Synthesis and bio-application of Au, Ag nanocomposite based on Fe3O4

复合纳米材料由于其兼具多种纳米材料的性质和功能，能够克服单一纳米材料生物相容性差、易团聚、可修饰性弱等缺点，具有复合协同潜力，而成为纳米生物医学材料研究领域中最为重要的方向之一。在这其中，金、银和氧化铁的复合纳米颗粒由于兼具超顺磁性氧化铁纳米颗粒和贵金属纳米颗粒在磁学、光学、生物相容性、化学稳定性、表面可修饰性等多方面上的优异性能，因而在多功能成像、生物检测、传感、药物载体、疾病的早期诊断和治疗等生物医学方向上具有广阔的应用前景。目前，设计与合成具有多种不同功能的金、银和氧化铁的复合纳米颗粒和对其在生物体内的综合应用是纳米生物医学研究方向的热点之一。在本论文中，我们设计合成了多种具有不同结构的...Since nanocomposites has multifunction and many properties that come from various kinds of nanomaterials, they can overcome many defects of mono-nanomaterial, such as poor biocompatibility, easily aggregation and weakly surface paintability. The composite nanoparticles of Au, Ag and iron oxide have shown a lot of excellent performance in magnetism, optics, biocompatibility, chemical stability, pa...学位：理学博士院系专业：材料学院_材料物理与化学学号：2072013015363

与子女关系对老年人养老意愿的影响

目的了解与子女代际关系对老年人养老意愿的影响。方法采用多阶段抽样方法对老年人进行面对面问卷调查,内容包括社会人口学特征、家庭情况、健康状况和养老意愿等。采用Logistic回归分析与子女的关系对老年人养老意愿的影响。共完成1 292份问卷,有效1 259份,其中家庭养老、社区居家养老和机构养老的老年人分别占70.1%、21.0%和8.9%。结果以家庭养老为对照,与子女关系疏远者选择社区居家养老和机构养老的OR值(95%CI)分别为1.93(1.34～2.79)、2.68(1.61～4.47)。以机构养老为对照,与子女关系疏远者选择社区居家养老的OR值(95%CI)为0.72(0.42～1.25)。结论老年人多选择家庭养老,与子女的关系对其养老意愿有影响。厦门市老龄委委托项目(XDHT2013357A

Infection Status and Influencing Factors of Human Papillomavirus in Patients with Condylomata Acuminata

