EFFECT OF ELECTRON DONOR AND ACCEPTOR ON CAPACITIES OF AMMONIA-RESITANT FROM AZOTBACTER VINELANDII

电子供体连二亚硫酸钠、甲基紫精及电子受体亚甲蓝均能强烈抑制棕色固氮菌表达固氮活性 ,并引起该菌的抗氨阻遏能力减弱。适当提高氧压 ,能提高菌体的固氮活性近1 5 % ,但过高的氧分压反而抑制菌体的固氮活性。此外 ,提高氢分压能降低棕色固氮菌菌体内的还原电位 ,从而达到提高菌体抗氨阻遏能力的效果。Electronic donors dithionite as well as m et hyl viologen and it receptor methylene blue strongly inhibited activity of nitro gen fixation that Azotobacter vinelandii expressed,which ca used capacity of ammonia-resistant weaken greatly.About 15% of total activity f rom the whole cell were risen by increasing suitable oxygen atmosphere.Opposite, t he activity was inhibited by increasing over concentration of O 2 further.The r eduction potential in the inside environment from Azotobacter vinelan dii whole cell shifted to negative by increasing hydrogen pressure,whi ch resulted in its capacity of ammonia-resistant rose.福建省自然科学基金资助项目 (No . 960 0 6

Primary studies on electro-inducing Azotobacter vinelandii to express characteristics of ammonium-resistant

采用循环伏安技术电诱导棕色固氮菌成为抗氨阻遏的菌株 ,其菌株最高耐氨率可高达 8mMNH+ 4 左右。农田实验结果表明 ,直接施用该固氮菌于农田中 ,能使玉米及花生增产 5%～ 8%。菌体的抗氨阻遏能力与土壤土质及成分有着密切关系。Azotobacter vinelandii was electro-induced into the strain with ammonium-resistant by cyclic voltammetry, which the strain showed insensitive to come up to 8 mMNH + 4. The experimental results showed that increasing 5%～8%yields of corn and peanut were obtained after the strain is directly manured into the farm land. The degree of ammonium-resistant from the strain is tightly connected with quality and composition of the soil.福建省自然科学基金资助项目!(No .96 0 0 6 );; 固体表面物理化学国家重点实验室基金资助项