食管癌组织中CuZn-SOD活性和表达的研究

[目的]通过对食管癌组织和癌旁组织中CuZn-SOD活性和表达检测,探讨CuZn-SOD活性和表达在食管癌发生、发展中的作用。[方法]CuZn-SOD活性和表达的检测分别采用DTNB直接法和Western Bloting方法。[结果]总SOD和CuZn-SOD的活性在食管癌组织和相应癌旁组织中的差异尚不能认为有统计学意义。30%食管癌组织中CuZn-SOD的表达明显高于癌旁组织。[结论]不同病例中表达水平结果不一致,CuZn-SOD的表达水平与肿瘤的发生或发展存在一定的联系

Use of Municipal Solid Waste Incinerator Ashes as Improved Material of Excavated Surplus Soils

Recently, the municipal solid waste discharged from the home is being increased. How to develop the technique for the decontamination and recycle from the viewpoint of environmental pollution and lack of the final disposal space from reclamation sites is becoming an important subject. In this paper, a mixture of the harmless municipal solid waste incinerator ashes with the Ariake clay has been applied to improvement of the soft ground, and the effect of subsidence restraint has also been investigated based on numerical simulations and model experiments using centrifuge model test

An Integrated Debice with Combination of Protein Denaturation, Reduction, Desalting and on-column Digestion for High Throughput Pretreatment of Proteins

For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion.For proteome research, "bottom-up" strategy is one of the most commonly used approaches, by which proteins are in turn denatured, reduced, alkylated, desaled and digested prior to multidimensional HPLC-ESI/MS/MS analysis. However, such a multistep sample pretreatment procedure mighet suffer from sample loss, contamination and long analysis time. Therefore, to solve these problems, it would be a better solution to develop a comprehensive on line protein pretreatment system including protein denaturation, redution, desalting and digestion

基于亲水作用机理富集糖肽的酰胺型整体柱及制备和应用

本发明涉及一种糖肽富集专用的酰胺型整体柱的制备和应用。所述的整体柱采用带有酰胺功能基团的单体和交联剂，加入适当比例的致孔剂和引发剂，通过快速光聚的方法在紫外透明石英毛细管中制备的整体柱。该类整体柱具有制作简单快速、结构稳定均一、亲水性较好、酰胺功能基团多等优点，本发明的整体柱可以实现对微量生物样品中糖肽的基于亲水作用机理的柱上富集。在生物样品糖蛋白预处理和在线糖蛋白分析平台的构建方面具有很好的实用价值和应用前景

An integrated protein pretreatment device as the interface of two-dimensional high performance liquid chromatography systography system for hai throuput huiman serum proteome analysis

An integrated protein pretreatment device as the interface of two-dimensional high performance liquid chromatography systography system for hai throuput huiman serum proteome analysi