逆转素对人肝癌细胞HepG2增殖、克隆形成及凋亡的影响

目的探讨逆转素（reversine）对人肝癌细胞HepG2细胞增殖、克隆形成和凋亡的影响。方法人肝癌细胞HepG2经不同浓度reversine(0.1～1.6μmol·L-1)处理后,应用MTS法检测其对细胞增殖能力的影响;克隆形成实验评估reversine作用于肝癌细胞HepG2的远期效应;流式细胞术Annexin V-FITC/PI双染法检测reversine处理后肝癌细胞HepG2的凋亡率;Western blot法检测凋亡相关蛋白PARP、Bax、Bcl-2及Bcl-xL表达水平的变化。结果与对照组（DMSO）相比,不同浓度reversine对人肝癌细胞HepG2的增殖均有抑制作用（P<0.05）,且呈剂量依赖关系,IC50为0.94μmol·L-1。Reversine可以明显抑制Hep G2细胞的克隆形成能力（P <0. 05）,且较高浓度reversine处理后,Hep G2细胞几乎不能形成肉眼可见的细胞克隆; reversine可以诱导Hep G2细胞凋亡（P <0. 05）,且细胞的凋亡率与reversine的浓度呈正相关; Western blot结果显示,reversine孵育48 h后,Hep G2细胞中活化PARP、Bax的表达明显上调;同时,Bcl-2及Bcl-xL蛋白的表达下降。结论 Reversine可以明显抑制人肝癌细胞Hep G2细胞的增殖和克隆形成能力,并且可诱导Hep G2细胞的凋亡,其诱导凋亡与线粒体凋亡途径相关。福州总医院创新团队项目(No 2014cxtd05

Distributions of dissolved organochlorine pesticides and polychlorinated biphenyls in China coastal waters

分析调查了中国部分海域海水中溶解态有机氯农药(OCPS,25种)和多氯联苯(PCbS,6种)的残留分布特征。结果表明,所有采样点都不同程度地受到了OCPS和PCbS的污染,其中福建省福州平潭和莆田湄洲岛的OCPS污染较为严重,浓度分别达到1752.59和796.19 ng/l;而辽宁省的大连老虎滩OCPS污染较轻,浓度为53.63 ng/l。各采样点海域海水中HCHS污染程度一般高于ddTS,HCHS主要以早期残留为主;根据ddT/ddTS的比值推测,在福州平潭、莆田湄洲岛和珠海淇澳岛水体显示有新的ddT输入。在各海域水体中溶解态PCbS的浓度为33.55--474.92 ng/l,均超过了美国环保局规定的海水浓度限值。因此目前我国沿海海域水体中持久性有机污染物有机氯农药和多氯联苯污染仍不能忽视。The concentrations of organochlorine pesticides(OCPs,25) and polychlorinated biphenyls(PCBs,6) in seawater collected from China coast were investigated.The results showed that concentrations of OCPs ranged from 53.63 to 1752.59 ng/L in seawater,and the highest value was in Pingtan(Fuzhou) while the lowest value was in Laohutan(Dalian,Liaoling province).At all of sampling sites,the concentrations of DDTs and HCHs were lower than the limits of national seawater quality standards(class 1),and the concentration of HCHs was higher than that of DDTs.The ratios of DDT/DDTs showed new DDT into Pingtan(Fuzhou),Meizhoudao(Putian) and Qi'aodao(Zhuhai,Guangdong Province),whereas the isomers characteristics for HCHs showed the residues at all sampling sites.The concentrations of dissolved PCBs ranged from 33.55 to 474.92 ng/L in seawater,which were higher than the limit of EPA seawater quality standard.However,the occurrence of OCPs and PCBs in seawater along China coast should be concerned.国家自然科学基金(40476048;20777060);厦门大学新世纪优秀人才计划基

Establishment of a method to quantitatively detect FLT3 internal tandem duplication in acute myeloid leukemia with denaturing high-performance liquid chromatography

