放水路の修景に関する研究：東静岡地区大谷川放水路を対象として

静岡文化芸術大学201

A Design of Wireless Sensor Networks Node

对无线传感器网络的特点进行了分析和总结。以低功耗、短距离多跳通信为出发点提出了系统设计思路和方法,讨论了系统设计中的关键技术和问题并给出相应的解决策略。在设计中采用了超低功耗的单片机并提出了超帧异步的通信方式。分析了系统设计上的一些问题,给出了相应的解决方法。This paper analyses and summarizes the characteristics of wireless sensor network,then presents the design scheme of the wireless sensor network node module based on low-energy consumption,multi-hop and short-haul communication.The paper expounds the system′s framework,hardware circuit selectivity,and protocol stack and surveillance software in detail.The hardware design adopts ultra-low power consumption MSP430 MCU as well as asynchronous MAC based on superframe structure.Finally the analysis by synthesis of some problems probably appeared is presented therefore the relevant strategies for resolving the problems are delivered accordingly.福建省重点科技计划项目(2005H041

Screening of Gongshisong's Active Sites for Anti-sports Fatigue

目的:筛选公石松抗运动性疲劳的活性部位。方法:用80%乙醇提取制备公石松浸膏,用水分散后依次用石油醚、氯仿、乙酸乙酯、正丁醇进行萃取,得各部位提取物。将70只小鼠随机分为空白对照(1%羧甲基纤维素钠,CMC-Na)组、阳性对照[大株红景天胶囊,590 mg/(kg·d)]组和公石松石油醚、氯仿、乙酸乙酯、正丁醇提取物组及水层组(分别记为TS、TL、TY、TZ、TW组),公石松提取物组小鼠均按2.5 g(生药)/(kg·d)ig给药,连续7 d,测定小鼠负重游泳力竭时间。将70只小鼠按上述方法分组给药,测定小鼠体内肝糖原、肌糖原含量和肝脏系数。将80只小鼠除按上述方法分组给药外增设模型(1%CMC-Na溶液)组,不负重游泳90 min后测定小鼠血清中乳酸、肌酸激酶及尿素氮含量。结果:与空白对照组比较,TS、TY、TZ组小鼠负重游泳力竭时间延长,TY、TZ、TW组小鼠体内肝糖原含量增加,TS、TW组小鼠体内肌糖原含量增加,模型组小鼠血清中尿素氮、乳酸、肌酸激酶含量增加(P<0.05或P<0.01);与模型组比较,TS、TY组小鼠血清中尿素氮含量减少,TZ组及TW组小鼠血清中乳酸含量减少,TS组小鼠血清中肌酸激酶含量减少(P<0.05或P<0.01)。结论:公石松的石油醚、正丁醇部位和水层具有较好的抗疲劳活性。OBJECTIVE:To screen Gongshisong's active sites for anti-sports fatigue. METHODS:Gongshisong extract was prepared with 80% ethanol extraction technology,and extracted with petroleum ether,chloroform,ethyl acetate and n-butyl alcohol after dispersed with water to obtain the extract. 70 mice were randomly divided into blank control group(1% sodium carboxymethylcellulose,CMC-Na),positive control group [Rhodiola wallichiana capsules,590 mg/(kg·d)],petroleum ether,chloroform,ethyl acetate and n-butyl alcohol extracts and aqueous layer of Gongshisong groups(TS,TL,TY,TZ,TW groups). Gongshisong extracts groups was given relevant medicine 2.5 g(crude drug)/(kg·d),ig,for consecutive 7 days. Exhaustion time of burden swimming test was detected. 70 mice were grouped according to above method,and the contents of liver glycogen,muscle glycogen and the coefficient of liver were tested in mice. 80 mice were grouped according to above method,and model group was established additionally(1% CMC-Na). The contents of lactic acid(LA),creatine kinase(CK)and urea nitrogen(BUN)in serum of mice were determined after 90 minutes of unburden swimming. RESULTS:Compared with blank control group,exhaustion time of burden swimming mice in TS,TY and TZ groups prolonged;the content of liver glycogen increased in TY,TZ and TW groups;the content of muscle glycogen increased in TS and TW groups;the contents of BUN,LA and CK in mice increased in model group(P<0.05 or P<0.01). Compared with model group,the serum content of BUN in mice decreased in TS and TY groups;that of LA in mice decreased in TZ and TW groups;that of CK in mice decreased in TS group(P<0.05 or P<0.01).CONCLUSIONS:The petroleum ether and n-butanol extract site and water layer of Gongshisong are good anti-fatigue active sites.解放军第175医院青年苗圃基金资助项目(No.13Y017

