Effects of electropuncture of acupoints on the foot Yangming stomach meridian on metabolic profiling of gastric mucosa in gastric ulcer rats

目的:采用核磁共振氢谱技术(1H; NMR)研究电针胃经穴对应激性胃溃疡大鼠胃黏膜组织代谢物谱的表达。方法:40只SD大鼠随机分为正常组、模型组、胃经组和胆经组。利用束缚-冷应激法; 制备应激性胃溃疡模型大鼠,光镜下观察大鼠胃黏膜组织病理形态学的变化,采用1H NMR技术获取胃黏膜组织1H; NMR谱,并利用模式识别方法分析各组间的代谢物谱的差异。结果:与正常组比较,模型组大鼠胃黏膜损伤和胃黏膜组织代谢轮廓差异明显,胃黏膜组织乙酸、肌; 醇、磷酸胆碱、谷氨酸、甘氨酸、乙醇胺、赖氨酸、3-羟基丁酸、缬氨酸、异亮氨酸、牛磺酸、亮氨酸和甘露醇含量上升(P<0.05),乳酸、甲硫氨酸和天; 冬氨酸含量下降(P<0.05);与模型组比较,胃经组和胆经组大鼠胃黏膜得到明显修复,胃经组大鼠胃黏膜组织代谢轮廓更接近正常大鼠,胃黏膜组织甲硫氨; 酸、天冬氨酸含量上升(P<0.05),乙酸、肌醇、磷酸胆碱、谷氨酸、甘氨酸、赖氨酸、异亮氨酸、牛磺酸、乳酸、亮氨酸和甘露醇含量下降(P<0.05; );胆经组大鼠胃黏膜组织3-羟基丁酸、甲硫氨酸含量上升(P<0.05),肌醇、磷酸胆碱、谷氨酸、乙醇胺、异亮氨酸、乳酸、亮氨酸和甘露醇含量下降(; P<0.05)。结论:电针胃经穴可调节胃溃疡大鼠胃黏膜组织特异代谢物的表达,促进胃黏膜损伤的修复。Objective: To research the effects of electropuncture of acupoints on; the foot Yangming stomach meridian on metabolic profiling of gastric; mucosa in rats of gastric ulcer induced by stressusing the 1H nuclear; magnetic resonance (1H NMR). Methods: Forty SD rats were randomly; divided into the control group, the model group, the stomach meridian; group and the gallbladder meridian group. The rat model of gastric ulcer; was induced by the restrained plus cold stress. The pathological changes; in the rat gastric mucosa tissue were observed by light microscope. The; 1H NMR spectroscopy was determined with the 1H NMR technique. And,; meanwhile the differences in the metabolic profiling were identified; with the pattern recognition method. Results: Compared to normal group,; the gastric mucosa damage and gastric mucosa metabolic profiling are; obvious, the concentration of acetic acid, choline, inositol phosphate,; glutamic acid, glycine, ethanolamine, lysine, 3-hydroxy butyric acid,; valine, isoleucine, taurine, leucine and mannitol increase and the; concentration of lactic acid, methionine and aspartic acid decrease in; model group rats (P<0.05). Compared to model group, the gastric mucosa; damage have been recovered in stomach meridian group and gallbladder; meridian group, the metabolic profiling of gastric mocusa tissue in; stomach meridian group are similar to the normal group. The; concentration of methionine, aspartic acid increase and acetic acid,; choline, inositol phosphate, glutamic acid, glycine, lysine, lactic; acid, leucine, isoleucine, taurine, and mannitol decrease in stomach; meridan group (P<0.05). The concentration of 3-hydroxy butyric acid,; methionine increase and choline, inositol phosphate, lactic acid,; leucine, isoleucine, glutamic acid, ethanol amine and mannitol decrease; in gallbladder meridian group (P<0.05). Conclusion: The electropuncture; of acupoints on the foot Yangming stomach meridian could regulate the; metabolic profiling of gastric mucosa, resulting in improving the; gastric mucosa repair of gastric ulcer rats.国家自然科学基金项目; 国家重点基础研究发展计划(973计划)项目; 广东省自然科学基金项目; 深圳市科技计划项

