Expression of recombinant hARRG cDNA in E.coli and purification of hARRG protein

目的　构建含重组人类抗砷相关基因 (humanarsenicresistencerelatedgene ,hARRG)的表达载体 ,诱导其在转化菌表达 ,分离纯化表达蛋白 ,研究该蛋白质的理化性质、抗砷功能和免疫活性 ,深入研究人类对砷化物的抵抗作用。方法　将hARRGcDNA开放阅读框亚克隆到原核表达载体Pet11C中 ,用异丙基 - β -D -硫代半乳糖苷 (IPTG)诱导表达蛋白质 ,利用阴离子交换柱Sepharose纯化蛋白质 ,SDS -PAGE胶电泳观察结果。 结果　将hARRGcDNA成功亚克隆到原核表达载体Pet11C中 ,并成功在大肠杆菌中表达 ,表达的hARRG蛋白占菌体蛋白的 5 %左右 ,该蛋白质被分离纯化。结论　原核表达载体Pet11C可以在大肠杆菌中表达hARRGcDNA ,可用阴离子交换柱Sepharose纯化抗砷相关蛋白质Objective To construct expression vector of the recombinant human arsenic resistance related gene (hARRG),induce its expression in DE\-3 and isolate and purify expression product,for studying the physiochemistry characteristic,function and immune activity of the protein,and further researching the arsenic resistant effects of human.Methods hARRG cDNA was subcloned into prokaryotic expression vector Pet11C.The recombinant protein expression was induced by IPTG,then,the protein was purified by anions Ion-exchange column Sepharose and examined by SDS-PAGE gel.Results hARRG cDNA was successfully subcloned into prokaryotic expression vector Pet11C and expressed in E.coli and the protein was purified by anions Ion-exchange column successfully.Conclusion Pet11C excpression vector containing hARRG cDNA wassuccessfully constructed,the cell DE\-3 transformed with expression vector capable of expression the gene and a hARRG protein could be purified by anions Ion-exchange column Sepharose.国家自然科学基金资助项目 (30 0 60 0 74

Biodiversity of the ectomycorrhiza on the rare and endangered tree species Tuga chinensis tchekiangensis(Flous) Cheng in Wuyishan Nature Reserve

对武夷山国家级自然保护区残遗与濒危树种南方铁杉植物群落历经了6年的调查,共采集了123种外生菌根,经显微与超显微技术鉴定了84种,这一结果是迄今为止已发表的,在同一树种中菌根类型最多的记录.它不仅证明了武夷山地区的菌物区系由于处于东西相连,南北贯通的位置上,而且从区系成分上据有温热两带俱全的特点,因而形成生物多样性,并从共生菌的角度再度揭示了物种协同进化的规律.Systematic investigation was made on ectomycorrhizae in the woods of a variety of Tsuga chinensis,tchekiangensis(Flous) Cheng,in Wuyishan Nature Reserve in the past six years.During that period,120 ectomycorrhiza samples were collected and 84 types identified by analysing their macro-micro-and ultrastructure.This is the first time that so many types of mycorrhiza in the forests of the species are reported on.This result has proved Wuyishan's geographical position at the cross where a fungi growing area links the east and west,south and north.Thus the region owns the characteristics of both the temperate and the subtropical zone and it is thus such peculiarity whereby its biodiversity is created.Moreover,considering the results of this investigation in the regard of symbiosis between plants and fungi,their co-evolution is ascertained once more.国家自然科学基金资助项目(39770004);; 福建省自然科学基金资助项目(C97003,D0410002

Two I-h-symmetry-breaking C-60 isomers stabilized by chlorination

通讯作者地址: Xie, SY (通讯作者), Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 地址: 1. Xiamen Univ, State Key Lab Phys Chem Solid Surfaces, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 2. Xiamen Univ, Dept Chem, Coll Chem & Chem Engn, Xiamen 361005, Peoples R China 电子邮件地址: [email protected] abiding surprise in fullerene science is that I-h-symmetric buckminsterfullerene C-60 (ref. 1) (I-h-C-60 or C-#1,812(60), the nomenclature specified by symmetry or by Fowler's spiral algorithm(2)) remains the sole C-60 species experimentally available. Setting it apart from the other 1,811 topological isomers (isobuckminsterfullerenes) is its exclusive conformity with the isolated-pentagon rule(3), which states that stable fullerenes have isolated pentagons. Although gas-phase existence of isobuckminsterfullerenes has long been suspected(4-7), synthetic efforts have yet to yield successful results. Here, we report the realization of two isobuckminsterfullerenes by means of chlorination of the respective C-2v-and C-s-symmetric C-60 cages. These chlorinated species, (C60Cl8)-C-#1,809(1) and (C60Cl12)-C-#1,804(2), were isolated in experimentally useful yields. Structural characterization by crystallography unambiguously established the unique pentagon-pentagon ring fusions. These distinct structural features are directly responsible for the regioselectivity observed in subsequent substitution of chlorines, and also render these unprecedented derivatives of C60 isomers important for resolving the long-standing puzzle of fullerene formation by the Stone-Wales transformation scheme(8-11).NNSF of China,20525103 ,20531050 ,20721001 , 20571062 ,20425312 973 Program 2007CB81530

对中国移动、中国联通价格竞争的一种解释——存在转换成本的双寡头价格博弈

本文建立了一个存在转换成本的双寡头价格博弈模型,用以解释我国移动通信市场中中国移动和中国联通的价格竞争行为。研究认为,在用户数量增长的市场中,初始市场份额小的企业将制定相对于初始市场份额大的企业更低的价格,博弈的结果会导致初始市场份额大的企业的市场份额下降

