Inducement of the CYP1A1 mRNA Expression and EROD Activity of Black porgy(Sparus macrocephalus) Exposed to Benzo(a)pyene

在对水生生态系统污染监测中,鱼类CYP1A的诱导已被作为暴露于PAHs、PCBs、二噁英以及相关化合物的早期预警信号及高度敏感的生物反应。本研究利用生物学方法探讨PAHs的典型代表物BaP暴露对养殖黑鲷的CYP1A1mRNA的诱导和对肝脏EROD活性的影响，以期在分子水平筛选出合适的生物标志物，检测水环境中有机污染。取得以下结果：1.从养殖黑鲷（Sparusmacrocephalus）的肝脏中克隆得到CYP1A1cDNA（Genbank登录号DQ898145），经过序列分析比对，和鲷科其它鱼类的CYP1AcDNA同源性大于90％，含有P450cDNA和CYP1AcDNA的特征序列，是CYP1A...CYP1A gene is highly inducible by polyaromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCB’s),dioxins and related compounds.Induction of CYP1A1 in fish has been evaluated as a sensitive method in monitoring contamination in aquatic systems. The research detect the effect of BaP, typical representative of PAHs, on the caged black porgy by measure of liver organs CYP1A1 mRNA expression and t...学位：工学硕士院系专业：海洋与环境学院环境科学与工程系_环境科学学号：20033400

从自贸区迈向自由贸易港:国际比较与中国的选择——兼析厦门自由贸易港建设

自贸区发展应在税收制度、政府职能转变、金融开放、贸易监管和投融资管理等方面与国际高度开放贸易准则对接,形成改革引领作用,带动区域经济协调发展。建立中国特色的自贸港应在以下几方面所有创新:扩大税收优惠政策,优化政府管理体制,创造更有吸引力的投资环境,保证人员、金融、物流和公共服务这四个方面自由流动,做好配套的金融制度改革。国家自然科学基金项目“动态优化视角下的中国外汇储备全面风险管理研究”(71473280);;中央高校基本科研业务专项之山海基金:两岸金融合作创新与具体方案设计(20720140008);;福建省发改委资助项目:深化闽台经济合作新模式研

Cloning and sequence analysis of hepcidin-like cDNA Hepc2 from liver of Lateolabrax japonicus

Hepcidin(Hepc)是一类性质独特的抗菌肽,具有广谱的抗革兰氏阳性、阴性细菌和真菌等作用。利用RT-PCR和RACE等技术,从经过多种细菌攻毒的花鲈(Lateolabrax japonicus)肝组织中克隆出Hepc全长cDNA,命名为Hepc2,Genbank登录号为AY604195。Hepc2有581个碱基,其中阅读框有258个碱基,编码86个氨基酸。预测氨基酸序列和白鲈及其他鱼种在相同保守的区域有8个半胱氨酸,相对分子量为9418.55dal。在3’非编码区有225bp,包含终止密码子下游189nt处的多腺苷酸化AATAAA信号和212nt处的polyA信号。预测蛋白的信号肽断裂位点在第24和第25个密码子之间。通过与白鲈(Morone chrysops)、人和其他鱼种来源的Hepc cDNA和蛋白质同源性分析表明,从花鲈肝中分离的Hepc2 cDNA属于Hepc基因家族的新成员。Hepcidin is a unique antimicrobial peptide which exerts broad-spectrum antimicrobial action against Gram-negative and Gram-positive bacteria,as well as fungi.A Hepc-like cDNA was amplified from the liver of Lateolabrax japonicus challenged with a mixed bacteria solution.Using RT-PCR and RACE with a specific primer pair,a full length cDNA sequence Hepc2 of the Hepc-like antimicrobial peptide(GenBank accession number:AY604195) was obtained.Hepc2 cDNA is composed of 581 bases,which contains an ORF of 258 bases,encoding 86 amino acids.The deduced amino acid sequence is conserved between white bass and other fish species,which share eight cysteines at the identical conserved position.The relative molecular weight of the protein is 9418.55dal.The 3′non-coding region is composed of 225bp,with a polyadenylation signal AATAAA sequence appearing at position 189 nt,and poly(A) tail at 212 nt,downstream codon TAA.The signal peptide cleavage site of its deduced protein is presumed between codon 24 and 25.High homologies with Hepc cDNAs and proteins of white bass(Morone chrysops),human and other fish are shown.It indicates that Hepc2 cDNA from Lateolabrax japonicus liver is a new member of the Hepc gene family.福建省重大科技项目(2003I005);; 厦门市高新技术项目(3502Z20021052)资

