单个纳米颗粒的光散射检测技术进展

纳米颗粒因其在生物医学和生物分析领域具有重要的应用前景而备受关注.单个纳米颗粒的光散射检测技术是一种简单、有效地对纳米颗粒的尺寸、尺寸分布及浓度等进行表征的分析方法,尤其在揭露纳米颗粒的内在异质性方面具有独特优势.然而瑞利散射强度随粒径减小呈六次方衰减,使得小尺寸单个纳米颗粒的检测非常具有挑战性.本文对近年发展起来的多种单个纳米颗粒的光散射检测技术进行综述

李永康膏滋对小鼠胸腺端粒酶活性等免疫功能的影响

通过臭氧(O_3)致小鼠衰老模型,对各组抗衰老实验小鼠的生理和免疫功能进行检测,采用端粒重复序列分析(TRAP)方法检测衰老小鼠胸腺组织的端粒酶活性。李永康膏滋能使衰老小鼠爬绳、游泳和抗冻能力增强(P<0.05或P<0.01),血清总IgG浓度提高(P<0.01),脚掌发疱增重明显(P<0.01),胸腺指数增加(P<0.05或P<0.01);并使衰老小鼠胸腺端粒酶活性增强。表明李氏膏滋有延缓臭氧致小鼠衰老的作用,提高衰老小鼠体液和细胞免疫功能。使衰老小鼠胸腺端粒酶活性增强可能与该器官富含活化的淋巴细胞有关。教育部厦门大学细胞生物学和肿瘤细胞工程重点实验室开放研究基

Beam transformation in laser processing

激光光束形状和能量分布直接限定了激光加工的应用。为满足不同的激光加工要求,必须对激光光束进行光束变换。应用旋转棱镜和组合光学系统能够实现实心和环状光束之间的相互变换。从几何光学角度对旋转棱镜组合光学系统进行了理论分析和实验。通过调整正负旋转棱镜的间距d,获取不同形状和能量分布的激光光束。实验结果表明,基于旋转棱镜组合光学系统的光束变换技术,有效地实现了多种形式的光束变换,提高了激光束的利用率,在激光加工领域中具有广泛的应用前景。The shape and quality of laser beam directly define its applications in the laser processing.For different requests of laser processing,the input beam always needs to be transformed.The transformation between the solid beam and annular beam can be realized by the axicon-based optical devices.The optical system is analyzed based on the geometry optical theory.By adjusting the separation of the positive and negative axicon,different shapes and the energy distributions are gained.It is shown that the axicon-based beam transformations raise the use factor of laser and have wide application prospect in laser processing field.福建省科学技术厅资助项目(2005HZ1020

Current State and Prospect of Nanobacteria Research

探讨纳米细菌的分离培养、形态特征、极端环境适应性,生物矿化特性,致病性等方面的研究进展,指出目前纳米细菌研究存在的问题.提出应整合现代微生物学技术、分子生物学技术、纳米生物技术等多种研究方法,通过对纳米细菌的研究,力求在海洋环境、极地环境及极端生态环境中发现具有嗜酸、嗜盐、嗜热、抗高压等特殊生理生化特性,以及具有降解多环芳香烃、农药等特殊生态功能的菌种资源.This paper reviewed the isolation,morphological characters,flexibility to extreme environments,biomineralization properties,pathogenicity,existing problems in research of nanobacteria,pointed out that the existence of nanobacteria research should be made to integrate modern technology in microbiology,molecular biology technology,biotechnology,nanotechnology and other research method.Through the study of nanobacteria,we try to find acidophilus,halophilic,anopheles heat,high pressure,such as special anti-physiological and biochemical characteristics,as well as degradation of polycyclic aromatic hydrocarbons,pesticides and other special ecological functions of the strain resources from the marine environment,polar environment and the extreme ecological environments.福建省青年科技人才创新项目(2007F3094);; 厦门大学引进人才科研启动项目(0000-X071C3);; 厦门大学国家重点实验室开放研究基金资助项目(MEL0603);; 厦门大学教育部重点实验室访问学者研究基金资助项目(2007110);; 国家海洋局重点实验室开放研究基金资助项目(HY200601,200702

Measurement Model and Tip Clearance′s Spatial Filtering Effect of Eddy Displacement Sensor