Association between Methylenetetrahydrofolate Reductase Gene Single Nucleotide Polymorphisms and Lower Extremity Atherosclerotic Disease

目的:研究汉族人群中亚甲基四氢叶酸还原酶(methylene-tetrahydrofolate reductase,MTHFR)基因多态性与下肢动脉粥样硬化(lower extremity atherosclerotic disease,LEAD)的相关性。方法:收集福建省闽南地区384例(LEAD者224例,健康者160例)的临床资料及外周血;LEAD检查采用踝肱指数(ABI)、趾肱指数(TBI)、多普勒彩超和其他影像学检查等手段;选取MTHFR基因rs1801133、rs1801131、rs2274976、rs4846048、rs3737966、rs1537515、rs4846049、rs3834044、rs13306561和rs3737964等10个单核苷酸多态性(single nucleotide polymorphisms,SNP)位点进行基质辅助激光解吸电离飞行时间质谱分析技术(matrix-assisted laser desorption ionization-time of flight,MALDI-TOF)的基因分型。结果:10个SNP位点均符合HardyWeinberg平衡;rs4846048与rs3737966等37个位点之间存在明显连锁不平衡现像(D'均大于0.9);MTHFR基因GCCTCGGAAT单倍型在LEAD和正常组的分布差异有统计学意义(P=0.02);等位基因频率的χ~2检验显示rs1801131(OR=1.287);rs4846048(OR=1.844,P=0.02);rs3737966(OR=1.339);rs4846049(OR=1.314)和rs3737964(OR=1.522);且rs4846048位点的趋势χ~2检验(cochran-armitage trend test,TREND)、显性基因检验(Dominant gene action test,DOM)均显示LEAD与正常组之间分布频率的差异有统计学意义(P 0. 9) and so on. There were significant differences( P = 0. 02) in GCCTCGGAAT haplotypes of MTHFR gene groups between LEAD cases and the normal groups. The results from chi-square test of allele frequencies suggested rs1801131( OR = 1. 287),rs4846048( OR = 1. 844,P = 0. 02),rs3737966( OR =1. 339),rs4846049( OR = 1. 314) and rs3737964( OR = 1. 522). Significant differences( P < 0. 05) were observed between LEAD and the normal groups in Cochran- Armitage trend test and Dominant gene action test of rs4846048. Conclusion: The SNP of rs1801131,rs4846048,rs3737966,rs4846049 and rs3737964 might be associated with the susceptibility of LEAD,and rs4846048 gene mutation might serve as a risk factor for LEAD in the community-based population.2013年度南京军区医学科技创新课题(编号:MS098);; 解放军第175医院青年苗圃基金项目(编号:13Y015

Effects of Hemocoagulase Agkistrodon for Hemostasis in Surgical Tresis Vulnus: A Meta-analysis

