Slow Magnetic Relaxation in Sandwich-Type Tetranuclear Dysprosium Complex with TMe Q[6](TMe Q[6]=α, α, δ, δ-Tetramethylcucurbit[6]uril)

报道了2个基于四甲基取代六元瓜环的三明治型四核稀土簇合物,[ln4(μ3-OH)4(μ2-OH)2(H2O)4(nO3)2(TME Q[6])2]·(nO3)4·26H2O(ln=dy,1;ln=Tb,2)。晶体结构分析显示2个簇合物包含2个四甲基取代瓜环夹心的四核稀土立方烷结构,[ln4(μ3-OH)4]8+。磁性研究显示化合物1显示了慢磁弛豫行为。由于六元瓜环配体可以有效的传递能量给稀土铽离子,化合物2具有较好的发光性能。Two TMe Q[6]-supported sandwich tetranuclear complexes, [Ln4(μ3-OH)4(μ2-OH)2(H2O)4(NO3)2(TMe Q[6])2](NO3)4·26H2O(Ln=Dy, 1; Ln=Tb, 2), have been prepared and characterized.Crystal structural analysis reveals that both complexes contain a cubane-like [Ln4(μ3-OH)4]8+cluster core sandwiched between two TMe Q[6] macrocycles.Magnetic investigations indicate that complex 1 displays slow magnetization relaxation.Complex 2exhibits intense photoluminescence owing to the efficient energy transfer from TMe Q[6] ligand to Tb3+ion.国家自然科学基金(nos.21422106;21371144;21431005)资助项

太平洋白对虾(Penaeus vannamei)β-N-乙酰-D-氨基葡萄糖苷酶在二甲亚砜溶液中的失活动力学(英文)

应用动力学方法研究了太平洋白对虾(Penaeusvannamei)β-N-乙酰-D-氨基葡萄糖苷酶在二甲亚砜溶液中以pNP-β-D-GlcNAc为底物时酶活力的变化规律.表明酶在DMSO浓度低于4.20mol/L,酶的失活过程是可逆的,DMSO并不造成酶绝对量的减少,仅对酶的活力发生可逆的下降.测得DMSO对酶抑制的IC50为1.2mol/L.观测了在不同底物浓度下NAGase在0、0.35、0.70、1.05、1.40、1.75mol/L的DMSO溶液中的失活过程,分别测定了游离酶(E)和酶-底物络合物(ES)的微观失活速度常数k+0和k′+0比较结果(k+0值远远大于k′+0)表明,在DMSO溶液中游离酶比酶-底物络合物更易失活,即底物的存在对于酶被DMSO的失活具有明显的保护作用.随着DMSO浓度的增加,游离酶的逆向微观复活速度常数k-0却不断降低,说明在高浓度DMSO环境中,NAGase可逆恢复的能力逐渐微弱

生物质磺酸钙对荒漠土壤改良作用的研究

在实验室条件下,研究了生物质磺酸钙对荒漠沙土的改良作用。结果表明,沙土中添加2%生物质磺酸钙,土壤中有机质、全氮、速效钾、速效磷含量和土壤持水量均显著增加(P<0.05),土壤pH值由8.4下降...宁夏回族自治区重点研发计划项目(2017BY081)资

计算化学数据与图形在普通化学教学中的运用

鉴于近化学类的非化学专业院系较少开设物理化学和结构化学课程,他们接触到的计算化学知识十分有限,通过对普通化学教学过程中适当引用一些计算化学的模拟研究内容,有利于开拓学生的视野,调动学生学习积极性,更新普通化学教学内容,培养大学生的思维能力,从而提高教学质量。厦门大学校级教改项目(201718

Changes in expression and localization of heat shock protein 70 during curcumin-induced apoptisis of human esophageal cancer cell line EC9706