目的了解我国内地尖锐湿疣（CA）患者的人乳头瘤病毒（HPV）感染状况,并探讨其影响因素。方法系统检索Pub Med、Science Direct Online、中国知网、万方数据知识服务平台、维普网中关于我国内地CA患者HPV检测的文献,检索时间均为1990年1月—2013年12月。根据样本量对各文献HPV感染率进行加权合并,计算CA患者的HPV及各基因型合并感染率,并分析不同研究年份、研究地区CA患者的HPV合并感染率,采用多因素Logistic回归分析探讨CA患者感染/检出HPV的影响因素。结果共纳入文献67篇,累计CA患者10 757例,感染HPV 9 328例（86.7%）。CA患者的HPV合并感染率为95.7%〔95%CI（95.2%,96.2%）〕,其中合并感染率较高的前3种基因型为HPV6/11〔81.2%,95%CI（77.1%,85.2%）〕、HPV6〔43.8%,95%CI（35.2%,52.5%）〕、HPV11〔38.3%,95%CI（32.8%,43.8%）〕。1990—2003、2004—2013年CA患者的HPV合并感染率分别为96.6%〔95%CI（95.8%,97.5%）〕、94.6%〔95%CI（94.0%,95.3%）〕;东、中、西部患者的HPV合并感染率分别为95.1%〔95%CI（94.4%,95.7%）〕、91.7%〔95%CI（89.3%,94.1%）〕、96.5%〔95%CI（95.4%,97.6%）〕。多因素Logistic回归分析结果显示,研究年份、研究地区、标本类型、诊断方法是CA患者感染/检出HPV的影响因素（P〈0.05）。对不同基因型进行分析,结果显示,研究年份、研究地区、标本类型、诊断方法是CA患者感染/检出HPV6的影响因素,研究年份、研究地区为西部、标本类型、诊断方法是CA患者感染/检出HPV11的影响因素,研究地区为中部、标本类型是CA患者感染/检出HPV16的影响因素,研究地区、标本类型、诊断方法是CA患者感染/检出HPV18的影响因素（P〈0.05）。结论我国内地CA患者的HPV感染以HPV6、HPV11基因型为主,感染率近10年来有所下降,东部地区感染率较高。病理检查确诊、采用剥落细胞进行DNA检测,可以提高CA患者的HPV检出率。Objective To study the infection status of human papillomavirus（HPV） in patients with condylomata acuminata（CA） in mainland China and explore its influencing factors. Methods The literatures on HPV detection of CA patients in mainland China published between January 1990 and December 2013 were systematically retrieved from Pub Med,Science Direct Online, China National Knowledge Infrastructure, Wanfang Data Knowledge Service Platform, and the VIP database. According to the sample size,HPV infection rates of each literature were weighted and combined and the infection rates of HPV and the combined infection rate of each genotype of CA patients were calculated,and the combined infection rate of HPV of CA patients in different years and areas of study was analyzed. Multivariate Logistic regression analysis was used to investigate the influencing factors of CA patients infected / detected with HPV. Results A total of 67 literatures were included in the study. The number of CA patients accumulated to 10 757,and 9 328 cases（86. 7%） infected with HPV. The combined infection rate of HPV in patients with CA was 95. 7% 〔95% CI（95. 2%,96. 2%） 〕,and the top three genotypes with high infection rate were HPV6 /11 〔81. 2%,95% CI（77. 1%,85. 2%） 〕, HPV6 〔43. 8%,95% CI（35. 2%,52. 5%） 〕, HPV11〔38. 3%,95% CI（32. 8%,43. 8%） 〕. The combined infection rate of HPV in patients with CA between 1990 and 2003 and between 2004 and 2013 was 96. 6% 〔95% CI（95. 8%,97. 5%） 〕and 94. 6% 〔95% CI（94. 0%,95. 3%） 〕respectively;the combined infection rate of HPV of patients in eastern, middle and western areas was 95. 1% 〔95% CI（94. 4%,95. 7%） 〕,91. 7% 〔95% CI（89. 3%,94. 1%） 〕,and 96. 5% 〔95% CI（95. 4%,97. 6%） 〕respectively. Multivariate Logistic regression analysis showed that the year of study,areas of study,type of specimen and methods of diagnosis significantly influenced the infection and detection of HPV in CA patients（P 0. 05）. After analyzing different genoty福建省自然科学基金青年项目（2014J05097

基于递归系统模型的宫颈癌患者住院费用影响因素分析

目的分析宫颈癌患者住院费用的影响因素,为控制住院费用提供依据。方法收集厦门市某三甲医院2012 -; 2014年入院接受治疗且信息完整的宫颈癌患者的一般情况和住院费用等资料,通过递归系统模型分析各因素的直接与间接效应,计算总效应,找出费用控制的关; 键性因素。结果共纳入524例宫颈癌患者,其中医保患者占49.8%,首诊年龄的中位数为48岁,住院天数的中位数为28天,总费用的中位数为43841; .06元。支付方式、转移情况对总费用仅有直接效应,同时治疗其他疾病、MRI检查仅有间接效应;; 临床分期、治疗方案、手术方式既有直接效应又有间接效应;; 治疗方案的总效应值最大。结论宫颈癌住院费用受到多个因素的直接效应、间接效应、直接和间接效应的影响,其中以治疗方案的影响最大。可通过规范化制定治疗; 方案控制宫颈癌的住院费用。厦门大学大学生创新项目基

