Maritime Industries in ASEAN-5：Comparative Analysis

东盟五国（印度尼西亚、马来西亚、菲律宾、新加坡和泰国）均为海洋国家，海域辽阔，海洋资源丰富。近年来，东盟五国的海洋经济迅速发展，海洋产业成为国民经济的重要组成部分。 本文借鉴和吸收海洋经济学理论与方法，以东盟五国海洋经济的发展为主线，阐述各国主要海洋产业发展的现状和特征，探讨这些国家主要海洋产业对国民经济的贡献，并对各国海洋经济的发展战略与政策进行分析，最后提出中国-东盟海洋产业合作的对策建议。全文结构如下：第一章，导论部分。阐述选题的背景和意义、国内外文献综述、论文框架和主要内容。第二章，海洋经济及相关理论概述。对海洋经济进行定义和分类，介绍世界各国关于海洋经济产业部门的界定和分类，阐述海...ASEAN countries, especially Indonesia, Malaysia, Philippines, Singapore and Thailand, are ocean countries, possessing vast waters and tremendous marine resources. In recent years, for the rapid development of marine economy, maritime industries had become an indispensable component of the five ASEAN countries’ national economy. Based on the theory and methods of maritime economics, this paper inv...学位：经济学硕士院系专业：南洋研究院_世界经济学号：2552010115208

国有资产首次“晒账单”:公开是最好的监督

背景材料:2018年10月24日,国务院首次向全国人大常委会作《国务院关于2017年度国有资产管理情况的综合报告》,分别对企业国有资产(不含金融企业)、金融企业国有资产、行政事业性国有资产、国有自然资源资产四类国有资产情况进行了报告。这是我国国有资产首次公开亮相"晒家底"

厚颌鲂的年龄结构及生长特性

以鳞片作为鉴定年龄的材料,对龙溪河厚颌鲂(Megalobrama pellegrini)种群开展年龄结构与生长特性的研究。结果表明,厚颌鲂鳞片年轮结构呈疏密切割型,年轮特征显著,可用于年龄鉴定,3~4月是年轮形成高峰时间。种群由0~7龄共8个龄组组成,以低龄个体为主,1~3龄个体占83.14%,体长分布主要集中在130~250 mm间(77.71%)。体质量分布主要集中在50~250 g(64.33%)。群体总性比为♀∶♂=1∶1.03,符合1∶1理论比值。体长和鳞径呈直线关系,体长和体质量呈幂函数关系

广西恭城勉瑶仪式神画研究

勉瑶广泛分布于中国南部以及东南亚北部等国家与地区,因此他们传承的应用于传统宗教仪式中的神画也随着他们的足迹分布于世界各处。通过对广西恭城瑶族自治县勉瑶师公传承的仪式神画种类与内容的个案调查,考察恭城与周边勉瑶地区仪式神画在种类与内容上存在的异同之处。探讨不同地区的勉瑶仪式神画体现出来的勉瑶宗教信仰的普遍性与特殊性的问题。2016年度中国南方与东南亚民族研究中心科研项目“广西过山瑶仪式神画之研究”(2016ZYB001

Studies on the Cloning of Halo-tolerant-related Genes from Eukaryote and Procaryote

根据原核生物基因无内含子的特点，本实验参照大肠杆菌与耐盐性相关的甘露醇-1-磷酸脱氢酶（mtlD）基因的保守序列设计一对引物，以极端嗜盐细菌假单胞杆菌（Pseudomonassp.cn4902）的总DNA为模板，通过PCR扩增出mtlD结构基因，将该基因克隆至pMD18-T载体，测序结果表明：假单胞杆菌的mtlD结构基因长1149bp，编码383个氨基酸。本序列与多种生物的mtlD基因高度同源，与E.coliK12的mtlD基因的同源性高达99%。将该结构基因与pBV220质粒构建成大肠杆菌高效表达重组载体pBH。SDS-PAGE电泳表明：含pBH的E.coliJM101转化子产生特异的甘露醇...According to the essential character that no intron can be found in the genes of prokaryote, a pair of primer has been designed on the basis of the conserved sequence of halo-tolerant-related mtlD (mannitol-1-phosphate dehydogenase) gene in Escherichia coli. The mtlD gene of halophilous Pseudomonas sp.cn 4902 has been copied by the PCR reaction of making use of its genomic DNA as template and inse...学位：理学硕士院系专业：生命科学学院生物学系_生物化学与分子生物学学号：19992600

Cloning of Halo-tolerant-Related Gene(s) from Halobacterium halobium

将盐生盐杆菌总 DNA的 Bam HI不完全酶切片段与质粒 p U C19连接成重组质粒 ,并以重组质粒转化 E. coli JM10 1.经 X- Gal- IPTG法筛选白色重组子 ,结合高盐平板检测 ,已经获得 3株比原受体菌的耐盐性提高 30 %～ 67%的转化子 .重组质粒酶切电泳分析表明 ,被克隆的盐生盐杆菌含耐盐相关基因的 DNA片段长度在 1.0～ 3.5kb之间 .Three recombinant plasmids which have been reconstructed with plasmid pUC19 and random DNA fragments of Halobacterium halobium were transferred to E. coli JM101. As the result, the tolerant level against to NaCl of these bacteria transferred has been raised from 0.9 to 1.2~1.5 mol·L -1 respectively. The molecular weight of these DNA fragments inserted are 1.0 , 1.8 and 3.5 kb according to the evidents of agarose eletrophoresis. So it could be referred that these three DNA fragements may contain halo tolerant related gene(s).福建省自然科学基金!资助项目 ( C960 0 4