Ge/GeO/多层石墨纳米复合物锂离子电池负极材料性能研究

由于其高容量、快速锂离子扩散速率和高电导性的优点,锗被认为是一种非常有前景的锂离子电池负极材料.本研究利用GeO2和石墨作为前驱体,通过水热法制备Ge/GeO/多层石墨复合物并将其应用于锂离子电池负极材料进行电化学性能研究.实验结果表明,Ge/GeO2纳米粒子的粒径约为40 nm.该复合物电极的第一次充放电容量分别是2045和1146 mA h g.1,库仑效率为56.0%.50圈充放电循环后,当电压范围为0.01.50 V时,容量保持在1008 mAhg.倍率实验表明,该电极在1C(1C=1000mAg)和2 C倍率大电流下,虽然容量略有衰减,但仍保持790和710 mAhg的高容量.教育部新世纪优秀人才计划(编号:NCET-13-0879)；福建省闽江学者特聘教授计划；泉州市桐江学者特聘教授计划；福建省自然科学基金(编号:2016J01069)资助项目

GaN LED/metals/Si structure fabricated by bonding and laser-lift off

采用金属键合技术结合激光剥离技术将gAn基lEd从蓝宝石衬底成功转移到SI衬底上。利用X射线光电子谱(XPS)研究不同阻挡层对Au向gAn扩散所起的阻挡作用,确定键合所需的金属过渡层。利用多层金属过渡层,在真空、温度400℃和加压300 n下实现gAn基lEd和SI的键合,通过激光剥离技术将蓝宝石衬底从键合结构上剥离下来,形成gAn基lEd/金属层/SI结构。用金相显微镜及原子力显微镜(AfM)观察结构的表面形貌,测得表面粗糙度(rMS)为12.1 nM。X射线衍射(Xrd)和rAMAn测试结果表明,衬底转移后,gAn基lEd的结构及其晶体质量没有发生明显变化,而且gAn与蓝宝石衬底间的压应力得到了释放,使得SI衬底上gAn基lEd的电致发光(El)波长发生红移现象。The effect of different barrier layers on gold diffusing into GaN is analyzed by X-ray photoelectron spectroscopy(XPS).By using wafer bonding and laser lift-off(LLO),which uses a KrF excimer laser(248 nm) to separate GaN LED from sapphire substrate,an light emitting diode(LED) GaN epi layer is successfully transferred onto a Si substrate at the temperature of 400 ℃.The surface of samples after laser lift-off(LLO) is observed with microscope and atomic force microscope(AFM).The root-mean-square roughness of the transferred GaN LED surface is about 12.1 nm.The X-ray diffraction(XRD) and Raman test results show that the quality of LED on Si substrate has not obviously change,but the electroluminescence peak wavelength of GaN-based LED on Si substrate showes a red shift compared to that on sapphire substrate.国家自然科学基金重点基金资助项目(60837001);福建省自然科学基金资助项目(2008J0221);福建省教育厅科技项目(JB08215

Establishment and Characterization of a Targeting Tumor Vesseles System for Inducing Thrombosis

目的建立一种新型的靶向高效诱发肿瘤血管血栓栓塞体系磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子(Mf-A6-SA:b-TTf)。方法化学交联技术制备神经纤维网蛋白1抗体A6-链霉亲和素、磁流体-神经纤维网蛋白1抗体A6-链霉亲和素和生物素-短截型组织因子交联物,凝血因子X活化实验鉴定复合体系活化凝血因子X的活力,荧光显微镜技术和普鲁士蓝染色法同时观察施加外界磁场后复合体系的靶向作用,凝血实验直接观察复合体系引入链霉亲和素:生物素的生物放大效应,体内生物分布实验观察复合体系的安全性。结果成功制备磁流体-神经纤维网蛋白1抗体A6-链霉亲和素及生物素-短截型组织因子,磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子体系保留有高效激活凝血因子X的活性,与靶点的结合具有靶向性及高效富集性,体内实验证实能安全有效诱发肿瘤血管栓塞。结论成功制备的具有靶向诱发肿瘤血管血栓栓塞体系磁流体-神经纤维网蛋白1抗体A6-链霉亲和素:生物素-短截型组织因子为进一步探索肿瘤血管的靶向治疗奠定基础。OBJECTIVE To establish a novel targeting tumor vessels system MF-A6-SA:B-tTF for efficiently inducing thrombosis.METHODS Chemical cross-linking technique was used to prepare a cross-linking agent of the A6-Streptavidin( A6-SA),MFA6-SA and Biotein-tTF(B-tTF).FX coagulation assay was used to test MF-A6-SA:B-tTF system's FX activity.Fluorescence microscopy and prussian blue staining were used to simultaneously observe the targeting activity of MF-A6-SA:B-tTF with an external magnetic field.Hemagglutination was directly used to study the system' s biological amplification by SA /B.Biodistribution experiment was used to observe the toxicity of MF-A6-SA:B-tTF.RESULTS MF-A6-SA and B-tTF were successfully prepared.MF-A6-SA:B-tTF system could activate FX,inducing the blood coagulating cascade powerfully.MF-A6-SA:B-tTF could be accumulated to the desired target area with targeting and induce thrombosis in tumor blood vessels in vitro and in vivo.CONCLUSION The double targeting tumor vessels system MF-A6-SA:B-tTF maybe provide a basis for developing the tumor blood vessels targeting therapy.国家自然科学基金资助项目(30973485;81172970); 国家级大学生创新性实验项目(201210384134

