸法” ,用该法与美国Idexx公司的猪伪狂犬病ELISA试剂盒 ,同时对 1 6份猪血液或血清进行抗体水平检测 ,符合率达 1 0 0 %。同时使用金标法对 1 0 0份猪血液和对应血清进行检测 ,结果也相同。试验结果显示 ,金标法与ELISA一样 ,具有微量、特异、准确等优点 ,且操作简易 ,而金标法不需要任何仪器 ,检测时间短 ,结果直观 ,容易判定 ,适用于基层兽医站、养猪场使用和大面积猪伪狂犬病抗体普查。Based on the principle of colloidal gold immunochromatography, a test strip was prepared and applied in detecting the antibody of pseudorabies. The test result was coincidence with ELISA made in USA Indexx cor. in examination of 80 blood or sera samples from pig、chicken、 duck、 cattle and goat. It shows that this strip has the same merits as that of ELISA test in microanalysis, high specialty and accurate. No special instrument is needed and the test can be completed easily in a short time. So it can be used widely in common veterinary services, hoggery and the general survey of pseudorabies disease

Adiagnostic and Cure Report on the Viral Disease of Haliotis divericolor aquatilis

1999～ 2 0 0 0年冬春季节。福建省东山、漳浦养殖九孔鲍鱼暴发急性、毁灭性传染病流行。经过一系列实验室检验和病例复制试验 ,证实它是由一种大小 5 0～ 80 x12 0～ 15 0 nm球状病毒侵袭鲍体所引起的。该病采取隔离消毒、净水养殖、药浴和饲喂药物饲料 ,可以起到很好的防制作用A spherovirus around 50 80 x 120 150nm in fecting Haliotis divericolor aquatilis is proved as a pathogen of a fatal infectious diease which had occurred on many abalone feedlots in Dongshan and Zhanpu of Fujian province in recent years by using laboratory examination and case replication.This disease could be cured and control its pervasion by using isolation method,breeding in health water,bathing and feeding with some drugs