The Change of The Minnan Dialect Variety Show and Political Culture inTaiwan

随着台湾综艺节目在大陆走红，台湾综艺节目已成为大陆年轻人了解台湾的重要窗口之一。台湾闽南语综艺节目是台湾综艺节目的一个分支，它以闽南语为主要播出语言，重在表现台湾的本土俚俗语言和民俗文化，充满了浓厚的台湾本土特色。 语言制裁是台湾当局巩固政权、控制意识形态的主要渠道，政治势力严密地控制着社会的各个层次方面，甚至渗透到了通常是为电视台牟利的综艺节目。长期以来，台湾闽南语综艺节目的生存发展不仅受台湾经济力起伏的影响，还与台湾政治的变迁紧密地交织在一起，当局对闽南语的戒严或松绑都直接影响着闽南语综艺节目的命运。闽南语综艺节目的发展脱离了电视本身的规律，而通过商业电视的控制，被纳入到了政治策略的轨道...As the prevalence of Taiwan variety show, they have become the important windows for the youth in China mainland to get to know Taiwan. Particularly, The Minnan dialect variety show are one branch of the whole package, using the Minnan dialect as the main language, centering on expressions of local language and folk culture, featuring strong local atmosphere. Language sanction is the primary meth...学位：文学硕士院系专业：人文学院中文系_戏剧戏曲学学号：1022008115245

Research on Moving Target Detection and Tracking Method

目标的监测与追踪是智能视频系统研究的重要内容，在科技、军事、交通、 安全、医疗等各个方面都发挥了举足轻重的作用。移动对象的监测与追踪是智能 视频系统中的设计中枢，是视觉研究的根本，因此做好目标监测追踪研究很有必 要。本文以是静态背景下的移动目标监测追踪为研究对象，主要做了如下研究工 作： 在目标监测方面，梳理了目标图像识别最经典的三种算法：光流法、背景差 分法和帧间差分法，并重点研究结合了背景差分法、最大投影区域与三帧差分法 三种方法的混合差分法，提高了系统监测的准确性和实时性。 在目标追踪方面，在综述了移动目标追踪技术的基本研究概况的基础上，本 部份主要讨论了均值漂移目标追...Target detection and tracking play a very important role in the field of Intelligent video system,which has been broadly applied in scientific research, transportation, safety and health area．The design of Moving target detection and tracking method is the essence of in the intelligent video system．Therefore,do a good job in target detection and tracking research is still very meaningful．Based...学位：工程硕士院系专业：信息科学与技术学院_计算机技术学号：X200822101

对话第十一届中国戏剧节

中国戏剧节始于1988年,它是由中国文学艺术界联合会、中国戏剧家协会共同主办的全国性大型戏剧演出活动,两年一届。第十一届中国戏剧节于2009年底在福建省厦门市举办。本届戏剧节已落幕多日,众声喧哗亦渐消歇。本刊特登两篇来自厦门大学戏剧戏曲学专业师生的讨论文章,以窥此次戏剧节演出之一孔

Determination of Chondroitin Sulfate with High Sensitivity by Fluorescence Recovery through Shifting the Equilibrium between Anionic Phthalocyanine and Cationic Surfactant

