痛风性关节炎骨质破坏中医证型分布特点与相关因素分析

目的总结痛风性关节炎伴或不伴骨质破坏与中医证型分布特点及实验室指标的相关性,以期为痛风性关节炎伴骨质破坏患者的临床治疗提供可靠依据。方法纳入2017年9月至2018年9月在黑龙江中医药大学附属第一医院风湿免疫科门诊及住院治疗且行X线诊断的痛风性关节炎患者的临床资料进行回顾性分析,根据X线诊断分为痛风性关节炎伴骨质破坏组(A组)52例和痛风性关节炎不伴骨质破坏组(B组)48例,归纳两组患者中医证型及实验室检查,所有数据输入Excel表格,采用SPSS22.0进行统计分析。结果痛风性关节炎伴或不伴骨质破坏患者一般情况差异无统计学意义(P> 0.05)。痛风性关节炎伴或不伴骨质破坏患者血糖、血脂、肌酐及尿酸差异有统计学意义(P 0.05)。痛风性关节炎伴或不伴骨质破坏患者中医证型分布差异有统计学意义(P <0.05)。其中瘀热阻滞证、痰浊阻滞证与痛风性关节炎伴骨质破坏关系最为密切,有统计学意义(均P <0.01)。结论痛风性关节炎伴骨质破坏患者的血糖、血脂、肌酐与尿酸的代谢水平普遍较痛风性关节炎不伴骨质破坏患者差,中医证型以瘀热阻滞证及痰浊阻滞证为主,并且与其关系最为密切。黑龙江省博士后科研启动金资助项目(LBH-Q12011

N-(2-羟基乙基)芥酰胺对高脂血症金黄地鼠的降脂及保肝作用

高脂血症是诱发心血管疾病的主要危险因素之一,控制血脂的升高对于预防心血管疾病具有十分重要的意义。对于多数患者来说,单纯通过改善饮食结构来严格控制血脂水平是比较困难的,通常需要借助药物进行治疗。目前临床上广泛使用的降血脂药物主要包括HMG-COA还原酶抑制剂、贝特类等。研究表明,过氧化物酶体增殖激活受体α(PPARα)激动剂具有调血脂功能,其合成配体贝特类已成为治疗高脂血症的一线药物,但长期应用易引起肝

运用调和营卫法治疗产后痹浅析

产后痹是指妇女在产褥期或产后,出现肢体疼痛、酸楚、麻木、重着、畏风怕冷、以及关节活动不利等症状的疾病。古今医家在不断探索实践的过程中,对产后痹的认识各有独到的见解。调和营卫法是通过发表解肌的方法,调整机体营卫失衡的状态使其恢复营卫调和平衡的一种治疗方法。营卫不和可致机体正气亏虚,风寒湿邪极易乘虚而入,使外邪留著营卫,致营卫失和,发为本病。营卫不和为产后痹病因的基础,调和营卫法则为产后痹的治法总则。本文主要阐述产后痹关键在于营卫不和的机理,论证调和营卫法是治疗产后痹的重要大法。在临床上应用调和营卫法,以桂枝新加汤为基础,临床上随证加减,疗效确切

让城市总体规划更有用

今天这场学术对话的主题是\"让城市总体规划更有用\"。城市总体规划是一个城市全局性、综合性规划,在统筹城乡发展、优化空间开发格局、合理配置空间资源、保障公共利益等方面发挥着战略引领和刚性控制作用,是城市重要的公共政策和指导城市建设的法定依据。无论从城乡规划法律的制度设计还是从城市总体规划的属性来看

高粱子粒淀粉表面色素的树脂分离研究

对纯化高粱淀粉过程中所得到的淀粉共生天然色素进行了树脂分离纯化研究。得到了树脂分离高粱淀粉共生色素的吸附动力学结果,3 种大孔吸附树脂 HPD-600、AB-8、H103 对高粱红的吸附为慢速平衡型。通过对树脂分离所得色素成份的红外光谱、紫外光谱、质谱分析得出,高粱籽粒淀粉共生色素与高粱壳中的高粱红主要成份是同样的物质,鉴定出所分离的主要成份之一为 5,7,4′-三羟基黄酮

BMP2 gene delivery mediated by chitosan-ss-PEI non-viral vector and investigation of BMP2 signaling regulation

