建立一种同时测定健儿消食口服液中的橙皮苷和黄芩苷的高效液相色谱法(HPLC).样品用甲醇超声提取,采用Zorbax SB-C18色谱柱上分离,以1%乙酸溶液-乙腈为流动相进行梯度洗脱,检测波长280 nm,柱温为25℃时直接测定.橙皮苷在15.5～155.0μg.ml-1内线性关系良好;黄芩苷在58.0～290.0μg.ml-1内线性关系良好.平均回收率为99.5%和99.6%,RSD为1.1%和0.85%.结果表明,所建立的方法简便、准确,为该制剂的质量控制提供研究依据.To establish a high performance liquid chromatographic （HPLC） method for simultaneous determination of hesperidin and baicalin in Jian＇er Xiaoshi Oral Liquid. The chromatographic separation was achieved on a Zorbax SB--C18 （3.0mm ×250mm, 5μm） column at the detection wavelength of 280nm, using a mobile phase consisted of 1% acetic acid solution and acetonitrile in gradient elution at a flow rate of 0. 35ml.min--1. The linear ranges were 15.5--155.0μg·ml-1 and 58.0--290.0μg·ml-1 for hesperidin and baicalin, respectively. The average recoveries of hesperidin was 99.5% and the relative standard deviations was 1.1% , those of baicalin were 99.6% and 0.85%