漳浦县石寨窑发掘简报

漳浦县石寨窑址是闽南一处专门仿烧龙泉青瓷的窑场,从元代开始,一直延续到明初。其产品种类较少,釉色比较丰富。窑业技术颇具特色,出现了介于分室龙窑和横室阶级窑之间的窑炉结构,对研究闽南地区横室阶级窑的起源与发展具有重要意义

Genomic organization and tissue-specific expression analysis of hepcidin-like genes from black porgy (Acanthopagrus schlegelii B.)

【英文摘要】Hepcidin is an antimicrobial peptide and putative iron regulatory hormone previously described in mice and humans. Dozens of fish hepcidins have been isolated and characterized so far. Here we present seven hepcidin-like cDNA sequences named AS-hepc1–7, amplified from the normal commercially cultured fish (black porgy) by RACE–PCR. Sequence analysis reveals that these seven potential hepcidin peptides have highly conserved sequences with other known hepcidins, but they are different from each other in constitution and characteristics of predicted mature amino acids. Based on the study, it is deduced that AS-hepc1–7 represent different variants of a family of hepcidin genes in black porgy. To understand the organization of these hepcidin-like genes, we sequenced AS-hepc2 DNA, AS-hepc3 DNA, AS-hepc4 DNA, AS-hepc7 DNA and AS-hepc2 upstream region; and all of the four genomic DNAs consisted of two introns and three exons, the same organization as other reported hepcidins. The tissue-specific gene expression of hepcidins in normal black porgy was evaluated using RT–PCR and dot blot approaches. RT–PCR showed that transcripts of hepcidin-like mRNAs were present in each tested tissue of normal juvenile black porgy, including liver, spleen, kidney, heart, brain, stomach, intestine, gill, skin and blood, but abundant hepcidin-like mRNA transcripts were only detected in the liver, kidney, spleen, intestine and stomach by dot blot assay. In addition, using dot blot and Northern blot approach, a significant increase of hepcidin mRNA transcription was observed in the liver within 48 h after immersion in a suspension of live bacteria, which suggested that the expression pattern of hepcidin-like genes in black porgy might be different in the liver from the other tissues as previously reported in several hepcidin studies

Distribution, degradation and dynamics of dissolved organic carbon and its major compound classes in the Pearl River estuary, China

We investigated the distribution, degradation and dynamics of organic carbon and its major compound classes, carbohydrates and amino acids, based upon a cruise in the Pearl River estuary in April 2007. Dissolved oxygen (DO), nutrients, particulate organic carbon (POC), chlorophyll a (Chl a), dissolved organic carbon (DOC), total dissolved carbohydrates (TCHO, including monosaccharides, MCHO, and polysaccharides, PCHO) as well as total dissolved amino acids (TAA, both dissolved free, DFAA, and combined components. DCAA) were measured along a salinity gradient. Community respiration and biodegradable DOC were also determined via both short term (within 3 days) and long term (lasting 30 days) incubation. DOC, MCHO, TCHO, DFAA and TAA concentrations were high in the upper reach of the Pearl River estuary and decreased rapidly downstream. Anthropogenic sewage input appeared to be an important source of the DOC pool in the upper estuary. DOC distribution was non-conservative during the estuarine mixing, showing a net consumption of DOC in the upper reach and in the low salinity (S<20) region of the Pearl River estuary. Changes in the relative compositions of carbohydrates (MCHO vs. PCHO) and amino acids (DFAA vs. DCAA) along the salinity gradient further indicated that different processes (biodegradation, flocculation, and phytoplankton production) had different influences on distributions of organic compound classes in this estuarine system. Our one-month incubation experiment further revealed that a substantial portion (15-45%) of DOC from the estuary was biodegradable. Bacterial respiration rates were much higher (0.12-5.8 mu mol O-2 L-1 h(-1)) than the DOC consumption rates, suggesting that there were other oxygen consumption processes, such as nitrification besides the aerobic respiration of organic matter in the Pearl River estuary, as inferred by the distribution of NH4+ and NO3-. We estimated that 5.3 x 10(8) g C d(-1) of DOC can be exported out from the Lingdingyang Bay (a major subestuary of the Pearl River estuary) to the continental shelf of the South China Sea during this low flow season. (C) 2009 Elsevier B.V. All rights reserved.Natural Science Foundation of China [40576036, 90711005, 40821063