目的:建立一种应用变性高效液相色谱技术(dHPlC)相对定量检测急性髓细胞白血病(AMl)患者fMS样酪氨酸激酶3(flT3)基因的内部串联重复(ITd)突变的方法。方法:根据flT3-ITd突变基因多位于14外显子而设计引物,用聚合酶链反应(PCr)方法特异性扩增121例AMl患者flT3-ITd突变基因,再用dH-PlC技术相对定量检测flT3-ITd等位基因突变的情况;与毛细管电泳法(CE)检测突变的结果对比进行该方法的有效性检验;最后与121例样品PCr扩增产物的测序结果进行对比。结果:经dHPlC分析后均能得到特征性的洗脱峰。121例样本中检测到flT3-ITd突变阳性的样本13例,总阳性率为10.7%,阳性突变等位基因的比例不一,分布范围中位数为34.5%(11.4%-80.2%),为21-87 bP单个插入片段。阳性率和突变比例与CE方法检测结果相比较均无显著差异(P>0.05),并与121例样本flT3-ITd扩增PCr产物基因测序结果一致。结论:成功建立了一种应用dHPlC相对定量检测AMl患者flT3-ITd基因突变的方法。AIM: To establish a relatively-quantitative method to detect the internal tandem duplication(ITD) mutation of Fms-like tyrosine kinase 3(FLT3)gene in acute myeloid leukemia(AML) patients using denaturing high-performance liquid chromatography(DHPLC).METHODS: According to the fact that much more FLT3-ITD mutations are located in exon 14,we designed the primers,and use the method of polymerase chain reaction(PCR) to specifically amplify FLT3-ITD mutation gene in 121 cases of AML,and relatively quantified the situation of mutant allelic gene of FLT3-ITD by the method of DHPLC.The effectiveness of DHPLC was verified by the method of capillary electrophoresis(CE).The sequenced results from PCR amplified products of 121 samples were compared.RESULTS: A characteristic of elution peak was detected by DHPLC with 10.7% overall positive rate(13/121) and varied in the proportion of mutant alleles,with a single duplicated insert fragment from 21 bp to 87 bp.The median range of mutant alleles was 34.5%(11.4%-80.2%).No significant difference of the positive rates and mutation proportions between the results with DHPLC and the results with CE method was observed.The results of FLT3-ITD mutant gene of 121 samples were consistent with the results using sequencing method.CONCLUSION: A relatively-quantitative method to analyze AML patients with FLT3-ITD mutation by DHPLC is successfully established

Imposex of Thais clavigera and Cantharus cecillei in the coastal waters of Xiamen

