Prokaryotic Expression, Antifreeze Activity Identification of Type Ⅲ AFP HPLC-12 and Preliminary Construction of the Transgenic Expression Vector of Haliotis diversicolor

摘要 杂色鲍（Haliotisdiversicolor）是我国南方重要的水产养殖种类，近年来由于诸多原因，其海产养殖业中的比例逐年下降。本文的研究目的即是以Ⅲ型AFPHPLC-12抗冻蛋白序列构建杂色鲍AFP转基因载体。希望利用此载体通过转基因的方法使杂色鲍获得耐低温、抗冻性状，快速培育杂色鲍新品种，向中国北部沿海方向扩大杂色鲍的养殖范围。 按照去除其前部的信号肽序列保留AFP功能区（261bp）的Ⅲ型AFPHPLC-12序列设计引物，合成Ⅲ型AFPHPLC-12基因序列，同时在片段两端分别增加BamHⅠ和SalⅠ酶切位点。测序结果表明克隆所得序列全长为275bp，去掉两端酶切位点后长度为...Abstract Small abalone, Haliotis diversicolor is an important aquaculture species in South China. In recent years, because of a series of reasons, the proportion of the small abalone in the aquaculture is decreasing year by year. The objective of this study is to construct the transgenic vector with the sequence fragment of the antifreeze protein type Ⅲ AFP HPLC-12. By the method of transgene,...学位：理学硕士院系专业：海洋与环境学院海洋学系_海洋生物学学号：2005130216

Reorder discrete cosine transform method for video compression

为了提高离散余弦变换(dCT)在视频图像编码中的效率,根据dCT对非水平/非垂直残差信号较敏感的特点,从改变残差信号分布的角度出发,提出一种重排dCT方法.对待编码的残差图像块进行像素位置重排,使得残差信号的方向与水平或垂直方向相一致;对重排后的图像块进行2维dCT,从而减少dCT系数的高频分量,提高压缩效率.实验结果表明:在不考虑重排信息的情况下,重排dCT比传统dCT性能提高0.20~0.30db;在考虑重排信息的情况下,重排dCT比传统dCT性能提高0.05~0.20db.In traditional video compression,discrete cosine transform (DCT) is sensitive to the prediction error with non-horizontal or non-vertical orientations.To improve the performance of DCT in video compression,this work proposed a reorder DCT method,from the view point of modifying signal distribution.In the algorithm,reorder operation was first used to change the pixel position of the blocks to be transformed,and then 2D-DCT was applied.Experimental results show that the reorder DCT can reduce the number of DCT coefficients at high frequency.Compared to the traditional DCT,the reorder DCT achieved 0.20-0.30 dB peak signal-to-noise ratio (PSNR) gain without reorder information.With reorder information,0.05-0.20 dB PSNR gain could be obtained.福建省科技厅重大专项资助项目(2007HZ0003

家族性淀粉样多发性神经病(3个家族13例患者,3例病理证实并文献复习)

目的　结合文献复习 ,探讨家族性淀粉样多发性神经病的发病机制和临床特征。方法　报道 3个台湾籍家系共 13例患者 ,对每个家系的先证者进行临床分析。结果　本组所有患者临床上均表现为植物神经症状 ,如排尿障碍、胃肠症状和阳痿等 ,体格检查提示为严重的感觉运动神经病、体位性低血压和消瘦。神经电生理检查提示周围神经和神经根轴索损害及脱髓鞘改变。病理学检查发现 3例先证者均有刚果红染色阳性的淀粉样物沉积于神经束间质及神经内膜中。结论　对于进行性发展的伴突出植物神经症状的慢性周围神经病 ,若有多脏器功能受损及阳性家族史 ,应考虑本病 ,病理检查有重要意

初诊为抑郁症的老年隐球菌性脑膜炎6例报告

目的:探讨老年隐球菌性脑膜炎发病特点和误诊原因。方法:分析6例初诊为抑郁症的老年隐球菌性脑膜炎患者的临床表现、脑脊液特点、治疗和预后表现,以明确发病特点和误诊原因。结果:6例病例初期均表现为头晕、失眠、食纳减少;后期出现颅内感染相对特异症状:认知障碍5例、头痛3例、肢体无力3例、发热2例。脑脊液隐球菌培养阳性明确诊断,抗真菌治疗有效。6例均好转,但是2例患者遗留明显后遗症:1例患者遗留明显的认知障碍和双下肢活动受限,1例双眼失明,2例确诊时间为2、4周。结论:老年患者患病后临床症状常不典型,当一种疾病不能解释所有症状时,需开拓思路,积极完善相关检查,必要时请相关科室协助诊治,尽量减少误诊。福建省厦门市科技局资助项目(项目编号：3502Z20084019

