Effect of edaravone on cerebral edema and lipid peroxidation in rats of cerebral hemorrhage

目的探讨依达拉奉对大鼠脑出血后脑水肿及脑组织氧化损害的影响。方法选取健康雄性清洁的SD大鼠150只,随机分为假手术组、对照组、治疗组,每组50只,分别于术后0.5、1、2、3、4 d 5个时间点对每组中不同10只进行观察。采用立体定向自体血注入法制作大鼠脑出血模型,检测3组并比较各时间点血肿周围脑组织含水量变化、丙二醛(MDA)含量变化及超氧化物歧化酶活力情况。结果假手术组和治疗组术后1~4 d各时间点血肿周围脑组织含水量均显著低于对照组(P<0.05);对照组在术后2d脑水肿达到最高值;假手术组、治疗组术后0.5~4 d各时间点血肿周围脑组织MDA含量均显著低于对照组(P<0.05);假手术组、治疗组术后0.5~4 d各时间点血肿周围脑组织SOD活力均显著高于对照组(P<0.05)。结论依达拉奉能明显改善脑出血大鼠血肿周围脑组织水肿,减轻脑出血大鼠脑组织氧化损害。Objective To discusse the effect of edaravone for brain tissue oxidative damage after cerebral hemorrhage through observing the hemorrhage rats back edema and the change of lipid peroxidation. Methods 150 healthy and clean male SD rats,were randomly divided to sham operation group,control group,treatment group,50 cases in each group,each group choose five time points,post operative 0. 5 day,1 day,2 days,3 days,4 days,observed different 10 rats at each time point. The intracerebral hemorrhage model was made by using the method of stereotactic autologous blood injection. The changes of water content,the content of MDA and the activity of superoxide dismutase were monitored in three groups at different time points. Results Surrounding the hematoma in the brain tissue around the hematoma after intracerebral hemorrhage in sham operation group,treatment group of postoperative day 1-4 days each time hematoma surrounding brain tissue water content was significantly lower than that in control group( P <0. 05); Control group after 2 days of cerebral edema and reached the highest value; Sham operation group and treatment group of postoperative half of the day to 4 days each time the content of MDA were significantly lower than in control group( P < 0. 05); Sham operation group and treatment group postoperative half of the day to 4 days each time the brain tissue SOD activity was significantly higher than control group( P < 0. 05). Conclusion The effect is obvious that edaravone for cerebral hemorrhage rats brain tissue around hematoma edema,reduce cerebral hemorrhage rat tissue oxidative damage.福建省医学创新课题项目(2015-1-85

两极化趋势下人民币汇率制度的选择

自20世纪90年代以来,汇率制度的变革出现两极化趋势,越来越多的国家选择自由浮动或完全固定的汇率制度,而“软”的钉住汇率制度正逐步被抛弃。本文在“两极论”的基础上对固定汇率制度与浮动汇率制度的取舍进行分析,并结合我国国情探讨两极化趋势下人民币汇率制度的选择

昆虫抗菌肽研究和应用现状

昆虫在受到刺激或感染之后，在其血淋巴中会产生一种抗菌类物质，称抗菌肽。抗菌肽具有分子小、稳定性好、广谱抗菌、无毒副作用等特点，在农业、医药、食品等领域有广泛的应用前景。简要综述了昆虫抗菌肽的基础研究和应用现状

昆虫抗菌肽研究和应用现状

昆虫在受到刺激或感染之后,在其血淋巴中会产生一种抗菌类物质,称抗菌肽。抗菌肽具有分子小、稳定性好、广谱抗菌、无毒副作用等特点,在农业、医药、食品等领域有广泛的应用前景。简要综述了昆虫抗菌肽的基础研究和应用现状

抗菌肽数据库简介

随着抗菌肽研究的不断发展，科研工作者建立了抗菌肽数据库。这些数据库使得抗菌肽的研究更加方便。就几个重要抗菌肽数据库的内容和使用方法作一简介

抗菌肽数据库简介

随着抗菌肽研究的不断发展,科研工作者建立了抗菌肽数据库。这些数据库使得抗菌肽的研究更加方便。就几个重要抗菌肽数据库的内容和使用方法作一简

Regulation of glucocorticoids on plasma tumor necrosis factor-α and interleukin-1β in brain trauma