目的:评价尖吻蝮蛇血凝酶(HCA)对手术创口的止血效果。方法:计算机检索Pub MEd、CbM、中国知网、维普和万方等数据库,收集采用HCA治疗手术创口出血的临床随机对照试验(rCT),并追溯纳入研究的参考文献。由2位评价者按照纳入排除标准独立筛选文献、提取资料和评价质量后,采用rEV MAn 5.2软件进行META分析。结果:纳14篇文献,共1 382个患者。META分析结果显示:与对照组比较,HCA可明显减少患者手术切口创面出血量[Md=-1.67,95%CI(-2.04,-1.30),P<0.000 1],减少术后引流量[Md=-19.10,95%CI(-25.96,-12.25),P<0.000 01],减少切口单位面积出血量[Md=-0.05,95%CI(-0.08,-0.03),P<0.000 01],缩短止血时间[Md=-50.67,95%CI(-74.85,-26.49),P<0.000 1]。结论:术前给予尖吻蝮蛇血凝酶能明显减少手术患者切口创面出血量、术后引流量和切口单位面积出血量,并缩短止血时间,但本研究仍有局限性,需大样本、多中心、随机对照临床试验进一步证实。Objective: To review the effects of hemocoagulase agkistrodon( HCA) for hemostasis in surgical tresis vulnus.Methods: Databases including Pub Med,CBM,CNKI,VIP and Wanfang were searched electronically to collect literature published.Randomized controlled trials( RCTs) were identified about HCA for hemostasis in surgical tresis vulnus.References of the included studies were also retrieved.Two reviewers independently screened literature according to the inclusion and exclusion criteria,extracted data,and assess the quality of the included studies.Then Meta-analysis was performed using Rev Man 5.2 software.Results: Fourteen trials involved 1 382 patients were included.The results of Metaanalysis indicated that,using HCA for hemorrhagic volume in surgical tresis vulnus[MD =- 1.67,95% CI(- 2.04,- 1.30),P < 0.000 1 ],drainage volume after the operation[MD =- 19.10,95% CI(- 25.96,- 12.25),P <0.000 01],hemorrhagic volume per square unit[MD =- 0.05,95% CI(- 0.08,- 0.03),P < 0.000 01] significantly decreased in comprison with blank control,hemostatic time[MD =- 50.67,95% CI(- 74.85,- 26.49),P < 0.000 1]were significantly shorten with blank control.Conclusion: HCA administered preoperatively could significant reduce the hemorrhagic volume,drainage volume after the operation,hemorrhagic volume per square unit and shorten hemostatic time compared with blank control in surgical patients.However,due to limitations of the included studies,the clinical effects need to be confirmed by large multicenter randomized controlled trials

Research on the microbial limit verification test of tacrolimus capsules

目的按2010版《中国药典》的要求探讨他克莫司胶囊微生物限度检测方法。方法选取5种具有代表性的微生物菌悬液,用常规法、稀释法、膜过滤法或其相结合的方法分别对他克莫司胶囊进行微生物验证试验。结果细菌总数用膜过滤法稀释1∶10,霉菌和酵母菌用膜过滤法稀释1∶1 000,检测后回收率均能达到70%以上。结论避免采用单一常规法或膜过滤法进行微生物限度检测,使实验科学合理,提高工作效率,更加规范性。Objective To research the microbial limit verification test of tacrolimus capsules according to the requirements of the 2010-edition Chinese Pharmacopoeia.Methods Five types of representative microbial bacterial suspension were selected and then microbial verification tests on tacrolimus capsules through the conventional method,the dilution method,the membrane filtration method or a combination of the above methods respectively were carried out.Results Both of the recovery rates were more than 70% when the total number of bacterial was detected by the membrane filtration method as per 1∶ 10 and when mould and yeast were detected by the membrane filtration method as per 1∶ 1 000.Conclusion Microbial limit test through the single conventional method or membrane filtration method is avoided so as to ensure scientific and reasonable experiment,improve work efficiency and achieve normalization

Anti-fatigue Action of Vitamins with Minerals Tablets(21)