目的探讨姜黄素对人食管癌EC9706细胞凋亡的诱导作用,热休克蛋白70(HSP70)在肿瘤细胞凋亡过程中在核基质上的变化及其与凋亡调控相关蛋白的关系。方法用细胞计数和流式细胞仪检测姜黄素对人食管癌EC9706细胞的增殖抑制作用,以光学显微镜和透射电镜观察姜黄素诱导人食管癌EC9706细胞凋亡前后的细胞结构变化,琼脂糖凝胶电泳观察人食管癌EC9706细胞凋亡前后的dnA结构变化。双向凝胶电泳和质谱鉴定分析HSP70在核基质中的存在与变化;并以WESTErn blOTTIng进行确证;激光扫描共焦显微镜观察HSP70在EC9706细胞凋亡过程中的定位及其与bAX、bCl-2等基因产物的共定位关系。结果姜黄素能显著抑制人食管癌EC9706细胞增殖并诱导人食管癌EC9706细胞凋亡,双向凝胶电泳、质谱鉴定和结果发现并证实,HSP70在姜黄素处理前后的EC9706细胞核基质蛋白中的存在及其表达下调变化。激光扫描共焦显微镜观察结果显示,HSP70在EC9706细胞凋亡过程中与bAX、bCl-2等基因产物具有共定位关系,且其共定位区域发生了变化。结论姜黄素对人食管癌EC9706细胞具有显著的凋亡诱导作用;HSP70作为一种新发现的核基质蛋白,在姜黄素诱导人食管癌EC9706凋亡过程中的表达与分布发生了显著变化。HSP70与凋亡相关基因的关系对EC9706细胞凋亡具有重要影响。Objective To investigate the effect of curcumin on human esophageal cancer EC9706 cells and explore the role of heat shock protein(HSP70) in cell apoptosis by examining changes in the nuclear matrix and its relationship with apoptosis-related proteins.Methods Cell counting and flow cytometry were performed to probe the inhibitory effect of curcumin on cellular proliferation.Transmission electron microscopy and optical microscopy were used to observe the structural changes in EC9706 cells before and after apoptosis.Agarose gel electrophoresis was conducted to investigate the DNA structure of EC9706 cells before and after apoptosis.Two-dimensional polyacrylamide gel electrophoresis(2-D PAGE) and mass spectrometry(MS) analysis were performed to investigate the presence and changes of HSP70 in the nuclear matrix of EC9706 cells before and after curcumin treatment,which was further corroborated by Western blotting assay.Laser confocal scanning microscopy was used to observe the colocalization of HSP70 with Bax and Bcl-2 during apoptosis.Results The results indicated that curcumin could markedly inhibited EC9706 cell proliferation and finally induced apoptosis.Data from 2-D PAGE,MS,and Western blotting showed that HSP70 was involved in the nuclear matrix proteins and expression of HSP70 was downregulated after curcumin treatment.Laser confocal microscopy showed that HSP70 colocalized with Bax and Bcl-2,and the colocalized regions were altered by the curcumin treatment.Conclusion Our work proves that curcumin could definitely induce EC9706 cells into apoptosis.As a new found nuclear matrix protein,the expression and distribution of HSP70 are altered during the apoptosis of EC9706 cells.The colocalization of HSP70 with apoptosis-related genes evidently affects the apoptosis of EC9706 cells.福建省自然科学基金资助项目(2011J01256); 中央高校基本科研业务费专项资金(2011121061

Apoptosis of Human Esophageal Carcinoma Cell Line EC9706 Induced by Curcumin

目的:应用姜黄素处理人食管癌EC9706细胞,研究姜黄素对人食管癌EC9706细胞凋亡的诱导作用。方法:应用细胞计数、流式细胞仪、琼脂糖凝胶电泳、Hoechst染色、H.E染色和透射电镜检测经姜黄素诱导处理后人食管癌EC9706细胞的凋亡。结果:经姜黄素诱导处理后,人食管癌EC9706细胞生长抑制率达69.9%;细胞周期检测出现亚二倍体(亚G1期)细胞峰值,细胞凋亡率达23%;琼脂糖凝胶电泳显示出细胞凋亡典型的180-200 bp及其倍体的DNA"梯状"条带;Hoechst染色显示细胞核内出现浓染致密的固缩形态或颗粒状荧光;光镜和电镜下可见典型的细胞凋亡特征:细胞体积缩小,染色体凝集,可见有成群或单独存在的凋亡细胞,电镜下可见凋亡小体存在。结论:姜黄素能够有效诱导人食管癌EC9706细胞的凋亡,从而进一步为食管癌等恶性肿瘤疾病的治疗和凋亡机理的研究提供重要基础和科学依据.Objective: To study the apoptosis effects of the human esophageal carcinoma cell line EC9706 cells induced by curcumin(Cur). Methods: The apoptosis effects of EC9706 cells induced by curcumin were detected by cell count, flow cytometry analysis, light microscope and electron microscope. Results: After treated with curcumin, the proliferation of EC9706 cells was inhibited, and the inhibitory rate was 69.9%. The results of flow cytometry analysis showed that curcumin could induce the emergence of the phase of apoptosis, and the rate was 23%. Agarose gel electrophoresis revealed that cell DNA fragment exhibited characteristic "DNA ladder".Cell nucleus concentrated and appeared granular fluorescence by Hoechst33258 staining. Light microscope and electron microscope showed that the morphology of the cells treated with curcumin appeared shrinked, cell nucleus concerntrated, chromatin agglutinated, mitochondria swelled, and apoptosis body formed. Conclusion: This study suggested that curcumin could induce apoptosis of the human esophageal carcinoma cell line EC9706 effectively, and provided important foundation and research proofs to study more about the therapy of esophageal carcinoma, the malignant tumor and apoptosis mechanisms.国家自然科学基金项目(30470877

