茵陈蒿汤防治大鼠非酒精性脂肪性肝炎的实验研究

目的:探索茵陈蒿汤对模型大鼠非酒精性脂肪肝的干预作用。方法:30只雄性Wistar大鼠随机分成正常组、模型组和茵陈蒿汤防治组,模型组和中药防治组给予高胆固醇高脂肪饮食喂养共12周以复制非酒精性脂肪性肝炎模型,中药防治组同时给予茵陈蒿汤灌胃12周。12周后处死大鼠收集标本,检测血清ALT、AST活性、TC含量,肝组织TG含量,对肝脏组织进行HE染色。结果:茵陈蒿汤能有效地减轻大鼠体重和肝重,明显减轻模型大鼠血清炎症损伤的病理状态,改善肝组织脂肪变性的病理状态。结论:茵陈蒿汤能够有效防治非酒精性脂肪性肝炎的病理损伤

交联辛烯基琥珀酸淀粉酯微球对阿司匹林的吸附性能研究

采用反相微乳法制备交联辛烯基琥珀酸淀粉酯微球,利用红外光谱、粒度分析仪、扫描电镜和X射线衍射仪对微球进行表征,研究微球对阿司匹林的吸附性能,采用单因素固定变量法探讨投药量、交联剂用量、反应时间对吸附量的影响。结果表明:原淀粉已成功酯化交联合成变性淀粉微球,微球平均粒径为42.61μm,粒径在80μm以下占93.7%;微球表面粗糙多孔,外观圆整,由原淀粉颗粒的晶体形态变为无定性态;在交联剂用量1.5 g,反应时间3.5 h,投药量22 mg的最佳制备条件下,微球的载药量达到21.48%,包封率达到97.63%,可以作为一种优良的阿司匹林吸附载体

La2O3/γ-Al2O3在柠檬醛与丙酮缩合反应中的催化性能研究

用La2O3负载量不同的La2O3/γ-Al2O3催化柠檬醛和丙酮反应,采用GS/MS、LC/MS、13C NMR等方法鉴定反应产物,并研究了La2O3负载量对反应产物分布、La2O3/γ-Al2O3催化剂的结构性质和酸碱性质的影响.得出以下结论:La2O3是以无定形态负载在γ-Al2O3上;随着La2O3负载量的增加,催化剂比表面积减少,孔径分布趋于均一.La2O3/γ-Al2O3表面增加的弱酸位点密度有利于柠檬醛转化率的提高,增加的中强碱位点密度有利于PSI产率提高;而强碱位点的形成和密度增加对柠檬醛自身缩合生成PCS有促进作用.催化剂循环使用性较好

Simultaneous Determination of Hesperidin and Baicalin in Jian＇er Xiaoshi Oral Liquid by HPLC

建立一种同时测定健儿消食口服液中的橙皮苷和黄芩苷的高效液相色谱法(HPLC).样品用甲醇超声提取,采用Zorbax SB-C18色谱柱上分离,以1%乙酸溶液-乙腈为流动相进行梯度洗脱,检测波长280 nm,柱温为25℃时直接测定.橙皮苷在15.5～155.0μg.ml-1内线性关系良好;黄芩苷在58.0～290.0μg.ml-1内线性关系良好.平均回收率为99.5%和99.6%,RSD为1.1%和0.85%.结果表明,所建立的方法简便、准确,为该制剂的质量控制提供研究依据.To establish a high performance liquid chromatographic （HPLC） method for simultaneous determination of hesperidin and baicalin in Jian＇er Xiaoshi Oral Liquid. The chromatographic separation was achieved on a Zorbax SB--C18 （3.0mm ×250mm, 5μm） column at the detection wavelength of 280nm, using a mobile phase consisted of 1% acetic acid solution and acetonitrile in gradient elution at a flow rate of 0. 35ml.min--1. The linear ranges were 15.5--155.0μg·ml-1 and 58.0--290.0μg·ml-1 for hesperidin and baicalin, respectively. The average recoveries of hesperidin was 99.5% and the relative standard deviations was 1.1% , those of baicalin were 99.6% and 0.85%

