Optimized Preparation Method of Pseudovirus for Human Papillomaviruses and Establishment of a High-throughput Neutralization Assay

人乳头瘤病毒（HPV）能够引起包括宫颈癌在内的多种生殖道疾病，严重危害人类健康。HPV具有严格的宿主特异性和组织分化依赖性，难以通过常规组织培养方法扩增，也难以从病变组织中分离获得足够的病毒。因此建立简便、高效的HPV体外感染模型是发展HPV预防疫苗与治疗药物的迫切需要。近来研究发现HPV假病毒可用于在体外有效模拟HPV的感染，以此基础建立的中和实验方法具有较突出准确、便捷的优点，在HPV预防疫苗、中和抗体研究中显示出巨大的应用潜力。HPV假病毒中和实验方法的广泛使用需要进一步提高假病毒构建效率，同时能够满足高通量检测的需要。本实验室已应用磷酸钙多质粒共转染方法在293FT细胞中建立了构建HP...Infection of Human papillomavirus (HPV) is the cause of various diseases of genital tract, including cervix cancer, which is the second most common cancer leading to death in women. HPV’s infection has host specificity, moreover, completed life cycle of HPV relays on differentiation of epithelial cells.Absence of applicable cell culture systems for infectious virus and limited virus particles obta...学位：理学硕士院系专业：生命科学学院生物学系_细胞生物学学号：2012005130205

无模型隐含波动率及其所包含的信息:基于恒生指数期权的经验分析

根据无模型隐含波动率方法,对香港恒生指数期权所含信息进行研究,并通过使用无模型隐含波动率对期权市场的效率进行直接检验,结果发现:无模型隐含波动率所含信息最多,它完全包含了所有历史波动率所含信息,香港恒指期权市场是有效的;在对未来一个月的预测中,无模型隐含波动率还完全包含了BS隐含波动率的信息,在对未来两个月的预测中,无模型隐含波动率虽不能完全包含BS隐含波动率,但仍然包含了最多的信息;期权市场交易量的大小,同时交易的不同行权价的期权的多少,是影响无模型隐含波动率预测能力的重要因素;为追求积分密度进行过多人为的插值以及过大区间的积分,会导致无模型隐含波动率预测能力的降低,由此得到了无模型隐含波动率的相对合适的计算方式.美国已于2003年开始采用无模型隐含波动率编制波动率指数.随着金融衍生品市场的不断发展,无模型隐含波动率在资产定价、风险管理方面将发挥越来越重要的作用

PIV study on spray field of coal-water slurry air-blast electrostatic atomization

为探索高压静电场中水煤浆荷电颗粒相的动力学特性,基于新型三级y型水煤浆气力式雾化喷嘴-环电极的静电雾化实验系统,利用粒子图像速度仪(PIV)对雾炬内水煤浆荷电雾滴的运动规律进行了深入的实验研究。研究结果表明:在射流边缘区域内的水煤浆荷电细小雾滴群在静电场力的作用下,沿程将偏离常规雾化的运动轨迹,靠近电极处的流线发生明显的弯曲变形并随电压的不断增强呈现出3种典型形式;荷电雾滴的轴向速度随电场力增强而减小,并在射流核心区内的沿程截面上分布趋于均匀,径向速度随电场力的增强而增加,并在射流核心区内的沿程截面上呈线性分布;流场中雷诺应力及涡量均集中分布在射流轴线两侧,随电场增强,雷诺应力峰值显著增加并在横截面上逐渐外移,随射流远离喷嘴,其截面雷诺应力分布趋于平缓。In order to explore dynamic characteristics of coal water slurry charged particle in the high voltage electrostatic field,using particle image velocimeter( PIV),an electrostatic atomization experiment system consisting of a novel three-level Y-type spraying-pneumatic atomizing nozzles and a ring electrode was applied to perform an in-depth study on the dynamic characteristics of charged coal-water slurry( CWS) droplets in a high voltage electrostatic field.Experimental results indicate that: the trajectory of fine coal-water slurry droplets clusters at the edge area of the spray field deviated from conventional atomization under the action of static electric force.The streamlines near the electrode obviously bend and deform,which would present three typical forms with the increasing voltage.The axial velocity of charged droplets of coal-water slurry would decrease and distribute more evenly while the radial velocity would increase and distribute linearly along the spraying developing sections of radial direction with the increasing electrostatic force.Reynolds stress and vortices in the flow field would be concentrated in the spray axis on both sides,the peak value of Reynolds stress would increase significantly and gradually move out on the cross with the electric field enhanced.Reynolds stress would distribute more smoothly as the spray developing away from the nozzle.福建省科技计划重点资助项目(2012H0031); 福建省科技厅高校专项资助项目(JK2011028

