Pylogenetic analysis of bacterioplankton and bacteria in surface sediments from the Northern Bering Sea

1．通过PCR-DGGE分析，发现白令海北部底、表层海水中的细菌生物多样性水平总体较高，而且底层水体中细菌多样性指数及丰度明显高于表层水体；St.Lawrence岛北部7个站位的表层和底层水样，其丰度和多样性指数平均值低于南部区域；采样深度大于100m的3个站位的底层水样，其多样性指数随着深度的增加出现递增现象。获得的36条DGGE条带，其序列除了有2条属于环境样品中的未知菌群以外，涵盖了以Proteobacteria和Actinobacteria为主的6个门。其中，gamma-Proteobacteria和alpha-Proteobacterium分别以25.0%和22.2%占据优势地位。另...Biodiversity of bacteria in the bottom and surface water, as well as the bacteria in the top layer of sediments from the Northern Bering Sea, were studied by a combination of PCR-DGGE, RFLP and 16S rDNA analysis. Shannon-wiener’s Index and software BandScan were used to evaluate the bacterial biodiversity. The main results were as follows: 1. DGGE analysis showed that the bacterial biodive...学位：理学硕士院系专业：生命科学学院生物学系_微生物学学号：2172006115216

白令海北部表层沉积物中细菌多样性的研究

提取白令海表层沉积物样品总DNA,构建沉积物中的细菌16SrDNA文库,通过RFLP酶切分型与序列测定,进行沉积物中的细菌多样性及系统发育分析。结果表明,该沉积物中细菌多样性丰富,获得的125条序列归属于10个细菌类群,包括占据优势地位的变形细菌(Pro-teobacteria,49.6%)、酸杆菌门(Acidobacteria,20.8%),以及拟杆菌门(Bacteroidetes,6.4%)、放线菌门(Actinobacteria,5.6%)、浮霉菌门(Planctomycetes,5.6%)、硝化螺旋菌门(Nitrospirae,4%)、疣微菌门(Verrucomicrobia,2.4%)、绿弯菌门(Chloroflexi,1.6%)、纤维杆菌门(Fibrobacteres,0.8%)、螺旋体(Spirochaetes,0.8%)。此外,还有一部分分类地位尚不明确的细菌(2.4%)。而在变形细菌中,δ、γ亚群为其中的优势类群

Species composition and ecological distribution of planktonic diatoms in the Changjiang River estuary during Spring

20 0 2年春季 ,在长江口附近海域典型赤潮高发区 2 8个大面站位采集了 5 3个样品 ,从中共鉴定出隶属于 31个硅藻属的 80个种和变种 ;其中种类多样性较高的属为圆筛藻属 (Coscinodiscus) ,有 17个种 ,斜纹藻属 (Pleurosigma) ,有 8个种和变种 ;数量上较优势的种为柔弱拟菱形藻 (Pseudo nitzschiadeli catissma) ,为 3.4 8× 10 3 cells·L-1,占 2 8.5 4 % ;具槽直链藻 (Melosirasulcata) ,为 1.4 3× 10 3 cells·L-1,占16 .98% ;尖刺拟菱形藻 (Pseudo nitzschiapungens) ,为 0 .71× 10 3 cells·L-1,占 9.85 % .它们在大部分站位中都有出现 ;柔弱拟菱形藻和尖刺拟菱形藻的高细胞密度区主要出现在 12 30E断面的站位 ,而具槽直链藻则主要出现在长江口的 31～ 32°N断面的站位 .浮游硅藻总细胞丰度变化于 0 .4 3× 10 3 ～ 2 3.3× 10 3 cells·L-1,平均 4 .6 1× 10 3 c... 【英文摘要】 water samples were collected from 28 stations in typical regions where redtide frequently occurred in the Changjiang River estuary during Spring, 2002. In total, 80 diatom species and varieties belonging to 31 genera were identified, among which, genera with high species biodiversity included Coscinodiscus which contained 17 species and Pleurosigma which contained 8 species and varieties. Quantitative analysis of diatom cell density showed that Pseudo-nitzschia delicatissma (3.48×10 3cells·L -1, 28.54%...国家重点基础研究发展规划资助项目 ( 2 0 0 1CB40 970 1)

Pose Error Analyses of the Stewart Platform

从并联机构与串联机构的运动学等效 ,并联机构本身特征与并联机构实际工作空间出发 ,考虑各分支末端误差对最终运动平台末端误差的影响 ,提出了并联机构位姿误差放大因子分析法·依据位姿误差放大因子具有对误差定量分析的特点 ,该分析方法既可用于机构参数优化 ,又可用于结构精度设计· 最后 ,给出了一个实例说明本方法的有效性&middot