Determination of Hesperidin and Arctiin in Ganmao Jieduling Granules by HPLC

建立了HPLC法同时测定感冒解毒灵颗粒中的橙皮苷和牛蒡苷。采用C18色谱柱,以1%乙酸-甲醇(52︰48)为流动相,检测波长280nm。橙皮苷和牛蒡苷在7.5～150和5～100μg/ml浓度范围内线性关系良好,平均回收率为99.2%和99.6%,RSD为0.64%和0.79%。An HPLC method was established for the simultaneous determination of hesperidin and arctiin in Ganmao Jieduling granules. A C18 column was used with the mobile phase of 1% acetic acid-methanol（52︰48） at the detection wavelength of 280 nm. The calibration curves of hesperidin and arctiin were linear in the concentration ranges of 7.5-150 and 5-100 μg/ml. The average recoveries were 99.2% and 99.6%,with RSDs of 0.64% and 0.79%

HPLC-MS/MS法测定四季感冒胶囊中的4种有效成分

建立了高效液相色谱-串联质谱联用法(HPLC-MS/MS)同时测定四季感冒胶囊中升麻苷、橙皮苷、5-O-甲基维斯阿米醇苷和连翘苷4种有效成分含量的方法.4种成分能很好地分离,各成分均有较宽的线性范围和良好的线性关系,回收率为98%~102%.该方法快捷、准确、重复性好,可为四季感冒胶囊的质量控制提供依据.广西民族大学2011年度一般项目(2011MDYB040

A Study on the Properties of Acidolysis Carbonxymethyl Composite Modified Starch Sizing Agent

本文将木薯淀粉进行酸解和羧甲基化反应,制得了酸解羧甲基复合变性淀粉,对其进行了红外表征和浆液、浆膜性能的测试,并通过涤棉高支高密织物的上浆对比试验研究其实际浆纱织造效果。试验结果显示:酸解羧甲基复合变性淀粉属于高浓低粘浆料,粘度热稳定性好,浆膜性能均优于一般变性淀粉,对涤棉粘附性好,浆纱耐磨性、毛羽贴伏性好,织机效率、合格品率高,布品平整柔软,可以作为一种优良的涤棉高支高密织物浆纱材料。 In this paper, the acidolysis carboxymethyl composite modified starch was prepared by acidolysis and carboxymethylation reaction of cassava starch. Its structure was characterized by IR, the size property and sizing film property were tested and the practical sizing and weaving effect were studied through sizing contrast test on polyester/cotton high-count-density fabric. The test results show that the sizing agent has high concentration and low but thermal-stable viscosity, and the size film performance is better than conventional modified starch. It has good adhesion to polyester/cotton fabric, good sizing abrasion resistance and hairiness coverage as well as high loom efficiency and quality rate.广西民族大学校级科研项目(2013MDQN047

Chemical Components of Essential Oils from Chloranthus henryi

目的:分析宽叶金粟兰挥发油的主要化学成分及其抗氧化活性。方法:采用水蒸气蒸馏法获得宽叶金粟兰挥发油,运用气相色谱-质谱联用进行成分分析,同时运用紫外分光光度计测定其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基的清除能力以及总还原能力。结果:从宽叶金粟兰的挥发油中鉴定了21个化合物,占总量的82.30%;挥发油具有较好的还原能力,但对DPPH自由基的清除能力较弱。结论:宽叶金粟兰挥发油主要成分为乙酸冰片酯(45.43%)、3-亚甲基-2-降冰片酮(12.36%)、莰烯(8.74%)等,挥发油具有一定的抗氧化活性。［Abstract］ Objective: This article aimed to study the chemical components of essential oils from Chloranthus henryi and their antioxidant activity． Method: The essential oil was extracted by steam distillation， and its constituents were identified by GC-MS． The antioxidant activity of the essential oil was evaluated on its ability of total reducing and scavenging of 1-diphenyl-2-picrylhydrazy ( DPPH) free radical by spectrophotometry． Ｒesult: Twenty-one compounds，accounting for 82. 30% of the essential oil，were identified． The ability of scavenging DPPH free radical of the essential oils was weak，while the total reducing ability was strong． Conclusion: The main chemical constituents of the essential oil from C． henryi were bornyl acetate ( 45. 43%) ，3- methylene-2-norbornanone ( 12. 36%) and camphene ( 8. 74%) ． And the essential oil showed some antioxidant activity．广西高校科学技术研究项目(YB2014100

