长汀县赢坪史前遗址发掘简报

2012年福建博物院对赢坪两处遗址进行了抢救性考古发掘,获得了一批包括石器、陶器、铜器、石范等内涵丰富的文化遗物,这批遗物可进一步了解闽西地区的史前文化发展序列及文化面貌,并且对于多地区文化间的交流研究也有一定的作用

一种硝化苯硼酸亲和色谱预柱及其制备和应用

本发明涉及亲和色谱柱，具体地说是一种硝化苯硼酸亲和色谱预柱及其制备和应用；预柱填料为硅胶基硝化苯硼酸，用于HPLC在线富集临顺二羟基化合物。本发明预柱除了具有的耐压和不溶涨的特点外，较未硝化苯硼酸功能基团对应的亲和色谱预柱具有保留强度大和保留适用 pH宽等优点，非常适于两柱联用的HPLC在线分析系统，定性定量结果好。带填

基于液相色谱质谱的代谢组学方法在疾病标志物发现中的应用

Metabonomics is an important platform of systems biology, which focuses on the dynamic metabolic changes in the organism. When applied to life science, metabonomics surveys provide great potential in the exploration of potential biomarkers, which could improve diagnosis and subsequent clinical outcome. Based on the chromatography/mass spectrometry analytical techniques, we have developed an integrated strategy for the metabonomic study of diseases. Firstly, we had set a standard for sample collection which may ensure the data quality for the whole study. Secondly, an effective method for the treatment of massive data was developed based on chemometrics and feature selection techniques. Thirdly, a method for the structure identification of unknown metabolites has also been established. The last part is the biological interpretation and validation of the potential biomarkers. In our group, the metabonomic platform had been applied to the study of cancer, diabetes 2 and other diseases. In this presentation, I would give an introduction of our metabonomic platform and its application on the study of cancer and diabetes.Metabonomics is an important platform of systems biology, which focuses on the dynamic metabolic changes in the organism. When applied to life science, metabonomics surveys provide great potential in the exploration of potential biomarkers, which could improve diagnosis and subsequent clinical outcome. Based on the chromatography/mass spectrometry analytical techniques, we have developed an integrated strategy for the metabonomic study of diseases. Firstly, we had set a standard for sample collection which may ensure the data quality for the whole study. Secondly, an effective method for the treatment of massive data was developed based on chemometrics and feature selection techniques. Thirdly, a method for the structure identification of unknown metabolites has also been established. The last part is the biological interpretation and validation of the potential biomarkers. In our group, the metabonomic platform had been applied to the study of cancer, diabetes 2 and other diseases. In this presentation, I would give an introduction of our metabonomic platform and its application on the study of cancer and diabetes

Metabolomic identification of potential biomarkers for acute graft rejection of renal transplantation by using RPLC and HILIC coupled with mass spectrometry

Acute graft rejection is a major post-transplant complications of renal transplantation, the success of renal transplantation is closely associated with the ability to control rejection. Therefore, early detection and accurate diagnosis are critical in the management of patients receiving renal transplantation. Metabonomics is the identification and measurement of metabolic profile dynamics of living systems to toxin or drug, environmental changes and diseases. As a powerful analytical platform, the application of metabonomics has increased in therapy and novel potential biomarker discovery. In our study, a metabonomics technique based on ultra performance liquid chromatography (UPLC) coupled with Q-TOF mass spectrometry was employed to investigate plasma metabolic profile of acute renal graft rejection patients in order to find potential disease biomarkers and to reveal its pathophysiological changes. The reversed-phase chromatography and hydrophilic interaction chromatography were combined to achieve more comprehensive metabolism. Creanitine and indoxyl sulfate which are clinical markers of renal injury were also found as potential discriminating metabolites in our study. Moreover, phospholipids- metabolism and FFA- metabolism alterations were identified in acute graft rejection of renal transplantation patients. These results suggest that combined LC-MS metabonomic approach is applicable to investigate the metabolic abnormality in the acute graft rejection of renal transplantation rats. These potential biomarkers may be useful molecules for the clinic in either diagnosis or therapy of acute graft rejection.Acute graft rejection is a major post-transplant complications of renal transplantation, the success of renal transplantation is closely associated with the ability to control rejection. Therefore, early detection and accurate diagnosis are critical in the management of patients receiving renal transplantation. Metabonomics is the identification and measurement of metabolic profile dynamics of living systems to toxin or drug, environmental changes and diseases. As a powerful analytical platform, the application of metabonomics has increased in therapy and novel potential biomarker discovery. In our study, a metabonomics technique based on ultra performance liquid chromatography (UPLC) coupled with Q-TOF mass spectrometry was employed to investigate plasma metabolic profile of acute renal graft rejection patients in order to find potential disease biomarkers and to reveal its pathophysiological changes. The reversed-phase chromatography and hydrophilic interaction chromatography were combined to achieve more comprehensive metabolism. Creanitine and indoxyl sulfate which are clinical markers of renal injury were also found as potential discriminating metabolites in our study. Moreover, phospholipids- metabolism and FFA- metabolism alterations were identified in acute graft rejection of renal transplantation patients. These results suggest that combined LC-MS metabonomic approach is applicable to investigate the metabolic abnormality in the acute graft rejection of renal transplantation rats. These potential biomarkers may be useful molecules for the clinic in either diagnosis or therapy of acute graft rejection

全二维气相色谱技术及其进展

许多分析问题的解决需要得么比一维色谱技术能提供的更高的分辨率，分离能力可通过使用多种分离技术或机理的组合来增强。此时，样品被分散在不同的时间维，最终的分辨率强烈地依赖珩这些维间分离特性的差异。当它们之间没有关联，也取相互间正交时，系统可获得最高的分辨率。全二维气相色谱（GC×GC）提供了一个真正的正交分离系统。它把分离机理不同而又相独立的两支色谱柱以串联方式结合组成二维气相色谱。在这两支色谱柱之间装有的一个调制器起捕集再传送的作用。全二维色谱的峰容量为组成它的两支色谱各自峰容量的乘积。介绍了GC×GC的技术和仪器，比较了现阶段GC×GC系统的3种设计。列举了在石化和环保领域GC×GC的许多应用实例，简单地讨论了GC×GC将来的发展前景