目的研究糖皮质激素(glucocorticoid,GC)对脑外伤患者血浆肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)及白细胞介素1β(interleukin-1β,IL-1β)的调控作用,指导临床合理用药。方法随机选取2013年4月~2016年8月入住厦门大学附属第一医院神经外科的脑外伤患者120例,依随机表对照法随机分为激素组60例和非激素组60例,另外选择60例健康的体检者作为对照组。激素组给予常规剂量的地塞米松治疗1周。对照组不做任何处理。采用酶联免疫吸附法(ELISA)测定2组患者在治疗后第1、2、7、14天时血浆中TNF-α和IL-1β的含量。结果在第1、2天激素组的血浆中TNF-α和IL-1β的含量明显高于对照组(P<0.05),与非激素组差异无统计学意义。而在第7、14天明显低于非激素组(P<0.05),与对照组无明显差异。结论糖皮质激素对脑外伤患者TNF-α和IL-1β的调控具有明显的滞后性,损伤早期不能及时降低TNF-α和IL-1β的含量,在恢复期又使其降低,削弱了其对神经的保护作用,这可能会加剧患者的病情。Objective To study the regulation of glucocorticoid( GC) on plasma tumor necrosis factor-α( TNF-α) and interleukin-1β( IL-1β) in patients with brain trauma and guid rational drug use. Methods 120 patients with brain trauma were randomly selected from April 2013 to August 2016 in our hospital,according to a random table were divided into hormone group and non-hormonal group,60 cases of each group,another 60 healthy volunteers as control group. The hormone group received dexamethasone for one week,the control group with no treatment. The plasma TNF-α and IL-1βlevels on 1st,2nd,7thand 14 thdays were compared between two groups. Results On the 1stand 2ndday,plasma TNF-α and IL-1β levels in hormone group were significantly higher than control group( P < 0. 05),while there was no significant difference compared with non-hormone group. On 7thand14 thdays,the TNF-α and IL-1β levels in hormone group were significantly lower than non-hormone group( P < 0. 05),while there was no significant difference compared with control group. Conclusion The regulation of GC on TNF-α and IL-1β was hysteretic in traumatic brain injury patients,and can not reduce TNF-α and IL-1β content timely,but reduce their levels during the recovery period instead,reduce their protective effects of nerve,which could exacerbate the patient's condition.福建省医学创新课题(2015-1-85

准噶尔无叶豆地理种群种子萌发特性

准噶尔无叶豆种子具有明显的地理种群大小变异的现象。对大、小种子在3种光照(持续光照,12/12小时光照/黑暗,全黑暗)、5个温度梯度(10℃,15℃,20℃,25℃,30℃)条件下的最适萌发条件进行研究,结果表明:大、小种子萌发的适宜光照条件均为12/12小时光照/黑暗,在持续黑暗与持续光照条件下的萌发率无显著差异;最适温度范围是20~25℃,大种子在20℃、25℃下的萌发率(>85%),萌发指数均显著高于相同温度条件下小种子的萌发率(<35%)和萌发指数。大种子存在种皮硬实所导致的物理性被迫休眠,而小种子除物理性休眠外,可能还存在其他形式的深度生理休眠。准噶尔无叶豆自然状态下萌发率极低(≤3%),经综合分析,认为种子硬实和深度生理休眠可能是自然状态下种子萌发的困难主要因素,而土壤的光照、温度及湿度不存在明显的对种子萌发的限制

新疆三工河流域冲洪积平原区潜水蒸发特征与潜水调控

利用三工河阜北地下水潜水均衡试验场资料,对该区潜水有效蒸发期4~10月的潜水蒸发量特征进行了分析,提出了该区潜水埋深调控范围

Chirality Transfer in Alanine Based N-amidothioureas and Their Anion Complexes

通过改变丙氨酸n端的取代基,设计合成了2种基于丙氨酸残基的酰胺基硫脲衍生物.通过X-射线单晶衍射、核磁共振波谱、吸收光谱和圆二色(Cd)光谱等方法研究了2种不同取代基对硫脲分子自身及其阴离子络合物的氢键网络的影响以及由此引起的手性传递能力的不同.实验表明,当丙氨酸n端取代基为n,n-二甲基甘氨酸残基时,其羰基氧能与硫脲基团中的nH以分子内十元环氢键结合,分子呈折叠构象,实现了手性从丙氨酸残基到苯基硫脲基团的传递.阴离子加入后,该分子的硫脲基团与阴离子以双重氢键结合,分子原有的氢键网络发生改变,羰基氧与酰胺氮氢(nH)之间七元环氢键的存在将手性更多的限制于丙氨酸残基部分,手性传递至阴离子结合部位的效率极低.而n端被二甲基取代的硫脲衍生物由于分子内不同的氢键网络而表现出不同的手性传递能力:结合阴离子前,分子本身不具有折叠构象而无手性传递发生,结合阴离子后,新的氢键网络诱导了手性信号的长程传递.Two different N-amidothioureas bearing substituents at the N terminus of alanine residue were synthesized.The influence of the substituents on the hydrogen bonding networks and chirality transfer in both the N-amidothioureas and their anion binding complexes were also investigated by single-crystal X-ray diffraction,NMR,UV-Vis,and circular dichroism(CD).Our study reveals that the hydrogen bonding between the oxygen atom of the substituent at the N terminus and the thiourea NH makes the molecule fold and promotes chirality transfer from the alanine residue to the thiourea moiety.Similar influence of the substituents is also observed in the thioureas anion binding complexes.The hydrogen bonding formed between the oxygen atom of the substituent and the amido NH limits the chirality transfer to the anion binding moiety,while the molecule with the dimethyl substituent at the N terminus has different chirality transfer ability due to different hydrogen bonding network.国家自然科学基金(91127019;21275121;J1030415); 国家重点基础研究发展计划(973)项目(2011CB910403