对一例脓毒血症伴慢性肾衰竭急性期病人的药学服务

脓毒血症是因感染导致的一种重度炎症性反应综合征（severe inflammatory response syndrome,SIRS）,目前普遍认为脓毒血症并发急性肾衰是因SIRS发生阶段发生氮质产物潴留、代谢性酸中毒,且在病情进展成严重脓毒血症时发生内环境紊乱而致[1]。临床药学服务的要点在于协助医师尽早控制病人的全身感染,保护肾功

Study on Raman Spectra of Several Conformations of Thiophenol on Gold

用Gaussian 98程序在B3LYP/6-311+G**(C,H,S)水平上计算得到苯硫酚(TP)的一套校正因子,对苯硫酚各频率进行了势能分布(PEDs)分析,并对各振动模式进行了详细的指认.同时,在B3LYP/6-311+G**(C,H,S)/LANL2DZ(Au)水平上优化得到苯硫酚的各种形态与金结合,即C6H5SH-Au,C6H5SAu,C6H5S--Au的平衡构型,且在此基础上得到了C6H5SH-Au,C6H5SAu,C6H5S--Au三种形态的计算拉曼谱图.其中苯硫酚金盐(C6H5SAu)的拉曼谱图与实验得到的苯硫酚在金溶胶中的谱图是一致的,由此很好地证明了TP与金形成苯硫酚金盐.The optimized geometries of the clusters C6H5SH, C6H5SAu, C6H5S--Au and C6H5SHAu were calculated using the B3LYP density functional method with the 6-311+G** basis set for C, S, H and LANL2DZ for gold. All the calculations were carried out with the Gaussian 98 program package. Through PEDs (Potential Energy Distributions) analysis, comprehensive frequency assignments of thiophenol were performed and the scaling factors of the thiophenol were found. The calculated Raman spectra of several conformations of the thiophenol adsorbed on gold were obtained. The calculated Raman spectrum of C6H5SAu is in good agreement with the experimental one. Therefore, C6H5SAu is the conformation which is closest to the reality.国家自然科学基金(Nos.20273045,20373046);; 厦门大学固体表面物理化学国家重点实验室开放课题资助项目

杆状病毒用于哺乳动物细胞快速高效表达外源基因的研究

现已发现杆状病毒可进入某些培养的哺乳动物细胞 ,这提示可将杆状病毒作为一种对哺乳动物细胞的新型基因转移载体。对杆状病毒转移载体的改造及对哺乳动物细胞的基因转移方式进行了进一步的研究。以绿色荧光蛋白基因为报告基因 ,利用Bac to Bac系统构建了分别含有正向和反向CMV启动子表达盒的两种重组杆状病毒。可观察到CMV启动子在Sf9细胞中可启动报告基因的表达 ,但表达效率较低。用重组杆状病毒感染后Sf9细胞的培养上清直接与HepG2细胞作用 ,以流式细胞术检测基因转移效率及荧光表达强度 ,发现这两种病毒在相同的感染复数下对HepG2细胞具有相似的基因转移及表达效率。同时 ,利用流式细胞术进一步研究了直接使用重组杆状病毒感染 4d后Sf9细胞的培养上清对哺乳动物细胞进行基因转移的方法。通过对HepG2细胞的实验结果显示 ,将带毒Sf9细胞培养上清 (1.2× 10 7PFU mL)用哺乳动物细胞培养基 1倍稀释后 ,37℃下孵育靶细胞 12h(moi=5 0 ) ,可达到较高的基因转移及表达效率 ,同时不会对细胞造成明显损伤。将重组杆状病毒与脂质体和逆转录病毒这两种系统对HepG2及CV1细胞的基因转移效率进行了比较 ,结果发现在同样未经浓缩等特殊处理的条件下重组杆状病毒对这两种细胞的基因转移效率是最高的。因此可以认为

Study on Raman spectra of several conformations of thiophenol on gold

The optimized geometries of the clusters C6H5SH, C6H5SAu, C6H5S- -Au and C6H5SHAu were calculated using the B3LYP density functional method with the 6-311+G** basis set for C, S, H and LANL2DZ for gold. All the calculations were carried out with the Gaussian 98 program package. Through PEDs (Potential Energy Distributions) analysis, comprehensive frequency assignments of thiophenol were performed and the scaling factors of the thiophenol were found. The calculated Raman spectra of several conformations of the thiophenol adsorbed on gold were obtained. The calculated Raman spectrum of C6H5SAu is in good agreement with the experimental one. Therefore, C6H5SAu is the conformation which is closest to the reality