目的研究多维元素片(21)抗疲劳作用。方法将昆明种雄性小鼠40只随机分为空白对照组,多维元素片(21)低(0.21 g.kg-1)、中(0.42 g.kg-1)、高(0.84 g.kg-1)剂量组,各10只。空白对照组给予等体积,纯化水。连续灌胃给药7 d,观察多维元素片(21)对小鼠负重游泳时间、肝糖原和血乳酸的影响。结果与空白对照组比较,多维元素片(21)各剂量组小鼠负重游泳时间延长,血乳酸值降低,肝糖原含量升高,以中剂量组最明显。多维元素片(21)低剂量组小鼠血乳酸值降低,小鼠肝糖原含量增加显著(P<0.05);多维元素片(21)中剂量组小鼠肝糖原含量增加显著(P<0.05),小鼠游泳时间有效延长和小鼠血乳酸值降低(P<0.01);多维元素片(21)高剂量组小鼠游泳时间延长,小鼠血乳酸值降低作用显著(P<0.05),小鼠肝糖原含量有效增加(P<0.01)。结论多维元素片(21)具有抗疲劳作用。Objective To study the anti-fatigue effect of vitamins with mineral tablets(21).Methods The male Kunming mice were randomly divided into blank control group and three groups of vitamins with mineral tablets(21) at doses of 0.21,0.42 and 0.84 g·kg-1.The blank control group was given commensurable distilled water.The four groups were treated by gavage for continuous 7 days,and the weight-loaded swimming time,blood lactic acid(BLA) and the level of liver glycogen(LG) were evaluated.Results Compared with the blank control group,the mice in the groups of vitamins with minerals tablets(21)at all doses showed longer swimming time,lower contents of BLA,higher LG,especially for the middle-dose.Vitamins with minerals tablets(21) low-dose group of mice blood lactate values decreased and mouse liver glycogen content increased significantly(P<0.05);Vitamins with minerals tablets(21) middle-dose group in glycogen content increased significantly(P<0.05),effectively extend the swimming time in mice and mouse blood lactate values decreased(P<0.01);Vitamins with minerals tablets(21) high-dose group extend the swimming time in mice and mouse blood lactate values decreased(P<0.05),effectively increased the glycogen content in mice(P<0.01).Conclusion Vitamins with minerals tablets(21) has strong anti-fatigue capability

戊型肝炎病毒ORF2片段在毕赤酵母中的分泌表达及活性鉴定

　为了寻求新型表达系统来研制戊型肝炎病毒基因工程疫苗,利用Pichiapastoris表达系统表达戊型肝炎病毒(HEV)结构区ORF2基因.利用PCR扩增HEVORF2基因,然后将ORF2基因按正确的阅读框融合到Pichiapastoris分泌型表达载体pPIC9K的а因子信号肽编码序列3′端,重组表达载体在电击转化毕赤酵母,经过含G418的营养缺陷型培养基(RDB)筛选、重组酵母基因组总DNA进行PCR鉴定后,证实HEVORF2基因已经整合到酵母基因组中并得到重组转化子.表型鉴定后对G418具有不抗性的重组菌株诱导表达,用双抗体夹心法ELISA筛选了表达外源蛋白的重组菌株,再经SDS PAGE与Westernblot证实ORF2基因在Pichiapastoris中实现了分泌表达,而且重组蛋白具有较强的特异性与生物学活性;同时用双抗体夹心法证实在Pichiapastoris表达的上清中存在衣壳蛋白聚体

红色荧光蛋白在毕赤酵母中的高效表达

报道了红色荧光蛋白(DsRFP)在毕赤酵母中的高水平表达.利用PCR技术从YEpFLAG- 1 -DsRFP扩增出DsRFP编码序列,克隆到毕赤酵母胞内表达载体pPIC3. 5K构建重组表达载体pPIC3. 5K- DsRFP,电击转化进毕赤酵母,G418 RDB平板双重筛选后获得重组转化子,经MM/MD平板培养与PCR鉴定,重组子全部为HIS+MUT+表型.重组菌株诱导表达48h后获得了高效表达,摇瓶中表达水平达到细胞内总蛋白的12%,表达量达到1. 8g/L.经硫酸铵分级沉淀,DEAE- 5PW阴离子交换柱层析与S- HyperD阳离子交换柱层析后获得电泳纯蛋白.说明我们建立的毕赤酵母表达应用平台具有高效表达外源蛋白的能力