Microwave-assisted Extraction of Flavoniods from the Kummerowia striata （ Thunb. ） Schindl

[目的]优化鸡眼草中黄酮类化合物的提取条件。[方法]以鸡眼草的总黄酮提取率为评价指标,通过单因素试验对微波辅助提取鸡眼草黄酮类化合物的工艺条件进行了研究,再运用正交分析法确定最佳提取条件。[结果]微波辅助提取鸡眼草中的黄酮类物质的最佳条件为:乙醇浓度为50%、料液比1∶30(W/V,g/ml,下同)、微波功率300 W,微波处理时间25 min;在此条件下,鸡眼草的总黄酮提取率为3.28%。[结论]微波辅助提取鸡眼草中的黄酮类物质,操作简单易行,提取率较高,是一种理想的提取方法。[-Objectivei To study the optimal conditions for extracting total flavonoids from Kummerowia striata （Thunb）＇Schindl.. [Meth- od] The technological conditions of microwave-assisted extraction of flavoniods were investigated by the single factor and orthogonal experi- ments, and were assessed according to the extraction rate of tatol flavonoids. [ Result] The optimum conditions of microwave-assisted extrac- tion were 50% ethanol as solvent, 1：30 （ W/V, g/ml） of solid-liquid ratio, 300 W of microwave power and 25 min of microwave extraction time. Under the conditions, the extraction rate of total falconoid was 3.28%. [ Conclusion] The microwave-assisted extraction extracting fla- voniods in Kummerowia striata （Thunb.） Schindl. is a simple and ideal extraction method with high efficiency

Determination of Vitamine B_6 by Poly（L-Glutamic acid） Modified Electrode with Flow-injection Irreversible Biamperometry

建立了使用聚合膜修饰电极测定维生素B6的新方法。在pH 6.80的磷酸盐缓冲溶液(PBS)中,用循环伏安法在玻碳电极上制备聚L-谷氨酸修饰膜,并且研究维生素B6在聚L-谷氨酸膜修饰电极上的电化学行为及测定方法,即在外加电压为0条件下流动注射双安培法测定维生素B6的电化学分析方法。在0.1 mol/L PBS(pH 6.80)载液中,维生素B6在聚L-谷氨酸修饰电极上产生一不可逆氧化峰,在选定的最佳试验条件下,流动注射双安培法测定维生素B6氧化峰峰电流与其浓度在4.0×10-6～2.0×10-3mol/L范围内呈线性关系(r=0.993 5,n=10),检出限为9.0×10-7mol/L,连续测定4.00×10-4mol/L维生素B6标准溶液20次,电流值RSD为1.05%,进样频率为120样/h。该方法具有较高的选择性和灵敏度,样品处理方法简单、快速。使用修饰电极建立流动注射双安培法所得线性范围宽,检出限低,可成功应用于西药维生素B6片剂中含量测定,结果令人满意。 A new method for the determination of vitamine B6 was established by using polymer modified electrode in this paper.In pH 6.80 phosphate buffer solution,the poly（L-glutamic acid） modified electrode was prepared by using cyclic voltammetric method on the bare glassy carbon electrode.The voltammetric behavior of vitamine B6 at the poly（L-glutamic acid）modified electrode has been investigated,and flow injection irreversible biamperometry analysis method were studied under the applied potential difference of 0 V to determinate vatamine B6.In 0.1 mol/L（pH 6.80） phosphate buffer solution,a sensitive and irreversible oxidation peak was obtained at the poly（L-glutamic acid）modified electrode.Under the optimum conditions,vitamine B6 can be determined over the range 4.0×10^-6 mol/L-2.0×10^-3 mol/L with the sampling frequency of 120 samples per hour.The detection limit for vitamine B6 is 9.0×10^-7 mol/L and the RSD for 20 replicate determinations of 4.0×10^-4 mol/L vitamine B6 is 1.05%.The method is simple,with high selective rapid and sensitive.The linear range is large,and the detection limit is slow.The method was applied to the determination of vitamine B6 in the drug with satisfactory results.国家自然科学基金项目(21065001);广西民族大学2011年度青年基金项目(2011MDQN03