基于RFID的生产线物料监控系统的设计与应用

介绍了近年兴起的射频识别(RFID)技术的特点,并给出了一个实际加工生产线RFID的应用案例;说明了生产线物料监控系统的总体软、硬件架构体系;最后介绍了RFID智能操作终端

允饲植物源复方制剂对草鱼(Ctenopharyngodon idellus)组织形态和生长性能的作用研究

为了进一步评估由白芍(Radix paeoniae Alba)、杜仲(Eucommia ulmoides)和知母(Rhizoma anemarrhenae)组成且具有免疫增强作用的复方制剂A、B、C对草鱼(Ctenopharyngodon idellus)组织形态和生长性能方面可能存在的风险,本试验将二龄草鱼随机分成12个试验组和2个对照组,分别连续投喂含有不同复方制剂的饲料和空白饲料,在试验第7、14、21、28天分别随机从对应试验组中采集5尾草鱼肝胰脏、脾脏、肾脏和肠道进行切片制作和组织形态分析;在试验第0、29天分别随机从对应试验组中采集10尾草鱼进行肥满度、增重率、特定生长率、脏体比和肠道承重力测定。结果表明,分别以2%、4%剂量添加于草鱼饲料中的复方制剂A、B、C对试验草鱼阶段增重率、特定生长率、饵料系数、肝胰体比、肾体比和肠道承重力系列指标均无异常影响。组织形态分析方面,复方制剂A对草鱼肝胰脏、脾脏、肾脏和肠道具有良好的安全性,而复方制剂B、C经连续投喂21天起,对应剂量组草鱼肝胰脏、肾脏逐渐呈现不同程度的组织病变。本文得出结论认为,即使完全符合允饲要求的天然植物品种,缺乏基于较全面的评价维度试验证明其具有使用安全性,则容易仅关注允饲天然植物在效果表达的表象,而忽略其诱发的组织病变造成组织器官的功能衰退

允饲植物源复方制剂对草鱼(Ctenopharyngodon idellus)的免疫增强作用研究

为筛选适合添加于草鱼(Ctenopharyngodon idellus)饲料的植物源免疫增强剂,本试验以白芍(Radix paeoniae Alba)、杜仲(Eucommia ulmoides)和知母(Rhizoma anemarrhenae)三种允饲植物配制的复方制剂A、B和C为研究对象,开展其对草鱼非特异性免疫功能的比较研究。试验将二龄草鱼随机分成6个试验组和1个对照组,试验组和对照组按鱼体重量的3%每天分别投喂添加复方制剂的饲料和空白饲料。试验第7、14、21、28天分别采集试验草鱼血液,用于检测血液细胞组分、血清溶菌酶、血清SOD;试验第29天,对各组试验草鱼进行腹腔注射嗜水气单胞菌活菌,记录攻毒后14天内各组草鱼的存活率。结果表明,与对照组相比,试验第14天时,以2%和4%剂量添加的复方制剂A分别显著提高草鱼白细胞吞噬活性128%和143%,分别提高草鱼血清溶菌酶活性69%和85%,分别提高草鱼血清SOD活性61%和55%;连用28天对草鱼的保护率分别达到49%和55%,复方制剂A对草鱼非特异性免疫增强效果优于复方制剂B和C

允饲植物源复方制剂对草鱼(Ctenopharyngodon idellus)的免疫增强作用研究

为筛选适合添加于草鱼(Ctenopharyngodon idellus)饲料的植物源免疫增强剂,本试验以白芍(Radix paeoniae Alba)、杜仲(Eucommia ulmoides)和知母(Rhizoma anemarrhenae)三种允饲植物配制的复方制剂A、B和C为研究对象,开展其对草鱼非特异性免疫功能的比较研究。试验将二龄草鱼随机分成6个试验组和1个对照组,试验组和对照组按鱼体重量的3%每天分别投喂添加复方制剂的饲料和空白饲料。试验第7、14、21、28天分别采集试验草鱼血液,用于检测血液细胞组分、血清溶菌酶、血清SOD;试验第29天,对各组试验草鱼进行腹腔注射嗜水气单胞菌活菌,记录攻毒后14天内各组草鱼的存活率。结果表明,与对照组相比,试验第14天时,以2%和4%剂量添加的复方制剂A分别显著提高草鱼白细胞吞噬活性128%和143%,分别提高草鱼血清溶菌酶活性69%和85%,分别提高草鱼血清SOD活性61%和55%;连用28天对草鱼的保护率分别达到49%和55%,复方制剂A对草鱼非特异性免疫增强效果优于复方制剂B和C

不同厚度PTFE增强复合膜的SPE水电解性能

采用溶液浇铸法制备Nafion／PTFE复合膜，测试了复合膜的含水率、尺寸稳定性、机械强度和质子电导率，并将其应用到固体聚合物电解质（SPE）水电解中。在制备复合膜的同时将催化层喷涂到膜的两侧，最后复合膜与催化层共同结晶，使其一体化，增强复合膜与催化层的结合强度，满足水电解领域应用要求。评价不同厚度Nafion／PTFE复合膜制备的CCM对水电解池性能的影响并与Nafion112膜比较。相同操作条件下厚度小于30μm的复合膜水电解槽电压低于Nafion112．降低水电解能耗和膜成本