海产腹足类性畸变以其对有机锡的敏感性、特异性和不可逆性而成为海洋有机锡污染的理想指示种。调查研究了厦门海域岩相海岸带疣荔枝螺(THAIS ClAVIgErA)和甲虫螺(CAnTHAruS CECIllEI)的性畸变情况,通过生物学指标IOI(性畸变率)、rPSI(相对阴茎发展指数)、VdSI(输精管发展指数)及SrI(性比指数)综合评价其性畸变发展程度,间接指示厦门周边海域的有机锡污染程度。研究结果发现,在采集疣荔枝螺的17个站点中有5个站点种群性畸变率为100%,其中宝珠屿种群性畸变程度最为严重,VdSI达4,采集甲虫螺的4个站点的种群性畸变率均为100%。性畸变程度较严重的种群普遍集中于厦门西港,并且表现出由港内到港外逐渐减轻的趋势,而白城、会展中心和大嶝岛等位于开阔的厦门东海域的种群性畸变程度最轻,与往年厦门西海域有机锡污染监测数据相比一致,体现出性畸变程度与有机锡污染程度及海港码头远近之间的相互关系。The degree of imposex,i.e.the imposition of male characteristics onto females,in neogastropods can be used as a sensitive and accurate biomarker to evaluate organotin contamination in coastal marine environment.Now,the imposex status in females of Thais clavigera and Cantharus cecillei in 17 sites was investigated to evaluate the organotin contamination along Xiamen Bay during 2006 and 2007.The four indices including incidence of imposex(IOI),relative penis size index(RPSI),vas deferens sequences index(VDSI) and sex ratio index(SRI) were used comprehensively to assess the imposex status in order to ascertain the extent of organotin bioavailability in Xiamen Bay.Based on this research,T.clavigera in 5 sites and C.cecillei in all survey sites exhibited the IOI of 100%.At Bao ZhuYu Islet,the imposex level was the most serious with maximum VDSI value of 4.In general,the imposex level decreased from the inner to the outer of Xiamen Western Harbour,and the populations in the open Xiamen Eastern Waters including Bai Cheng,Convention and Exhibiton Center and Dadeng Island showed a lower imposex level.These results were accordant with the concentrations of organotins in 2005.The imposex degree also increased with increasing organotin contamination and decreasing distance from shipping facilities.国家自然科学基金资助项目(40476048);厦门大学近海海洋环境科学国家重点实验室开放基金资助项目;厦门大学新世纪优秀人才计划基金资助项

武汉东湖不同湖区底泥总磷含量及变化的研究

以武汉东湖为对象 ,研究了 1 998.0 3&mdash; 1 999.0 2期间不同营养水平湖区底泥中 (0&mdash;5cm ,5&mdash; 1 0cm)总磷的含量及季节动态。 6个站平均总磷含量为 1 .1 5mg/g ,同 80年代初相比 ,Ⅰ、Ⅱ站底泥中总磷含量分别增高 1 .42倍和 1 .0 3倍。受污水排放影响较重的 0站磷含量高达 2 .78mg/g,而受污水排放影响较小的Ⅳ、Ⅴ站仅分别为 0 .52mg/g和 0 .50mg/g。东湖底泥中磷年平均含量与湖水中磷年平均浓度相关系数极高 (r=0 .997,n =

武汉东湖不同湖区底泥总磷含量及变化的研究

以武汉东湖为对象，研究了1998.03－1999.02期间不同营养水平营养水平湖区底泥中（0－5cm,5-10cm）总磷的含量及季节动态。6个站平均总磷含量为1.15mg/g，同80年代初相比，Ⅰ、Ⅱ站底泥中总磷含量分别增高1.42倍和1.03倍。受污水排放影响较重的0站磷含量高达2.78mg/g，而受污水排放影响较小的Ⅳ、Ⅴ站仅分别为0.52mg/g和0.50mg/g。东湖底泥中磷年平均含量与湖水中磷年平均浓度相关系数极高（r=0.997,n=5,p<0.02）。通过对不同湖泊的底泥磷含量、水柱磷含量和外源负荷的比较和相关分析可以看出，水柱中磷含量较高，磷负荷较大的湖泊底泥中磷含量也高，可以预测，即使东湖的外源负荷得以控制，巨大的底泥磷内源负荷将会继续对东湖水质构成威胁

Experimental Research on Preparing Potassium Sulfate from Phosphogypsum

以磷石膏、碳酸氢钠、氯化钾为原料,通过二步法制备硫酸钾,主要考察了原料配比、反应时间、反应温度等因素对原料转化率的影响,并采用正交实验法确定最佳工艺条件,结果表明,该方法制备的K2SO4达到了ZBG21006-89一级品标准,本研究具有较好的社会效益、经济效益和环境效益,是一条可行的磷石膏综合利用途径.广西民族学院青年科基金(99HXX00007

Effect of component of bi-functional catalysts on one-step synthesis of dimethyl ethe

