Effect of HJJB Compound on Insulin Signal Transduction Link of Non-alcoholic Steatohepatitis Rats

目的观察红景天苷、姜黄素、绞股蓝总苷、白术多糖(HJJB)复方对非酒精性脂肪性肝炎(NASH)大鼠胰岛素信号转导环节的干预作用。方法采用高脂饮食; 14周诱导的大鼠胰岛素抵抗NASH模型。在造模第9周起,随机分为模型组、西药组(罗格列酮,0.4; mg/kg)和中药组(HJJB)。干预6周后,观察肝组织病理变化(HE染色),检测肝组织TG含量、ALT活性、血清空腹胰岛素(FINS)含量、空; 腹血糖(FBG)含量、胰岛素抵抗指数(HOMA-IR),检测肝组织胰岛素受体底物1 (IRS1 )、磷酸化IRS1 (pIRS1; )、磷脂酰肌醇-3-激酶(PI3K)、磷酸化PI3K(pPI3K)、蛋白激酵B(PKB)、磷酸化PKB(pPKB)蛋白含量;检测肝组织IRS1、; PI3K、PKB mRNA水平。结果与正常组比较,模型组出现肝细胞脂肪变性, TG、ALT、FINS、FBG 及 HOMA-IR 升高(P; <0. 01), IRS1、plRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB mRNA降低(P; <0.01)。与模型组比较,中药组和西药组上述病理改变明显减轻,血清TG、ALT、 FINS、FBG 及 HOMA-IR; 含量降低(P<0.05)。中药组 IRS1、pIRS1、PI3K、pPI3K、PKB、pPKB 蛋白及 IRS1、 PI3K、PKB; mRNA水平较模型组及西药组升高(P <0.01,P <0.05),TG及ALT较西药组降低(P <0. 01; )。结论HJJB复方可上调NASH大鼠肝脏IRS1基因表达和蛋白含量,改善PI3K/PKB信号通路。Objective To observe the intervention effect of HJJB; compound(salidroside, curcumin, gypenosides and atractylodes; polysaccharides) on insulin signal transduction link of non-alcoholic; steatohepatitis (NASH) rats. Methods SD male rats were induced by; high-fat diet for 14 weeks for insulin resistance NASH model. From the; ninth week, the rats were divided into the model group, the Western; medicine(WM) group (rosiglitazone, 0. 4 mg/kg) and the Chinese medicine; (CM)group (HJJB) at random .Six weeks after medication, liver pathology; (HE staining), hepatic TG content, serum ALT activity, serum fasting; insulin (FINS), serum fasting blood glucose( FBG), insulin resistance; index (HOMA-IR) were observed. Protein content of hepatic insulin; receptor substrate insulin receptor substrate 1 (IRS1), phosphorylation; of IRS1 (pIRS1),phosphatidylinositol-3 kinase (PI3K) , phosphorylation; of PI3K(pPI3K), protein kinase B (PKB) and phosphorylation of PKB (pPKB); were detected. mRNA expression of hepatic IRS1,PI3K, PKB were also; detected. Results Significant hepatic steatosis were observed in the; model group. TG, ALT, FINS, FBG and HOMA-IR of model group were higher; than those of the normal group(P < 0. 01). Hepatic IRS1,pIRS1 ,; PI3K,pPI3K, PKB, pPKB protein expression level and IRS1 , PI3K,PKB mRNA; level were lower than those of the normal group (P <0. 01). Hepatic; pathological changes in the CM group and WB the group were meliorated,; ALT, FINS, FBG, HOMA-IR and TG of the CM group and the WM group were; lower than those of the model group(P <0. 05). Hepatic; IRS1,pIRS1,PI3K,pPI3K, PKB, pPKB protein expression level and IRS1,; PI3K, PKB mRNA of the CM group were higher than those of the model group; and the WM group(P <0. 01,P <0. 05),ALT and TG of the CM group were; lower than those of the model group (P <0. 01 ). Conclusion HJJB; Compound can significantly increase hepatic IRS1 gene expression and; protein content of fatty liver in rat, and then improve the PI3K/PKB; signal pathways.国家自然科学基金资助项目; 浙江省自然科学基金资助项目; 浙江省中医药科技计划项