目的建立高灵敏荧光增强法测定硫酸软骨素的新方法,并用于实际样品测定。方法对荧光光谱行为的考察显示,在PH 7.0的介质中,具有共轭结构的阳离子头部和长碳链尾部的阳离子表面活性剂对四磺基铝酞菁(AlS4PC)的荧光具有高效猝灭作用,二者形成无荧光离子缔合物。此离子缔合物可对带有磺酸基团的高分子黏多糖硫酸软骨素(CS)作出灵敏响应。在CS的存在下,反应体系荧光显著恢复,最大恢复倍数可达900倍。由于猝灭效率高,荧光恢复程度大,甚至可实现目视化观测。结果工作曲线响应区间0.2~6μg·Ml-1,检测限0.09μg·Ml-1,工作曲线lOgy=0.158 23ρ-0.035 97,r=0.998 7。本实验对该法用于批量样品分析的可行性进行了考察,结果表明,所建立的方法可望用于高通量分析。结论该法操作简便,灵敏度、稳定性与检测准确性好。所建立的方法成功地用于组成复杂的实际样品中CS的定量分析,解决了实际工作中的难点。OBJECTIVE To propose a novel method for quantitative determination of chondroitin sulfate in real samples using the ion-association phthalocyanine complex as a fluorescent probe emitting at red-region.METHODS The fluorescence of tetrasulpnonated aluminum phthalocyanine( AlS4Pc),an anionic metal phthalocyanine,was quenched dramatically by cationic surfactants which contain a positively-charged head with a conjugated structure and a long carbon chain as tail through the formation of an almost non-fluorescent association complex.Hexadecylpyridinium bromide( CPB) screened from a series of cationic surfactants was selected as a quencher because of its high fluorescence quenching efficiency.It was found that the ion-association complex( AlS4Pc-CPB) emitted strong fluorescence in the presence of chondroitin sulfate,due to the ability of chondroitin sulfate to shift the association equilibrium of the ionassociation complex,which led to the release of AlS4Pc,thus resulting in an increase in the fluorescence of AlS4Pc.The method based on the above-mentioned phenomenon was investigated in the aspects of spectral characteristics,effect of pH,influence of reaction time, order of addition of reagents,the usage of reagents,and interference of foreign substances.RESULTS Under optimal conditions,the linear range of the assay for chondroitin sulfate was 0.2- 6 μg·mL- 1with a detection limit of 0.09 μg·mL- 1.CONCLUSOIN The established method is not only sensitive,accurate but also simple and reliable.It has been used to the analysis of real samples with complicated composition with satisfactory results.It has also been successfully applied in batch test combined with well-reader technique,exhibiting great potential for high-throughput analysis.福建省科技重点资助项目(2012Y0081); 福建省公益类科研院所基本专项(2010R1101)资助项

Novel Method for Determination of Lysozyme Based on Fluorescence Recovery of a Cationic Aluminum Phthalocyanine-Mucopolysaccharides Association Complex Used as a Red Emitting Fluorogenic Substrate

在弱酸性介质中,具有红区发射特性的强荧光化合物阳离子铝酞菁(TTMAAlPC)在带磺酸基团的低浓度阴离子黏多糖(肝素,HP)的存在下,发生诱导聚集,导致酞菁荧光几乎完全猝灭。此聚集缔合物可作为溶菌酶的荧光底物,在溶菌酶的水解作用下,HP降解为小分子片段,破坏了TTMAAlPC的诱导聚集行为而使其释放,体系荧光显著恢复。据此现象,建立了测定溶菌酶的新方法。结合荧光光谱与荧光各向异性技术对反应机理进行了探讨。确定了最佳反应条件(醋酸缓冲体系,PH4.0、反应温度70$C、反应时间30 MIn),考察了共存物质的影响。在最佳条件下,方法的线性回归方程为y=#30.12121+214.65772X,r=0.99871,线性范围为0.2~2 Mg/l,检出限0.015 Mg/l。本研究操作简便且有较好的选择性和灵敏度。本方法用于溶菌酶实际样品的测定,并与常规的比浊法进行了比较,结果符合良好。本研究将阳离子金属酞菁荧光探针用于酶分析,开拓了酞菁荧光探针的应用范围。We developed a novel method for the rapid determination of lysozyme using a new fluorogenic substrate that consists of a cationic aluminum phthalocyanine( tetra( trimethylammonio) aluminum phthalocyanine,TTMAAlPc), and an anionic mucopolysaccharide( heparin, HP).We found that fluorescence from the cationic aluminum phthalocyanine,a red-region fluorescence probe,was quenched significantly in acidic media in the presence of low concentrations of anionic mucopolysaccharide heparin( HP) bearing anionic sulfonic acid groups,because of induced aggregation.The practically non-fluorescent substrate degraded into small molecular fragments upon the hydrolysis of lysozyme, and thus the phthalocyanine molecules aggregated in HP were released,resulting in significant fluorescence recovery in the reaction system.This phenomenon forms the foundation of the proposed method.The reaction mechanism was determined using fluorescence spectroscopy and fluorescence anisotropy techniques.Factors that affected the determination were investigated.Under optimal conditions,the linear range was 0.2- 2 mg /L,and the detection limit was 0.015 mg /L.The developed method is easy to operate and has good selectivity and sensitivity.This method was used in the analysis of practical samples of lysozyme,and the results were in agreement with those determined by a conventional turbidimetric method.国家自然科学基金重大研究计划项目(No.90206016); 福建省科技重点项目(No.2012Y0081); 福建省属公益类科研院所基本科研专项(No.2010R1039-3)~

