Effect of Activating Blood and Resolving Stasis Therapy on Injuried Vascular wnt / β-catenin Signal Pathway of Rats with Carotid Atherosclerosis

目的:观察活血化瘀法对颈动脉粥样硬化血管内皮损伤模型大鼠wnt/β-catenin信号通路的影响,探讨其可能的作用机制。方法:32只SD大鼠高脂喂养1个月后,随机取8只作为假手术组(仅切开皮肤),其余大鼠均行颈总动脉球囊损伤术,成膜后再随机分为模型组(n=8只)、活血化瘀中药方中剂量(临床等效量)组(n=8只)和高剂量(临床2倍量)组(n=8只)。从术后第4天开始予以相应药物灌胃治疗(模型组、假手术组灌等量生理盐水),2周后取术侧颈总动脉段,采用实时荧光定量PCR法捡测wnt、Fz D1、AXIN1、GSK-3β、β-catenin、VEGF mRNA基因表达水平。结果:与假手术组比较,模型组wnt、Fz D1、AXIN1、β-catenin、VEGF等mRNA水平降低,差异有统计学意义(P<0.01),经活血化瘀中药方干预后,上述各基因mRNA水平均有不同程度升高,尤其是高剂量组与模型组比较差异有统计学意义(P<0.05~0.01);只有GSK-3βmRNA水平各组无明显差异。结论:活血化瘀法可通过上调wnt/β-catenin信号通路表达,使损伤血管VEGFmRNA基因表达水平上升,提示该法可保护颈动脉粥样硬化血管内皮损伤。Objective: To observe the effect of activating blood and resolving stasis on injuried vascular wnt / β-catenin in carotid atherosclerosis mode rats and its possible mechanism. Methods: After one-month high fat-diet,32 SD rats were randomly divided into sham operation group,model group,middle dose( dose in clinical equivalent) group and high dose of( 2 times the amount of clinical) group. Except for the sham operation group,the other rats underwent carotid artery balloon injury surgery. After 3 days of intraperitoneally administered orally,model group and sham operation group were given equal volume of physiological saline,administered continuously for 14 d. Then the expression of wnt,Fz D1,AXIN1,GSK-3β,β-catenin,VEGF mRNA in injured vascular were analyzed by real-time fluorescence quantitative PCR in all groups. Results: Compared with the sham operation group,the expression of Wnt,Fz D1,AXIN1,β-catenin,and VEGF mRNA levels in model group decreased( P < 0. 01); and after treated by activing blood and resolving stasis therapy,the expression of the above genes increased in different degrees,especially in the high dose group( P < 0. 01 ~ 0. 05); but there was no significant difference among groups on the expression of GSK-3β mRNA. Conclusion: The theapy of activating blood and resloving stasis therapy can increase the expression of VEGF mRNA of balloon injured carotid artery vascular through up-regulating wnt / β-catenin signal pathway,which suggested that it can protect the injured vascular of carotid atherosclerosis.福建省教育厅A类科技项目(编号:JA13165);; 福州市科技计划项目(编号:2012-S-155-1);; 福建中医药大学校管科研项目(编号:X2012018

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Modernizing the Personality of the College Students

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Response Time Analysis for Safety-Critical Hard Real-Time Systems Based on Safety Kernel/Shell Scheme

基于响应时间的可调度分析是设计硬实时系统的基础.防危核(壳)是安全关键硬实时系统的一种新防危措施,现有的普适响应时间分析方法不能适用于这类系统.本文的贡献在于采用检测点时间冗余容错和优先级提升思想,通过分析内/外错误、隐/显错误,提出了一种合适的响应时间分析方法,从而为这类系统提供有效的离线可调度分析工具,同时也为这类系统设计了一种基于检测点的容错方案.本方法稍作修改也可为其它安全关键系统服务.最后通过仿真实验验证了本方法的有效性和优越性.Safety-critical hard real-time systems(in brief,we call them SCHRS below) often operate in harsh environmental conditions that necessitate fault-tolerant computing and safety assurance techniques.Various hardware as well as software safety assurance techniques are employed in these systems among which safety kernel(shell) is a new scheme in application layer and it's correctness can be proofed by formal tool.Appropriate response time analysis schemes(in brief,we call them RTA below) are fundamental to the design of predictable hard realtime systems.However few RTA schemes is suitable to SCHRS based on safety kernel or shell.The main contribution of this paper is twofold.First,we propose an appropriate RTA to improve system fault resilience.Compared with other response time analysis polices,the proposed scheduling analysis takes into account the fact that the recoveries of tasks in safety kernel or shell may be executed at higher priority levels,and there are several kinds of error,such as internal,external,hidden and visible error.This characteristic is very important in SCHRS based on safety kernel or shell.Second,we present a suitable checkpointing fault-tolerant scheme for SCHRS based on safety kernel or shell.The emphasis here is on utilizing this RTA as an off-line design support tool.The effectiveness of the proposed approach is evaluated by simulation.福建省2003年青年科技人才创新基金(No.2003J020);; 福建省2004年自然科学基金(No.A0410004);; 厦门大学院士基金(No.0630-E23011);; 厦门大学新世纪优秀人才支持基金(No.0000-X07116);; 厦门大学985二期信息创新平台项目(No.2004-2007