The Construction of Expression Vector pIRES2-EGFP-HdiActinP-AFP for Transgenic Small Abalone Haliotis diversicolor Using Antifreeze Peptide Ⅲ

现代转基因技术是一种快速改良品种特性的有效方法.采用转基因技术提高我国南方重要贝类经济养殖品种——杂色鲍的耐低温特性是扩大其养殖范围的重要手段.以PIrES2-EgfP为基本载体,以合成并经过功能鉴定的抗冻肽(AfP)和杂色鲍肌动蛋白启动子(HdIACTInP)为插入元件,首先用限制性内切酶bAMHⅠ和ECOrⅠ对基本载体PIrES2-EgfP和AfP-Ⅲ进行双酶切,目的酶切产物被回收后再连接得到载体PIrES2-EgfP-AfP.随后利用限制性内切酶SACⅠ和ECOrⅠ对载体PIrES2-EgfP-AfP和HdIACTInP进行双酶切,目的酶切产物被回收后再连接最终得到杂色鲍转AfP-Ⅲ的表达载体PIrES2-EgfP-AfP-HdIACTInP,经过聚合酶链式反应和测序验证证明载体构建成功.Small abalone,Haliotis diversicolor is one of important maricultural species which distributes in South China.Modern transgenic technique is a rapid and direct way to improve characterization of aquaculture species.It enables us to breed a new line of small abalone which has a low temperature-resistant feature so that it can be cultured in wider areas than now.In this study,pIRES2-EGFP was used as the basic vector,and the antifreeze peptide Ⅲ and HdiactinP were used as inserting elements.Firstly the vector pIRES2-EGFP and segment AFP were digested with the restriction endonuclease BamHⅠ and EcoRⅠ.The target components of the digested product were ligated consequently and the vector pIRES2-EGFP-AFP was constructed.Secondly the vector pIRES2-EGFP-AFP and segment HdiactinP were digested with restriction endonuclease SacⅠand EcoRⅠ.Then the target components of the digestion product were ligated.Finally the transgenic H.diversicolor antifreeze peptide expression vector pIRES2-EGFP-AFP-HdiActinP was constructed,which was confirmed by polymerase chain reaction(PCR)and sequencing.In summary,our result provided a potential basis for the consequent development of the transgenic H.diversicolor researches.国家863计划课题(2006AA10A407);农业科研专项(nyzx07-047);现代农业产业技术体系建设专项(nycytx-47)资

利用Hello消息改进AODV路由的实时性

为解决经典AODV（按需距离矢量）路由协议中目的节点在已建立路由链路信号覆盖范围内随机移动时脱离其原来直接通信节点而断路、通信恢复时间长以及网络负载大等问题,对利用AODV协议Hello消息报文激活中间节点与目的节点的通信切换实现快速路由修复进行了研究,并得到改进后的路由协议AODVL。仿真结果表明,AODVL比经典的AODV路由协议减小了网络负载并缩短了路由修复时间。AODVL更适用于目的节点在已建立路由链路信号覆盖范围内随机移动的网络,节点间通信具有更高的实时性

Cloning and Sequence Analysis of the Abalone H.diversicolor diversicolor's Actin Promoter

