Mutagenic EFFects of Nd +3 :YAG Laser on Aspergillus Niger

本文采用nd＋3：yAg激光辐照柠檬酸生产菌黑曲霉孢子，辐照后进行培养和发酵试验、分析测定不同辐照时间黑曲霉孢子的存活率、黑曲霉菌丝体生长繁殖及代谢产酸的速度、主要代谢产物柠檬酸的产量及淀粉糖化酶活力等的变化。实验结果表明，nd＋3：yAg激光辐照后各实验组发生了不同程度的变化，说明该激光能诱发黑曲霉细胞的遗传变异，可用于诱变选育柠檬酸生产的优良高产菌株In this paper, a Nd +3 :YAG laser was used to irradiate spores of the Aspergillus niger, a citric acid-producing strain.The spores irradiated were then cultured and Fermentated.Variations in a survival rates of the spores, rates of growth, propagation, and the metabolic production acid of the hyphae, yield of the main metabolite citric acid and vitality of the saccharidase, etc .were determined and analysed.The experimental results show that the various experimental groups, irradiated by the Nd:YAG laser, had changes in diFFerent degrees.This indicates that the laser can induce Aspergillus niger cells to mutate and may possibly be applied to selectively breed new good quality strains with high yield For production of the citric acid

The eFFects of inFrasound and magnetic Field upon Aspergillus Niger,Enzymes and distilled water

用适当的次声频率和声压来处理黑曲霉孢子（微生物），能使其柠檬酸率增加7%左右，同时用磁场和低频磁场作对比处理，其结果亦同。此外，用此类方法处理五种生物酶和蒸馏水时，其紫外光谱的特征蜂的吸光值均有所增加，与增加酶在缓冲剂中的浓度或水的温度作用相同。而在用此二法处理黑曲菌孢子的发酵液时，发现其主酶（α—淀粉酶）的活力及菌丝体重量也对应地增加，和用同法处理此类纯酶时其活力不能增加的一些报道加以对比。InFrasound and magnetic Field are used to treat Aspergillus Niger.And we Find that the production rate of critic acid, the activity of enzyme and the weight of hypha are all increased at the same rate.Also,these two methods are used to treat some enzymes and distilled water and we get an increase in absorbands of UV spectra of them, similar to the eFFects obtained, by increasing the concentration of onzymes or by increasing the temperature of water