Sensitive Determination of Chondroitin Sulfate by Fluorescence Recovery of an Anionic Aluminum Phthalocyanine-Cationic Surfactant Ion-Association Complex Used as a Fluorescent Probe Emitting at Red Region

硫酸软骨素的测定在生物医学领域有重要价值,但常规检测法在灵敏度、选择性或简易性方面尚存在不足。本文基于带正电基团的阳离子表面活性剂对具有红区发射特性的强荧光化合物—荷负电的四磺基铝酞菁具有高效荧光猝灭作用,而在生物多糖硫酸软骨素存在下,上述荧光猝灭体系荧光显著恢复的现象,提出酞菁-表面活性剂离子缔合物荧光恢复高灵敏测定硫酸软骨素的新方法,并用于实际样品分析。研究表明,中性介质中,红区荧光探针四磺基铝酞菁(TETrASulfOnATEd AluMInIuM PHTHAlOCyAnInE,AlS4PC)与阳离子表面活性剂十四烷基二甲基苄基氯化铵(TETrAdECyldIMETHylbEnzylAMMOnIuM CHlOrIdE,TdbAC)发生强烈的缔合作用,导致AlS4PC的荧光几乎完全猝灭,从而获得暗背景的荧光体系。在加入带有阴离子基团(磺酸基)的生物多糖硫酸软骨素(CHOndrOITIn SulfATE,CS)后,由于竞争结合作用,AlS4PC被释放而使体系的荧光大幅度恢复,且恢复程度与CS呈线性正相关。优化了反应条件,考察了共存物质的影响,结果表明本法具有较好的选择性。在最佳条件下,线性范围为0.20~10.0μg·Ml-1,检测限为0.070μg·Ml-1,工作曲线方程y=1.04X+2.09,r=0.999 5。该法操作简便,灵敏度、稳定性与准确性好,实际样品的分析结果令人满意。酞菁荧光化合物在分析科学中的应用尚不多见,本文工作进一步开拓了酞菁红区荧光探针的新应用。Determination of chondroitin sulfate in the biomedical field has an important value.The conventional methods for the assay of chondroitin sulfate are still unsatisfactory in sensitivity,selectivity or simplicity.This work aimed at developing a novel method for sensitive and selective determination of chondroitin sulfate by fluorimetry.We found that some kinds of cationic surfactants have the ability to quench the fluorescence of tetrasulfonated aluminum phthalocyanine(AlS4Pc),a strongly fluorescent compound which emits at red region,with high efficiency.But,the fluorescence of the above-mentioned fluorescence quenching system recovered significantly when chondroitin sulfate(CS)exits.Tetradecyl dimethyl benzyl ammonium chloride(TDBAC)which was screened from all of the candidates of cationic surfactants was chosen as the quencher because it shows the most efficient quenching effect.It was found that the fluorescence of AlS4 Pc was extremely quenched by TDBAC because of the formation of association complex between AlS4 Pc and TDBAC.Fluorescence of the association complex recovered dramatically after the addition of chondroitin sulfate(CS)due to the ability of chondroitin sulfate to shift the association equilibrium of the association,leading to the release of AlS4 Pc,thus resulting in an increase in the fluorescence of the reaction system.Based on this phenomenon,a novel method with simplicity,accuracy and sensitivity was developed for quantitative determination of CS.Factors including the reaction time,influencing factors and the effect of coexisting substances were investigated and discussed.Under optimum conditions the linear range of the calibration curve was 0.20~10.0μg·mL-1.The detection limit for CS was 0.070μg·mL-1.The method has been applied to the analysis of practical samples with satisfied results.This work expands the applications of AlS4 Pc in biomedical area.福建省科技重点项目(2012Y0081); 福建省公益类科研院所基本专项项目(2010R1101;2014R1102); 厦门市科技项目(3502Z20143015)资

完善厦门居家养老、医养结合、智慧养老服务体系——面对“十三五”期间养老需求

目前．厦门市60周岁以上户籍老年人已达29．22万人．较2014年同期增长0．51万人，占全市总人口的14．05％．人口老龄化严重。虽然，在党和各级政府的高度重视下，厦门市的养老服务体系建设取得了一定的成果。在全国率先建立城乡一体的全民医保，城镇居家养老服务普遍开展，并逐步向农村延伸；在全省率先开通养老信息化平台暨12349养老服务热线并投入使用