Volatility Forecast:GARCH Model vs Implied Volatility

在预测未来波动率时,究竟是基于历史数据的时间序列模型还是基于期权价格的隐含波动率模型效率更高?本文对香港恒生指数期权市场所含信息的研究发现,在预测期限较短(一周)时,GARCH(1,1)模型所含信息较多,预测能力最强,但在预测较长期限(一个月)时,隐含波动率所含信息较多,预测能力较强。同时,期权市场交易越活跃,所反映的信息就越全面,隐含波动率的预测能力也就越强。国家自然科学基金面上项目:“非完美信息下基于观点偏差调整的资产定价”(70971114);教育部“国际金融危机应对研究”应急项目:“金融市场的信息功能与金融危机预警”(2009JYJR051);福建省自然科学基金:“卖空交易对证券市场的影响研究”(2009J01316

Photosynthetic capacity of three common species of macroalgae and the application in coral aquarium

利用水下饱和脉冲调制叶绿素荧光仪(dIVIng-PAM)测定了珊瑚养殖水处理中3种常见大型海藻石莼(ulVA lACTuCA)、条浒苔(EnTErOMOrPHA ClATHrATA)和羽状蕨藻(CAulErPA MEXICAnA)光能转化效率(fV/fM),快速光曲线(rlC)相关参数和非光化学淬灭(nPQ)。结果表明:石莼和条浒苔的fV/fM分别为0.808±0.004和0.816±0.009,显著高于羽状蕨藻。最大相对电子传递速率rETrMAX(17.52±2.92和19.59±4.43μMOl E·M-2·S-1)、半饱和光强Ik(53.41±8.18和59.71±13.52μMOl PHOTOnS·M-2·S-1)也明显高于羽状蕨藻(rETrMAX和Ik分别为13.72±5.41μMOl E·M-2·S-1和32.67±14.06μMOl PHOTOnS·M-2·S-1),表明石莼和条浒苔有着比羽状蕨藻更高的光合能力和对强光的耐受能力,光保护能力更强;羽状蕨藻较高的rlC初始斜率α表明其在弱光下拥有更强的光捕获能力,弱光下光合效率更高;随光照的增强(0~373μMOl PHOTOnS·M-2·S-1),羽状蕨藻nPQ的增幅有限(0~0.2),最大值仅为条浒苔和石莼的38.1%和62.5%,表明这种藻类更容易受到强光的抑制。因此,在珊瑚养殖的水处理中,可以根据不同的光照选择适宜的藻种或藻种组合并根据光梯度进行布局,反之也可以根据藻种来选择合适的光源,以达到最佳的处理效率。This study investigated the difference of photosynthetic capacity in three macroalgae species( Ulva lactuca,Enteromorpha clathrata,and Caulerpa mexicana) commonly used for nutrient removal in coral aquariums.The photochemical efficiency( Fv/Fm),parameters relevant to rapid light curve( RLC) and non-photochemical quenching( NPQ) in the macroalgae were measured by underwater saturation pulse modulated chlorophyll fluorometer( Diving-PAM).The results showed that Fv/Fmwas 0.808 ± 0.004 for U.lactuca and 0.816 ± 0.009 for E.clathrata,which were higher than that for C.mexicana.Higher maximum relative electron transfer rates( rETRmax)( 17.52 ±2.92 and 19.59 ±4.43 μmol e·m- 2·s- 1) and half-starved value of light intensities( Ik)( 53.41 ±8.18 and 59.71 ±13.52 μmol photons·m- 2·s- 1) were observed in U.lactuca and E.clathrata than that in C.mexicana( 13.72 ± 5.41 μmol e·m- 2·s- 1for rETRmaxand 32.67 ± 14.06 μmol photons·m- 2·s- 1for Ik),indicating that U.lactuca and E.clathrata have stronger photosynthetic capacities,photo-protection capacities and tolerance to higher intense light than C.mexicana.A significantly lower RLC initial slope α in C.mexicana implied that this algal species has a higher photosynthetic capacity under weak light condition.With increasing the light intensity( 0- 373 μmol photons·m- 2·s- 1),however,C.mexicana had a limited increase of NPQ( 0- 0.2),the maximum of which was equivalent to 38.1% of that in U.lactuca and 62.5% of that in E.clathrata.These results indicate that the photosynthesis of E.clathrata may be more easily limited by intense light.Our study suggests that the selection of algal species for nutrient removal in a coral aquarium should be adapted to the light intensity.Single species or the combination of different species of macroalgae can be selected according to the illumination of the coral aquarium in order to maximize the efficiency of nutrient removal.国家海洋局第三海洋研究所基本科研业务项目(海三科2011033); 厦门海洋研究开发院共建项目; 海洋公益性行业科研项目(201105012)资