Polar microorganisms,a potential source for new natural medicines-A review

通讯作者。Tel: +86-592-2183217; Fax: +86-592-2184528; E-mail: [email protected] 作者简介: 曾胤新(1971− ), 男, 重庆人, 在职博士研究生, 副研究员, 研究方向为极地及海洋微生物学。E-mail: [email protected][中文文摘] 微生物是极地生态系统的重要组成部分。由于极地独特的地理、气候及环境特点,极地微生物所具有的新颖性与多样性,在科学研究、应用开发等方面都具有重要价值,并逐渐受到人们的广泛关注。有关极地微生物的资源勘探与代谢活性产物研究,已成为国际微生物学研究的热点之一。结合笔者的工作实践,对近年来极地微生物资源的勘探与收集情况进行了介绍,并着重对以医药与生物农药等为目标的极地微生物产活性化合物的研究现状进行了概述,同时还对目前一些影响研究工作开展的限制因素进行了讨论。可以预见,针对极地微生物资源的研究开发工作,将会给新型天然药物的筛选与发现带来新的机遇与突破。[英文文摘] Microorganisms are an important component of polar ecosystems.Based on the unique features of geographical location,climate and environment of polar regions,the novelty and biodiversity of polar microorganisms have attracted an increasing attention for their significant values in both scientific research and resources exploitation.Bioprospecting and natural products research of polar microorganisms have become a hot field of microbiology.In this review,following the introduction of bioprospecting and culture collection of polar microorganisms in recent years,the research and develop-ment of microbial bioactive compounds aimed for pharmaceuticals and biological agriculture chemicals are summarized.In addition,factors that currently limit the research progress are discussed.It is believed that the resources exploitation of polar microorganisms can provide an opportunity for discovery of new natural medicines.Compared to human pharma-ceuticals,the research and development of biological agriculture chemicals has more possibilities to achieve a break-through in a short period of time.国家自然科学基金(40676002);国家“973项目”(2004CB719601

Novel algorithm of fast extrication for mismatch in tailored welded blanks

本文讨论了基于视觉和结构光的焊缝形貌视觉检测系统的组成原理,针对拼板激光焊接工程中对焊缝检测的实时性要求高的特点,创新性地提出了一种快速获得焊缝错配缺陷的算法,主要步骤为:首先基于有关标准的检测指标阈值的设定,再次,针对在线提取到的图像进行预处理,主要是加窗和中值滤波;最后为Radon变换与错配检测。该算法能减少计算任务,从而快速获得错配检测指标。实验给出了不等厚板拼焊时的线性错配的数值和分布,同时验证了该算法的有效性

The Kinematics of the Parallel Manipulators with the Derived Motion Structure

根据运动学等效的原则，在并联机器人中引入等效串联机器人及分支等效串联机器人，以等效广义坐标为中间变量建立并联机器人运动学正道解求解算法。该算法能有效处理结构带来的运动耦合，并且规划的软件具有自动生成迭代初始点、避免多解性以及便于实际应用等特点，从而为并联机器人的结构设计与创新提供了理论支持

Remote control director for working dog

本发明涉及一种工作犬遥控指挥仪,它由微型计算机、无线数传设备、定位设备、耳机、无线图像发射机、无线图像接收机、计算机组成,其中微型计算机为核心器件,通过音频接口与耳机连接;通过串口分别与定位设备和无线数传设备接口;摄像头直接与无线图像发射机相连;无线图像接受机通过图像采集卡将图像信号送入计算机;计算机是能够以无线或有线方式连接Internet的计算机;无线图像传输设备由无线摄像头或摄像头加无线发射机,无线接收机组成。微型计算机应具有音频接口。电池为计算机和无线图像接收机以外的其它设备供电。采用本发明可实现工作犬的视觉反馈,定位跟踪,可用于在训导员和指挥员不可见范围内动态指挥工作犬独立完成警务作业

On-line quality inspection in laser blank welding using ART2 neural network

Laser blank welding is becoming more and more important in the automotive industry and the quality of the weld is critical for a successful application.A fully automated solution is required to inspect the quality of the blanks.This paper presents a vision inspection system with a CMOS camera which uses ART2 network to inspect the defects on-line to obtain the geometry and the quality of the weld seam.The neural network ART2 has the capability of self-learning from the environment. It can distinguish the defects that have been learned before and give new outputs for new defects.So ART2 network is suitable for weld quality inspection in laser blank welding.Additionally,a CO_2 laser is used for the blank butt-welding