八角格子光子晶体光纤模式和色散特性研究

文章基于带有各向异性完全匹配层（PML）吸收边界条件的紧凑二维频域有限差分法对八角格子光子晶体光纤（O-PCF）的模式和色散特性进行了研究。利用有效面积法分析了八角格子和六角格子光子晶体光纤（H-PCF）的基模和多模截止特性,得到非限制模、基模及多模的相图。比较发现,填充率和空气孔间距相同时,O-PCF的单模运转区域宽于H-PCF,更易用于色散补偿

我国财政转移支付制度探析

财政转移支付制度是缩小区域差距、城乡差距,实现社会稳定和经济可持续发展的重要抓手之一。目前我国的财政转移支付制度尚未充分发挥应有的作用。本文从制度视角切入对我国财政转移支付问题的研究,分别针对制度设计、制度实施和制度监督方面存在的问题,提出建设和完善我国财政转移支付制度的政策建议,以推动实现基本公共服务全面均等化

CLONING AND EXPRESSION OF MANNITOL-1-PHOSPATE DEHYDROGENASE GENE OF PSEUDOMONAS sp. cn 4902

参照几种生物的甘露醇 1 磷酸脱氢酶基因 (mtlD)的序列设计引物 ,以极端耐盐的假单胞菌 (Pseudomonassp cn 4 90 2 )的总DNA为模板 ,采用PCR扩增、构建重组高效表达载体以及生物信息学研究等方法 ,进行基因克隆、表达及功能定性等研究。结果表明 ,克隆获得一长为 114 9bp的基因。经蛋白质保守区域研究 ,初步判别该基因为mtlD结构基因。将该基因与pBV2 2 0质粒构建成高效表达原核重组载体pBH。SDS PAGE电泳表明 ,含pBH的转化子产生特异的、分子量约为 4 1kD的蛋白带 ,表达量约占菌体可溶性蛋白 6 7%。转化子的耐盐水平比对照提高了约 1/5。在含 0 9mol/LNaCl的液体培养基中 ,转化子培养 2 4h后其生物量约是对照的 10 2倍 ,甘露醇含量约是对照的 4 1倍。可见 ,假单胞菌的甘露醇 1 磷酸脱氢酶基因是一个重要的耐盐相关基因 ,该基因已在GenBank登记 ,代号为AY112 6 96。According to the essential characteristic of the genome of prokaryotes that no intron can be found in the genes,an attempt was made to find a new gene of mannitol-1-phosphate dehydrogenase ( mtl D) in Pseudomonas sp. cn 4902 by means of PCR amplification. Based on the short conserved sequence of the halo-tolerant-related gene in some microorganisms,a pair of primers were designed for PCR amplification with the template of genomic DNA of Pseudomonas sp. cn 4902. An approximate 1. 1kb DNA fragment of PCR amplification product could be found in the agarose gel electropherogram. The fragment was,then,retrieved,a poly A “tail” added and finally lined together with plasmid pMD18-T overnight to form the reconstructed vectors. The white colonies were picked out,some of them were cultivated and the plasmids were extracted in the transformants and digested by two endonucleases Bam HⅠ and Sal Ⅰ for the detection of the reconstructed vectors after agarose gel electrophoresis. The inserted DNA fragment was sequenced and transferred to the multicloned site of high efficiency expression plasmid pBV220 in E.coli JM101 to constitute a new plasmid named pBH. The tolerance levels against to serious concentrations of NaCl and its protein expression products were researched. It was clear that the cloned gene was 1149bp in length and coded for 383 amino acid residuals. Using the biological informatics in GenBank we found that the nucleotide sequence and deduced amino acid residuals of this gene share more than 90% homology with the mtl D gene of other organisms,including E. coli K12. The result of protein SDS-PAGE electrophoresis of transformants indicated that a 41kD protein coincided with the molecular weight of the transferred mtl D gene,which was 6.7% of the total protein,was produced in this transformants under the condition of 42℃ hot shock. As a result,the highest tolerant level against NaCl of the transformants cultivated in the agar LB medium was raised from 0.9 to 1.1mol/L,which was 22% higher than that of the control. Meanwhile,the biomass of transformants cultivated in the liquid medium containing 0.9mol/L NaCl was about ten-fold that of the control. It was believed that the cloned gene should be a mannitol-1-phosphate dehydrogenase ( mtl D) gene,one of the key genes responsible for osmoregulation in the cells of organisms. The successful cloning and expression of the mtl D gene supplied a good foundation for cultivation of halo-tolerant-related crop plants in the future.科技部转基因植物专项基金资助 ,J0 0 B 0 14号 ;; 厦门市科委科技发展基金资助项目 ,35 0 2 2 2 0 0 0 10 4