Constraint of a ruthenium-carbon triple bond to a five-membered ring

含过渡金属碳三键(M≡C)的金属卡拜化合物是许多有机反应的催化剂或关键中间体。对其合成及性质的研究是金属有机化学的热点之一。由于卡拜碳的sp杂化方式，大部分金属卡拜化合物均为链状结构（卡拜碳键角理想值为180 °），环内金属卡拜化合物因存在很大的环张力而难于合成。夏海平教授课题组发展了由链状多炔（称之为碳龙）构筑碳龙配合物的高效方法（Nature Communications, 2017, 8, 1912），实现了锇杂戊搭炔/烯及其衍生物的一锅法合成。现在，他们把该方法进一步拓展到了第二过渡系金属钌，通过碳龙与市售的RuCl2(PPh3)3反应一锅合成了钌杂戊搭炔I。本研究是碳龙化学的进一步延伸和发展，首次把碳龙化学的金属中心由锇拓展到了其它金属，展示了碳龙化学广阔的发展空间。该研究工作在夏海平教授指导下完成，能源材料化学协同创新中心（iChEM）博士后卓庆德和张弘副教授为共同第一作者。iChEM博士后周小茜、博士生陈志昕、林剑锋、卓凯玥、硕士康慧君、林鑫磊参与了部分实验工作。博士生华煜晖负责理论计算。【Abstract】The incorporation of a metal-carbon triple bond into a ring system is challenging because of the linear nature of triple bonds. To date, the synthesis of these complexes has been limited to those containing third-row transition metal centers, namely, osmium and rhenium. We report the synthesis and full characterization of the first cyclic metal carbyne complex with a second-row transition metal center, ruthenapentalyne. It shows a bond angle of 130.2(3)° around the sp-hybridized carbyne carbon, which represents the recorded smallest angle of second-row transition metal carbyne complexes, as it deviates nearly 50° from the original angle (180°). Density functional theory calculations suggest that the inherent aromatic nature of these metallacycles with bent Ru≡C–C moieties enhances their stability. Reactivity studies showed striking observations, such as ambiphilic reactivity, a metal-carbon triple bond shift, and a [2 + 2] cycloaddition reaction with alkyne and cascade cyclization reactions with ambident nucleophiles.This research was supported by the National Key R&D Program of China (2017YFA0204902) and the National Natural Science Foundation of China (nos. 21490573,21332002, and 21561162001). 研究工作得到国家自然科学基金项目（21490573、21332002 和21561162001）和国家重点研发计划（2017YFA0204902）的资助

水溶液中硫酸钾晶体生长动力学

The single crystal growth rates of potassium sulfate in pure aqueous solution under different conditions were determined by photomicrography in a flow system for crystal growth. The effects of themain controlling factors, such as supersaturation, crystal size, solution velocity and crystal growth temperature, on crystal growth rates of potassium sulfate were discussed in detail by using non-linear regression from the experimental data, and several empirical relationships were given. The results showed that the growth rates of crystals increased with supersaturation, crystal size, solution velocity and temperature. Moreover supersaturation was the most important controlling factor influencing growth rates of crystals, crystal size and solution velocity were the secondary and temperature was the least.Furthermore, It was found that the growth rate of crystals along the 100 crystallographic axis was higher than that along the 001 in the same condition. The effect of every factor on crystal growth rates along the 100 crystallographic axis was stronger than that along the 001