The Expression and Significance of TNFAIP8 in Gastric Cancer

胃癌是最常见的消化道恶性肿瘤。虽然胃癌发病的确切机制尚不完全清楚，但环境因素、遗传因素等在胃癌的癌前病变及肿瘤发生发展过程中均起重要作用，尤其免疫调节异常。 肿瘤坏死因子α诱导蛋白8（Tumornecrosisfactor-αinducedprotein-8，TNFAIP8，TIPE）在细胞凋亡、信号传导、肿瘤细胞增生、侵袭及转移等过程中具有重要的调节作用。 为了研究TNFAIP8在胃癌发生发展中的作用及机制，本实验对胃癌病人手术切除的组织进行了TNFAIP8的表达检测，构建了TNFAIP8过表达质粒，以抗DR5单克隆抗体为刺激剂，检测TNFAIP8过表达的人胃癌细胞系BGC823的凋亡及...Gastric cancer is the most common gastrointestinal malignancy. Although the precise pathogenesis of gastric cancer is remains unclear, environmental factors, genetic factors were central to the mechanism of gastric precancerous lesions and tumor development, Tumor necrosis factor-α induced protein- 8 ( TNFAIP8,TIPE) is a re-cently discovered antiapoptotic molecule． Recent data have demonstrated...学位：医学硕士院系专业：医学院临床医学系_外科学学号：2452009115298

The expression of TNFAIP8 in gastric cancer and its clinical significance

目的通过检测胃癌组织中肿瘤坏死因子α诱导蛋白8(TnfAIP8)的表达,探讨其与胃癌临床病理参数的关系及与胃癌发生发展的关系,揭示胃癌的发病机制。方法采用免疫组化SP法,检测50例胃癌以及50例正常胃粘膜中TnfAIP8的表达。结果 50例正常胃粘膜组织中TnfAIP8无表达,而在50例胃癌组织中TnfAIP8阳性表达13例,TnfAIP8阳性率为26%。低分化胃癌组TnfAIP8阳性表达率明显高于中分化腺癌组(P<0.05);Ⅲ期胃癌组TnfAIP8阳性表达率明显高于Ⅱ期胃癌组(P<0.05);有淋巴结转移组TnfAIP8阳性表达明显高于无淋巴结转移组;TnfAIP8在不同性别和年龄的胃癌组织中无差异。结论 TnfAIP8的表达与患者的性别和年龄无关,而与胃癌的组织学分级、胃癌的TnM分期和淋巴结转移有相关性。TnfAIP8在胃癌组织中表达率较正常胃粘膜升高,差异有统计学意义(P<0.05),提示TnfAIP8可能参与了胃癌的发生、发展。Tumor necrosis factor-α induced protein-8(TNFAIP8) is a recently discovered antiapoptotic molecule.Recent data have demonstrated that TNFAIP8 is involved in the regulation of apoptosis,cellular signaling cascade,tumor proliferation and invasion,as well as metastasis.In this study,we aimed to investigate the expression of TNFAIP8 in patients with gastric cancer and their relationships with clinicopathology.We detected the expression of TNFAIP8 in 50 cases of gastric cancer and 50 cases of normal gastric tissues with SP immunohistochemical.In gastric cancer,the expression of TNFAIP8 was located in cytoplasm and/or nucleolus of tumor cells;the expression of TNFAIP8 in patients with gastric cancer(26%) was higher than that in the normal gastric tissues(0%,P﹤0.05).And the expression of TNFAIP8 in poor differentiated adenocarcinoma of gastric was higher compared with that in well differentiated(P < 0.05),the same difference was fund between Ⅲ andⅡ stages of adenocarcinoma.However,the expression of TNFAIP8 was on significant difference among different person with different age and gender.The results suggest that the expression of TNFAIP8 is not related with patient gender or age,but related with the stage of disease.And the expression of TNFAIP8 may be involved in the generation and development of gastric cancer.福建省自然基金(2010D009

羟乙基壳聚糖抗人死亡受体5单链抗体纳米粒的制备、鉴定及体内抑瘤研究

目的为提高抗人死亡受体5单链抗体治疗肝癌的效果,探讨羟乙基壳聚糖抗人死亡受体5单链抗体纳米粒(GCS-aDR5ScFv)的制备、鉴定并研究其对小鼠肝癌H22模型的治疗效果及机制。方法用亲和镍柱层析法纯化抗人死亡受体5单链抗体(aDR5ScFv),采用离子凝胶法制备GCS-aDR5ScFv,应用扫描电镜来检测GCS-aDR5ScFv的外观形态,激光粒度分析仪分析纳米粒的粒径及粒径分布,并检测其表面Zeta电势。建立小鼠肝癌H22模型,用0.272 mg/ml GCS-aDR5ScFv隔天治疗并测量小鼠体质量及肿瘤大小,治疗2周。通过Western blot检测active-caspase8、active-caspase3及BAX表达。结果纯化的aDR5ScFv符合理论条带(Mr30 000),GCS-aDR5ScFv纳米粒形态均一,纳米粒径大小、Zeta电势和多分散指数表明GCS-aDR5ScFv稳定。与正常组相比,治疗组小鼠体质量显著差异,治疗组肿瘤体积和大小差异显著。Western blot检测active-caspase8、active-caspase3、BAX的表达水平上调。结论本研究制备的GCS-aDR5ScFv稳定,对小鼠肝癌H22模型具有抑瘤作用,其机制可能与active-caspase8、active-caspase3及BAX蛋白表达上调相关

LIQUID HOT WATER PRETREATMENT OF Pennisetum hybrid TO ENHANCE SUGAR RECOVERY

The effects of liquid hot water on enzymatic digestibility of Pennisetum hybridare were investigated in this paper. The results indicated that 180 ℃, 40 min, 1 ： 20 ratio of solid to liquid and saturated vapor pressure were the optimal pretreatment condi

COMBINED PRETREATMENT OF SUGARCANE BAGASSE WITH LIQUID HOT WATER AND AQUEOUS AMMONIA TO ENHANCE ENZYMATIC DIGESTIBILITY OF CELLULOSE

A pretreatment combined liquid hot water and aqueous ammonia was investigated to improve the enzymatic digestibility and total sugar recovery of sugarcane bagasse. Compared with single liquid hot water pretreatment, the lignin removal was increased from

高温液态水处理杂交狼尾草提高总糖收率

The effects of liquid hot water on enzymatic digestibility of Pennisetum hybridare were investigated in this paper. The results indicated that 180 ℃, 40 min, 1 ： 20 ratio of solid to liquid and saturated vapor pressure were the optimal pretreatment condi

高温液态水耦合氨法预处理提高甘蔗渣酶解率

A pretreatment combined liquid hot water and aqueous ammonia was investigated to improve the enzymatic digestibility and total sugar recovery of sugarcane bagasse. Compared with single liquid hot water pretreatment, the lignin removal was increased from

高温液态水耦合氨法预处理提高甘蔗渣酶解率

A pretreatment combined liquid hot water and aqueous ammonia was investigated to improve the enzymatic digestibility and total sugar recovery of sugarcane bagasse. Compared with single liquid hot water pretreatment, the lignin removal was increased from