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近年来,财务报表重述一直受到学术界和监管部门的广泛关注(GA0,2002,2003)。本文利用2007-2009年我国上市公司的数据研究了内部控制对财务重述的治理作用。研究发现:内部控制质量与财务重述显著负相关,内部控制质量越高,越不容易发生财务重述行为,表明内部控制是公司治理财务重述行为的有效措施之一;非政府控制公司的内部控制对财务报表重述具有治理作用,而政府控制公司的内部控制则没有这种治理作用。这可能表明政府控制公司的内部控制"摆设性"更强

Effect of Lunei Xiaoyu Decoction on Vascular Endothelial AMPK/eNOS Signal in Balloon Injury Model of Common Carotid Artery in Rats

目的观察颅内消瘀汤对大鼠颈动脉球囊损伤后血管内皮及AMPK、eNOS表达的影响,探讨其可能的作用机制。方法 32只SD大鼠随机分为假手术组、模型组、颅内消瘀汤低剂量(临床等效量)组和颅内消瘀汤高剂量(临床2倍量)组,除假手术组大鼠仅切开皮肤,其余大鼠均行颈总动脉球囊损伤术以成模。成模第4天灌胃给药,模型组及假手术组给予等体积生理盐水,连续给药14 d后取损伤侧颈总动脉,行HE染色观测血管内皮损伤及内膜增生情况,用免疫组化染色检测eNOS表达水平,并用Western blot检测总AMPK、eNOS蛋白水平。结果颈总动脉球囊损伤模型组大鼠血管内膜增生明显、管壁变厚,颅内消瘀汤低、高剂量组大鼠经治疗后损伤血管增生较模型组轻、管壁较薄;e NOS免疫组化染色:模型组阳性细胞较少,经颅内消瘀汤干预后,低、高剂量2组均有不同程度的表达,与模型组比较差异有统计学意义(P<0.01);Western blot检测结果显示模型组总AMPK、eNOS蛋白表达下降,经颅内消瘀汤干预后,均有不同程度增加,与模型组比较差异有统计学意义(P<0.05或P<0.01)。结论颅内消瘀汤可通过上调AMPK/eNOS信号表达,保护大鼠颈动脉球囊损伤血管内皮的作用。OBJECTIVE To observe the effect of Lunei Xiaoyu Decoction on vascular endothelial AMPK, eNOS expression in ballon injury model of common carotid artery in rats, and to explore its possible mechanism. METHODS Thirty two SD rats were randomly divided into sham operation group, model group, low dose of Lunei Xiaoyu Decoction(dose in clinical equivalent) group and high dose of Lunei Xiaoyu Decoction(2 times the amount of clinical) group, except the sham operation group rats only skin incision, the other rats underwent carotid artery balloon injury surgery. On the 4th day after modeling, the rats were administrated orally for 14 d, model group and sham operation group were given equal volume of physiological saline. Then all the rats were killed, HE staining observation of vascular endothelial injury and intimal hyperplasia, immunohistochemistry staining was used to detect the expression level of e NOS, and Western-blot was used to detect the expression level of AMPK and eNOS. RESULTS The endometrial hyperplasia and thickness were obvious after carotid artery balloon injury in rats; and after Lunei Xiaoyu Decoction treatment, the rats' endometrial hyperplasia and thickness were improved. The expression of AMPKand e NOS was significantly higher in Lunei Xiaoyu Decoction groups compared with the model group(P<0.05 or P<0.01). CONCLUSION Lunei Xiaoyu Decoction can upregulate the expression of AMPK/e NOS signal, in order to protecting vascular endothelial after balloon injured carotid artery in rats.福州市科技计划项目(2012-S-155-1