Intervention effect of Chinese herb components HJJB Compound on PP1-DNA-PK-USF1 signaling pathway of non-alcoholic fatty liver rats

目的:基于蛋白磷酸酶(PP1)-DNA依赖的蛋白激酶(DNA-PK)-上游刺激因子1(USF1)信号通路,探讨中药组分HJJB方防治非酒精性脂肪; 肝(NAFLD)的作用机制。方法:采用高脂饮食诱导的大鼠NAFLD模型。设模型组、HJJB方组和罗格列酮组,分别灌胃给药6周。HE染色观察肝组织; 病理变化;测定肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化;肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),肝组; 织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较正常组均明显升高(P<0.01)。HJJB方组的上述病理改变明显减轻,肝组织TG、FFA含量较模型组显著降低(P<0.0; 1),肝组织PP1、DNA-PK、USF1; mRNA水平和蛋白含量较模型组显著降低(P<0.01)。结论:HJJB方能显著降低脂肪肝大鼠肝组织PP1; mRNA水平和蛋白含量,进而抑制其下游信号通路,这可能是其防治NAFLD的重要机制。Objective: Toexplore the mechanism of Chinese herb component HJJB; Compound on non-alcoholic fatty liver disease based on PP1-DNA-PK-USF1; signaling pathway. Methods: SD male rats were induced by high-fat diet; for nonalcoholic fatty liver disease model. The rats were randomly; divided into model group, HJJB group and rosiglitazone group, and were; given intragastric administration for six weeks. The observing items; including: liver pathology(HE staining); hepatic triglyceride(TG) and; free fatty acid(FFA) content; mRNA and protein content changes of; hepatic protein phosphatase 1(PP1), DNA-dependent protein kinase(DNA-PK); and upstream stimulating factor 1(USF1). Results: Significant hepatocyte; fatty degeneration and vesicle changes were observed in liver tissue of; model group. The hepatic TG and FFA contents of model group were; significantly higher than those of normal group(P<0.01), and mRNA levels; and protein contents of liver tissue PP1, DNAPK, USF1 in model group; were significantly higher than those in normal group(P<0.01). Hepatic; pathological changes in HJJB Compound group were meliorated, liver; tissue TG and FFA contents of HJJB Compound group were significantly; lower than those of model group(P<0.01), and mRNA levels and protein; contents of liver tissue PP1, DNA-PK, USF1 mRNA of HJJB Compound group; were significantly lower than those of model group(P<0.01). Conclusion:; HJJB Compound can significantly decrease mRNA level and protein content; of PP1 in the liver tissue of fatty liver rats, and then inhibit the; downstream signaling pathway of PP1, which may be an important mechanism; of HJJB Compound for prevention and treatment of non-alcoholic fatty; liver disease.国家自然科学基金项目; 浙江省自然科学基金项目; 浙江省中医药科技计划项

Research of prevention and treatment of herb components HJJB compound on non-alcoholic steatohepatitisin rats induced by high-fat diet