杂色鲍(H.diversicolor diversicolor)是我国华南地区重要的海水养殖对象,作为利用转基因技术改良杂色鲍品质研究工作的首要部分,作者利用PCR和染色体步移(Genome Walking)方法克隆了杂色鲍肌动蛋白基因编码区部分序列和启动子序列(GenBank登陆号:EU622901).所分离的肌动蛋白基因DNA序列片段全长为1 990 bp,与GenBank中彩虹鲍(Haliotis iris)肌动蛋白A1基因序列和肌动蛋白A1a基因序列有非常高的相似性(99%和96%).在基因编码区中间发现了一个内含子区,在编码区之前发现一个类启动子区,经过Promoter Prediction在线软件分析,发现了该启动子序列和转录起始位点,同时也发现了该启动子特有的启动元件两个标准的CAAT BOX和一个TATA BOX.这些实验结果表明所克隆的杂色鲍肌动蛋白和启动子序列符合实验的目的,并且这一实验的成功也为下一步利用所分离的启动子做杂色鲍转基因工作打下基础.H.diversicolor diversicolor(the small abalone) is the important aquaculture species in South China.As the primary part of the study in the transgenic small abalone,the sequence fragment of the Actin gene and its promoter was cloned and characterized by using the technique of PCR and Genome Walking(GenBank Accession No.EU622901).The length of the isolated sequence fragment of the small abalone Actin is 1990 bp,which has high similarity with the A1 gene and A1a gene(99% and 96%) of the Haliotis iris.A promoter-like sequence and an intron sequence before the Actin gene coding region was conjectured by sequence analysis.Starting elements,two CAAT BOXes and a TATA BOX,have also been identified by using online software Promoter Prediction.All these experimental results show that the sequence of the Actin gene we cloned from the small abalone is in according with the experiment purpose,and these results could be the foundation for transgenic operation.国家863计划课题(2006AA10A407)资

A Peripheral Edge Detection Method for Multiple Indexable Inserts

针对可转位刀片周边刃边缘检测的问题,提出了一种适用于多个可转位刀片的基于局部模糊检测和双树复小波变换的边缘检测算法。该算法主要分为两步,第一步是先按事先规定的要求拍摄可转位刀片的图片,然后对拍好的图片进行局部模糊检测处理,其中的检测算法是以图片中像素的相关性系数和基于Laplacian算子的新型判别算子为评价指标判断其是否位于模糊区域（若位于模糊区域,则用白色显示它,反之,则用黑色显示）。模糊检测算法在剔除误检像素后最后会生成一副二值图,可转位刀片的周边刃边缘即为二值图中黑色区域和白色区域的交界线。第二步就是用双树复小波变换对二值图进行边缘检测,检测后的结果就是我们需要的结果。实验结果证明,该算法能准确快速地检测出多个可转位刀片的周边刃边缘。As for the problem of peripheral edge detection for indexable inserts , this paper propose an algorithm of edge detection based on local-blur detection and dual-tree complex wavelet transform for multiple indexable in- serts. The algorithm have two steps： the first step is to take pictures of indexable inserts according to the require- ments specified in advance firstly,and then make local-blur detection in the picture which was been taken,here the algorithm of edge detection judge whether the pixel is in the blur area with the correlation coefficients of the pixel in the picture and the new operator based on laplaeian operator as evaluation indexs （if it is in the blur area, show it in white; otherwise, show it in black ）.After eliminating the mistake pixels, the last step of the algorithm of edge detection will create a binary image,the peripheral edge of indexable inserts are the demarcation lines be- tween white areas and black areas in the binary image.The second step is to detect the edges in binary image by using dual-tree complex wavelet transform,the result ater transform is that we need. Experimental results show that this method can detect the peripheral edge of multiple indexable inserts quickly and accurately.闽发改投资[2016]482

Effects of high salinity, high temperature and pH on capsid structure of white spot syndrome virus

National Basic Research Program of China (973 Program) [2012CB114401]; Natural Science Foundation of China [31072243]; China Agriculture Research System-47The structural stability of white spot syndrome virus (WSSV) capsids at high salinity, high temperature and various pH values was studied. To obtain the viral capsids, the nucleocapsids were treated with high salinity. The results showed that high salinity treatment can cause the dissociation of VP15 and most of VP95 from the nucleocapsid, but there were no noticeable alterations in morphology and ultrastructure of the nucleocapsid and capsid. The capsids retained morphological integrity at temperatures 60 degrees C. In addition, the capsids were relatively resistant to strong acid conditions and were tolerant to a broad pH range of 1 to 10. However, morphological change occurred at pH 10.5. The capsids broke up into small pieces at pH 11 and completely degraded in 0.1 and 1.0 M NaOH. These results indicated that the WSSV capsid is acid-stable and alkali-labile