The influence of mindfulness on sleep quality and its intervention: the mediating role of self-control

睡眠是人类最重要的生理活动之一，对人的身心健康起着重要的作用，有研究发现具有更多积极情绪、较强自控力的个体有更少的睡眠问题。在当前的时代背景下，有越来越多的人对宗教信仰、正念感兴趣。正念来源于佛教的禅修，且有研究发现，正念练习能提高个体的积极心理情绪和自控能力。然而，目前国内外少有研究佛教徒和无信仰人群的正念和自控力、睡眠的相关关系，对其的关系和机制尚不明确。研究其的关系对未来睡眠质量的调节和改善具有重要意义。本研究运用中介模型和一个正念干预实验，探索了（1）佛教徒与无信仰人群中正念水平对睡眠的影响：自控力的中介作用；（2）正念干预通过提高正念水平和 自控力改善睡眠质量。希望通过新的视角，为正念干预改善睡眠提供新的理论支持。 在研究一中，采取方便抽样，选取 765 份成年人进行问卷调查，采用一般人 口学资料、正念五因素量表、匹兹堡睡眠质量指数量表以及自我控制问卷进行施测，通过 SPSS26.0 统计软件对数据进行分析，建立中介模型。研究二在线上招募有佛教信仰但无禅修经验和正念经验的被试，选取匹兹堡睡眠质量量表得分>5分的成年人被试 22 名进行为期 6 周每周 1 次的干预实验，以同样的标准招募 75 名被试，通过性别、受教育程度和 PSQI 量表得分进行匹配，选取 22 人进入对照组进行空白对照。测量两组被试在正念干预前后的正念水平、自控力水平和睡眠质量水平。所有数据经双人审核后应用 SPSS26.0 进行统计学分析，统计方法主要包括方差分析、配对样本 t 检验、非参数检验、相关分析及回归分析。 结果：（1）在两组被试中除独居因素单独影响无信仰组外，其他对睡眠质量有显著影响的因素在两组结果中均相同；（2）正念水平高组睡眠问题的检出率低于正念水平低组；在加入自控力做为中介变量后，直接效应消失，自控力在正念水平与睡眠质量中起完全中介作用。（3）正念干预六周后，干预组在正念水平及其观察、描述、不反应维度，自控力水平及其冲动控制、健康习惯维度的得分均有显著提高；睡眠状况及其睡眠质量、入睡时间、睡眠时间、睡眠问题和日间功能障碍维度的得分均有显著降低。正念水平前后测变化量与自控力水平前 后测变化量、睡眠质量水平前后测变化量分别呈现显著正相关与负相关。 结论：（1）研究一发现了在两类人群中正念水平对于睡眠质量均存在正相关，且均通过自控力的中介效应来实现。（2）在控制宗教信仰和禅修练习、正念练习变量的情况下，验证了正念干预对正念水平、自控力和睡眠质量的积极影响，基本验证了研究一结果二中正念水平、自控力和睡眠质量的相互关系。为正念干预改善自控力和睡眠提供新的理论支持

Development and reliability and validation of the Trait Mindfulness Scale

目的 编制适用于我国大众的本土化特质正念量表并检验其信效度。方法 通过文献分析、深度访谈和专家评定确定初始量表的操作性定义、维度和条目。使用网络问卷对498人进行预测试。形成正式量表后，对472人进行正式施测并检验信效度。使用正念注意觉知量表和五因素正念量表作为效标工具，并考察特质正念量表与心理健康的相关性。结果 特质正念量表包含29个条目，包含觉察、专注、接纳、不反应四个维度。Cronbach&prime;s &alpha;系数为0.950,各维度为0.879～0.924,重测信度良好。验证性因子分析显示四因子模型拟合良好，多数因子载荷在0.6～0.9之间。量表得分与两个正念量表以及两个心理健康量表显著相关，具有良好的效标关联效度。有正念经验者在总分和各维度上显著高于无正念经验者。结论 特质正念量表各项测量学指标良好，可作为我国大众特质正念水平的评估工具。</p