目的:探讨中药组分HJJB方(红景天苷、姜黄素、绞股蓝总苷、白术多糖)对高脂饮食诱导的大鼠非酒精性脂肪性肝炎的防治作用。方法:采用高脂饮食14周; 诱导大鼠非酒精性脂肪性肝炎模型。在造模第9周起,随机分为模型组,HJJB方高、低剂量组,罗格列酮组,灌胃给药6周。观察肝组织病理变化(HE染色); ,肝组织甘油三酯(TG)、游离脂肪酸(FFA)含量的变化,血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、谷氨酰转肽酶(GGT)活性及TG、总胆; 固醇(TC)含量的变化。结果:模型组肝组织出现显著的肝细胞脂肪变性及空泡样变,肝组织TG、FFA含量较正常组显著升高(P<0.01),血清ALT; 、AST、GGT活性及TG、TC含量较正常组亦明显升高(P<0.01)。HJJB方高、低剂量组的上述病理改变显著减轻,肝组织TG、FFA含量及血; 清ALT、AST、 GGT、TG、TC水平显著低于模型组(P<0.01),其中HJJB方高剂量组的肝组织TG、FFA含量和血清ALT、AST、; GGT活性显著低于HJJB方低剂量组和罗格列酮组(P<0.05,P<0.01)。结论:中药组分HJJB方对高脂饮食诱导的大鼠非酒精性脂肪性肝炎具; 有良好的防治作用。Objective: To discuss the prevention and treatment of HJJB (salidroside,; curcumin, gypenoside and atractylodes macrocephala polysaccharide); compound on non-alcoholic steatohepatitisin rats induced by high-fat; diet. Methods: Non-alcoholic steatohepatitis model was induced by; high-fat diet for 14 weeks. From the ninth week, the rats were randomly; divided into model group, HJJB high-dose group, HJJB low-dose group and; rosiglitazone group, and were given gavage for six weeks. The observing; items including: pathological changes of liver tissue (HE staining); the; changesof contents of liver tissue triglyceride (TG) and free fatty acid; (FFA); the activities of serum alanine aminotransferase (ALT), aspartate; aminotransferase (AST), glutamyl transpeptidase (GGT) and contents of; serum total cholesterol (TC) and TG. Results: Significant hepatocellular; steatosis and vacuolar degeneration were observed inthe liver tissue of; the model group. The contents of TG and FFA in liver tissue of model; group were significantly higher than those of normal group (P<0.01), and; the activities of serum ALT, AST, GGT and the contents of TG and TC of; model group were higher than those of normal group too (P<0.01). Hepatic; pathological changes in the HJJB compound high-dose, low-dose groups; were all significantly meliorated. The levels of liver TG, FFA and serum; ALT, AST, GGT, TG and TC of HJJB high-dose group, HJJB low-dose group; were significantly lower than that of model group (P<0.01). In addition,; the contents of liver TG and FFA and serum ALT, AST, GGT of HJJB; compound high-dose group were lower than those of HJJB compound low-dose; and rosiglitazone group (P<0.05, P<0.01). Conclusion: HJJB compound does; well in the prevention and treatment of non-alcoholic steatohepatitis; induced by high-fat diet.国家自然科学基金项目; 浙江省自然科学基金项目; 浙江省中医药科技计划项

玻璃化冷冻和程序化冷冻方法对人类卵母细胞骨架及其发育潜能的影响

目的探讨玻璃化冷冻和程序化冷冻对人卵母细胞纺锤体定位、细胞骨架及其发育潜能的影响。方法将第2日发育为M_II卵母细胞随机分为对照组、程序化冻融组、玻璃化冻融组(解冻0 h、1 h、3 h)。应用液晶偏振光显微镜(Polscope)成像系统观察卵母细胞纺锤体与第一极体(Pb)的夹角、表面积、卵透明带内层光阻值和外层光阻值。采用扫描电子显微镜和透射电子显微镜观察卵母细胞的表面和内部超微结构。统计2种冻融方法对卵母细胞发育潜能的影响。结果对照组、程序化冷冻解冻培养3 h组和玻璃化冷冻解冻后培养0 h、1 h、3 h组中的纺锤体可见率分别为92.4%、56.4%、11.2%、24.8%、61.1%。与程序化冻融组相比,玻璃化冷冻解冻培养3 h后卵母细胞中纺锤体与Pb的夹角更小(37.3°与68°,P=0.023)。对照组、程序化冻融组和玻璃化冻融后培养3 h组中卵母细胞的纺锤体面积、卵透明带内层光阻值和透明带外层光阻值差异无统计学意义(P>0.05)。与程序化冻融组相比,玻璃化冻融后培养3 h组中卵母细胞表面突起丰富,微绒毛形态较为正常,倒伏在细胞表面,卵透明带边界较为清晰,与对照组较为接近。程序化冻融组的正常受精率(65.7%)明显低于对照组(79.2%,P=0.041),而卵裂率和囊胚形成率与对照组和玻璃化冻融组差异无统计学意义(P>0.05)。玻璃化冻融后培养3 h组中正常受精率、卵裂率、囊胚形成率与对照组相比,差异无统计学意义(P>0.05)。结论相比程序化冷冻,玻璃化冷冻对卵母细胞纺锤体和卵透明带的损伤及对卵母细胞的发育潜能的影响都较小,可以作为卵母细胞冷冻的一种有效方法。国家自然科学青年基金(81701419);;国家自然科学基金面上项目(81571418)~

