本文采用nd＋3：yAg激光辐照柠檬酸生产菌黑曲霉孢子，辐照后进行培养和发酵试验、分析测定不同辐照时间黑曲霉孢子的存活率、黑曲霉菌丝体生长繁殖及代谢产酸的速度、主要代谢产物柠檬酸的产量及淀粉糖化酶活力等的变化。实验结果表明，nd＋3：yAg激光辐照后各实验组发生了不同程度的变化，说明该激光能诱发黑曲霉细胞的遗传变异，可用于诱变选育柠檬酸生产的优良高产菌株In this paper, a Nd +3 :YAG laser was used to irradiate spores of the Aspergillus niger, a citric acid-producing strain.The spores irradiated were then cultured and Fermentated.Variations in a survival rates of the spores, rates of growth, propagation, and the metabolic production acid of the hyphae, yield of the main metabolite citric acid and vitality of the saccharidase, etc .were determined and analysed.The experimental results show that the various experimental groups, irradiated by the Nd:YAG laser, had changes in diFFerent degrees.This indicates that the laser can induce Aspergillus niger cells to mutate and may possibly be applied to selectively breed new good quality strains with high yield For production of the citric acid