Mediating effect of self-control between mindfulness experience and sleep disorder

目的 探讨正念经验与睡眠障碍的作用机制。方法 采用匹兹堡睡眠量表(PSQI)、自我控制问卷(SCS)对860名被试进行线上测量。结果 无正念经验组中睡眠障碍的检出率明显高于有正念经验组。自控力在正念经验与睡眠障碍中起部分中介作用。结论 正念经验与较高睡眠质量相关，正念练习可部分通过提高自控力水平发挥作用。</p

XPS Study of the Interfaces of Low-temperature Si/Si Wafer Bonding

利用金属过渡层的方法实现了SI/SI低温键合.拉力测试表明,温度越高,键合强度越大.采用X射线光电子能谱对SI/SI键合界面进行了研究,结果表明,退火样品的界面主要为Au-SI共晶合金;SI-Au含量比随着退火温度的升高和刻蚀深度的增加而增大.Low-temperature wafer to wafer bonding has been achieved by using intermediate metals.Tensile test shows that the higher the bonding temperature is.The greater the bonding strengthis.The Si/Si interfaces are analyzed by using X-ray photoelectron spectroscopy(XPS).The results shows that the interface of the samples is made up of Au-Si eutectic alloy and the ratio of Si-Au increases with the annealing temperature increasing.国家自然科学基金重点基金资助项目(60837001);福建省自然科学基金资助项目(2008J0221);福建省教育厅科技项目(JB08215

Polygonatum sibiricum polysaccharides attenuate cadmium-induced bone damage, abnormal metabolism of bone marrow mesenchymal stem cells and disorder of gut microbiota(黄精多糖改善镉诱导的骨损伤、骨髓间充质干细胞代谢异常和肠道菌群紊乱)

Exposure to cadmium (Cd) can damage the bone microstructure and cause osteoporosis, but there is currently a lack of effective intervention strategies. In this study, a rat model of Cd exposure (drinking 50 mg/L CdCl2 aqueous solution for 8 weeks) and an in vitro model of osteogenic differentiation of bone marrow mesenchymal stem cells (BMMSCs) were established to explore the bone-protective effect of Polygonatum sibiricum polysaccharides (PRPs). The results showed that PRPs intervention could significantly improve the bone microstructure of Cd-exposed rats and repair the damage to the ileal villus structure. Immunohistochemical analysis revealed that PRPs intervention up-regulated the expressions of alkaline phosphatase, osteocalcin and bone morphogenetic protein-4 of osteogenic differentiation markers (P＜0.05). In vitro experiments confirmed that PRPs alleviated the Cd-induced inhibition of BMMSCs viability (P＜0.05) and promoted osteogenic differentiation (with increased ALP activity and decreased number of mineralized nodules, P＜0.05). Metabolomic analysis indicated that PRPs mainly exerted their protective effects by regulating central carbon metabolism, amino acid biosynthesis, and protein digestion and absorption pathways. Gut microbiota analysis showed that PRPs significantly increased the abundance of Lactobacillus by regulating the structure of gut microbiota. In conclusion, PRPs can attenuate Cd-induced bone damage, abnormal metabolism of BMMSCs, and gut microbiota disorders, providing a new strategy for precise nutritional intervention.(镉（cadmium, Cd）暴露可导致骨微结构损伤及骨质疏松症，但目前缺乏有效干预策略。本研究通过构建Cd暴露大鼠模型（饮用50 mg/L CdCl2水溶液8周）及骨髓间充质干细胞（bone marrow mesenchymal stem cells, BMMSCs）成骨分化体外模型，探讨黄精多糖（Polygonatum sibiricum polysaccharides, PRPs）的骨保护作用。结果显示：PRPs干预可显著改善Cd暴露大鼠骨微结构，并修复回肠绒毛结构损伤。免疫组织化学分析显示，PRPs干预可上调成骨分化标志物碱性磷酸酶、骨钙素及骨形态发生蛋白-4表达（P＜0.05）。体外实验证实，PRPs缓解Cd诱导的BMMSCs活力抑制（P＜0.05），同时促进成骨分化（ALP活性提高，矿化结节数减少，P＜0.05）。代谢组学分析表明，PRPs主要通过调控中枢碳代谢、氨基酸生物合成及蛋白质消化吸收通路来发挥保护作用。肠道菌群分析表明，PRPs通过调节肠道菌群结构，显著提升了乳杆菌属菌群的丰度。综上所述，PRPs可改善Cd诱导的骨损伤、BMMSCs代谢异常和肠道菌群紊乱，为精准营养干预提供了新策略。