Osteoporotic fractures are still the major health concerns in many developed societies especially when the incidence of that tremendously increased with the aging population. However, the outcomes of osteoporotic fracture treatment have not been entirely satisfactory due to the poor quality of bone substance. Inspiringly, bone morphogenetic protein 2 (BMP2) with the ability to accelerate bone formation showed advantages over the conventional treatment. The only problem needed to overcome is its short half-life which resulted in the requirement of readministration and extremely high cost. As a solution to that, gene therapy provides a promising way to sustainably release this protein at the regeneration site. Since viral vectors have been hampered by genetic toxicity and immunogenicity, nanoscaled non-viral vectors offer an attractive means for gene delivery. Chitosan as non-viral vector has been widely investigated for its excellent biocompatibility. Most efforts have been given to improve its low transfection efficiency. In this study, chitosan was first modified with octaarginine, one of cell membrane penetrating peptides, and showed enhanced transfection activity, but which was not significant as expected. Following that, low molecular weight polyethyleminine (PEI) was introduced to modify chitosan through bioreducible disulfide linkage, denoted as Chitosan-ss-PEI. PEI is an efficient non-viral vector but hampered by molecular-weight dependent toxicity. The developed Chitosan-ss-PEI showed good biocompatibility in MTT assay in three different cell lines, during which cells were maintained 80% of viability when the concentration of this vector was up to 100 μg/mL. The optimal transfection efficiency of Chitosan-ss-PEI was higher than that of PEI 25k and comparable to Lipofectamine in delivering luciferase reporter gene. GFP expression mediated by Chitosan-ss-PEI also showed similar results. Chitosan-ss-PEI was then applied to deliver BMP2 gene to skeletal system cells and exhibited the osteogenic ability. For C2C12 myoblast cells, this system inhibited their myoblast differentiation and induced the osteogenic differentiation. It also showed stronger effect in promoting the differentiation of immature osteblast-like MG63 cells and in inducing C3H10T1/2 mesenchymal stem cells osteogenic differentiation in term of ALP activity and mineralization ability compared with other commercial available non-viral vectors. Primary MSCs such as bone marrow stromal cells (BMSC) and human umbilical cord blood mesenchymal stem cells (hUCB-MSC), are usually more difficult to transfect, but they showed stronger osteogenic differentiation ability induced by this system comparing with the cell lines. BMP2 usually requires extremely high concentration to realize its function. Through the investigation of BMP2 signaling regulation in this study, it was found that parathyroid hormone (PTH) could increase the access of BMP2 ligands to their receptors by negatively influencing BMPs antagonist network, resulted in enhanced BMP2 activity in bone remodeling and in promoting the commitment of MSC to osteoblast lineage both in vitro and in vivo. This course involved the endocytosis of PTHR with a complex of LRP6, which organized antagonist network on the cell surface to shield the BMPs receptors. Novel approaches are expected to be developed based on this mechanism with the purpose of intensifying the therapeutic effect of BMPs.published_or_final_versionOrthopaedics and TraumatologyDoctoralDoctor of Philosoph

Quantitative Analysis of Cited Papers in Research of Soil and Water Conservation from 1994 to 2006

利用中国知识资源总库的中国引文数据库检索《水土保持研究》在1994-2006年所载论文的被引用情况,采用文献计量学方法对该刊1994-2006年载文被《中国期刊文献引证数据库》来源期刊引用的情况进行统计分析及评价。从文献引证的角度透视《水土保持研究》的学术水平。该刊1994-2006年共载文2098篇,被引用2018篇,占载文总数的96.62%。2001-2006年该刊影响因子、即年指标等文献计量指标逐年上升,分析表明:《水土保持研究》载文质量较高,在该学科有较大的学术影响力

一种改进的基于训练序列的OFDM符号同步算法

同步对于OFDM（正交频分复用）系统非常重要。针对S&amp;C算法和Park算法定时模糊的问题,提出一种改进的符号同步算法。该算法先通过接收信号与训练序列进行互相关确定OFDM符号的可能起始位置,然后再滤波进一步确定OFDM符号准确的起始位置。仿真结果表明:改进算法的定时度量在低信噪比和多径衰落信道环境下都具有脉冲型峰值,较传统方法有较小的均方误差

基于PCE的ASON路由技术研究

由因特网工程任务组（IETF）路径计算单元（PCE）工作组定义的基于PCE的多协议标签交换/通用多协议标签交换（MPLS/GMPLS）网络结构能有效解决多层网络中端到端的路径计算问题。文章从自动交换光网络（ASON）路由技术的发展趋势出发,根据有关PCE的RFC文档对PCE的体系结构、发现机制和策略进行了较为系统的说明,重点对PCE在ASON路由中的应用及PCE路径保护和恢复机制进行了阐述,表明PCE技术将成为ASON路由技术的首要选择

基于rbcL-a和ITS的枸杞鉴别、遗传关系分析及ITS假基因的发现

利用rbcL-a及ITS序列作为分子标记,对14个枸杞种或品种及1个伪品白刺进行了真伪鉴别及遗传关系分析。CTAB法提取总DNA,用通用引物对rbcL-a和ITS序列扩增、克隆、测序及分析,T克隆解决部分样品ITS无法成功测序问题。rbcL-a和ITS序列通用引物可在所有样品中成功扩增,rbcL-a序列全长643 bp,在14个枸杞种或品种中完全一致,与白刺具有45bp(7%)的碱基变异。ITS序列在14个枸杞种或品种中长度变异范围为513-692 bp。采用UPGMA法构建系统树,可区分白刺与枸杞,黑果枸杞和宁夏枸杞各品种分别聚为两支,云南枸杞、韩国枸杞及黄果枸杞则聚在宁夏枸杞大分支内。rbcL-a序列可有效进行枸杞真伪鉴别,ITS序列可用于枸杞品种鉴定及遗传关系分析。此外,本研究首次揭示了枸杞品种及个体内的ITS多态性,发现在枸杞中存在ITS假基因这一现象,为未来利用ITS序列进行枸杞系统进化研究提供一些新的参考。</p