Histochemical study of lectin binding in junctional epithelium of rat

18日-10週齢ウィスター系雄性ラットを用いて臼歯の歯肉・歯肉溝上皮および接合上皮について, その発生過程を含めて, 4種のレクチン, Peanut agglutinin(PNA), Maclura Pomifera agg1utinin(MPA), Wheat-germ agglutinin(WGA), Soybean agglutinin(SBA)を用いて, レクチン組織化学的検索を行った。接合上皮において, 基底細胞はWGA陽性PNA/MPA陰性, 労基底細胞はPNA/MPA/SBA/WGA陽性, 有棘細胞はPNA/MPA陰「生(一部陽性)WGA/SBA陽性, 表層細胞はWGA陽性SBA陰性PNA/MPA陽性であった。基底細胞はWGAのみが陽性反応を示し, 基底細胞細胞表面は基底膜に接する細胞表面のみがWGA陰「生という逆U字型パターンを示し, この特徴は表層細胞にも認められた。歯肉・歯肉溝上皮の基底細胞, 労基底細胞および有棘細胞は接合上皮の各細胞に類似のレクチン染色性を示したが, 接合上皮に比べて有棘層におけるPNA/MPA陽性反応の範囲は狭かった。一次接合上皮は労基底細胞と有棘細胞がPNA弱陽性, MPA陰性, WGA陽性, SBA陽性で, 基底細胞と表層細胞はWGA陽性, SBA陰性であり, 退縮エナメル上皮はすべてのレクチンに対して極めて弱い陽性反応を示した。以上の結果から, 接合上皮の表層細胞は基底細胞に類似のレクチン染色性を示し, その特性は退縮エナメル上皮には認められず, 一次接合上皮において発現される特性であることが示唆された。The present study was designed to analyze the expression of carbohydrate residues of junctional epithelium as compared with those of gingival, primary junctional, and reduced enamel epithelia in rats. In the junctional epithelium, basal cells were Wheat-germ agglutinin(WGA) -posi-tive but Peanut agglutinin(PNA) /Maclura Pomifera agglutinin(MPA) /Soybean agglutinin(SBA) -negative. Since the basal cell surface was WGA-negative and the lateral and apical cell surfaces were WGA-positive, the WGA binding of basal cells appeared to be U-shaped. Parabasal cells were PNA/MPA/WGA/SBA-positive. Spinous cells were PNA/MPA-positive and WGA/SBA-positive. The lectin binding pattern of superficial cells was similar to that of basal cells. The lectin binding pattern of gingival epithelial cells was similar to that of junctional epithelial cells ; however, spinous gingival epithelial cells were virtually PNA/MPA-negative. In the primary junctional epithelium, spinous cells were PNA-positive, MPA-negative, and WGA/SBA-positive, and both basal and superficial cells were WGA-positive and SBA-negative. All cells of reduced enamel epithelium were PNA/MPA/WGA/SBA-slightly positive. Our results suggest that superficial cells of junctional epithelium contain carbohydrate residues similar to those found in basal cells of junctional and gingival epithelia. A Iectin-binding pattern appears in the superficial cells of the primary junctional epithelium, but does not appear in reduced enamel epithelium

三叉神経帯状疱疹における皮膚, 粘膜, 歯槽骨所見

In reviewing lesions and complications of herpes zoster of trigeminal nerve, these appear to be different clinical features and courses of the vesicles in skin and mucous membrane, and complications depend upon the amount of the vesicles. Unusual features of tooth exfoliation and osteonecrosis have also occasionally been reported. We present three cases of severe herpes zoster involving the trigeminal nerve, especially on the courses of lesions of vesicles and herpes zoster infection-related alveolar bone necrosis. The etiology of herpes zoster infection associated with destructive oral sequelae is also discussed

グローバル人材として生徒に育成したい資質・能力の設定と実践 : 総合的な探究の時間の振り返りシートを手がかりに

Ⅲ大学以外の授業実践研