铁对中肋骨条藻生长、色素化程度及氮同化能力的影响

以中肋骨条藻为对象，研究了铁以及铁与氮的交互作用对藻类生长、色素化程度及氮同化能力的影响。结果表明，在3x10￣8~5x10-6MOl/dM3浓度范围内，随着fE的增加，该藻对氮的吸收量增加，同化能力增大，细胞色素化程度降低，胞内氨基酸（AA）和蛋白质（Pr）含量升高，细胞生长加快，细胞生长率在fE浓度为5x10-6MOl/dM3时达最大。叶绿素A和褐色素以及胞内AA和Pr与铁浓度的对数呈正相关关系。实验还发现，当n/P为20：1时（比较40：1和10：1），铁与氮交互作用对细胞生长、色素化程度以及氮同化能力影响尤其显著：当fE浓度一定时，不同形态的氮源对藻胞内AA转化为Pr速率的影响是低氧化态氮源高于高氧化态氮

高超声速飞行器一体化方法研究

针对高超声速飞行器一体化设计方法现状的分析,阐明了吸气式发动机与乘波体飞行器之间高效的一体化对于高超声速飞行的重要作用,并从理论、原理、设计方法 3方面进行介绍。在激波理论方面,通过从直线激波的求解拓展到二次曲面激波的求解,为3维曲面激波的研究提供了帮助;在乘波原理方面,将乘波原理从外流乘波拓展到内流乘波,继而提出1种兼顾内外流需求的双乘波原理,深化了乘波原理的内涵;在设计方法方面,对于基本流场的气动设计问题,提出更加高效的一体化气动反设计方法。综上分析并归纳出准3维内外流一体化乘波理论与方法,从而在现有"准3维"研究体系上,构建并完善了全3维内外流一体化乘波理论与方法,对于复杂3维超声速内外流一体化设计技术的发展具有一定借鉴作用。中央高校基本科研业务费专项资金(20720170055)福建省自然科学基金(2016J06011)装发预研领域基金(61402060301)航空动力基金(6141B090308)兵发预研基金(6141B010266)资

一个包含HCVIRES的高效真核双顺反子表达载体的构建

In this paper, a new eukaryotic bi-cistronic expression vector containing Hepatitis C Virus(HCV) internal ribosome entry site (IRES) expressing two foreign genes from one mRNA was constructed. The sequence starting from the 5' untranslated region of 18nt to 32nt in HCV core coding region was cloned and then the encephalomyocarditis virus (ECMV) IRES sequence in the commercial vector pIRES was substituted to construct a new vector pCVIR. Green fluorescent protein (GFP) and Hepatitis B virus surface antigen (HBsAg) coding genes were inserted up-stream and down-stream of IRES sequence. The fluorescence intensity of GFP and HBsAg were determined by flow cytometry and ELISA respectively., thus, the expression efficiency of the two vectors, pCVIR and pIRES could be compared. The experimental results showed that the vector pCVIR could translate the GFP and HBsAg genes down-stream of its HCV IRES sequence more efficiently without impairing the expression of genes up-stream of IRES sequence than the vector pIRES.It is..

戊型肝炎病毒重组颗粒性蛋白疫苗在小鼠体内诱导的免疫应答研究

HEV 239是福建省医学分子病毒学研究中心实验室研制的一种戊型肝炎病毒(HEV)重组颗粒性蛋白疫苗,该文旨在研究HEV239蛋白疫苗在小鼠体内诱导产生特异性免疫应答的情况。将5μg HEV 239蛋白疫苗(239-Pro)、加铝佐剂疫苗(239-Vac)或加弗氏佐剂疫苗(239-CFA)肌肉注射免疫BALB/c鼠3次,第8周检测鼠血清抗HEV抗体及其亚类,同时用ELISPOT方法检测细胞毒性T细胞(CTL)应答。结果显示:239-Vac诱导的抗体滴度与239-CFA相当,高于无佐剂的239-Pro。239-Vac诱导的抗体中,IgG1/IgG2a比值显著高于239-CFA和239-Pro,主要为Th2型应答。除239-CFA之外,239-Vac和239-Pro也可诱导出一定的HEV抗原特异性I型Tc应答。提示:重组抗原HEV 239能诱导良好的抗体应答及一定的Tc1应答

重组慢病毒对不同哺乳动物细胞基因转移及表达效率的研究

慢病毒是一种具有独特优点和巨大应用潜力的哺乳动物细胞基因转移载体,我们对慢病毒载体对不同哺乳动物细胞的基因转移及表达效率进行了平行比较研究.应用第三代重组慢病毒系统构建了携带CMV启动子-EGFP报告基因表达元件的重组慢病毒Lenti-EGFP,分别对多种不同哺乳动物细胞进行转导实验,在转导48 h后应用流式细胞仪检测报告基因在不同细胞株中的转移及表达效率.我们共使用了29种哺乳动物细胞株,包括14种人类组织细胞,5种猴组织细胞,9种鼠组织细胞,1种兔组织细胞.结果显示,重组慢病毒具有良好的基因转移能力,可有效进入多数哺乳动物细胞,对不同种属来源的细胞没有表现出特别的偏嗜性,但对贴壁培养细胞的基因转移效率明显高于对悬浮培养细胞.本研究为重组慢病毒系统的合理使用提供了基础