AMP依赖的蛋白激酶及PI3K/Akt信号途径在颈动脉狭窄形成过程中的变化

目的 观察AMP依赖的蛋白激酶（AMPK）及PI3K/Akt信号途径在颈动脉狭窄形成过程中的变化。方法 取SD大鼠经高脂喂养1个月后行颈总动脉球囊损伤术作为模型组（n=24）,继续喂养14 d后取损伤侧颈总动脉,行HE染色观测血管内皮损伤及内膜增生情况,应用荧光定量PCR法检测AMPK及PI3K/Akt mRNA水平,用Western印迹检测p-AMPK、PI3K、p-Akt蛋白水平,另取8只正常SD大鼠作为假手术组。结果 模型组大鼠血管内膜增生明显、管壁变厚;AMPK/PI3K/Akt mRNA及蛋白水平较假手术组显著下降（P〈0.01）。结论 AMPK/PI3K/Akt在颈动脉狭窄的病理生理进程中起到一定的作用。福州市科技计划项目（No.2012-S-155-1

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Crustins是一类较早发现并广泛分布在甲壳动物中且富含半胱氨酸的阳离子抗菌肽,能够参与抗细菌的免疫应答。从拟穴青蟹（Scylla paramamosain）中克隆获得Crustin基因,命名为SpCrus1a,其cDNA序列全长744 bp,开放阅读框编码113个氨基酸,成熟肽分子量10.03 kDa,等电点8.30。表达分析结果发现其转录本主要存在于鳃、卵巢、上皮组织中,脂多糖刺激后SpCrus1a会上调表达。体外合成SpCrus1a的第29～47位氨基酸进行抗菌活性实验发现,其对革兰氏阳性菌具有较强的抗菌活性,对被测的革兰氏阴性菌抗菌活性较弱或无抗菌活性。合成肽Sp Crus1a29-47能够在较短时间内杀死绝大多数的金黄色葡萄球菌,2 h内杀死全部的金黄色葡萄球菌。扫描电镜发现合成肽Sp Crus1a29-47可造成金黄色葡萄球菌表面结构崎岖不平,高浓度Sp Crus1a29-47造成细菌的大量死亡。上述结果表明,SpCrus1a是抗菌肽Crustins的新变体。国家自然科学基金(U205123,31172438);;厦门南方海洋研究中心科技项目(14PYY050SF03

考虑产品切换的客车混流装配线排序问题

针对主客观因素交互影响下混合装配线的排序问题,建立以最小化工作站堵塞时间与最小化产品切换次数为目标的交互排序模型,并依据客车在实际加工过程中的传统排序方案与模型所得排序方案的结果进行对比分析。模型构建从客观因素出发,优化堵塞时间即“重构”作业框架,以达到对负荷高峰时期进行削峰处理的目的,并进一步在主观因素层面上,考虑线上操作者的作业惯性,降低线上操作者对频繁切换产品的出错率以及保持较高熟练度时操作的方便与流畅性。引入主客观优化评价算法对模型进行计算,决策出最优任务调度方案。对比分析结果表明,模型获得的最优排序改善了装配线的生产堵塞时间,同时兼顾在主观因素主导下的产品切换频率问题,对于节省装配过程中有限的时间资源以及释放有限的空间资源起到很大作用。福建省高校产学合作项目(2017H6020);;福建省科技重大专项(2016HZ0001-9

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内部控制是近年来国内外监管部门、实务界和学术界关注的热点与前沿问题。本文研究我国上市公司最终控制人的产权性质与地区制度环境对公司内部控制质量的影响。利用厦门大学内控课题组(2010)构建的2007-2009年我国上市公司内部控制指数,本文主要发现:首先,相比于中央政府控制的公司,地方政府控制的公司内部控制质量相对较差,而非政府控制的公司内部控制质量与中央政府控制的公司之间则没有明显差异;其次,上市公司所在地区的市场化程度越高或政府对经济的干预程度越低,公司的内部控制质量越高,尤其是对于地方政府控制和非政府控制的上市公司,并且,良好的外部制度环境有助于缩小地方政府控制和非政府控制公司的内部控制与中央政府控制公司之间的差距。本文的研究结果对相关主管部门及监管机构具有一定的政策启示意义