细颗粒物电凝并技术研究进展

雾霾的主要污染物是细颗粒物PM2.5,主要来自燃煤过程,传统除尘技术对PM2.5的捕获效率较低,通过电凝并技术使PM2.5凝聚成大颗粒,再通过传统除尘器脱除,可提高PM2.5的捕集效率。综述了国内外电凝并技术的研究进展,主要包括静电场中异极性荷电颗粒凝并、交变电场中同极性荷电颗粒凝并、交变电场中异极性荷电颗粒凝并;介绍了一种新型荷电凝并装置,细颗粒物与大颗粒之间运动与凝并的直接观测实验,为电凝并技术的实际应用和装备开发提供技术支撑。</p

PROPOSED SINGLE STEP TWO-ELECTRON REDUCTION OF PRONTON REDUCTION MOLECULAR CATALYST IN CoPy/CdS PHOTOCATALYTIC SYSTEM

PROPOSED SINGLE STEP TWO-ELECTRON REDUCTION OF PRONTON REDUCTION MOLECULAR CATALYST IN CoPy/CdS PHOTOCATALYTIC SYSTE

A Peptidoglycan Recognition Protein (PGRP) from Haliotis discus hannai: Possible Roles in Antibacterial Properties

In this study, peptide PGRP (designated HdPGRP) was identified and characterized from the abalone Haliotis discus hannai. Multiple alignments and phylogenetic analyses strongly suggested that HdPGRP is a new member of the PGRP superfamily and belongs to the short PGRP family, similar to peptides from other marine mollusks. The full length of HdPGRP is 1467 bp, encoding a polypeptide of 354 amino acids (aa) with a signal peptide (1~18 aa), an SH3b domain (93~160 aa), a typical PGRP domain (179~322 aa), and an Ami_2 domain (191~322 aa). In addition, four conserved Zn~(2+)-binding sites (H~(209), Y~(255), H~(318), and C~(330)) and five conserved amide-catalysis sites (H~(209), Y~(255), H~(318), T328, and C~(330)) were found in the HdPGRP sequence. In abalone, hdpgrp exhibited different tissue expression patterns, and was strongly expressed in the hepatopancreas, moderately expressed in hemocytes, mantle, and gills, and slightly expressed in muscle. Vibrio anguillarum is one of the main pathogens of H. discus hannai; after V. anguillarum infection, expression of hdpgrp in hemocytes showed a trend of first increasing and then decreasing, reaching a maximum at 24 h. Subsequently, expression of HdPGRP decreased, and there was no significant difference compared with the control group at 72 h, demonstrating that expression of HdPGRP had returned to normal levels. SDS-PAGE results showed that recombinant HdPGRP (rHdPGRP) has a molecular mass of 30 kDa, which is in line with the value predicted for HdPGRP. PGRPs usually have amidase activity, degrading peptidoglycan by hydrolyzing the amide bond that links peptide units to muramic acid residues of glycan strands. rHdPGRP exhibited Zn~(2+)-dependent amidase activity and catalyzed the degradation of insoluble peptidoglycan. In addition, rHdPGRP exhibited significant antibacterial activity against the gram-positive bacterium Micrococcus luteus in the logarithmic phase in the presence of Zn~(2+), indicating that the antibacterial activity of HdPGRP might be dependent on amidase activity. In summary, HdPGRP plays an important role in PGRP-mediated antibacterial mechanisms, especially for eliminating invading bacteria

混合样品中微量SRY基因的毛细管电泳检测

建立一种灵敏度高，重现性好的检测混合样品中微量目的基因的方法。方法采用聚合酶链反应扩增混合样品中微量男性ＤＮＡ的ＳＲＹ序列，分别通过琼脂糖凝胶电泳和毛细管电泳检测ＰＣＲ扩增产物，比较二者的检测灵敏度