Expression and Localization of hnRNP A2/B1 during Differentiation of Human Osteosarcoma MG-63 Cells Induced by HMBA

背景与目的:核基质蛋白的差异表达与细胞癌变和增殖分化调控关系密切。本研究观察了hnRNP A2/B1在诱导分化处理前后人成骨肉瘤MG-63细胞核基质中的存在和分布,及其与Actin、Prohibitin的共定位关系。方法:选择性抽提经环六亚甲基双乙酰胺(hexamethylene bisacetamide,HMBA)诱导处理前后的MG-63细胞核基质,并运用双向电泳、质谱分析、蛋白质印记杂交、免疫荧光、激光共聚焦等技术检测hnRNP A2/B1在核基质中的表达与定位变化,及其与相关蛋白的共定位关系。结果:双向电泳及蛋白质印迹杂交结果证实了hnRNP A2/B1存在于MG-63细胞核基质蛋白组分中,并在HMBA处理后细胞核基质中表达下调;免疫荧光显微镜观察显示hnRNP A2/B1定位在核基质上,经HMBA处理后hnRNP A2/B1表达减弱。激光共聚焦显微镜观察结果显示,hnRNP A2/B1与细胞核基质蛋白组分Actin、细胞增殖相关调控因子Prohibitin具有共定位关系,但在诱导处理后细胞内的共定位关系减弱。结论:hnRNP A2/B1在MG-63细胞诱导分化过程中的表达分布,及其与Actin、Prohibitin的共定位关系的改变对MG-63细胞分化具有重要影响,值得进一步探索和研究。BACKGROUND & OBJECTIVE: The differentially expressed nuclear matrix proteins have great effects on canceration and regulation of cell differentiation. This study was to explore the existence and distribution of ribonucleoprotein hnRNP A2/B1 in nuclear matrix and its co-localization with Actin and Prohibitin in human osteosarcoma MG-63 cells before and after hexamethylene bisacetamide (HMBA) treatment. METHODS: The nuclear matrix of MG-63 cells before and after treatment of HMBA were selectively extracted. The expression and localization of hnRNP A2/B1 in nuclear matrix were detected by 2-D PAGE, MALDI-TOF-MS, Western blot, and immunofluorescent staining. The co-localization of hnRNP A2/B1 with Actin and Prohibitin was observed under laser scanning confocal microscope (LSCM). RESULTS: hnRNP A2/B1 was detected in the component of nuclear matrix proteins of MG-63 cells by Western blot and immunogold staining and its expression was decreased after treatment of HMBA. hnRNP A2/B1 was located in the nuclear matrix, and its expression was weakened after HMBA treatment. hnRNP A2/B1 was co-localized with Actin or Prohibitin in MG-63 cells, while the co-localization relationship was weakened during differentiation of MG-63 cells. CONCLUSIONS: hnRNP A2/B1 is a kind of nuclear matrix protein, and localizes in the nuclear matrix. The distribution and expression of hnRNP A2/B1 and its co-localization with Actin and Prohibitin play important roles during the differentiation of MG-63 cells.国家自然科学基金项目(No.30470877)~

Establishment of a method to quantitatively detect FLT3 internal tandem duplication in acute myeloid leukemia with denaturing high-performance liquid chromatography