采用干磨法,选用不同种类甲醇合成组分和不同种类的甲醇脱水组分制备双功能催化剂,并在高压固定床反应器评价其催化性能。研究表明,催化剂JC207是一种较优的甲醇合成催化剂,其最佳配比为80%。分子筛是一种较好的甲醇脱水催化剂,其最佳配比为20%。通过寿命试验和催化剂的XRD表征发现催化剂晶相没有改变,催化剂结构保持稳定。Bi-functional catalysts were prepared by dry grinding of different components of catalysts for methanol synthesis and methanol dehydration.Their catalytic activity were evaluated with a high-pressure fixed-bed reactor.Results indicated that JC207 was a good catalyst for synthesis of methanol.Its optimum ratio was 20%in bi-functional catalyst.Zeolite was a good catalyst for methanol dehydration.Its optimum ratio was 80%in bi-functional catalyst.Stability test and XRD analysis of bi-functional catalyst confirmed that the crystalline phase of catalyst did not change,and the structure of catalyst remained stable.广西科学研究与技术开发计划资助项目(桂科能0992928-1

Progress of Biomass-syngas Production and Synthetic Liquid Fuels from Biomass-syngas

主要介绍了国内外生物质气化制备合成气工艺以及国内外生物质合成气合成液体燃料的工艺技术。生物质直接气化技术在早期得到了较好的发展,但生物质收集困难,限制了其发展。近几年国内外开发了生物质两段气化工艺技术。该技术通过快速裂解把生物质裂解为能量密度相对较高的快速裂解油(生物油),通过收集生物油制备合成气路线可以降低整个系统的成本,该技术可能成为未来生物质合成液体燃料的工业化技术。The technology of biomass-syngas production from biomass and the technology of synthetic liquid fuels from biomass-syngas at home and abroad were introduced.Biomass gasification technology was developed in early research,but biomass was difficult to collect,so the development of biomass gasification technology was restricted.In recent years,the two-step biomass gasification technology was developed both at home and abroad.This technology is used to transform biomass into fast pyrolysis oil（bio-oil） with high energy density.The bio-oil collected can be used to produce biomass-syngas.The technology can reduce the cost of the entire system,and it will be the industrial technology of synthetic liquid fuel from biomass in the future.广西科学研究与技术开发计划项目(桂科能0992928-1

Cultivation and Characters of the Aerobic Granules for Processing Waste Water in SBR System

好氧颗粒污泥是目前水污染处理领域研究的一个热点.该文利用自制的SBR反应器培养好氧颗粒污泥,对好氧污泥颗粒化的过程及其性能进行研究和表征.用粗盐改变废水的表面张力,用活性炭改变废水的固含率,在人工模拟废水和餐饮废水体系中比较好氧颗粒污泥和絮状污泥对废水COD的去除率.实验结果表明:成熟好氧颗粒污泥性能良好,沉降速度快,对污染物的去除能力较强,其各项理化指标均优于普通活性污泥.在相同的实验条件下,好氧颗粒污泥COD去除率高于传统的絮状污泥.The study of aerobic granular sludge is one of the most popular research areas in the waste water processing.In this paper,self-made SBR reactor was used to cultivate aerobic granular sludge,and the process of aerobic sludge granulation and its performance were studied and characterized.In order to improve the oxygen transfer rate,oxygen consumption rates under different conditions were measured as following： coarse salt was used to change the surface tension of the waste water;activated carbon was used to change the solid holdup of the waste water.Finally,the aerobic granular sludge and the raw sludge removal rate of COD in the waste water were compared in both artificial wastewater system and food wastewater system.The results show that： the performance of the mature aerobic granular sludge is good,the settling rate is quick,the pollutant removal ability is strong,and its physical and chemical indicators are better than the conventional activated sludge.Under the same experimental conditions,4-hour aeration wastewater COD removal rate of aerobic granular sludge is better than conventional flocculent activated sludge.广西青年科学基金资助项目(桂科青0542019);广西科学研究与技术开发计划课题(桂科能0992928-13