初诊为抑郁症的老年隐球菌性脑膜炎6例报告

目的:探讨老年隐球菌性脑膜炎发病特点和误诊原因。方法:分析6例初诊为抑郁症的老年隐球菌性脑膜炎患者的临床表现、脑脊液特点、治疗和预后表现,以明确发病特点和误诊原因。结果:6例病例初期均表现为头晕、失眠、食纳减少;后期出现颅内感染相对特异症状:认知障碍5例、头痛3例、肢体无力3例、发热2例。脑脊液隐球菌培养阳性明确诊断,抗真菌治疗有效。6例均好转,但是2例患者遗留明显后遗症:1例患者遗留明显的认知障碍和双下肢活动受限,1例双眼失明,2例确诊时间为2、4周。结论:老年患者患病后临床症状常不典型,当一种疾病不能解释所有症状时,需开拓思路,积极完善相关检查,必要时请相关科室协助诊治,尽量减少误诊。福建省厦门市科技局资助项目(项目编号：3502Z20084019

应用调整的膳食平衡指数评价厦门市区成年居民膳食质量

目的利用调整的中国膳食平衡指数(dbI-07)综合评价厦门市城区18~65岁居民膳食质量,为开展营养干预提供依据。方法采用多阶段分层整群抽样方法于2010年10—12月对厦门市区6个社区居委会成年居民进行调查,采用连续3天24 H回顾法对其中的520人进行了膳食调查,采用dbI-07的评分方法评价厦门城区成年居民膳食质量水平。结果被调查的厦门市城区成年居民的膳食质量处于中度失衡状态,摄入不足和摄入过量并存;女性的摄入不足程度高于男性;各年龄段中,40~50岁人群摄入不足程度最高,摄入过量程度最低。结论被调查的厦门市城区成年居民水果、奶类及豆类摄入不足,肉类、食用油及盐类摄入过量;成年居民的主要膳食模式为A模式,且A模式反映了膳食中摄入不足与摄入过量的问题均较少,为最优模式。中央财政转移支付地方项

Study on the variation of the iodine nutrition status among children aged 8 to 10 and pregnant women in Xiamen city after the implement of new standard of iodized salt supply

目的研究新标碘盐供应前后厦门市沿海8-10岁儿童和孕妇的碘营养变化情况,为下一步碘缺乏病防控策略调整提供实证依据。方法在新标碘盐供应前及食用新标碘盐后的第3、6、9个月调查厦门市翔安区8-10岁学生和孕妇的尿碘、尿比重、碘盐覆盖率、碘盐合格率、合格碘盐食用率和居民日人均食盐摄入量。结果自来水水碘含量为4.2μg/l,基线调查、第二、三次评估日人均食盐摄入量均数分别为5.7、6.9、6.9 g;基线调查、第一、第二、三次评估,8-10岁学生碘盐合格率和合格碘盐食用率都分别为97.1、93.1、98.0和98.0,盐碘中位数分别为28.7、24.7、24.0、25.3 Mg/kg,尿碘中位数分别为132.0、177.7、181.7和134.5μg/l,尿比重均值分别为1.0157、1.0196、1.0213和1.0184;孕妇碘盐合格率分别为97.8%、84.6%、84.4%和90.2%,盐碘中位数分别为27.8、25.7、24.3和23.7Mg/kg,尿碘中位数分别为103.8、128.5、138.9和100.2μg/l,尿比重均值分别为1.0123、1.0153、1.0180和1.0143;尿碘与采样季节、气候及尿比重有关,尿碘值与尿比重呈正相关关系。结论新标碘盐实施前后,8-10岁儿童碘营养总体处于适宜水平,但孕妇碘营养均不足,要采取措施加强孕妇碘营养,保障人群健康素质。Objective To investigate the variation of iodine nutrition among children aged 8 to 10 and pregnant women after the implement of new standard of iodized salt supply, and to provide evidence for the further adjustment of control strategy against iodine deficiency disease.Method Survey has been performed to acquire the urinary iodine, specific gravity, iodized salt coverage rate, iodized salt qualification rate, qualified iodized salt consumption rate and average daily salt intake among children aged 8 to10 and pregnant women in Xiamen city.It has been conducted for 3 times repeatedly with a regular interval of 3 months after the implement of new standard.Results The tap water iodine content was 4.2 μg / L.The average daily salt intake of baseline survey, second and third assessment was 5.7, 6.9 and 6.9 g respectively.The iodized salt coverage rates among children aged 8 to10 and pregnant women were all reached 100% in 4 assessments.Among children aged 8 to 10, iodized salt qualification rate and qualified iodized salt consumption rate for 4 assessments were 97.1, 93.1, 98.0 and 98.0 respectively.Median of iodine salt samples was 28.7, 24.7, 24.0 and 25.3 mg / kg, respectively.The median of urinary iodine was 132.0,1 77.7, 181.7 and134.5 μg / L,respectively.The mean of specific gravity was 1.0184, 1.0157, 1.0196 and 1.0213, respectively.Among the pregnant women, the iodized salt qualification rate and qualified iodized salt consumption rate of pregnant women were 97.8, 84.6, 84.4 and 90.2%, respectively.Median of iodine salt samples was 27.8, 25.7, 24.3 and 23.7 mg / kg, respectively.Median of urinary iodine was 103.8, 128.5, 138.9 and 100.2 μg / L,respectively.Mean of specific gravity was 1.0123,1.0153,1.0180 and 1.0143, respectively.Urinary iodine was correlated with season of sampling, climate and specific gravity, and urinary iodine was positively correlated with specific gravity.Conclusion The iodine nutrition status of children aged 8 to 10 was remained in adequate level regardless of the implement of new standard of iodized salt.However, the results suggested that the corresponding indicator among pregnant women was in a relatively low level.Proper countermeasure should be taken to enhance the iodine su-pply among them and to further secure the quality of general population