基于电涡流位移传感器测量原理,建立叶尖间隙的有限元模型.根据涡轮发动机等旋转叶片设备叶尖间隙的结构特征与测量需要,建立了具有矢量特性的叶片点阵模型.分析叶片厚度、叶片转速、传感器敏感区大小、信号采样速率引起的空间滤波效应,以及对叶尖间隙测量结果的影响.研究结果表明,一定叶片厚度情况下,叶片转速、传感器敏感区、信号采样速率存在最低要求,这一结论可为叶尖间隙测量系统设计提供重要理论依据.The tip clearance measurement model is established by measurement principle of the eddy displacement sensor.According to structural characteristics and measurement needs of tip clearance of turbine engine and other rotating blade device,a leaf lattice model with vector characteristics is established.The effect of spatial filtering and the value of tip clearance measurement,caused by blade thickness,sensing zone size,blade rotation speed and signal sampling rate has been analyzed.The result shows that there is a minimum requirement about sensing zone size,blade rotation speed and signal sampling rate on the condition of certain blade thickness.The conclusion provides an important theoretical basis for the design of the tip clearance measurement system.国家自然科学基金(61172046

Relationship of Hp VacA Gene PCR Pattern to Gastroduodenal Disorders

目的　探讨幽门螺杆菌 (Hp)空泡毒素基因的两种亚型 vac A1 和 vac A2 与上消化道疾病的关系。　方法　采用特异性引物扩增 Hp尿素酶基因及 vac A基因。　结果　 6 6 .4% (81/ 12 2 )的患者胃粘膜检测出 Hp尿素酶基因 ,其中消化性溃疡患者的检出率 (74.6 % 5 3/ 71)高于慢性胃炎患者 (5 4.9% 2 8/ 5 1) ,二者差异有显著性。 vac A的两种亚型 vac A1 和 vac A2 各占 80 .2 % (6 5 / 81)和 19.8% (16 / 81) ,每种胃粘膜只能扩增出一种产物 ,其中 80 .7%(4 7/ 5 3)的消化性溃疡患者感染的是 vac A1 基因型 Hp,而慢性胃炎患者的检出率是 6 4.3% (18/ 2 8) ,差异有显著性。　结论　 vac A1 基因型 Hp与消化性溃疡关系密切。Objective\ To study two sub\|genotypes of vacA (vacA\-1 & vacA\-2) gene of Helicobacter pylori(Hp) and their relation with disease.\ Methods\ PCR was used to determine urease gene and vacA gene in Hp.\ Results\ Urease gene was found in 81(66 4%) of 122 case of Helicobacter pylori(Hp) infected patients.\ 74 6%(53/71) patients with peptic ulcer contained urease gene, which was significantly higher than those with chronic gastritis(54 9%,28/51).\ Only one sub\|genotype of vacA was detected in each specimen.\ In 81 urease gene positive cases, the positive rate of vacA\-1 gene was 80 2%(65/81), and that of vacA\-2\|gene was 19 8%(16/81).\ In 65 vacA\-1 positive cases, it was found in 80.7%(47/53) of peptic ulcer patients, and in 64.3%(18/28) of chronic gastritis.\ χ\+2 test showed that the positive rate of Hp with vacA\-1 gene in peptic ulcer differed significantly from that in chronic gastritis.\ Conclusion\ Hp with vacA\-1 gene is strongly related to peptic ulcer.\

长毛对虾酸性磷酸酶的纯化与性质

分别从健康和患病长毛对虾（Ｐｅｎａｅｕｓｐｅｎｉｃｉｌａｔｕｓ）肌肉提取一种酸性磷酸酶（ＡＣ－Ｐａｅ，ＥＣ．３．１．３．２．），进一步用硫酸铵分级分离，ＳｅｐｈａｄｅｘＧ－２００柱纯化，经聚丙烯酰胺凝胶电泳，并于ＢｅｃｋｍａｎＤＵ－８Ｂ紫外分光光度计扫描为单一区带酶液。该酶紫外吸峰在２８０ｎｍ处，荧光激发峰在２８４ｎｍ处，发射峰在３４７ｎｍ处。酶的分子量为７３０００道尔顿，等电点为４．７酶水解对硝基苯磷酸二钠（ＰＵＰＰ）最适ｐＨ为４．５，最适温度４０℃。健康虾与病虾酶的活化能分别为４１．０３ｋＪ／ｍｏｌ·Ｌ和４５．７８ｋＪ／ｍｏｌ·Ｌ，米多常数ｋｍ值分别为０．８０×１０－４ｍｏｌ／Ｌ和１．４３×１０－４ｍｏｌ／Ｌ。重金属离子Ｃｕ２＋，Ｈｇ２＋，有机溶剂甲醇，乙醇，乙二醇等对ＡＣＰａｓｅ有明显的抑制作用

