杂色鲍(H.diversicolor diversicolor)是我国华南地区重要的海水养殖对象,作为利用转基因技术改良杂色鲍品质研究工作的首要部分,作者利用PCR和染色体步移(Genome Walking)方法克隆了杂色鲍肌动蛋白基因编码区部分序列和启动子序列(GenBank登陆号:EU622901).所分离的肌动蛋白基因DNA序列片段全长为1 990 bp,与GenBank中彩虹鲍(Haliotis iris)肌动蛋白A1基因序列和肌动蛋白A1a基因序列有非常高的相似性(99%和96%).在基因编码区中间发现了一个内含子区,在编码区之前发现一个类启动子区,经过Promoter Prediction在线软件分析,发现了该启动子序列和转录起始位点,同时也发现了该启动子特有的启动元件两个标准的CAAT BOX和一个TATA BOX.这些实验结果表明所克隆的杂色鲍肌动蛋白和启动子序列符合实验的目的,并且这一实验的成功也为下一步利用所分离的启动子做杂色鲍转基因工作打下基础.H.diversicolor diversicolor(the small abalone) is the important aquaculture species in South China.As the primary part of the study in the transgenic small abalone,the sequence fragment of the Actin gene and its promoter was cloned and characterized by using the technique of PCR and Genome Walking(GenBank Accession No.EU622901).The length of the isolated sequence fragment of the small abalone Actin is 1990 bp,which has high similarity with the A1 gene and A1a gene(99% and 96%) of the Haliotis iris.A promoter-like sequence and an intron sequence before the Actin gene coding region was conjectured by sequence analysis.Starting elements,two CAAT BOXes and a TATA BOX,have also been identified by using online software Promoter Prediction.All these experimental results show that the sequence of the Actin gene we cloned from the small abalone is in according with the experiment purpose,and these results could be the foundation for transgenic operation.国家863计划课题(2006AA10A407)资