ST段抬高型急性心肌梗死院前溶栓治疗中国专家共识

急性心肌梗死仍然严重威胁我国人民健康,在我国广大城乡地区,形势更为严峻[1,2]。及时救治急性心肌梗死患者,降低死亡率和保护心脏功能刻不容缓。鉴于我国的实际情况,院前溶栓治疗在大城市以外的城乡地区具有重要意义。为此,中国医师协会胸痛专业委员会及中国医学救援协会心血管急救分会专门组织有关专家制订了本共识,旨在帮助院前医疗急救人员对急性心肌梗死患者选择最佳

Fingerprint analysis on methyl fatty acid and its applications in microbial study

脂肪酸甲酯谱图分析方法(Fatty Acid Methyl Ester,FAME)是鉴于脂肪酸可作为生物标记物而发展起来的分析技术。本文介绍了FAME谱图分析方法及其在微生物学领域的应用,包括在微生物检测、鉴定和微生物多样性研究中的应用。Fatty acid is an important biomarker.Its application in biology research has become increasingly popular.The method of FAME fingerprint analysis is based on fatty acid's utilization as a biomarker.This paper describes the FAME fingerprint analysis and its applications for microbial studies,including identification of unknown microbes and assessment of microbial diversity.国家863计划项目(2006AA10A211);; 福建省发展和改革委员会重点项目[闽农产(2006)10号];; 福建省青年科技人才创新项目(2003.J046

东方肉穗草黄酮类化学成分研究

目的:系统研究东方肉穗草中的黄酮类化学成分。方法:利用大孔吸附树脂、Sephadex LH-20,ODS及正相硅胶柱等色谱手段进行分离,通过多种波谱学数据分析进行黄酮类化合物的结构鉴定。结果:从醋酸乙酯部分分离得到8个黄酮类化合物,经鉴定为异鼠李素(1)、槲皮素(2)、异鼠李素-3-O-β-D-吡喃葡萄糖苷(3)、槲皮素-3-O-β-D-葡萄糖苷(4)、异鼠李素-3-D-(6″-乙酰基)-β-D-吡喃葡萄糖苷(5)、异鼠李素-3-O-(2″-乙酰基)-β-D-吡喃葡萄糖苷(6)、槲皮素-3-O- (6″-乙酰基)-β-D-吡喃葡萄糖苷(7)、槲皮素-3-O-(6″-反式-对香豆酰基)-β-D-吡喃葡萄糖苷(8)。结论:所有化合物均系首次从该属植物中分离鉴定