混合蛋白质体系中魟鱼和海兔肝铁蛋白释放铁的动力学研究

分别制备含有魟鱼肝铁蛋白(liver ferritin of Dasyatisakajei, DALF)和海兔肝铁蛋白(Liver ferritin of Aplysia, ALF)的混合蛋白质体系。选用电子光谱技术和不同电子供体,研究在混合蛋白质体系中,DALF和ALF释放铁的动力学过程和规律。实验结果表明,采用Na2S2O4作为还原剂时,DALF以两相行为进行释放铁的反应;而选用抗坏血酸作为还原剂时,DALF却以一级反应动力学方式进行释放铁的反应,简化释放铁的过程。在混合蛋白质体系中且以抗坏血酸和Na2S2O4为电子供体时,ALF均以一级反应动力学过程进行释放铁的反应,认为某些蛋白质参与协助ALF释放铁反应,从而简化释放铁的过程

修形齿轮弯曲疲劳寿命对比试验研究

为探究修形参数对齿轮疲劳特性的影响,采用罗卡提法进行齿轮疲劳特性对比试验。试验采用错齿啮合方案,利用Romax确定了试验载荷相应的弯曲应力,并用Matlab对试验数据进行了处理分析,得到不同修形参数下的齿轮弯曲疲劳强度。试验结果表明,在给定工况下,选择合适的齿顶修形能够提高齿轮的弯曲疲劳寿命,为修形方法在齿轮中的应用提供了基础数据参考

The Relationship of Hp with cagA/vacA Gene to Gastroduodenal Disorders

采用 PCR法对 77例胃、十二指肠疾病患者胃粘膜 Hp cag A基因和 vac A基因的扩增以探讨cag A基因和 vac A基因型 Hp与上消化道疾病的关系 .77例 Hp感染患者中 ,60例检测出 cag A基因 ,阳性率为 77.9% ,消化性溃疡和慢性胃炎的阳性率分别为 87.8% (43/49)和 60 .7% (1 7/2 8) ,其差别有显著意义 (p<0 .0 1 ) .vac A的 2种亚型 vac A1 和 vac A2 各占 79.2 % (61 /77)和 2 0 .8% (1 6/77) .每种胃粘膜只能扩增出 vac A1 或 vac A2 的一种产物 ,其中 vac A1 在消化性溃疡和慢性胃炎中的检出率为 87.8% (43/49)和 64.3% (1 8/2 8) ,两者差别有显著性 (p<0 .0 2 ) .这些结果提示 cag A基因和 vac A1 基因型 Hp与消化性溃疡关系密切 ,这对 Hp相关的消化道疾病的预后和临床治疗有一定的指导作用To study the relationship of Hp with cagA/vacA gene to the gastroduodenal disorders, PCR was used to determine the cagA/vacA genotype of Hp in 77 cases of patients with gastroduodenal disorders. CagA gene was found in 60(77.9%) of 77 cases of Hp infected patients, including peptic ulcer(43/49, 87.8%) and chronic gastritis (17/28, 60.7%). According to x 2 test, significant difference of positive rate of Hp cagA gene existed among peptic ulcer and chronic gastritis. All Hp(77/77, 100%) carried vacA gene. Either, not both, of the two subgenotypes of vacA(vacA 1 &vacA 2) was detected in an individual specimen. In the 77 cases, the positive tate of vacA 1 gene was 79.2%(61/77) and that of vacA 2 gene was 20.8%(16/77). In the 61 cases with vacA 1 gene, vacA 1 gene was detected in patients of peptic ulcer (43/49, 87.8% ), and chronic gastritis(18/28, 64.3%). x 2 test showed that the positive rate of Hp with vacA 1 gene in peptic ulcer differed significantly from that in chronic gastritis. The results indicate that Hp with cagA/vacA 1 gene is strongly related to gastroduodenal disorders, which is meaningful to prognosis and therapy of gastroduodenal disorders infected by Hp