Zooplanktonic diversity in the western Pacific

西太平洋区是全球海洋生物种源中心,许多类群的最高物种多样性都出现于该区域,因此,在该区开展种类多样性的研究不仅重要和必要,而且具有在跨国尺度上进行综合管理和相互合作的迫切性。本文在西太平洋的浮游动物样品鉴定分类、编目、文献资料整理和分析的基础上,记录和编入西太平洋10个浮游动物类群2,658种(含亚种),隶属于206科675属,其中水螅水母类99科251属697种,栉水母类12科22属59种,浮游软体动物14科35属86种,介形类8科89属416种,桡足类51科156属908种,糠虾类4科58属202种,磷虾类2科8属56种,十足类8科22属105种,毛颚类5科8属48种,被囊类5科26属81种。The western Pacific region has been operating as a centre for the origin of marine biodiversity: the richest diversity of many marine taxa was found in these waters.Therefore,biodiversity research and con-servation efforts in this area are necessary in order to promote the integrative and international management of this resource.The present work is a compilation of numbers of all the families,genera and species of ma-jor taxa of zooplankton known in the western Pacific Ocean(106°–150°E,0°–44°N).In all,2,658 zooplank-tonic species(including subspecies) belonging to 206 families and 675 genera have been recorded from taxonomic identifications and literature,99 families,251 genera and 697 species belong to the Medusozoa,12 families,22 genera and 59 species to the Ctenophora,14 families,35 genera and 86 species to the pelagic Molluscs(Pteropoda and Heteropoda),8 families,89 genera and 416 species to the Ostracoda,51 families,156 genera and 908 species to the Copepoda,4 families,58 genera and 202 species to the Mysidcea,2 fami-lies,8 genera and 56 species to the Euphausiacea,8 families,22 genera and 105 species to the Decapoda,5 families,8 genera and 48 species to the Chaetognatha,5 families,26 genera and 81 species to the Tunicata.908专项“中国海洋生物种类名录和图谱”(908-ZC-II-02)和908专项海洋生物样品库;科技部基础工作专项“我国和邻近西北太平洋海洋生物物种编目和分布图集编制”(2006FY220700

基于多平台融合的Python教学实践与研究

我校针对“Python语言程序设计基础”教学过程中,学生基本学情情况,结合本校实际开课情况和人才培养方案。对Python教学构建以雨课堂,MOOC,Python123等平台相结合的融合教学模式。实践表明,该课程多平台融合教学模式,有效提高了学生学习的积极性,提高了教学质量。</jats:p

Determination of Hesperidin and Arctiin in Ganmao Jieduling Granules by HPLC

建立了HPLC法同时测定感冒解毒灵颗粒中的橙皮苷和牛蒡苷。采用C18色谱柱,以1%乙酸-甲醇(52︰48)为流动相,检测波长280nm。橙皮苷和牛蒡苷在7.5～150和5～100μg/ml浓度范围内线性关系良好,平均回收率为99.2%和99.6%,RSD为0.64%和0.79%。An HPLC method was established for the simultaneous determination of hesperidin and arctiin in Ganmao Jieduling granules. A C18 column was used with the mobile phase of 1% acetic acid-methanol（52︰48） at the detection wavelength of 280 nm. The calibration curves of hesperidin and arctiin were linear in the concentration ranges of 7.5-150 and 5-100 μg/ml. The average recoveries were 99.2% and 99.6%,with RSDs of 0.64% and 0.79%

Joint Doppler Frequency offset Mitigation and Channel Estimation for Underwater Acoustic ofDM

水声通信是近年来研究的热点,但由于水声信道本身的问题,如多径延迟、多普勒频移、信号衰减、窄带宽、时变等特点,使得水声通信传输数据速率低,传输误码率(bEr)高.正交频分复用(OfdM)在水声通信中具有很大优势,但它对载波频率偏移(CfO)敏感.基于循环前缀正交频分复用(CP-OfdM)的水声通信系统,对多普勒引起的CfO进行补偿,同时利用延迟-多普勒域中的稀疏性对水声双选信道进行压缩感知信道估计.通过计算机仿真和水池实验,验证了这种双选信道估计的有效性和可靠性.Underwater acoustic communication is a research hotspot in recent years.However,the problem of underwater acoustic channel itself,such as severe multipath delay,doppler shift,signal attenuation,narrow bandwidth,time-varying,led to low data rate and high rate of transmission error.Orthogonal frequency division multiplexing(ofDM) has a great advantage in the underwater acoustic communication.But it is sensitive to carrier frequency offset(CFO).We first discuss cyclic prefix orthogonal frequency division multiplexing(CP-ofDM) system model,then compensate for the CFO caused by the doppler and make CS-based channel estimation by exploiting sparsity in the delay-doppler domain.Through computer simulation and pool experiments verified the validity and reliability of this doubly selective channels estimation

Research Progress on Biosynthesis of Long Chain Fuel Alcohol

This paper briefly introduced the excellent properties of long chain alcohols as liquid fuels and reviewed the three main biosynthesis pathways of long chain alcohols, i.e. , Ehrlich pathway, non-fermentation pathway and photosynthetic pathway. The metho