目的:建立一种应用变性高效液相色谱技术(dHPlC)相对定量检测急性髓细胞白血病(AMl)患者fMS样酪氨酸激酶3(flT3)基因的内部串联重复(ITd)突变的方法。方法:根据flT3-ITd突变基因多位于14外显子而设计引物,用聚合酶链反应(PCr)方法特异性扩增121例AMl患者flT3-ITd突变基因,再用dH-PlC技术相对定量检测flT3-ITd等位基因突变的情况;与毛细管电泳法(CE)检测突变的结果对比进行该方法的有效性检验;最后与121例样品PCr扩增产物的测序结果进行对比。结果:经dHPlC分析后均能得到特征性的洗脱峰。121例样本中检测到flT3-ITd突变阳性的样本13例,总阳性率为10.7%,阳性突变等位基因的比例不一,分布范围中位数为34.5%(11.4%-80.2%),为21-87 bP单个插入片段。阳性率和突变比例与CE方法检测结果相比较均无显著差异(P>0.05),并与121例样本flT3-ITd扩增PCr产物基因测序结果一致。结论:成功建立了一种应用dHPlC相对定量检测AMl患者flT3-ITd基因突变的方法。AIM: To establish a relatively-quantitative method to detect the internal tandem duplication(ITD) mutation of Fms-like tyrosine kinase 3(FLT3)gene in acute myeloid leukemia(AML) patients using denaturing high-performance liquid chromatography(DHPLC).METHODS: According to the fact that much more FLT3-ITD mutations are located in exon 14,we designed the primers,and use the method of polymerase chain reaction(PCR) to specifically amplify FLT3-ITD mutation gene in 121 cases of AML,and relatively quantified the situation of mutant allelic gene of FLT3-ITD by the method of DHPLC.The effectiveness of DHPLC was verified by the method of capillary electrophoresis(CE).The sequenced results from PCR amplified products of 121 samples were compared.RESULTS: A characteristic of elution peak was detected by DHPLC with 10.7% overall positive rate(13/121) and varied in the proportion of mutant alleles,with a single duplicated insert fragment from 21 bp to 87 bp.The median range of mutant alleles was 34.5%(11.4%-80.2%).No significant difference of the positive rates and mutation proportions between the results with DHPLC and the results with CE method was observed.The results of FLT3-ITD mutant gene of 121 samples were consistent with the results using sequencing method.CONCLUSION: A relatively-quantitative method to analyze AML patients with FLT3-ITD mutation by DHPLC is successfully established

Assessing ecological risks of heavy metals to marine organisms in the Jiulongjiang Estuary by species sensitivity distribution

采用物种敏感性分布法(SSd)构建常见重金属元素对海洋生物的SSd曲线,结合九龙江口水体5、8、11月份21个站位重金属调查数据,计算了九龙江口7种重金属(AS,Cd,Cr,Cu,Hg,Pb,zn)不同暴露浓度对海洋生物的潜在影响比例(PAf),并分析了在相应站位重金属复合污染生态风险(MSPAf)。结果表明,7种重金属中AS的生态风险最大(即PAf值最高);时间尺度上,5月份总的MSPAf较其他月份稍高;空间尺度上,西溪至海门岛(1~7号站位)污染较严重,其中又以位于西溪和玉枕洲的2号和5号站位的MSPAf为最。In the present work,a species sensitivity distribution( SSD) method was used to assess the ecological risk of common heavy metals to marine organisms.The ecological risk was characterized by potentially affected fraction( PAF) of species in relation to concentration of the toxic materials.According to the investigation data obtained at 21 stations in the Jiulongjiang Estuary in May,August and November,the PAFs of seven heavy metals( As,Cd,Cr,Cu,Hg, Pb,and Zn) to marine organisms were calculated.The results showed that As had the highest PAF among the seven heavy metals at each station of the Jiulongjiang Estuary.Spatially,the multi-substance PAF( msPAF) in May was higher than in other months.Temporally,the msPAF of the Stations 1-7( from the Xixi Stream to the Haimen Isle of the Jiulongjiang Estuary) suffered from heavier pollution.In particular,the pollution status of the Station 2 and Station 5 was the most serious among all the investigated stations.海洋公益性行业科研经费专项(201105015); 国家海洋局青年海洋科学基金项目(2011143); 国家自然科学基金项目(31101902); 福建省自然科学基金项目(2012J05074); 国家海洋局第三海洋研究所基本科研业务费专项(海三科2011006)资

中国海洋生物研究70年

随着中国"海洋强国"战略的提出,加快建设海洋类学科的发展成为历史必然,海洋生物是海洋不可分割的一部分,海洋环境和生物相互依存,相互作用,海洋生物研究重要性日益凸显。为纪念中国科学家在海洋生物领域的突出贡献,本文回顾了建国以来中国海洋生物相关的重要研究进展,梳理了中国科学家在海洋生物领域的突出贡献,系统总结并讨论了未来研究方向,抛砖引玉,希望籍此助推中国海洋生物研究的新高潮。国家自然科学基金项目(41876134,41876171)中国大洋矿产资源研究开发协会专项项目(DY135-E2-5-03)教育部长江学者特聘教授项目(T2014253